Polymorphism of the Growth Hormone Gene and Its Effect on Production and Reproduction Traits in Goat
الموضوعات :ف. غلامحسین زاده گوکی 1 , م.ر. محمدآبادی 2 , م. اسدی فوزی 3
1 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
2 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
3 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
الکلمات المفتاحية: PCR-RFLP, Growth hormone, birth and fleece weight, birth type, Raini Cashmere goat,
ملخص المقالة :
Growth hormone (GH) has an effect on a broad variety of physiological parameters such as lactation, reproduction, growth and metabolism. Thus, the aim of this study was to detect GH gene polymorphism and its association with breeding values of production and reproduction traits in Raini Cashmere goat. Breeding values were estimated using records on 26731 Raini Cashmere goats. To study GH gene polymorphism, 300 animals were selected based on their estimated breeding values (EBVs) for these traits. Then the animal’s genotype was determined using PCR-RFLP. The genotype frequencies for AA, AB and BB were 0.15, 0.85 and 0, respectively. The number of observed alleles, number of effective alleles, expected heterozygosity, observed heterozygosity, mean of heterozygosity, expected homozygosity, observed homozygosity, Nei’s index, Shanon’s index and Fixation index (Fis) were 2, 1.96, 0.49, 0.85, 0.49, 0.51, 0.15, 0.49, 0.69 and -0.74, respectively. Results showed that mean estimated breeding values for birth type, fleece weight and birth weight traits in different genotypes varies, of course these differences were not statistically significant (P>0.05). However, for fleece weight and birth type traits AB genotype had higher EBV. Due to the relatively high diversity of growth hormone gene in Raini Cashmere goat and its association with important economic traits, using growth hormone gene in breeding programs of this breed can lead to acceptable genetic progress and applying AA genotype for birth weight trait and AB genotype for fleece weight and birth type traits can be used as an indirect marker for selection of superior animals.
Allain D. and Roguet J. (2003). Genetic and nongenetic factors influencing mohair production traits within the national selection scheme of Angora goats in France. Livest. Prod. Sci. 82, 129-137.
Askari N., Baghizadeh A. and Mohammadabadi M.R. (2008). Analysis of the genetic structure of Iranian indigenous Raeni Cashmere goat populations using microsatellite markers. Biotechnology. 2, 1-4.
Askari N., Baghizadeh A. and Mohammadabadi M.R. (2010). Study of genetic diversity in four populations of Raeini Cashmere goat using ISSR markers. Mod. Genet. J. 5, 49-56.
Askari N., Mohammadabadi M.R., Beygi Nassiry M.T., Baghizadeh A. and Fayazi J. (2009). Study of genetic diversity of Raeini Cashmere goat based on microsatellite markers. J. Agric. Sci. 18, 155-161.
Baghizadeh A., Bahaaddini M., Mohamadabadi M.R. and Askari N. (2009). Allelic variations in exon 2 of Caprine MHC class II DRB3 gene in Raeini Cashmere goat. American J. Agric. Environ. Sci. 6, 454-459.
Bharathidhasan A., Narayanan R., Gopu P., Subramanian A., Prabakaran R. and Rajendran R. (2009). Effect of nongenetic factors on birth weight, weaning weight and preweaning gain of Barbari goat. J. Vet. Anim. Sci. 5, 99-103.
Boujenane I. and Hazzab A.E. (2008). Genetic parameter for direct and mater effect on body weight of Draa goat. Small Rumin. Res. 69, 149-156.
Chungyan Z., Ligu Y. and Zhang S. (2008). Variance components and genetic parameters for weight and size at birth in Boer goat. Livest. Prod. Sci. 115, 73-79.
Emami Meybodi M.A. (1993). The estimate genetic parameters for some economic traits in Raieni Cashmere goats. MS Thesis. Ferdowsi Univ., Mashhad, Iran.
Falaki M., Prandi A., Corradini C., Sneyers M., Massart S., Fazzini U., Burny A., Portetelle D. and Renaville R. (1997). Relationships of growth hormone gene and milk protein polymorphisms to milk production traits in Simmental cattle. J. Dairy Res. 64, 47-56.
FAO. (2008). ProdSTAT livestoch primary and processed. Food and Agriculture Organization of the United Nations (FAO), Rome, Italy.
Glimur A.R., Gogel B.J., Cullis B.R. and Thompson R. (2009). ASReml user guide. NSW Department of Industry and Investment, VSN International Ltd., Hemel Hempstead, United Kingdom.
Hassani M.N., Asadi Fozi M., Esmailizadeh A.K. and Mohammadabadi M.R. (2010). A genetic analysis of growth traits in raieni Cashmere goat using multivariate animal model. Iranian J. Anim. Sci. 41, 323-329.
Hermiz H.N., Alkass J.E., Hobi A.A. and Asofi M.K. (2009). Genetic and phenotypic parameters of body weights in Iraqi local goat and their crosses with Damascus. J. Univ.Duhok. 12, 189-194.
Hua G.H., Chen S.L., Yu J.N., Cai K.L., Wu C.J., Li Q.L., Zhang C.Y., Liang A.X., Hana L., Geng L.Y., Shen Z., Xu D.Q. and Yang L.G. (2009). Polymorphism of the growth hormone gene and its association with growth traits in Boer goat bucks. Meat Sci. 81, 391-395.
Kioka N., Manabe E., Abe M., Hashi H., Yato M., Okuno M., Yamano Y., Sakai H., Komano T., Utsumi K. and Iritani A. (1989). Cloning and sequencing of goat growth hormone gene. Agric. Biol. Chem. 53, 1583-1587.
Lan X.Y., Chen H., Pan C.Y., Ming L.J., Lei C.Z., Hua L.S., Zhang C.L. and Hu S.R. (2007). Polymorphism in growth hormone gene and its association with production traits in goats. J. Appl. Anim. Res. 32, 55-60.
Malveiro E., Pereira M., Marques P.X., Santos I.C., Belo C., Renaville R. and Cravador A. (2001). Polymorphisms at the five exons of the growth hormone gene in the Algarvia goat: Possible association with milk traits. Small Rumin. Res. 41, 163-170.
Marques P.X., Pereira M., Marques M.R., Santos I.C., Belo C.C., Renaville R. and Cravador A. (2003). Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat. Small Rumin. Res. 50, 177-185.
Miller S.A., Dykes D.D. and Polesky H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res. 16, 1215-1220.
Moghadaszadeh M., Mohammadabadi M.R. and Esmailizadeh A.K. (2015). Association of exon 2 of BMP15 gene with the litter size in the Raini Cashmere goat. Genet. 3rd Millennium. 13, 4062-4067.
Mohammadabadi M.R. (2012). Relationships of IGFBP-3 gene polymorphism with Cashmere traits in Raini Cashmere goat. Mod. Genet. J. 7, 115-120.
Mohammadabadi M.R., Torabi A., Tahmourespoor M., Baghizadeh A., Esmailizadeh A.K. and Mohammadi A. (2010a). Analysis of bovine growth hormone gene polymorphism of local and Holstein cattle breeds in Kerman province of Iran using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). African J. Biotechnol. 9, 6848-6852.
Mohammadabadi M.R., Nikbakhti M., Mirzaee H.R., Shandi A., Saghi D.A., Romanov M.N. and Moiseyeva I.G. (2010b). Genetic variability in three native Iranian chicken populations of the Khorasan province based on microsatellite markers. Russian J. Genet. 46, 505-509.
Mohebi Nejad E. and Asadi Fozi M. (2012). Effect of environmental factors on fleece weight of Raeini Cashmere goats. J. Anim. Sci. Res. 22, 1-9.
Mousavizadeh A., Mohammad Abadi M.R., Torabi A., Nassiry M.R., Ghiasi H. and Esmailizadeh A.K. (2009). Genetic polymorphism at the growth hormone locus in Iranian Talli goats by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Iranian J. Biotechnol. 7, 51-53.
Restall B.J. and Pattie W.A. (1989). The inheritance of Cashmere in Australian goats. 2. Genetic parameters and breeding value. Livest. Prod. Sci. 21, 251-261.
Ruzina M.N., Shtyfurko T.A., Mohammadabadi M.R., Gendzhieva O.B., Tsedev T. and Sulimova G.E. (2010). Polymorphism of the BoLA-DRB3 gene in the Mongolian, Kalmyk, and Yakut cattle breeds. Russian J. Genet. 46, 456-463.
Saleha Y.M.A., Karima F.M., Lamiaa M.S. and Ekram S.A. (2012). Genetic polymorphism of five genes associated with growth traits in goat. African J. Biotechnol. 11, 14738-14748.
Schlee P., Graml R., Schallenberger E., Schams D., Rottmann O., OlbrichBludau A. and Pirchner F. (1994). Growth hormone and insulin-like growth factor І concentrations in bull of various growth hormone genotypes. Theor. Appl. Genet. 88, 497-503.
Shamsalddini S., Mohammadabadi M.R. and Esmailizadeh A.K. (2016). Polymorphism of the Prolactin gene and its effect on fiber traits in goat. Russian J. Genet. 52, 405-408.
Singh P.P., Tomar S.S., Thakur M.S. and Kumar A. (2015). Polymorphism and association of growth hormone gene with growth traits in Sirohi and Barbari breeds of goat. Vet. World. 8, 382-387.
Soufy B., Mohammadabadi M.R., Shojaeyan K., Baghizadeh A., Ferasaty S., Askari N. and Dayani O. (2009). Evaluation of Myostatin gene polymorphism in Sanjabi sheep by PCR-RFLP method. Anim. Sci. Res. 19, 81-89.
Tohidi Nezhad F., Mohammadabadi M.R., Esmailizadeh A.K. and Najmi Noori A. (2015). Comparison of different levels of Rheb gene expression in different tissues of Raini cashmir goat. J. Agric. Biotechnol. 6, 35-50.
Valinsky A., Shani M. and Gootwine E. (1990). Restriction fragment length polymorphism in sheep at the growth hormone locus is the result of variation in gene number. Anim. Biotechnol. 1, 135-144.
Walkden-brown S., Sunduimijid B., Olayemi B., van der Werf J. and Ruvinsky A. (2008). Breeding fiber goat for resistance to worm infections. Pp. 70-87 in Proc. Rural Indust. Res. Dev. Corp., University of New England, Armidale, Australia.
Yamano Y., Oyabayashi K., Okuno M., Yato M., Kioka N., Manabe E., Hashi H., Sakai H., Komano T., Utsumi K. and Iritani A. (1988). Cloning and sequencing of cDNA that encodes goat growth hormone. FEBS Lett. 228, 301-304.
Yato M., Yamano Y., Oyabayashi K., Okuno M., Yato M., Kioka N., Manabe E., Hashi H., Sakai H., Komano T., Utsumi K. and Iritani A. (1988). Nucleotide sequence of the growth hormone gene cDNA from goat Caprahircus (Tokara). Nucleic Acids Res. 16, 3578-3585.
Yeh F.C., Yang R.C. and Boyle T. (1999). POPGENE, Microsoft Windowsbased Freeware for Population Genetic Analysis. Release 1.31. University of Alberta, Canada.
Zhang C., Liu Y., Huang K., Zeng W., Xu D., Wen Q. and Yang L. (2011). The association of two single nucleotide polymorphisms (SNPs) in growth hormone (GH) gene with litter size and superovulation response in goat-breeds. Genet. Mol. Biol. 34, 49-55.
Zhang H.M., Brown D.R., DeNise S.K. and Ax R.L. (1992). Nucleotide sequence determination of a bovine somatotropin allele. Anim. Genet. 23, 578-587.
