Mutation Induction in Chrysanthemum Cut Flowers Using Gamma Irradiation Method
الموضوعات : مجله گیاهان زینتیMohammad Reza shafiei 1 , Abdollah Hatamzadeh 2 , Pejman Azadi 3 , Habibollah Samizadeh Lahiji 4
1 - PH.D. Student in Horticultuer Department of Horticultural Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran. P.O. Box: 41635-1314
2 - Department of Horticultural Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran. P.O. Box: 41635-1314
3 - Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Agricultural Research, Education and Extension Organization (AREEO), Iran| Ornamental Plant Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Iran
4 - Department of Agronomy Plant Breeding, Faculty of Agriculture Science, University of Guilan
الکلمات المفتاحية: Gamma ray, Chrysanthemum, Mutagenesis, Radiation,
ملخص المقالة :
Chrysanthemum is one of the ornamental plants that cultivated widely in worldwide. This flower is one of the most important cut and pot flowers in the world due to its high diversity of colors and shapes. The creation of mutation is an important method for the production of new cultivars, and many cultivars have been produced through spontaneous and induced mutations. In this study, explants of leaf pieces from three most cultivated chrysanthemum varieties were irradiated with different gamma rays. This experiment was conducted as factorial in randomized complete block design with three replications. The results showed that an appropriate dose of gamma ray to produce a mutation in the varieties used in this experiment, is 25 G-ray. The obtained results showed that the 25 G-ray in purple cultivar produced the greatest change in petal color with a mutation rate of 54.56%. Meanwhile, the maximum number of new colors belonged to the purple group. Also, in the pink cultivar, the highest number of colored flowers was observed with a change of 32.11% in the 25 G-ray treatment. Based on the results of this study, four new cultivars will be introduced to the Iranian flower industry as a new cultivar.
Azadi, P., Bagheri, H., Nalousi, A.M., Nazari, F. and Chandler, S.F. 2016. Current status and biotechnological advances in genetic engineering of ornamental plants. Biotechnology Advances, 34 (6): 1073-1090.
Banerji, B.K., Datta, S.K. 2002. Induction and analysis of gamma ray-induced flower head shape mutation in Lalima chrysanthemum (Chrysanthemum morifolium). Indian Journal of Agricultural Sciences, 71(1): 6-10.
Datta, S.K., Misra, P. and Mandal, K.A. 2005. In vitro mutagenesis – a quick method for establishment of solid mutant in Chrysanthemum. Current Science, 88 (1): 155-158.
Dowrick, G.J. and El-Bayoumi, A. 1966. The induction of mutations in Chrysanthemum using X- and gamma radiation. Euphytica, 15: 204-210.
Ichikawa, S., Yamakawa, K., Sekiguchi, F. and Tatsuno, T. 1970. Variation in somatic chromosome number found in radiation-induced mutants of Chrysantemum morifolium Hrmsl. cv. ‘Yellow Delaware’ and ‘Delaware’. Radiation Botany, 10: 557-562.
Misra, P., Datta, S.K. and Chakrabarty, D. 2003. Mutation in flower colour and shape of Chrysanthemum morfolium induced by γ-radiation. Biologia Plantarum, 47 (1): 153-156.
Nagatomi, S. and Degi, K. 2009. Mutation breeding of Chrysanthemum by famma field irradiation and in vitro culture. Food and Agriculture Organization of United Nations, Rome, 258-261.
Nagatomi, S., Miyahira, E. and Degi, K. 2000. Induction of flower mutation comparing with chronic and acute gamma irradiation using tissue culture techniques in Chrysanthemum morifolium Ramat. Acta Horticulturae, 508: 69-73.
Nakagawa, H. 2009. Induced mutation in plant breeding and biological researches in Japan. Food and Agriculture Organization of United Nations, Rome, 48-54.
Okitsu, N., Noda, N., Chandler, S. and Tanaka, Y. 2018. Hand book of plant breeding 11. https://doi.org/10.1007/978-3-319-90698-03.
Patil, U.H., Karale, A.R., Katwate, S.M. and Patil, M.S. 2017. Mutation breeding in chrysanthemum (Dendrathema grandiflora T.). Journal of Pharmacognosy and Phytochemistry, 6 (6): 230-232.
Predieri, S. and Virgilio, N.D. 2007. Invitro mutagenesis and mutant multiplication. Protocols for Micropropagation of Woody Trees and Fruits. pp 323-333.
Schum, A. 2003. Mutation breeding in ornamentals: An efficient breeding method. Acta Horticulturea, 612: 47-60.
Szarejko, B. and Foster, P. 2007. Doubled haploidy and induced mutation. Euphytica, 158: 359-370.
Yamaguchi, H., Shimizu, A., Degi, K. and Morishita, T. 2008. Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in Chrysanthemum. Breeding Science, 58: 331-335.
Yesmin, S., Hashem, A., Das, K.C., Hasan, M.M. and Islam, M.S. 2014. Efficient in vitro regeneration of chrysanthemum (Chrysanthemum morifolium Ramat.) through nodal explant culture. Nuclear Science and Applications, 23(1&2): 47-50.
