Single Nucleotide Polymorphisms of Mc4R Gene and Their Associations with Growth Traits in Karacabey Merino Lambs
الموضوعات :
S. Sevim
1
(Ministry of Agriculture and Forestry Aydın Provincial Directorate, Aydın, Türkiye)
O. Karaca
2
(Department of Animal Science, Faculty of Agriculture, University of Adnan Menderes, Aydın, Türkiye)
الکلمات المفتاحية: growth traits, DNA sequencing, KaracabeyiMerino, Mc4R,
ملخص المقالة :
Mc4R gene regulates feed intake, live weight, and energy balance in animals. It is one of growth and development candidate genes, as indicated by its role in energy balance and feed intake. The goal of the research was to analyze the polymorphisms of the Mc4Rxgene and their relations with growth characteristics in KaracabeyxMerino lambs. 300 Karacabey Merinoxlambs reared at the Bandırma Sheep Breeding Research Institute in Balıkesir, Turkiye, served as the research's animal subjects. The total of 1291 bp long exon and 3’UTR regions of Mc4Rigene was amplified. PCR products were analysed by DNA sequencing. A total of 6 SNP of Mc4R (g.9.T>C, g.12.G>C, g.93.G>A, g.381. G>A, g.681.G>C and g.1016. G>A) gene were identified. These SNP’s have not been inducing changes sequence of amino acids. The single nucleotide polymorphisms (SNPs) of Mc4R gene were found significant associations between muscle depth, skin thickness, back fat thickness, average daily gain and live weight. The Mc4R gene was shown to be a potential genetic marker in selection programs on sheep to improve growth traits of lambs.
Akyüz B., Arslan K. and İlgar E.G. (2017). Türkiye’de yetiştirilen anadolu mandalarında butirofilin (BTN1A1) gen polimorfizminin HaeIII restriksiyon enzimi ile araştırılması. Erciyes Üniv. Vet. Fak. Derg. 14(1), 11-16.
Atav R., Bugdaycı B., Şen A., Ergünay U., Ünal G.P., Ünal Ö.E., Karagöz G., Işık R., Soysal İ.M., Özder M., Arat S. and Eroğlu B. (2023). Creating a high-quality wool-oriented Turkish merino herd and investigation of mechanical and dyeability properties of fabrics produced from Turkish merino in comparison with Australian merino. Color Technol. 10, 1-14.
Bayraktar M. and Shoshin, O. (2021). Estimate of the association of IGF-I and IGFALS genes with growth traits in Hamdani sheep. Brazilian Arch. Biol. Technol. 64, 1-9.
Botstein D., White R.L., Skolnick M. and Davis R.W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32, 314-331.
Cai X., Mipam T.D., Zhao F.F. and Sun L. (2015). SNPs detected in the yak MC4R gene and their association with growth traits. Animal. 9(7), 1097-1103.
Cheng J., Xin Li X., Wang J., Zhou B., Lin C., Yang X. and Zhang X. (2022). Identification of polymorphic loci in OSMR and GHR genes and analysis of their association with growth traits in sheep. Anim. Biotechnol. 34(7), 2546-2553.
Cone R.D. (2005). Anatomy and regulation of the central melanocortin system. Nat. Neurosci. 8, 571-578.
Deng T.X., Pang C.Y., Liu M.Q., Zhang C. and Liang X.W. (2016). Synonymous single nucleotide polymorphisms in the MC4R gene that are significantly associated with milk production traits in water buffaloes. Genet. Mol. Res. 15, 1-8.
Dönmez O. (2008). Bursa İli koyunculuk işletmelerinin yetiştiricilik açısından yapısı. MS. Thesis. Tekirdağ, Turkey.
Feng J., Li H. and Yan C.G. (2017). The correlation analysis between the SNPs of MC4R gene and meat quality traits in Yanbian yellow cattle. China Anim. Husband. Vet. Med. 44, 1406-1413.
Grillo G., Turi A., Licciulli F., Mignone F., Liuni S., Banfi S., Gennarino V.A., Horner D., Pavesi G., Picardi E. and Pesole G. (2010). UTRdb and UTRsite (RELEASE 2010): A collection of sequences and regulatorymotifs of the untranslated regions of eukaryotic mRNAs. Nucleic Acids Res. 38, 75-80.
Klimenko A., Usatov A., Getmantseva L., Kolosov Y., Tretyakova O., Bakoev S., Kostjunina O. and Zinovieva N. (2014). Effects of melanocortin-4 receptor gene on growth and meat traits in pigs raised in Russia. Am. J. Agric. Biol. Sci. 9, 232-237.
Latifah L., Maharani D., Kustantinah A., and Hartatik T. (2018). Association of melanocortin 4 receptor gene polymorphism with growth traits in Bligon goat. J. Indonesian Trop. Anim. Agric. 43(4), 343-351.
Lee Y.S., Park S., Kim H., Lee S.K., Kim J.W., Lee H.K., Jeong D.K. and Lee S.J. (2013). A C1069G SNP of the MC4R gene and its association with economic traits in Korean native cattle (brown, brindle, and black). Elect. J. Biol. 16(5), 14-21.
Li W., Wang X., Zhang X., Li F., Zhang D., Li X., Zhang Y., Zhao Y., Zhao L., Xu D., Cheng J., Wang J., Zhou B., Lin C. and Wang W. (2023). Polymorphism of sheep PRKAA2 gene and its association with growth traits. Anim. Biotechnol. 34(4), 1324-1330.
Liu H., Tian W., Zan L., Wang H. and Cui H. (2010). Mutations of MC4R gene and its association with economic traits in Qinchuan cattle. Mol. Biol. Rep. 37(1), 535-540.
Markison S. and Foster A.C. (2007). Targeting melanocortin receptors for the treatment of obesity. Drug Discov. Today. 3, 569-576.
Polatoğlu M.G. (2019). Cumhuriyet döneminde hayvancılığın sanayiye tatbikine bir örnek: Merinos yetiştiriciliği. Atatürk Araşt. Merk. Derg. 100, 585-620.
Prihandini P.W. and Maharani D. (2019). Melanocortin‐4 receptor (MC4R) gene as the main gene for rapid growth selection in beef cattle. Agric. Food Sci. 29(2), 85-95.
Reamon-Buettner S.M., Cho S.H. and Borlak J. (2007). Mutations in the 30-untranslated region of GATA4 as molecular hotspots for congenital heart disease (CHD). BMC Med. Genet. 8, 38-45.
Safari E., Fagorty N.M. and Gilmour A.R. (2005). A review of genetic parameter estimates for wool, growth, meat and reproduction traits in sheep. Livest. Prod. Sci. 92(3), 271-289.
Seong J., Suh D.S., Do Park K., Lee H.K. and Kong H.S. (2012). Identification and analysis of MC4R polymorphisms and their association with economic traits of Korean cattle (Hanwoo). Mol. Biol. Rep. 39(4), 3597-3601.
Shan H., Song X., Cao Y., Xiong P., Wu J., Jiang J. and Jiang Y. (2020). Association of the melanocortin 4 receptor (MC4R) gene polymorphism with growth traits of Hu sheep. Small Rumin. Res. 192, 1-9.
Shibayama A., Cook E.H.J., Feng J., Glanzmann C., Yan J., Craddock N., Jones I.R., Goldman D., Heston L.L. and Sommer S.S. (2004). MECP2 structural and 3’-UTR variants in schizophrenia, autism and other psychiatric diseases: a possible association with autism. Am. J. Med. Genet. B. 128, 50-53.
Shishay G., Liu G., Jiang X., Yu Y., Teketay W., Du D., Jing H. and Liu C. (2019). Variation in the promoter region of the MC4R gene elucidates the association of body measurement traits in Hu sheep. Int. J. Mol. Sci. 20(2), 240-248.
Song X.M., Jiang J.F., Zhang G.Z., Shi F.X. and Jiang Y.Q. (2012). DNA polymorphisms of the Hu sheep melanocortin-4 receptor gene associated with birth weight and 45-day weaning weight. Genet. Mol. Res. 11, 4432-4441.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.
Türkiye Istatistik Kurumu (TUIK). (2023). https://biruni.tuik.gov.tr/medas/?kn=101&locale=en, Accessed Sep. 2023.
Wang Y., Wang C., Zhang J., Meng C., Zhang X., Wang Z., Fang Y., Mao D. and Cao S. (2015). Three novel MC4R snps associated with growth traits in Hu sheep and East Friesian Hu crossbred sheep. Small Rumin. Res. 125, 26-33.
Yeh F., Yang R.C. and Boyle T. (1999). Popgene (v.1.32): Microsoft Windows-based free ware for Population Genetic Analysis. Available at: http://www.ualberta.ca/~fyeh/popgene_download.html.
Yilmaz O., Sezenler T., Alarslan E., Ata N., Karaca O. and Cemal İ. (2014). Karacabey Merinosu, Karya ve Kıvırcık kuzularda sütten kesim döneminde kabuk yağı kalınlığı ve musculus longissmus dorsi thoracis et lumborum (MLD) derinliğinin ultrason ölçümleri. Kafkas Üniv. Vet. Fak. Derg. 20, 829-834.
Yuan M., Zhan Q., Duan X., Song B., Zeng S., Chen X., Yang Q. and Xia J. (2012). A functional polymorphism at miR-491-5p binding site in the 3’-UTR of MMP-9 gene confers increased risk for atherosclerotic cerebral infarction in a Chinese population. Atherosclerosis. 226(2), 447-452.
Zhai R., Wang W., Zhang D., Li X., Zhang Y., Zhao Y., Zhao L., Wang J., Xu D., Cheng J., Li W., Zhou B., Lin C., Zeng X., Yang X., Ma Z., Liu J., Cui P. and Zhang X.X. (2022). Novel polymorphism at KLF15 gene and its association with growth traits in Hu sheep. Anim. Biotechnol. 2022, 1-7.
Zhao X.L., Cao S.J. and Wang J.Q. (2018). Association analysis of MC4R gene polymorphisms and genotype combination with growth traits of Tibetan Sheep (Ovis aries). J. Agric. Biotechnol. 26(3), 429-436.
Zuo B., Liu G., Peng Y., Qian H., Liu J., Jiang X. and Mara A. (2014). Melanocortin-4 receptor (MC4R) polymorphisms are associated with growth and meat quality traits in sheep. Mol. Biol. Rep. 41, 6967-6974.
