Influence of ascorbic acid on growth and micropropagation of Aloe barbadensis Mill.
الموضوعات :
Aki Ramezani Sayad
1
,
Ahmad Majd
2
,
Mojtaba Khorrami Raad
3
,
Behzad Kaviani
4
1 - Department of Tissue Culture, North Region Branch of Iran (Rasht), Agricultural Biotechnology Research Institute of Iran (ABRII)
2 - Department of Biology, Faculty of Sciences, Tarbiat-e-Moallem University, Tehran, Iran
3 - School of Ecosystem and Forest Sciences, Faculty of Science, The University of Melbourne, Creswick, Australia
4 - Department of Horticultural Science, Faculty of Agricultural Science, Islamic Azad University, Rasht, Iran
الکلمات المفتاحية: Medicinal Plants, tissue culture, plant growth regulators, Vegetative growth, Aloe,
ملخص المقالة :
Ascorbic acid (AsA) plays various roles in plant metabolism. This study was carried out to evaluate the effect of different concentrations of AsA on the growth and micropropagation of Aloe barbadensis Mill., an important medicinal and ornamental herb, for the first time. In this regard, results obtained from applying different concentrations of AsA on variables such as aerial part length, root length and number, number of propagules, medium browning, and the fresh and dry weights of plants were analysed after 8 weeks. Control plants showed slower growth in the aerial parts compared to plants treated with AsA. Additionally, the leaves were smaller in the control plants. The fresh and dry weights of the aerial parts were less in the control plants than in those treated with AsA. There was a significant increase in the number of propagules produced in the different treatments compared to the control treatment. The average number and length of roots produced in plants treated with AsA were higher than those produced in the control plants. Furthermore, browning of the medium and tissue cultures was reduced in plants treated with AsA due to the presence of different phenolic compounds in these plants. Overall, AsA at a concentration of 80 mg L-1 had the greatest effect on the induction of growth and development of A. barbadensis Mill. in vitro.
Chen, Z., X.-L. Cao and J.-P. Niu. 2021. Effects of exogenous ascorbic acid on seed germination and seedling salt-tolerance of alfalfa. PLoS One, 16, (4) e0250926.
Córdoba, F. and J. A. González-Reyes. 1994. Ascorbate and plant cell growth. Journal of bioenergetics and biomembranes, 26, 399-405.
Del Carmen Córdoba-Pedregosa, M., F. Córdoba, J. M. Villalba and J. A. González-Reyes. 2003. Zonal changes in ascorbate and hydrogen peroxide contents, peroxidase, and ascorbate-related enzyme activities in onion roots. Plant Physiology, 131, (2) 697-706.
Duyff, R. L. 2017. Academy of Nutrition and Dietetics complete food and nutrition guide. (No Title),
Habibie, A., N. Yazdani, M. K. Saba and K. Vahdati. 2019. Ascorbic acid incorporated with walnut green husk extract for preserving the postharvest quality of cold storage fresh walnut kernels. Scientia Horticulturae, 245, 193-199.
Hashemabadi, D. and B. Kaviani. 2008. Rapid micro-propagation of Aloe vera L. via shoot multiplication. African Journal of Biotechnology, 7, (12)
Horemans, N., C. H. Foyer and H. Asard. 2000. Transport and action of ascorbate at the plant plasma membrane. Trends in plant science, 5, (6) 263-267.
Kaviani, B. 2014. Micropropagation of Matthiola incana using BA and IBA. Iranian Journal of Plant Physiology, 4, (3) 1071-1078.
Kaviani, B., S. Sedaghathoor, M. R. Safari Motlagh and S. Rouhi. 2019. Influence of plant growth regulators (BA, TDZ, 2-iP and NAA) on micropropagation of Aglaonema widuri. Iranian Journal of Plant Physiology, 9, (4) 2901-2909.
Kka, N., J. Rookes and D. Cahill. 2018. The influence of ascorbic acid on root growth and the root apical meristem in Arabidopsis thaliana. Plant physiology and biochemistry, 129, 323-330.
Lo’ay, A. and A. El-Khateeb. 2018. Antioxidant enzyme activities and exogenous ascorbic acid treatment of ‘Williams’ banana during long-term cold storage stress. Scientia Horticulturae, 234, 210-219.
Martínez-Ortiz, M. A., H. M. Palma-Rodríguez, E. Montalvo-González, S. G. Sáyago-Ayerdi, R. Utrilla-Coello and A. Vargas-Torres. 2019. Effect of using microencapsulated ascorbic acid in coatings based on resistant starch chayotextle on the quality of guava fruit. Scientia Horticulturae, 256, 108604.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum, 15, (3)
Niguse, M., D. B. Sbhatu and H. B. Abraha. 2020. In vitro micropropagation of Aloe adigratana Reynolds using offshoot cuttings. The Scientific World Journal, 2020, (1) 9645316.
Potters, G., N. Horemans, R. J. Caubergs and H. Asard. 2000. Ascorbate and dehydroascorbate influence cell cycle progression in a tobacco cell suspension. Plant Physiology, 124, (1) 17-20.
Shibru, S., G. Olani and A. Debebe. 2018. In vitro propagation of Aloe vera Linn from shoot tip culture. GSC Biological and Pharmaceutical Sciences, 4, (2) 01-06.
Singh, M. K., T. Yadav and R. K. Raman. 2020. A quick method for micro-propagation of Aloe vera L. from leaf explants via callus induction. Journal of Entomology and Zoology Studies, 8, (2) 201-206.
Taha, L. S., S. S. Sayed, M. Farahat and I. M. El-Sayed. 2018. In vitro culture and bulblets induction of Asiatic hybrid lily'red alert'. Journal of Biological Sciences,84-91.
Uikey, S., M. Tripathi, G. Tiwari, A. Pandey and R. Patel. 2013. Microcloning studies in Aloe barbadensis. Plant Cell Biotechnol Mol Biol, 14, 1-11.
Welehaweria, M. and D. B. Sbhatu. 2023. In vitro micropropagation of Aloe elegans Tod. using offshoot cuttings. BMC Research Notes, 16, (1) 215.
Youssef, N. M., S. A. Shaaban, Z. F. Ghareeb and L. S. Taha. 2019. In vitro bulb formation of direct and indirect regeneration of Lilium orientalis cv.“Starfighter” plants. Bulletin of the National Research Centre, 43, 1-9.
