Influence of Dietary Thyme Extract (Thymus vulgaris) on Performance, Purine Derivatives, Cellulase Activity and Ruminal Fermentation Parameters in Fattening Lambs and Goat Kids
الموضوعات :S. Shahravan 1 , T. Ghoorchi 2 , B. Dastar 3 , A.H. Toghdori 4 , M. Mohajer 5
1 - Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
2 - Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
3 - Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
4 - Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
5 - Department of animal and Poultry Nutrition and Physiology Research, Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
الکلمات المفتاحية: microbial protein, Ruminal fermentation, Thyme extract, Enzyme activities,
ملخص المقالة :
This study was investigated to compare the effects of thyme extract (Thymus vulgaris) on performance, ruminal fermentation, cellulase enzyme activity and microbial protein synthesis in fattening lambs and goat kids. For this purpose, 15 kids (average initial BW of 17.3±1.2 kg, 3 month-old) and 15 lambs (average initial BW of 21.4±1.5 kg, 3 month-old) were randomly assigned to 3 dietary treatment: 1) control (without thyme extract), 2) supplemented with 250 mg thyme extract, 3) supplemented with 500 mg thyme extract. Animals were housed in individual pens for 84 days. Experimental results showed that different levels of thyme extract had a significant effect on daily weight gain and feed intake in both species, so that in animals fed 250 mg of thyme extract was higher than the other treatments (P<0.01). Rumen pH was not affected by experimental treatments. The lowest NH3-N was observed in the treatment containing 250 mg of thyme extract (P<0.01). The total concentrations of volatile fatty acids (VFA) as well as butyrate and acetate in ruminal fluid decreased significantly with increasing thyme extract; but propionate was increased in kids and lambs fed with 250 mg of thyme extract (P<0.01). Microbial protein produced in the rumen were significantly affected by experimental treatments (P<0.01). The activity of cellulolytic enzymes was not affected by the treatments. Our findings indicate that 250 mg thyme extract can be used in diet without adverse effect on lambs and kids. The lambs were also more affected by the thyme extract than the kids.
Abdullah M., Baber M.E., Jabbar M.A., Javed K. and Nasir M. (2013). Performance of Beetal goats and Lohi sheep under different feeding management systems. Pakistan J. Zool. 45, 107-11.
Acamovi T. and Brooker J.D. (2005). Biochemistry of plant secondary metabolites and their effects in animals. Proc. Nutr. Soc. 64, 403-412.
Agarwal N. (2000). Estimation of fiber degrading enzyme. Pp. 278–291 in Feed Microbiology. L.C. Chaudhary, N. Agarwal, D.N. Kamra and D.K. Agarwal, Eds., CAS Animal Nutrition, IVRI, Izatnagar, India.
Agarwal N., Agarwal I., Kamra D.N. and Chaudhary L.C. (2000). Diurnal variations in the activities of hydrolytic enzymes in different fractions of rumen contents of murrah buffalo. J. Appl. Anim. Res. 18, 73-80.
AOAC. (2000). Official Methods of Analysis. 17th Ed. Association of Official Analytical Chemists, Arlington, Washington, DC., USA.
Baytok E., Kara K., Aks T., Guclu B.K., Ozkaya S., Denek N., Kamalak A., Kaya D.A., Onel S.E. and Akcay A. (2017). The effect of Mediterranean thyme (Thymbra spicata L. var. spicata) essential oil on fattening performance and ruminal parameters in lamb. J. Anim. Feed Sci. 26, 319-325.
Benchaar C., Calsamiglia S., Chaves A.V., Fraser G.R., Colombatto D., McAllister T.A. and Beauchemin K.A. (2008). A review of plant-derived essential oils in in ruminant nutrition and production. Anim. Feed Sci. Technol. 145, 209 228.
Biricik H., Oral H.H., Taluğ A.M., Cengiz Ş.Ş., Koyuncu M. and Dikmen S. (2016). The effects of carvacrol and/or thymol on the performance, blood and rumen parameters, and carcass traits of Merino sheep. Turkish J. Vet. Anim. Sci. 40, 651-659.
Broderick G. and Kang J.H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J. Dairy Sci. 54, 1176-1183.
Budak D. and Yılmaz A. (2013). Effects of aromatic plants on rumen fermentation. Macedonian J. Anim. Sci. 3(1), 75-80.
Calsamiglia S., Busquet M., Cardozo P.W., Castillejos L. and Ferret A. (2007). Invited review: Essential oil as modifiers of rumen microbial fermentation. J. Dairy Sci. 90, 2580-2595.
Candyrine S.C.L., Jahromi M.F., Ebrahimi M., Chen W.L., Rezaei S., Goh Y.M., Abdullah N. and Liang J.B. (2019). Oil supplementation improved growth and diet digestibility in goats and sheep fed fattening diet. Asian-Australasian J. Anim. Sci. 32(4), 533-540.
Candyrine S.C.L., Liang J.B. and Goh Y.M. (2016). In vitro rumen fermentation characteristics of goat and sheep supplemented with polyunsaturated fatty acids. Anim. Prod. Sci. 57, 1607-1612.
Chaves A.V., Stanford K., Gibson L., McAllister T.A. and Benchaar C. (2008). Effects of carvacrol and cinnamaldehyde on intake, rumen fermentation, growth performance, and carcass characteristics of growing lambs. Anim. Feed Sci. Technol. 145, 396-408.
Chen X.B. and Gomes J.M. (1992). Estimation of Microbial Protein Supply to Sheep and Cattle Based on Urinary Excretion of Purine Derivatives: An Overview of the Technical Details. Occasional Publication of the International Feed Resources Unit, Rowett Res. Inst., Bucksburn, Aberdeen, United Kingdom.
Erwin E.S., Marco G.J. and Emery E.M. (1961). Volatile fatty acid analyses of blood rumen fluid by gas chromatography. J. Dairy Sci. 44, 1768-1771.
Evans J.D. and Martin S.A. (2000). Effects of thymol on ruminal microorganisms. Curr. Microbiol. 41, 336-340.
Ganjkhanlou M., Hozhabri A., Zali A., Emami A. and Akari Afjanh A. (2014). Effect of fish oil and Thyme extract on dry matter and nutrient digestibility, chewing activity and rumen metabolites of Mahabadi kids. Res. Anim. Prod. 5(10), 69-83.
Hart K.J., Y´a.nez-Ruiz D.R., Duval S.M., McEwan N.R. and Newbold C.J. (2008). Plant extracts to manipulate rumen fermentation. Anim. Feed Sci. Technol. 147(8), 35-44.
Hristov A.N., McAllister T.A. and Cheng K.J. (1999). Effect of diet, digesta processing, freezing and extraction procedure on some polysaccharide degrading activities of ruminal contents. Canadian J. Anim. Sci. 79, 73-81.
Hu W.L., Liu J.X., Ye J.A., Wu Y.M. and Guo Y.Q. (2005). Effect of tea saponin on rumen fermentation in vitro. Anim. Feed Sci. Technol. 120, 333-339.
Khamisabadi H., Kafilzadeh F. and Charaen B. (2016). Effect of thyme (Thymus vulgaris) or peppermint (Mentha piperita) on performance, digestibility and blood metabolites of fattening Sanjabi lambs. Biharean Biol. 10(2), 118-122.
Khorrami B., Vakili A.R., Danesh Mesgaran M. and Klevenhusen F. (2015). Thyme and cinnamon essential oils: Potential alternatives for monensin as a rumen modifier in beef production systems. Anim. Feed Sci. Technol. 200, 8-16.
Leung A.Y. and Foster S. (1996). Encyclopedia of Common Natural Ingredients Used in Foods, Drugs and Cosmetics. Wiley and Sons, Inc., New Jersey, United States.
Makkar H.P.S. (2010). In vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies. Springer, New York.
Mao H.L., Wang J.K., Zhou Y.Y. and Liu J.X. (2010). Effects of addition of tea saponins and soybean oil on methane production, fermentation and microbial population in the rumen of growing lambs. Livest. Sci. 129, 56-62.
McGuffey R.K., Richardson L.F. and Wilkinson J.I.D. (2001). Ionophores for dairy cattle: Current status and future outlook. J. Dairy Sci. 84, 194-203.
McIntosh F.M., Williams P., Losa R., Wallace R.J., Beever D.A. and Newbold C.J. (2003). Effects of essential oils on ruminal microorganisms and their protein metabolism. Appl. Environ. Microbiol. 69, 5011-5014.
Miller G.L. (1959). Modified DNS method for reducing sugars. Anal. Chem. 31, 426-428.
NRC. (2007). Nutrient Requirements of Sheep. National Academy Press, Washington, DC, USA.
Newbold C.J., McIntosh F.M., Williams P., Riccardo L. and Wallace R.J. (2004). Effects of a specific blend of essential oil compounds on rumen fermentation. Anim. Feed Sci. Technol. 114, 105-112.
Okoruwa M.I. and Edoror O.M. (2019). Effect of garlic oil supplementation on intake, digestibility, performance and rumen function of goats fed silage based-diet. European J. Food Res. Rev. 10(2), 104-115.
Özdoğan M., Önenç S.S. and Önenç A. (2011). Fattening performance, blood parameters and slaughter traits of Karya lambs consuming blend of essential oil compounds. African J. Biotechnol. 10(34), 6663-6669.
Patra A.K. (2011). Effect of essential oil on rumen fermentation, microbial ecology and ruminal production. Asian J. Anim. Vet. Adv. 6(5), 416-428.
Patra A.K., Kamra D.N. and Agarwal N. (2009). Effects of extracts of spices on rumen methanogenesis, enzyme activities and fermentation of feeds in vitro. J. Sci. Food Agric. 90, 511-520.
Perdok H., Langhout P. and Vugt P.V. (2003). Stimulating appetite. Feed Mixture. 11, 10-13.
Pulina G., Avondo M., Molle G., Francesconi A.H.D., Atzori A.S. and Cannas A. (2013). Invited review: Models for estimating feed intake in small ruminants. R. Bras. Zootec. 42(9), 675-690.
SAS Institute. (2003). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Shipley L.A. (1999). Grazers and browsers: How digestive morphology affects diet selection. Pp. 20-27 in Grazing behavior of livestock and wildlife. K.L. Launchbaugh, K.D. Sanders and J.C. Mosley, Eds., University of Idaho, Moscow, Russia.
Tedeschi L.O., Fox D.G. and Tylutki T.P. (2003). Potential environmental benefits of ionophores in ruminant diets. J. Environ. Qual. 32, 1591-1602.
Tekeli A., Çelik L. and Kutlu H.R. (2007). Plant extracts; a new rumen moderator in ruminant diets. J. Tekirdag Agric. Fac. 4(1), 51-62.
Vaithiyanathan S., Bhatta R., Mishra A.S., Prasad R., Verma D.L. and Singh N.P. (2007). Effect of feeding graded levels of prosopis cineraria leaves on rumen ciliate protozoa, nitrogen balance and microbial protein synthesis in lambs and kids. Anim. Feed Sci. Technol. 133, 177-191.
Van Soest P.J., Robinson J.B. and Lewis B.A. (1991). Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583-3597.
Wallace R.J. (2004). Antimicrobial properties of plant secondary metabolites. Proc. Nutr. Soc. 63, 621-629.
Wallace R.J., Arthaud L. and Newbold C.J. (1994). Influence of Yucca-shidigera extract on ruminal ammonia concentrations and ruminal microorganisms. Appl. Environ. Microbiol. 60, 1762-1767.
Wanapat M., Pichad K., Pawadee P. and Sadudee W. (2008). Effect of supplementation of garlic powder on rumen ecology and digestibility of nutrients in ruminants. J. Sci. Food Agric. 88(13), 2231-2237.
Wang C.J., Wang S.P. and Zhou H. (2009). Influences of flavomycin, ropadiar, and saponin on nutrient digestibility, rumen fermentation and methane emission from sheep. Anim. Feed Sci. Technol. 148, 157-166.
Wang S.P. and Wang W.J. (2016). Effects of dietary supplementation of Chinese herb medicine mixture on rumen fermentation, nutrient digestion and blood profile in goats. South African J. Anim. Sci. 46, 247-260.
Wina E., Muetzel S. and Becker K. (2005). The dynamics of major fibro lytic microbes and enzyme activity in the rumen in response to short- and long-term feeding of Sapindus rarak saponins. J. Appl. Microbiol. 100, 114-122.
Yesilbag D., Biricik H., Cetin I., Kara C., Meral Y., Cengiz S.S., Orman A. and Udum D. (2016). Effects of juniper essential oil on growth performance, some rumen protozoa, rumen fermentation and antioxidant blood enzyme parameters of growing Saanen kids. J. Anim. Physiol. Anim. Nutr. 101(5), 1-10.
Zhou Y.Y., Mao H.L., Jiang F., Wang J.K., Liu J.X. and McSweeney C.S. (2011). Inhibition of rumen methanogenesis by tea saponins with reference to fermentation pattern and microbial communities in Hu sheep. Anim. Feed Sci. Technol. 166, 93-100.
