Change in the content of bioactive pharmaceutical-industrial compounds under the influence of hormonal treatments In Euphorbia trigona Mill.

الموضوعات :

Hakimeh Rezayi ¹ , Aryan Sateei ² , Tahereh A. Aghajanzadeh ³ , Mehdi Ebadi ⁴

1 - Department of Biology, Faculty of science, Islamic Azad University, Gorgan Branch, Gorgan, Iran
2 - Department of Biology, Faculty of Science, Islamic Azad University Branch Gorgan, Gorgan, Iran
3 - Department of Plant Sciences, Faculty of Science, University of Mazandaran, Babolsar, Iran
4 - Department of Chemistry, Faculty of science, Islamic Azad University, Gorgan Branch, Gorgan, Iran

تاريخ الإرسال : 27 الإثنين , ربيع الثاني, 1444 تاريخ التأكيد : 15 الإثنين , رجب, 1444 تاريخ الإصدار : 05 السبت , ربيع الأول, 1444

الکلمات المفتاحية: Indole Butyric Acid, Naphthalene acetic acid, GC-MS, Indole Acetic Acid, Growth Regulator,

ملخص المقالة :

In this study, the effects of auxin and gibberellin on Euphorbia trigona plants, grown in a complete randomized blocks design under greenhouse conditions were investigated. One-minute pretreatments including auxin regulators NAA, IBA and IAA, (0, or 500 mg / l), followed by treatments containing IAA, IBA and NAA, 250 mg / l, and GA treatments,250 mg/l, without any pretreatments were utilized on 2 month cuttings. After 4 months, fresh and dry weight of shoots and roots, and shoot total terpenoid content were measured. In some samples, shoot extract was analyzed using GC-MS. Fresh and dry weight of shoot did not show any sensitivity to the treatments and pretreatments. NAA treatment had the most positive effect on root fresh and dry weight. The highest significant increase in terpenoid content was observed in NAA treatment and NAA and IBA pretreatments. GC-MS analysis showed that the type of chemical compounds in the extract were mainly including sesquiterpenes, diterpenes, fatty acids, esters and steroids with various medicinal and antimicrobial properties as well as industrial applications. Four compounds including bis-2-ethylhexyl phthalate, hexadecanoic acid, 6,10,14-trimethyl-2-pentadecanone (Hexahydrofarnesyl acetone) and neophytadiene were observed in all treatments and controls with different percentages: Bis-2-ethylhexyl phthalate in NAA treatment 49.31%, in control 9.59%; Hexadecanoic acid in NAA treatment 2.73%, in control 2.12%; 6,10,14-trimethyl-2-pentadecanone, in NAA treatment 3.16%, in control, 1.42%, Neophytadiene in control 0.94% and in gibberellin treatment 1.64%. This comparison prominently showed that hormone utilization could have a positive effect on terpenoids and fatty acids anabolic pathways in Euphorbia trigona.

المصادر:

Abd-Elhakim, Y. M., M. Abdo Nassan, G. A. Salem, A. Sasi, A. Aldhahrani, K. Ben Issa and A. Abdel-Rahman Mohamed. 2019. Investigation of the in-vivo cytotoxicity and the in silico-prediction of MDM2-p53 inhibitor potential of euphorbia peplus methanolic extract in rats. Toxins, 11, (11) 642.

 Aslanturk, O. S. and T. A. Celik. 2013. Antioxidant, cytotoxic and apoptotic activities of extracts from medicinal plant Euphorbia platyphyllos L. J Med Plants Res, 7, (19) 1293-1304.

Cavalcante, N. B., A. D. Da Conceição Santos and J. R. G. Da Silva Almeida. 2020. The genus Jatropha (Euphorbiaceae): A review on secondary chemical metabolites and biological aspects. Chemico-biological interactions, 318, 108976.

  De Souza, L. S., L. C. Puziol, C. L. Tosta, M. L. Bittencourt, J. Santa Ardisson, R. R. Kitagawa, P. R. Filgueiras and R. M. Kuster. 2019. Analytical methods to access the chemical composition of an Euphorbia tirucalli anticancer latex from traditional Brazilian medicine. Journal of ethnopharmacology, 237, 255-265.

Ekta, S., S. Sheel, D. Jaya and S. Swapnil. 2012. Diversified potentials of Ocimum sanctum Linn (tulsi): an exhaustive survey. Journal of Natural Product and Plant Resources, 2, (1) 39-48.

 Ernst, M., O. M. Grace, C. H. Saslis-Lagoudakis, N. Nilsson, H. T. Simonsen and N. Rønsted. 2015. Global medicinal uses of Euphorbia L.(Euphorbiaceae). Journal of ethnopharmacology, 176, 90-101.

Ertas, A., M. A. Yilmaz and M. Firat. 2015. Chemical profile by LC–MS/MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two Euphorbia species. Natural product research, 29, (6) 529-534.

Fais, A., G. L. Delogu, S. Floris, B. Era, R. Medda and F. Pintus. 2021. Euphorbia characias: Phytochemistry and biological activities. Plants, 10, (7) 1468.

Fankhauser, C. and J. M. Christie. 2015. Plant phototropic growth. Current Biology, 25, (9) R384-R389.

Gallavotti, A. 2013. The role of auxin in shaping shoot architecture. Journal of experimental botany, 64, (9) 2593-2608.

Ghorai, N., S. Chakraborty, S. Gucchait, S. K. Saha and S. Biswas. 2012. Estimation of total Terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent.

Gopi, K., K. Renu, B. S. Vishwanath and G. Jayaraman. 2015. Protective effect of Euphorbia hirta and its components against snake venom induced lethality. Journal of ethnopharmacology, 165, 180-190.

Hosseini Hashemi, S. , Kh., Bagheri, and A.V.R. Safdari, and H. Sadeghifar,2011. The chemical composition of the ethanol extract of black locust  wood by using gas chromatography - mass spectrometry. Journal of Sciences and Techniques in Natural Resources, Vol 6 (3): 63-74.  (In Persian)

Jokar, E., A. Sateei, M. Ebadi and M. Ahmadi Golsefidi. 2022. Comparative study of the effect of some types of nitrogen fertilizers on growth, alkaloid content and some physiological traits of the ornamental-medicinal plant Agave americana cv marginata under greenhouse cultivation. Journal of Iranian Plant Ecophysiological Research, 16, (64) 111-126.

Kaur, N., B. Kaur and G. Sirhindi. 2017. Phytochemistry and pharmacology of Phyllanthus niruri L.: a review. Phytotherapy research, 31, (7) 980-1004.

Kebede, T., E. Gadisa and A. Tufa. 2021. Antimicrobial activities evaluation and phytochemical screening of some selected medicinal plants: A possible alternative in the treatment of multidrug-resistant microbes. PLoS One, 16, (3) e0249253.

Lazari, D. M., H. D. Skaltsa and T. Constantinidis. 2000. Composition of the essential oil of Herniaria incana Lam. from Greece. Journal of Essential Oil Research, 12, (4) 435-437.

Lee, K. H., J. H. Kim, D. S. Lim and C. H. Kim. 2000. Anti-leukaemic and anti-mutagenic effects of di (2-ethylhexyl) phthalate isolated from Aloe vera Linne. Journal of Pharmacy and Pharmacology, 52, (5) 593-598.

 Lourith, N. and M. Kanlayavattanakul. 2013. Hair loss and herbs for treatment. Journal of cosmetic dermatology, 12, (3) 210-222.

 Meng, J., B. T. Li, G. Sheng, A. L. Zhang, X. C. Li and J. M. Tian. 2021. Cytotoxic diterpenoids from Euphorbia fischeriana. Chemistry & Biodiversity, 18, (2) e2000919.

 Morteza-Semnani, K., M. Saeedi and M. Akbarzadeh. 2016. Chemical composition of the essential oil of the flowering aerial parts of Lamium album L. Journal of Essential Oil Bearing Plants, 19, (3) 773-777.

 Murakami, Y., C. Murakami, F. Hoshino, Q. Lu, R. Akiyama, A. Yamaki, D. Takahashi and F. Sakane. 2020. Palmitic acid-and/or palmitoleic acid-containing phosphatidic acids are generated by diacylglycerol kinase α in starved Jurkat T cells. Biochemical and Biophysical Research Communications, 525, (4) 1054-1060.

 Muraro, D., H. Byrne, J. King, U. Voß, J. Kieber and M. Bennett. 2011. The influence of cytokinin–auxin cross-regulation on cell-fate determination in Arabidopsis thaliana root development. Journal of theoretical biology, 283, (1) 152-167.

 Mwine, J., P. Van Damme, B. R. Hastilestari and J. Papenbrock. 2013. Euphorbia tirucalli L.(Euphorbiaceae)–the miracle tree: current status of knowledge. African Natural Plant Products Volume II: Discoveries and Challenges in Chemistry, Health, and Nutrition, 3-17.

 Rahmani, A., A. Sateei, M. Ebadi and M. Ahmadi Golsefidi. 2021. Investigation of growth, nitrate reductase activity, total content of flavonoids, anthocyanins and some elements in Zamioculcas zamiifolia Engl. under the influence of three types of nitrogen fertilizers in greenhouse conditions. Journal of Iranian Plant Ecophysiological Research,

Rui, Z., W. Xiu-Juan and G. Wei. 2020. Regulation mechanism of plant hormones on secondary metabolites. Zhongguo Zhong yao za zhi= Zhongguo Zhongyao Zazhi= China Journal of Chinese Materia Medica, 45, (17) 4205-4210.

 Saad, B. and O. Said. 2011. Greco-Arab and Islamic herbal medicine: traditional system, ethics, safety, efficacy, and regulatory issues. John Wiley & Sons

 Salehi, B., A. Prakash Mishra, M. Nigam, N. Karazhan, I. Shukla, A. Kiełtyka‐Dadasiewicz, B. Sawicka, A. Głowacka, M. S. Abu‐Darwish and A. Hussein Tarawneh. 2021. Ficus plants: state of the art from a phytochemical, pharmacological, and toxicological perspective. Phytotherapy Research, 35, (3) 1187-1217.

 Sampson, J. and D. De Korte. 2011. DEHP‐plasticised PVC: relevance to blood services. Transfusion medicine, 21, (2) 73-83.

Soleha, M., D. Pratiwi, I. Sari, E. Hermiyanti, N. Yunarto and H. Setyorini.2020. Antioxidant Activity of Methanol Extract Tetracera scanden L. Merr Predicted Active Compound of Methanol Extract with GCMS NIST Library. Proc. Journal of Physics: Conference Series, 1665:012028: IOP Publishing.

Swamy, M. K., G. Arumugam, R. Kaur, A. Ghasemzadeh, M. M. Yusoff and U. R. Sinniah. 2017. GC-MS based metabolite profiling, antioxidant and antimicrobial properties of different solvent extracts of Malaysian Plectranthus amboinicus leaves. Evidence-Based Complementary and Alternative Medicine, 2017,

Tajik, M., R. Vaysi and M. Kiaei. 2014. Extraction, Identification and comparison of organically chemical components in extractive of bark and wood from Eucalyptus by Gas chromatography-Mass spectrometry. Iranian Journal of Wood and Paper Science Research, 29, (WINTER 4) 644-652.

Vaishnav, P. and A. L. Demain. 2011. Unexpected applications of secondary metabolites. Biotechnology Advances, 29, (2) 223-229.

Yang, Y., X. Chen, F. Luan, M. Wang, Z. Wang, J. Wang and X. He. 2021. Euphorbia helioscopia L.: A phytochemical and pharmacological overview. Phytochemistry, 184, 112649.