Salinity stress alleviation by use of silicon in Ocimum basilicum L.: an approach based on enhancing antioxidant responses
الموضوعات :Shahla Sharifian Jazi 1 , Latifeh Pourakbar 2 , Shokofe Enteshari 3
1 - Department of biology, faculty of Science, Urmia University
2 - Department of Biology, faculty of Science, Urmia university, Urmia, Iran.
3 - Department of Chemistry, Payame Noor University, PO Box 3697-19395, Tehran, Iran
الکلمات المفتاحية: basil, SOD activity, H2O2 content, CAT activity, sodium chloride stress,
ملخص المقالة :
Basil (Ocimum basilicum L.) is one of the valuable medicinal plants. Negative effects of NaCl stress on plants have been reported and silicon may alleviate these negative effects through promoting antioxidant system. This study was conducted to investigate the effects of silicon as elicitor and NaCl as salinity stress on some morphological, biochemical, and antioxidant parameters in basil plant. The plants were pretreated with silicon (0, 0.5, and 1.50 mM) and submitted to NaCl stress (0, 50 and 100 mM). Results showed that NaCl stress decreased dry and fresh weight of shoot and root, cholorophyll and carbohydrate content, but carotenoid, hydrogen peroxide (H2O2) content, superoxide dismutase (SOD) and catalase (CAT) activity increased (p<0.05). Silicon also increased dry and fresh weight of shoot and root, carotenoid, chlorophyll and carbohydrate content, CAT and SOD activity but H2O2 content decreased (p<0.05). Based on these results, silicon especially 1.50 mM concentration is recommended for protection of basil under NaCl stress.
Abbasi, H., M. Jamil, A. Haq, S. Ali, R. Ahmad, Z. Malik and Z. Parveen. 2016. Salt stress manifestation on plants, mechanism of salt tolerance and potassium role in alleviating it: a review. Zemdirbyste-Agriculture, 103, (2) 229-238.
Abdel-Latif, A. and F. El-Demerdash. 2017. The ameliorative effects of silicon on salt-stressed sorghum seedlings and its influence on the activities of sucrose synthase and PEP carboxylase. J Plant Physiol Pathol 5, 2, 2.
Acosta-Motos, J. R., M. F. Ortuño, A. Bernal-Vicente, P. Diaz-Vivancos, M. J. Sanchez-Blanco and J. A. Hernandez. 2017. Plant responses to salt stress: adaptive mechanisms. Agronomy, 7, (1) 18.
Alves, R. D. C., M. C. M. Nicolau, M. V. Checchio, G. D. S. Sousa, F. D. a. D. Oliveira, R. M. Prado and P. L. Gratão. 2020. Salt stress alleviation by seed priming with silicon in lettuce seedlings: an approach based on enhancing antioxidant responses. Bragantia, 79, 19-29.
Avestan, S., M. Ghasemnezhad, M. Esfahani and C. S. Byrt. 2019. Application of nano-silicon dioxide improves salt stress tolerance in strawberry plants. Agronomy, 9, (5) 246.
Daoud, A., M. Hemada and A. El-Araby. 2018. Effect of silicon on the tolerance of wheat (Triticum aestivum L.) to salt stress at different growth stages: case study for the management of irrigation water. Plants, 7, (2) 29.
Delavar, K., F. Ghanati, H. Zare-Maivan and M. Behmanesh. 2016. The effect of the silicon and aluminum interaction on the physiological parameters of maize. Iranian Journal of Plant Physiology, 6, (4) 1785-1794.
Fales, F. W. 1951. The assimilation and degradation of carbohydrates by yeast cells. Journal of Biological Chemistry, 193, (1) 113-124.
Farzamisepehr, M., M. Ghorbanli and Z. Tadji. 2021. Effect of Drought Stress on Some Growth Parameters and Several Biochemical Aspects in Two Pumpkin Species. Iranian Journal of Plant Physiology, 11, (3) 3731-3740.
Giannopolitis, C. N. and S. K. Ries. 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant physiology, 59, (2) 309-314.
Gomes, M. a. D. C., I. A. Pestana, C. Santa-Catarina, R. A. Hauser-Davis and M. S. Suzuki. 2017. Salinity effects on photosynthetic pigments, proline, biomass and nitric oxide in Salvinia auriculata Aubl. Acta Limnologica Brasiliensia, 29, .
Guo, W.-Q., P.-T. Zhang, C.-H. Li, J.-M. Yin and X.-Y. Han. 2015. Recovery of root growth and physiological characters in cotton after salt stress relief. Chilean journal of agricultural research, 75, (1) 85-91.
Hajiboland, R., L. Cherghvareh and F. Dashtebani. 2017. Effect of silicon supplementation on wheat plants under salt stress. Journal of Plant Process and Function, 5, (18).
Hasanuzzaman, M., M. Bhuyan, K. Nahar, M. Hossain, J. A. Mahmud, M. Hossen, A. a. C. Masud and M. Fujita. 2018. Potassium: a vital regulator of plant responses and tolerance to abiotic stresses. Agronomy, 8, (3) 31.
Heidarian, F. and P. Roshandel. 2020. Enhancement of salt tolerance in black bean variety (Phaseolus vulgaris L.) by silicon nutrition. Iranian Journal of Plant Physiology, 10, (3) 3255-3264.
Hewitt, E. J. 1966. Sand and water culture methods used in the study of plant nutrition. Farnham Royal : Commonwealth Agricultural Bureaux,
Hoffmann, J., R. Berni, J.-F. Hausman and G. Guerriero. 2020. A review on the beneficial role of silicon against salinity in non-accumulator crops: tomato as a model. Biomolecules, 10, (9) 1284.
Isayenkov, S. V. and F. J. Maathuis. 2019. Plant salinity stress: many unanswered questions remain. Frontiers in Plant Science, 10, 80.
Jahan, M., S. Ghalenoee, A. Khamooshi and M. Amiri. 2015. Evaluation of Some Agroecological Characteristics of Basil (Ocimum basilicum L.) as Affected by Simultaneous Application of Water-Saving Superabsorbent Hydrogel in Soil and Foliar Application of Humic Acid under Different Irrigation Intervals in a Low Inp. Journal Of Horticultural Science, 29, (2) 240-254.
Kar, M. and D. Mishra. 1976. Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant physiology, 57, (2) 315-319.
Khan, A., A. L. Khan, S. Muneer, Y.-H. Kim, A. Al-Rawahi and A. Al-Harrasi. 2019. Silicon and salinity: crosstalk in crop-mediated stress tolerance mechanisms. Frontiers in plant science, 10, 1429.
Khorasaninejad, S., F. Zare and K. Hemmati. 2020. Effects of silicon on some phytochemical traits of purple coneflower (Echinacea purpurea L.) under salinity. Scientia Horticulturae, 264, 108954.
Luyckx, M., J.-F. Hausman, S. Lutts and G. Guerriero. 2017. Impact of silicon in plant biomass production: focus on bast fibres, hypotheses, and perspectives. Plants, 6, (3) 37.
Mauad, M., C. a. C. Crusciol, A. S. Nascente, H. Grassi and G. P. P. Lima. 2016. Effects of silicon and drought stress on biochemical characteristics of leaves of upland rice cultivars. Revista Ciência Agronômica, 47, 532-539.
Moussa, H. and M. Galad. 2015. Comparative response of salt tolerant and salt sensitive maize (Zea mays L.) cultivars to silicon. European Journal of Academic Essays, 2, (1) 1-5.
Muscolo, A., A. Junker, C. Klukas, K. Weigelt-Fischer, D. Riewe and T. Altmann. 2015. Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions. Journal of experimental botany, 66, (18) 5467-5480.
Pervaiz, S., H. Gul, M. Rauf, H. I. Mohamed, K. Ur Rehman, H. Wasila, I. Ahmad, S. T. Shah, A. Basit and M. Ahmad. 2023. Screening of Linum usitatissimum Lines Using Growth Attributes, Biochemical Parameters and Ionomics Under Salinity Stress. Gesunde Pflanzen, 1-19.
Porra, R., W. Thompson and P. Kriedemann. 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 975, (3) 384-394.
Ragel De La Torre, P., N. Raddatz, E. O. Leidi Montes, F. J. Quintero and J. M. Pardo. 2019. Regulation of K+ Nutrition in Plants. Frontiers in Plant Science, 10 (281), 1-21,
Rezende, R. a. L. S., F. A. Rodrigues, J. D. R. Soares, H. R. D. O. Silveira, M. Pasqual and G. D. M. G. Dias. 2017. Salt stress and exogenous silicon influence physiological and anatomical features of in vitro-grown cape gooseberry. Ciência Rural, 48,
Rios-Lozano, A., H. Ramos-Sotelo, C. Reyes-Moreno and M. G. Figueroa-Pérez. 2023. Enhancement of nutraceutical properties of goldenberry (Physalis peruviana L.) leaves through foliar application of salicylic acid during cultivation. Food Science and Technology Research, FSTR-D-23-00106.
Rios, J. J., M. C. Martínez-Ballesta, J. M. Ruiz, B. Blasco and M. Carvajal. 2017. Silicon-mediated improvement in plant salinity tolerance: the role of aquaporins. Frontiers in plant science, 8, 948.
Sagisaka, S. 1976. The occurrence of peroxide in a perennial plant, Populus gelrica. Plant Physiology, 57, (2) 308-309.
Sattar, A., M. A. Cheema, A. Sher, M. Ijaz, S. Ul-Allah, A. Nawaz, T. Abbas and Q. Ali. 2019. Physiological and biochemical attributes of bread wheat (Triticum aestivum L.) seedlings are influenced by foliar application of silicon and selenium under water deficit. Acta Physiologiae Plantarum, 41, (8) 1-11.
Shanan, N. and Z. El Sadek. 2017. Influence of silicon on tuberose plants under drought conditions. Middle East Journal of Agriculture Research, 6, (02) 348-360.
Soundararajan, P., A. Manivannan, C. H. Ko, S. Muneer and B. R. Jeong. 2017. Leaf physiological and proteomic analysis to elucidate silicon induced adaptive response under salt stress in Rosa hybrida ‘Rock Fire’. International journal of molecular sciences, 18, (8) 1768.
Torabi, F., A. Majd and S. Enteshari. 2015. The effect of silicon on alleviation of salt stress in borage (Borago officinalis L.). Soil science and plant nutrition, 61, (5) 788-798.
Tubana, B. S., T. Babu and L. E. Datnoff. 2016. A review of silicon in soils and plants and its role in US agriculture: history and future perspectives. Soil Science, 181, (9/10) 393-411.
Wang, S., P. Liu, D. Chen, L. Yin, H. Li and X. Deng. 2015. Silicon enhanced salt tolerance by improving the root water uptake and decreasing the ion toxicity in cucumber. Frontiers in plant science, 6, 759.
Yarsi, G., O. Altuntas, A. Sivaci and H. Y. Dasgan. 2017. Effects of salinity stress on plant growth and mineral composition of grafted and ungrafted galia C8 melon cultivar. Pak J Bot, 49, (3) 819-822.
Yavaş, İ. and Ü. Aydın. 2017. The role of silicon under biotic and abiotic stress conditions. Türkiye Tarımsal Araştırmalar Dergisi, 4, (2) 204-209.
Yazdani, M., S. Enteshari, S. Saadatmand and S. Habibollahi. 2021. Effects of silicon on glycine-betaine, phytochelatin, and antioxidant enzymes in licorice (Glycyrrhiza glabra L.) under aluminum stress. Iranian Journal of Plant Physiology, 11, (2) 3625-3635.
Zhang, W., Z. Xie, L. Wang, M. Li, D. Lang and X. Zhang. 2017. Silicon alleviates salt and drought stress of Glycyrrhiza uralensis seedling by altering antioxidant metabolism and osmotic adjustment. Journal of plant research, 130, (3) 611-624.
Zhu, Y.-X., H.-J. Gong and J.-L. Yin. 2019. Role of silicon in mediating salt tolerance in plants: a review. Plants, 8, (6) 147.
Zhu, Y., X. Jiang, J. Zhang, Y. He, X. Zhu, X. Zhou, H. Gong, J. Yin and Y. Liu. 2020. Silicon confers cucumber resistance to salinity stress through regulation of proline and cytokinins. Plant Physiology and Biochemistry, 156, 209-220.
