A comparative study on phytochemical potentials of Rubus loganobaccus L.

الموضوعات :

Saeede Ekbatan Hamadani ¹ (Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
Hossein Lari Yazdi ² (Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran)
Mohammad Hassan Asareh ³ (Research Institute of Forests and Rangelands, Tehran, Iran)
Sara Saadatmand ⁴ (Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)

تاريخ الإرسال : 02 السبت , رمضان, 1441 تاريخ التأكيد : 02 السبت , رمضان, 1441 تاريخ الإصدار : 06 الأربعاء , جمادى الأولى, 1441

الکلمات المفتاحية: DPPH, phytochemicals, Secondary metabolites, biochemical activity, Genus Rubus,

ملخص المقالة :

Raspberries composed of various varieties, are popular ingredients of daily diet with highly distinguished biological activities. In this study, a comparative investigation was conducted on various chemical potential of methanol extracts from Rubus loganobaccus L. leaf parts cultured in greenhouse and open-field. Biochemical activity of the extracts obtained from the field cultured leaves were observed to be higher than greenhouse cultured plants. In the antioxidant 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the field cultured leaves were better succeeded in radical scavenging with IC50 as low as 1.08 ± 0.75 μg/mL while in reducing power assay, open-field plants against greenhouse plants had a higher EC50 (2.82 ± 0.70 and 2.41 ± 0.75 μg/mL, respectively). Considerable antibacterial and antifungal activity were observed in open-field plants and greenhouse plants in minimum inhibitory concentrations (MIC) and minimum bacterial concentration (MBC) tests with a similar pattern, in which the lowest MIC and MBC in open-field and greenhouse plants were 5 mg/mL and 20 mg/mL, respectively, against Bacillus cereus and pseudomonas aeruginosa. This experiment revealed that leaf parts of Rubus loganobaccus cultured in greenhouse and in field possess a number of biological properties including antioxidative and antimicrobial potentials with the superiority of the later source which indicates the criticality of using natural products to improve human health.

المصادر:

Ahmadvand, H., H. Amiri, Z.D. Elmi and S. Bagheri. 2013. 'Chemical composition and antioxidant properties of ferula-assa-foetida leaves essential oil'. Iranian Journal of Pharmaceutical Research, 12(2): 55-57.

Bakhshipour, M., M. Mafakheri, M. Kordrostami, A. Zakir, N. Rahimi, F. Feizi and M. Mohseni. 2019. 'In vitro multiplication, genetic fidelity and phytochemical potentials of Vaccinium arctostaphylos L.: An endangered medicinal plant'. Industrial Crops and Products, 141, 111812.

Bobinaitė, R., P. Viškelis, A. Šarkinas and P.R. Venskutonis. 2013. 'Phytochemical composition, antioxidant and antimicrobial properties of raspberry fruit, pulp, and marc extracts'. CyTA-Journal of Food, 11(4):334-342.

Boeing, J.S., E.O. Barizao, P.F. Montanher, V. de Cinque Almeida and J. V. Visentainer. 2014. 'Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis'. Chemistry Central Journal, 24(1): 8-48.

Bondet, V., W. Brand-Williams and C. L. W. T. Berset. 1997. 'Kinetics and mechanisms of antioxidant activity using the DPPH free radical method'. LWT-Food Science and Technology, 30(6):609-615.

 Bonjar, G. S. 2004. 'Antibacterial screening of plants used in Iranian folkloric medicine'. Fitoterapia ,75(2):231-235.

Cavanagh, H. M., M. Hipwell ana J. M. Wilkinson. 2003. 'Antibacterial activity of berry fruits used for culinary purposes'. Journal of Medicinal Food, 6(1): 57-61.

Debnath, S. C. 2016. 'Genetic diversity and erosion in berries'. In Genetic Diversity and Erosion in Plants. Ahuja, M.R. and S. Mohan Jain (Eds.). Springer, Cham, pp: 75-129.

Halliwell, B., J. Rafter and A. Jenner. 2005. 'Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: direct or indirect effects? Antioxidant or not?'. The American Journal of Clinical Nutrition, 81(1): 268S-276S.

Hood, J. R., J. M. Wilkinson and H. M. Cavanagh. 2003. 'Evaluation of common antibacterial screening methods utilized in essential oil research'. Journal of Essential Oil Research, 15(6): 428-433.

Jorge, J. M., F. Pérez-García and V. F. Wendisch. 2017. 'A new metabolic route for the fermentative production of 5-aminovalerate from glucose and alternative carbon sources'. Bioresource technology, 245 (8):  1701-1709.

Mafakheri, M and Y. Hamidoghli. 2015. 'Effect of different extraction solvents on phenolic compounds and antioxidant capacity of hop flowers (Humulus lupulus L.)'. IV International Humulus Symposium, 1236: 1-6.

Nasiry, D., H. Ahmadvand, F. T. Amiri and E. Akbari. 2017. 'Protective effects of methanolic extract of Juglans regia L. leaf on streptozotocin-induced diabetic peripheral neuropathy in rats'. BMC Complementary and Alternative Medicine, 17(1), 476.     

Puupponen‐Pimiä, R., L. Nohynek, S. Hartmann‐Schmidlin, M. Kähkönen, M. Heinonen, K. Määttä‐Riihinen and K. M. Oksman‐Caldentey. 2005. 'Berry phenolics selectively inhibit the growth of intestinal pathogens'. Journal of Applied Microbiology, 98(4), 991-1000.

hibu, P.C.R and P.C. Pratab. 2017.'Phytochemical and antimicrobial analysis of leaf samples of different Rubus species'. International Journal of Chem. Tech Research, 10(4):359-368.

Singleton, V. L., R. Orthofer and R. M. Lamuela-Raventós. 1999. 'Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent'. In Methods in enzymology. Academic press, 299, pp.152-178

Vuorela, S., K. Kreander, M. Karonen, R. Nieminen, M. Hämäläinen, A. Galkin and H. Vuorela. 2005. 'Preclinical evaluation of rapeseed, raspberry, and pine bark phenolic for health related effects'. Journal of Agricultural and Food Chemistry, 53(15): 5922-5931.

Thompson, M. M. 1995. 'Chromosome numbers of Rubus species at the national clonal germplasm repository'. HortScience, 30(7): 1447-1452.

Yokozawa, T., C. P. Chen, E. Dong, T. Tanaka, G. I. Nonaka and I. Nishioka. 1998. 'Study on the inhibitory effect of tannins and flavonoids against the 1, 1-diphenyl-2-picrylhydrazyl radical'. Biochemical Pharmacology, 56(2): 213-222.

Skrovankova, S., D. Sumczynski, J. Mlcek, T. Jurikova and J. Sochor. 2015. 'Bioactive compounds and antioxidant activity in different types of berries'. International Journal of Molecular Sciences, 16(10): 24673-24706.