Nitric oxide ameliorates salinity tolerance in Pyrodwarf pear (Pyrus communis) rootstocks by regulating polyamine content
الموضوعات :
Mehri Yousefi
1
,
Lotfali Naseri
2
,
Fariborz Zaare-Nahandi
3
1 - Department of Horticultural Science, Urmia University, Urmia, Iran
2 - Department of Horticultural Science, Urmia University, Urmia, Iran
3 - Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
الکلمات المفتاحية: NaCl, nitric oxide, Rootstock, Polyamines, pear,
ملخص المقالة :
Nitric oxide (NO), an endogenous signaling molecule, is involved in various physiological processes and stress responses in plants. In the present research, Pyrodwarf pear (Pyrus communis) rootstocks were grown by nutrient solution to investigate the effects of sodium nitroprusside (SNP) application as an NO donor at 0, 0.1, 0.5, and 1 mM levels on plant stress tolerance, content of main polyamines, physiological reactions, and activity of CAT and APX enzymes under NaCl stress condition at 0, 50, 100, and 150 mM concentrations. Exogenous NO significantly increased endogenous NO content of leaves, height of the plants and relative water content (RWC) of leaves while it decreased malondialdehyde (MDA) content. NaCl stress increased the content of putrescine (Put) and spermine (Spm), and exogenous NO resulted in a further increase the content of Spm in plant leaves under NaCl stress. Pyrodwarf pear rootstocks significantly showed higher content of proline and antioxidant activity under NaCl stress, and NO treatment further increased the content of proline and antioxidant activity in plants exposed to NaCl stress. The activity of antioxidant enzymes including catalase (CAT) and peroxidase (POD) increased in plants under NaCl stress, and exogenous NO further induced those antioxidant enzyme activities. Exogenous NO resulted in the enhancement of Spm content and plants with higher Spm content exhibited high levels of antioxidant activity and proline content under NaCl stress, indicating the beneficial effects of exogenous NO under salinity conditions. NO improved the tolerance of Pyrodwarf pear rootstocks under NaCl stress by regulating the content and the ratio of polyamines.
Aebi, H., 1984. [13] 'Catalase in vitro', Methods in enzymology. Elsevier, pp: 121-126.
Ahmad, P., A.A. Abdel Latef, A. Hashem, E.F. Abd_Allah, S. Gucel and L.S.P. Tran. 2016. 'Nitric oxide mitigates salt stress by regulating levels of osmolytes and antioxidant enzymes in chickpea'. Frontiers in plant science, 7: 347.
Alcázar, R., T. Altabella, F. Marco, C. Bortolotti, M. Reymond, C. Koncz, P. Carrasco and A.F.Tiburcio. 2010. 'Polyamines: molecules with regulatory functions in plant abiotic stress tolerance'. Planta, 231: 1237-1249.
Ashraf, M. and M. Foolad, 2007. 'Roles of glycine betaine and proline in improving plant abiotic stress resistance'. Environmental and experimental botany, 59: 206-216.
Bachrach, U. 2010. 'The early history of polyamine research'. Plant Physiology and Biochemistry, 48: 490-495.
Bates, L., R. Waldren and I. Teare. 1973. 'Rapid determination of free proline for water-stress studies'. Plant and soil, 39: 205-207.
Brand-Williams, W., M. E. Cuvelier and C. Berset. 1995. 'Use of a free radical method to evaluate antioxidant activity'. LWT-Food science and Technology, 28: 25-30.
Chance, B. and A. Maehly. 1955. 'Assay of catalases and peroxidases'. Methods in Enzymology, 2, 764-775.
Corpas, F.J., A. Carreras, R. Valderrama, M. Chaki, J.M. Palma, L. A. del Río and J. B. Barroso. 2007. 'Reactive nitrogen species and nitrosamine stress in plants'. Plant Stress,1: 37-41.
Dong, Y., S. Jinc, S. Liu, L. Xu and J. Kong. 2014. 'Effects of exogenous nitric oxide on growth of cotton seedlings under NaCl stress'. Journal of soil science and plant nutrition, 14: 1-13.
Duan, J., J. Li, S. Guo and Y. Kang. 2008. 'Exogenous spermidine affects polyamine metabolism in salinity-stressed Cucumis sativus roots and enhances short-term salinity tolerance'. Journal of plant physiology, 165: 1620-1635.
Fan, H.-F., C.-X. Du and S.-R. Guo. 2013. 'Nitric oxide enhances salt tolerance in cucumber seedlings by regulating free polyamine content'. Environmental and Experimental Botany, 86: 52-59.
Fan, H., S. Guo, Y. Jiao, R. Zhang and J. Li. 2007. 'Effects of exogenous nitric oxide on growth, active oxygen species metabolism, and photosynthetic characteristics in cucumber seedlings under NaCl stress '. Frontiers of Agriculture in China, 1: 308-314.
Fan, Q.-J. and j.H. Liu. 2012. 'Nitric oxide is involved in dehydration/drought tolerance in Poncirus trifoliata seedlings through regulation of antioxidant systems and stomatal response '. Plant cell reports, 31: 145-154.
Filippou, P., C. Antoniou and V. Fotopoulos. 2013. 'The nitric oxide donor sodium nitroprusside regulates polyamine and proline metabolism in leaves of Medicago truncatula plants'. Free Radical Biology and Medicine, 56: 172-183.
Flores, H.E. and A.W. Galston. 1982. 'Analysis of polyamines in higher plants by high performance liquid chromatography'. Plant Physiology, 69: 701-706.
Garufi, A., S. Visconti, L. Camoni and P. Aducci. 2007. 'Polyamines as physiological regulators of 14-3-3 interaction with the plant plasma membrane H+-ATPase'. Plant and cell physiology, 48: 434-440.
Green, L.C., D.A. Wagner, J. Glogowski, P.L. Skipper, J.S. Wishnok and S.R. Tannenbaum. 1982. 'Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids'. Analytical biochemistry, 126: 131-138.
Groppa, M. and M. Benavides. 2008. 'Polyamines and abiotic stress: recent advances'. Amino acids, 34(1): 35-45.
Hasanuzzaman, M., M.A. Hossain and M. Fujita. 2011. 'Nitric oxide modulates antioxidant defense and the methylglyoxal detoxification system and reduces salinity-induced damage of wheat seedlings'. Plant Biotechnology Reports, 5: 353-365.
Hayat, S., S.A. Hasan, M. Mori, Q. Fariduddin and A. Ahmad. 2010. 'Nitric oxide: chemistry, biosynthesis, and physiological role'. Nitric oxide in plant physiology, 1-15.
Hayat, S., S. Yadav, A.S. Wani, M. Irfan, M.N. Alyemini and A. Ahmad. 2012. 'Impact of sodium nitroprusside on nitrate reductase, proline content, and antioxidant system in tomato under salinity stress'. Horticulture, Environment, and Biotechnology, 53: 362-367.
He, L., Y. Ban, H. Inoue, N. Matsuda, J. Liu and T. Moriguchi. 2008. 'Enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis'. Phytochemistry, 69: 2133-2141.
Kotakis, C., E. Theodoropoulou, K. Tassis, C. Oustamanolakis, N.E. Ioannidis and K. Kotzabasis. 2014. 'Putrescine, a fast-acting switch for tolerance against osmotic stress'. Journal of plant physiology, 171: 48-51.
Krishnamurthy, R. and K. Bhagwat. 1989. 'Polyamines as modulators of salt tolerance in rice cultivars'. Plant Physiology, 91: 500-504.
Lamattina, L., C. García-Mata, M. Graziano and G. Pagnussat. 2003. 'Nitric oxide: the versatility of an extensive signal molecule'. Annual review of plant biology, 54: 109-136.
Leshem, Y. and E. Haramaty. 1996. 'Plant aging: the emission of NO and ethylene and effect of NO-releasing compounds on growth of pea (Pisum sativum) foliage'. J Plant Physiol, 148: 258-263.
Lin, Y., Z. Liu, Q. Shi, X. Wang, M. Wei, and F. Yang. 2012. 'Exogenous nitric oxide (NO) increased antioxidant capacity of cucumber hypocotyl and radicle under salt stress'. Scientia horticulturae, 142: 118-127.
Liu, J.-H., K. Nada, C. Honda, H. Kitashiba, X.-P. Wen, X.-M. Pang and T. Moriguchi. 2006. 'Polyamine biosynthesis of apple callus under salt stress: importance of the arginine decarboxylase pathway in stress response'. Journal of Experimental Botany, 57: 2589-2599.
Maas, E., 1993. 'Salinity and citriculture'. Tree physiology, 12(2): 195-216.
Manai, J., T. Kalai, H. Gouia and F. Corpas. 2014. 'Exogenous nitric oxide (NO) ameliorates salinity-induced oxidative stress in tomato (Solanum lycopersicum) plants'. Journal of soil science and plant nutrition, 14: 433-446.
Mostofa, M.G., M. Fujita and L.-S.P. Tran. 2015. 'Nitric oxide mediates hydrogen peroxide-and salicylic acid-induced salt tolerance in rice (Oryza sativa L.) seedlings'. Plant growth regulation, 77: 265-277.
Musacchi, S., M. Quartieri and M. Tagliavini. 2006. 'Pear (Pyrus communis) and quince (Cydonia oblonga) roots exhibit different ability to prevent sodium and chloride uptake when irrigated with saline water'. European journal of agronomy, 24: 268-275.
Okubo, M. and T. Sakuratani. 2000. 'Effects of sodium chloride on survival and stem elongation of two Asian pear rootstock seedlings'. Scientia Horticulturae, 85: 85-90.
Reddy, P.S., G. Jogeswar, G.K. Rasineni, M. Maheswari, A.R. Reddy, R.K. Varshney and P.K. Kishor. 2015. 'Proline over-accumulation alleviates salt stress and protects photosynthetic and antioxidant enzyme activities in transgenic sorghum [Sorghum bicolor (L.) Moench]'. Plant Physiology and Biochemistry, 94: 104-113.
Repellin, A., A.P. Thi, A. Tashakorie, Y. Sahsah, C. Daniel and Y. Zuily-Fodil. 1997. 'Leaf membrane lipids and drought tolerance in young coconut palms (Cocos nucifera L.)'. European Journal of Agronomy, 6: 25-33.
Rhoades, J.D. 1992. 'The use of saline waters for crop production'. FAO.
Ruan, H.-H., W.B. Shen and L.L. Xu. 2004. 'Nitric oxide involved in the abscisic acid induced proline accumulation in wheat seedling leaves under salt stress'. ACTA BOTANICA SINICA-ENGLISH EDITION, 46: 1307-1315.
Shan, C., Y. Zhou and M. Liu. 2015. 'Nitric oxide participates in the regulation of the ascorbate-glutathione cycle by exogenous jasmonic acid in the leaves of wheat seedlings under drought stress'. Protoplasma, 252: 1397-1405.
Shen, W., K. Nada and S. Tachibana. 2000. 'Involvement of polyamines in the chilling tolerance of cucumber cultivars'. Plant Physiology, 124: 431-440.
Shi, Q., F. Ding, X. Wang and M. Wei. 2007. 'Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress'. Plant Physiology and Biochemistry, 45: 542-550.
Tanou, G., P. Filippou, M. Belghazi, D. Job, G. Diamantidis, V. Fotopoulos and A. Molassiotis. 2012. 'Oxidative and nitrosative-based signaling and associated post-translational modifications orchestrate the acclimation of citrus plants to salinity stress'. The Plant Journal, 72: 585-599.
Tanou, G., A. Molassiotis and G. Diamantidis. 2009. 'Hydrogen peroxide-and nitric oxide-induced systemic antioxidant prime-like activity under NaCl-stress and stress-free conditions in citrus plants'. Journal of plant physiology, 166: 1904-1913.
Tuteja, N. and S.K. Sopory. 2008. 'Chemical signaling under abiotic stress environment in plants'. Plant signaling & behavior, 3: 525-536.
Uchida, A., A.T. Jagendorf, T. Hibino and T. Takabe. 2002. 'Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice'. Plant Science, 163: 515-523.
|
|
Urano, K., K. Maruyama, Y. Ogata, Y. Morishita, M. Takeda, N. Sakurai, H. Suzuki, K. Saito, D. Shibata and M. Kobayashi. 2009. 'Characterization of the ABA-regulated global responses to dehydration in Arabidopsis by metabolomics'. The Plant Journal, 57: 1065-1078.
Wen, X.-P., Y. Ban, H. Inoue, N. Matsuda, M. Kita and T. Moriguchi. 2011. 'Antisense inhibition of a spermidine synthase gene highlights the role of polyamines for stress alleviation in pear shoots subjected to salinity and cadmium'. Environmental and Experimental Botany, 72: 157-166.
Wu, X., W. Zhu, H. Zhang, H. Ding and H.J. Zhang. 2011. 'Exogenous nitric oxide protects against salt-induced oxidative stress in the leaves from two genotypes of tomato (Lycopersicom esculentum Mill.)'. Acta physiologiae plantarum, 33: 1199-1209.
Xu, Y., X. Sun, J. Jin and H. Zhou. 2010. 'Protective effect of nitric oxide on light-induced oxidative damage in leaves of tall fescue'. Journal of plant physiology, 167: 512-518.
Zeng, C.-L., L. Liu, B. R. Wang, X.M. Wu and Y. Zhou. 2011. 'Physiological effects of exogenous nitric oxide on Brassica juncea seedlings under NaCl stress'. Biologia Plantarum, 55: 345-348.
Zhang, Y., L. Wang, Y. Liu, Q. Zhang, Q. Wei and W. Zhang. 2006. 'Nitric oxide enhances salt tolerance in maize seedlings through increasing activities of proton-pump and Na+/H+ antiport in the tonoplast'. Planta, 224: 545-555.
Zhao, M., X. Zhao, Y. Wu and L. Zhang. 2007. 'Enhanced sensitivity to oxidative stress in an Arabidopsis nitric oxide synthase mutant'. Journal of plant physiology, 164: 737-745.
