Plantlet Regeneration through Indirect Organogenesis of Flame Gold Tree (Koelreuteria elegans Laxm.)

الموضوعات : مجله گیاهان زینتی

Rameshwar Groach ¹ (Department of Botany, Kurukshetra University, Kurukshetra 136119 (India))
Muzafar Hussain Dar ² (Department of Botany, Kurukshetra University, Kurukshetra 136119 (India))
Kartar Chand Badgal ³ (Principal, Govt. Degree College, Hiranagar, Kathua)
Priyanka Pal ⁴ (Department of Botany, Kurukshetra University, Kurukshetra 136119 (India))
Narender Singh ⁵ (Department of Botany, Kurukshetra University, Kurukshetra 136119 (India))
Kuldeep Yadav ⁶ (Department of Botany, Kurukshetra University, Kurukshetra 136119 (India))

تاريخ الإرسال : 23 السبت , شوال, 1436 تاريخ التأكيد : 23 السبت , شوال, 1436 تاريخ الإصدار : 06 الإثنين , ذو القعدة, 1435

الکلمات المفتاحية: Auxins, Cytokinins, Koelreuteria elegans, Ornamental tree,

ملخص المقالة :

Koelreuteria elegans, popularly known as “Flame Gold” is an ornamental tree. In vitro callus induction and regeneration from various explants (eaf segments and cotyledonary leaf) were studied on modified MS medium. The highest callus induction rate (80%) and multiplication was obtained in 2 mg/l 2,4-D from leaf segments. Calli transferred in 1.5 mg/l BAP resulted in efficient shoot regeneration (70%) and development (4.35 shoots). MS half strength medium supplemented with 0.2 mg/l NAA reported 80% rooting after 21 days of implantation. Mostly, the roots were long and healthy. Plants were successfully transferred in sterilized mixture of vermiculite: soil: sand (3:1:1) with 65% survival rate under field conditions. The in vitro regenerated plantlets were hardened and acclimatized successfully.

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