Effects of foliar application of salicylic acid on pigments, photosynthesis, ion leakage, compatible osmolytes and antioxidant enzymes of rapeseed (Brassica napus L.) under chilling stress
الموضوعات :
Hamed Sepahvand
1
,
Hamid Reza Eisvand
2
,
Omid Ali Akbarpour
3
1 - Department of Plant Production Engineering and Genetics, Lorestan University, Iran
2 - Department of Plant Production and Genetic Engineering, Lorestan University
3 - Department of Agronomy and Plant Breeding, Lorestan University, Iran
الکلمات المفتاحية: Rapeseed, Proline, Soluble sugars, Sowing date, chilling stress,
ملخص المقالة :
Chilling stress inhibits yield of rapeseed. Salicylic acid (SA) can improve plants’ resistance to chilling through modification of many physiological mechanisms. In the present study, foliar of SA was investigated on some physiological characteristics of rapeseed under chilling stress in farm. Treatments included sowing date (conventional date, late September, and late 15-day delay, which represents chilling stress) and SA spraying including no spraying, 0, 100, 200, 300, and 400 µM. Findings revealed that conventional sowing date + 100 µM SA resulted in the maximum chlorophyll, anthocyanins, and proline contents while late sowing date+ 100 µM SA resulted in the maximum flavonoids and soluble sugar. The highest catalase activities were recorded for the plants treated with 100 and 400 µM SA, but delayed sowing + 200 µM SA led to the highest activity of superoxide dismutase. Non-application of SA + conventional date showed the highest ion leakage. Applying low concentration of SA to the rapeseed plants sown in the favorable (conventional) sowing date seems to improve their chlorophyll and anthocyanins contents, and increasing the SA in delayed sowing seems to reduce the adverse effects of chilling stress. In the case of flavonoids and soluble sugars, applying 100 µM SA in the delayed sowing group compensated for the negative effects of low temperature compared with the control, improving the plant’s resistance to this stress. In sum, the most favorable performance in most characteristics of the rapeseed was obtained with conventional sowing date along with applying 100 µM SA.
Abdelhameed, R.E., A.A.H. Abdel Latef and R.S. Shehata. 2021. Physiological responses of salinized fenugreek (Trigonella foenum-graecum L.) plants to foliar application of salicylic acid. Plants, 10, (4) 657.
Adamski, J.M., L.M.G. Rosa, C.R.D. Menezes Peixoto, C.L. Pinheiro, J.P. Fett and R.A. Sperotto. 2020. Photosynthetic activity of indica rice sister lines with contrasting cold tolerance. Physiology and molecular biology of plants, 26, 955-964.
Ahmad, F., A. Kamal, A. Singh, F. Ashfaque, S. Alamri and M.H. Siddiqui. 2020. Salicylic acid modulates antioxidant system, defense metabolites, and expression of salt transporter genes in Pisum sativum under salinity stress. Journal of Plant Growth Regulation, 1-14.
Ali, B. 2021. Salicylic acid: An efficient elicitor of secondary metabolite production in plants. Biocatalysis and Agricultural Biotechnology, 31, 101884.
Artés–Hernández, F., F.D. Miranda-Molina, T.V. Klug and G.B. Martínez–Hernández. 2022. Enrichment of glucosinolate and carotenoid contents of mustard sprouts by using green elicitors during germination. Journal of Food Composition and Analysis, 110, 104546.
Bates, L.S. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39, 205-207.
Bhattacharya, A. 2022. Physiological processes in plants under low temperature stress. Springer.
Biareh, V., F. Shekari, S. Sayfzadeh, H. Zakerin, E. Hadidi, J.G.T. Beltrão and A. Mastinu. 2022. Physiological and qualitative response of Cucurbita pepo L. to salicylic acid under controlled water stress conditions. Horticulturae, 8, (1) 79.
Cakmak, I. and Horst, W. J. 1991 Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum, 83: 463-468.
Chavoushi, M., F. Najafi, A. Salimi and S.A. Angaji. 2020. Effect of salicylic acid and sodium nitroprusside on growth parameters, photosynthetic pigments and secondary metabolites of safflower under drought stress. Scientia Horticulturae, 259, 108823.
Davami, P., M.A. Fazel, S. Lak, D. Habibi and A. Mozaffari. 2022. Antioxidant enzyme activities and grain yield of rapeseed (Brassica napus L.) of response to late-season drought and sowing date. Romanian Agricultural Research, 39, 67-78.
Duan, X., Z. Zhu, Y. Yang, J. Duan, Z. Jia, F. Chen, Z. Sang and L. Ma. 2021. Salicylic acid regulates sugar metabolism that confers freezing tolerance in Magnolia wufengensis during natural cold acclimation. Journal of Plant Growth Regulation, 1-9.
Eisvand, H.R., N. Fathi and D. Goudarzi. 2015. Effects of some PGRs on seedling emergence and CAT and POD activity of maize under low temperature stress. Iranian Journal of Plant Physiology, 5(3), 1393-1402. doi: 10.30495/ijpp.2015.539667
Farhangi-Abriz, S. and K. Ghassemi-Golezani. 2018. How can salicylic acid and jasmonic acid mitigate salt toxicity in soybean plants? Ecotoxicology and environmental safety, 147, 1010-1016.
Ghassemi-Golezani, K., N. Hassanzadeh, M.R. Shakiba and B. Esmaeilpour. 2020. Exogenous salicylic acid and 24-epi-brassinolide improve antioxidant capacity and secondary metabolites of Brassica nigra. Biocatalysis and Agricultural Biotechnology, 26, 101636.
Giannopolitis, C. N. and Ries, S. K. 1977 Superoxide dismutase occurrence in higher plants. Plant Physiology 59: 309 -314.
Guo, Q., X. Li, L. Niu, P.E. Jameson and W. Zhou. 2021. Transcription-associated metabolomic adjustments in maize occur during combined drought and cold stress. Plant Physiology, 186, (1) 677-695.
Hasanuzzaman, M., M.B. Bhuyan, F. Zulfiqar, A. Raza, S.M. Mohsin, J.A. Mahmud, M. Fujita and V. Fotopoulos. 2020. Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial role of a universal defense regulator. Antioxidants, 9, (8) 681.
Heydarnejadiyan, H., A. Maleki and F. Babaei. 2020. The effect of zinc and salicylic acid application on grain yield, essential oil and phytochemical properties of fennel plants under drought stress. Journal of Essential Oil Bearing Plants, 23, (6) 1371-1385.
Hussain, H.A., S. Hussain, A. Khaliq, U. Ashraf, S.A. Anjum, S. Men and L. Wang. 2018. Chilling and drought stresses in crop plants: implications, cross talk, and potential management opportunities. Frontiers in plant science, 9, 393.
Ignatenko, A., V. Talanova, N. Repkina and A. Titov. 2019. Exogenous salicylic acid treatment induces cold tolerance in wheat through promotion of antioxidant enzyme activity and proline accumulation. Acta Physiologiae Plantarum, 41, 1-10.
Jahani, F., H.R. Tohidi-Moghadam, H.R. Larijani, F. Ghooshchi and M. Oveysi. 2021. Influence of zinc and salicylic acid foliar application on total chlorophyll, phenolic components, yield and essential oil composition of peppermint (Mentha piperita L.) under drought stress condition. Arabian Journal of Geosciences, 14, 1-12.
Kochert, G. 1978. Carbohydrate determination by phenol sulfuric acid method. In: Hellebust J A, Craigie JS (Eds), Handbook of physiological methods. Cambridge. UK: Cambridge University Press, pp. 95-97.
Kohli, S.K., N. Handa, A. Sharma, V. Gautam, S. Arora, R. Bhardwaj, M.N. Alyemeni, L. Wijaya and P. Ahmad. 2018. Combined effect of 24-epibrassinolide and salicylic acid mitigates lead (Pb) toxicity by modulating various metabolites in Brassica juncea L. seedlings. Protoplasma, 255, 11-24.
Krizek, D. T., Britz, S. J. and Mirecki, R. M. 1998 Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of cv. new red fire lettuce. Physiologia Plantarum 103: 1-7.
Lei, Y.A.N., T. Shah, Y. Cheng, L.Ü. Yan, X.K. Zhang and X.L. Zou. 2019. Physiological and molecular responses to cold stress in rapeseed (Brassica napus L.). Journal of Integrative Agriculture, 18, (12) 2742-2752.
Li, B. and W. Wang. 2021. Salicylic acid induces tolerance of Vitisriparia× V. labrusca to chilling stress by altered photosynthetic, antioxidant mechanisms and expression of cold stress responsive genes. Plant Signaling & Behavior, 16, (11) 1973711.
Lichten Thaler H.K. and F. Babani. 2000. Detection of photosynthetic activity and water stress by imaging the red chlorophyll fluorescence. Plant Physiology and Biochemistry. 38, 889-895.
Liu, X., Y. Zhou, J. Xiaoand and F. Bao. 2018. Effects of chilling on the structure, function and development of chloroplasts. Frontiers in plant science, 9, 1715.
Lutts, S., J.M. Kint and J. Bouharmont. 1996. NaCl-induced senescence in leaves of rice (Oriza sativa L.) cultivars differing in salinity resistance. Annals of Botany. 78, 389-398.
Min, K. and S.R. Lee. 2021. Exogenous Salicylic Acid Alleviates Freeze-Thaw Injury of Cabbage (Brassica oleracea L.) Leaves. Sustainability, 13, (20) 11437.
Nesterova, N., O. Pareniuk, V. Illienko, Y. Ruban, K. Shavanova and N. Shpyrka. 2019. Physiological Reactions in Cereals Family Avena Sativa L. and Avena Nuda L., Caused by Low-Temperature Stress Factors. In 2019 IEEE 39th International Conference on Electronics and Nanotechnology (ELNANO) (pp. 502-506). IEEE.
Parveen, A., M. Arslan Ashraf, I. Hussain, S. Perveen, R. Rasheed, Q. Mahmood, S. Hussain, A. Ditta, A. Hashem, A.B.F. Al-Arjani and A.A. Alqarawi. 2021. Promotion of growth and physiological characteristics in water-stressed Triticum aestivum in relation to foliar-application of salicylic acid. Water, 13, (9) 1316.
Qu, X., H. Wang, M. Chen, J. Liao, J. Yuan and G. Niu. 2019. Drought stress–induced physiological and metabolic changes in leaves of two oil tea cultivars. Journal of the American Society for Horticultural Science, 144, (6) 439-447.
Rezaie, R., B. Abdollahi Mandoulakani and M. Fattahi. 2020. Cold stress changes antioxidant defense system, phenylpropanoid contents and expression of genes involved in their biosynthesis in Ocimum basilicum L. Scientific reports, 10, (1) 1-10.
Saleem, M., Q. Fariduddin and T. Janda. 2021. Multifaceted role of salicylic acid in combating cold stress in plants: A review. Journal of Plant Growth Regulation, 40, 464-485.
Shafiei, N., E. Khaleghi and N. Moallemi. 2019. Effect of salicylic acid on some morphological and biochemical characteristics of Olive (Olea europaea cv.‘Konservalia’) under water stress. Plant Productions, 42, (1) 15-30.
Shaki, F., H.E. Maboud and V. Niknam. 2018. Growth enhancement and salt tolerance of Safflower (Carthamus tinctorius L.), by salicylic acid. Current Plant Biology, 13, 16-22.
Sharma, P., M.M.M. Sharma, A. Patra, M. Vashisth, S. Mehta, B. Singh, M. Tiwari and V. Pandey. 2020. The role of key transcription factors for cold tolerance in plants. In Transcription factors for abiotic stress tolerance in plants, 123-152. Academic Press.
Shenavaei Zare, M., M. Armin and H. Marvi. 2021. Physiological responses of cotton to stress moderator application on different planting date under saline conditions. Iranian Journal of Science and Technology, Transactions A: Science, 45, (1) 11-25.
Shin, H., K. Min and R. Arora. 2018. Exogenous salicylic acid improves freezing tolerance of spinach (Spinacia oleracea L.) leaves. Cryobiology, 81, 192-200.
Solanki Mital, V., K. Trivedi Sandhya, U.K. Kandoliya and B.A. Golakiya. 2018. Effect of exogenous application of salicylic acid on antioxidative enzymes in black gram (Vigna mungo (L.) Hepper) irrigated with saline water. International Journal of Chemical Studies, 6, 2107-2116.
Soliman, M.H., A.A. Alayafi, A.A. El Kelish and A.M. Abu-Elsaoud. 2018. Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes. Botanical studies, 59, (1) 1-17.
Song, W., P. Zhang, H. Zhang, Q. Zhang, M. Ning, X. Zhao and C. Shan. 2022. A low concentration of exogenous salicylic acid enhances cold tolerance in Hami melons (Cucumis melo var. saccharinus) by modulating salicylic acid-response CmGST genes. Postharvest Biology and Technology, 193, 112034.
Tajik, S., F. Zarinkamar, B.M. Soltani and M. Nazari. 2019. Induction of phenolic and flavonoid compounds in leaves of saffron (Crocus sativus L.) by salicylic acid. Scientia Horticulturae, 257, 108751.
Tavallali, V., H. Gholami and O. Espargham. 2020. Biological and pharmacological activities of essential oils of Ocimum basilicum L. grown with Zn-salicylic acid nano-complex. Journal of Applied Botany and Food Quality, 93, 26-33.
Teymoori, M., M.R. Ardakani, A.H.S. Rad, M. Alavifazel and P.N. Manavi. 2020. Seed yield and physiological responses to deal with drought stress and late sowing date for promising lines of rapeseed (Brassica napus L.). International Agrophysics, 34(3) 321-331.
Wagner, G. J. (1979) Content and vacuole/extra vacuole distribution of neutral sugars, free amino acids, and anthocyanins in protoplast. Plant Physiology 64: 88-93.
Wang, W., X. Wang, Z. Lv, A. Khanzada, M. Huang, J. Cai, Q. Zhou, Z. Huo and D. Jiang. 2022. Effects of cold and salicylic acid priming on free proline and sucrose accumulation in winter wheat under freezing stress. Journal of Plant Growth Regulation, 41, (6) 2171-2184.
Xu, H., C. Huang, X. Jiang, J. Zhu, X. Gao and C.Yu. 2022. Impact of cold stress on leaf structure, photosynthesis, and metabolites in Camellia weiningensis and C. oleifera seedlings. Horticulturae, 8, (6) 494.
Zand-Silakhoor, A., H. Madani, H.H. Sharifabad, M. Mahmoudi and G. Nourmohammadi. 2022. Influence of different irrigation regimes and planting times on the quality and quantity of calyx, seed oil content and water use efficiency of roselle (Hibiscus sabdariffa L.). Grasas y Aceites, 73, (3) e472-e472.
Zhu, Z. H., A. Sami, Q. Xu, L.L.Wu, W.Y.Zheng, Z.P. Chen and K.J. Zhou. 2021. Effects of seed priming treatments on the germination and development of two rapeseed (Brassica napus L.) varieties under the co-influence of low temperature and drought. Plos one, 16, (9) e0257236.
