Semen Ejaculates Characteristics, in vitro Fertility and Their Interrelationships in Sahiwal Bull Semen
الموضوعات :
1 - Department of Veterinary Gynaecology and Obstetrics, Faculty of Veterinary and Animal Science, WBUAFS, 37, K.B. Sarani, Kolkata, 700037, West Bengal, India
2 - Department of Veterinary Gynaecology and Obstetrics, Faculty of Veterinary and Animal Science, WBUAFS, 37, K.B. Sarani, Kolkata, 700037, West Bengal, India
الکلمات المفتاحية: Semen, hypo-osmotic swelling test, physico-morphological, Sahiwal, sperm penetration distance, <i>in vitro</i> fertility,
ملخص المقالة :
This study aimed at determining the physico-morphological attributes, in vitrofertilityand their interrelationships in semen of Sahiwal bulls. Semen samples werecollected weekly for 6 weeks from ten mature Sahiwal bulls divided randomly into 5 groups. The mean (±SEM) of ejaculate volume (mL), mass activity (score 0-5), progressive sperm motility (%), sperm concentration (106/mL), live sperm (%), abnormal sperm (%), intact acrosome (%), hypo-osmotic swelling (HOS) positive sperm (%), sperm penetration distance (SPD, mm) were 4.28, 3.17, 76.73, 1185.53, 83.37, 9.97, 82.70, 79.00 and 56.85, respectively. The bulls varied significantly (P<0.05) in all their ejaculates characteristics Moreover, HOS positive sperm also had significant (P<0.01) positive correlation with mass activity (r=0.756), progressively motile sperm (r=0.374), live sperm (r=0.395) and SPD (r=0.681), and negative correlation with abnormal sperm (r=-0.463). Sperm penetration distance has significant (P<0.05) positive correlation with mass activity (r=0.776), progressively motile sperm (r=0.653), sperm concentration (r=0.365) and HOS positive sperm (r=0.681), and negative correlation with intact acrosome (r=-0.415).Overall semen quality of Sahiwalbulls was found optimum for use in breeding programme.
AlexanderN.J. (1981). Evaluation of male infertility with an in vitro cervical mucus penetration test. Fertil. Steril. 36, 201-208.
AnilkumarR., Devanathan T.G., Pattabiraman S.R. and Edwin J.M. (2001). Correlation between the spermatozoal characteristics and sperm penetration distance in polyacrylamide gel and bovine cervical mucus. Theriogenology. 55, 685-691.
AxR.L., Dally M. and Didion B.A. (2000). Semen evaluation, Pp. 365-375 in Reproduction in Farm Animals. B. Hafez and E.S.E. Hafez Eds. 7th Ed. Philadelphia: Lippincott Williams and Wilkins.
BalsPratsch M., Knuth U.A. and Nieschlag E. (1988). Does sperm penetration in bovine mucus provide additional information to the "classical" spermiogram and motility characteristics?Zentralbl Gynakol. 110(4), 224-231.
BelorkarP.M., Dhami A.J. and Kodagali S.B. (1988). Leakage of transaminases during freezing of cross-bred bulls semen. Indian Vet. J. 65(9), 809-812.
BelorkarP.M., Dhami A.J. and Kodagali S.B. (1991). Physiological norms and correlations of seminal characteristics, biochemical constituents, freezability and fertility in crossbred bulls. Indian. Vet. Med. J. 15, 191-197.
BhavsarB.K., Dhami A.J. and Kodagali S.B. (1990). Abnormal sperm content in Mehsana buffalo bulls’ semen with regard to freezability, seasonality and fertility. Indian Vet. J. 67(3), 233-237.
BritoL.F.C., Barth A.D., Bilodeau S., Panich P.L. and Kastelic J.P. (2003). Comparison of methods to evaluate the plasmalemma of bovine sperm and their relationship with in vitro fertilization rate. Theriogenology. 60, 1539-1551.
ChanderR., Bugalia N.S., Karwasra S., Garg S.L. and Agrawal V.K. (2004). Testosterone profiles and seminal characteristics in GnRH treated cow bulls. Indian. J. Anim. Sci. 74, 180-181.
CorreaJ.R., Pace M.M. and Zavos P.M. (1997). Relationship among frozen-thawed sperm characteristics assessed via the routine semen analysis, sperm functional tests and fertility of bulls in an artificial insemination program. Theriogenology. 48, 721-731.
DahlinA., Khan U.N., Zafar A.H., Saleem M., Chaudhry M.A. and Philipsson J. (1998). Genetic and environmental causes of variation in milk production traits of Sahiwal cattle in Pakistan. Anim. Sci. 66, 307-318.
DhamiA.J. and Sahni K.L. (1994). Comparative appraisal of physico-morphological and enzymatic attributes of semen and their interrelationships in ox and buffalo. J. Appl. Anim. Res. 5, 13-20.
DhamiA.J., Mohan G. and Sahni K.L. (1998). Seasonal influence on the quality and freezability of semen of Friesian and Murrah buffalo bulls. Indian J. Anim. Reprod. 19, 55-58.
DonnellyE.T., Lewis S.E.M., Mc Nally J. and Thompson W. (1998). In vitro fertilization and pregnancy rates: the influence of sperm motility and morphology on IVF outcome. Fertil. Steril. 70, 305-314.
EnglandG.C. and Plummer J.M. (1993). Hypoosmotic swelling of dog spermatozoa. J. Reprod. Fertil. 47, 261-270.
GopalkrishnaT. and Rao A.R. (1979). Correlation between semen characteristics and fertility in Murrah buffalo bulls. Ind. Vet. J. 56, 491-493.
GreenD.P.L. (1988). Sperm thrusts and the problem of penetration. Biol. Rev. 63, 79-107.
JavedM.T., Khan A. and Kausar R. (2000). Effect of age and season on some semen parameters of Nili Ravi buffalo (Bubalus bubalis) bulls. Vet. Archiv. 70, 83-94.
JayendranR.S., Vandervent H.H., Perez Peleae Z.M., Crabo B.G. and Zanevald L.J.D. (1984). Developement of an assay to assess the functional integrity of human sperm membrane and its relationship to their semen characteristics. J. Reprod. Fertil. 70, 219-225.
KeelB.A. and Schalue T.K. (2000). Correlation of the bovine cervical mucus penetration test with human sperm characteristics in 1406 ejaculates. Arch. Androl. 44, 109-115.
KremerJ. (1980). In vitro sperm penetration in cervical mucus and AIH. Pp. 30-37 in Homologous Artificial Insemination. J.C. Emperaire, A. Andebert and E.S.E. Hafez Eds. Martinus Hijhoss, The Hague, Boston, London.
KumarR.A. and Devnathan T.G. (1996). Effect of spermatozoal quality on sperm progression speed in cervical mucus. Indian Vet. J. 73, 645-648.
Kumi-Diaka J. (1993). Subjecting canine semen to the hypoosmotic test. Theriogenology. 39,1279-1289.
LarsonB. and Rodriguez Martinez H. (2000). Can we use in vitro fertilization tests to predict semen fertility? Anim. Reprod. Sci. 60, 327-336.
LasleyJ.F. (1951). Spermatozoa motility as a measure of semen quality. J. Anim. Sci. 10, 211-218.
MortimerD., Pandey I.J. and Sawers R.S. (1986). Relationship between human sperm motility characters and sperm penetration into human cervical mucus in vitro. J. Reprod. Fertil. 78, 93-102.
MuraseT., Mukohjima K., Sakaguchi S., Ohtani T., Tsubota T. and Kitai I. (2001). Influence of the different batches of estrous cervical mucus mixed for homogenization on penetration by spermatozoa in cattle. J. Reprod. Dev. 47, 109-112.
NayT. and Hayman R.H. (1956). Sweat glands in zebu and European cattle. 1. Size of individual glands.Aust. J. Agric. Res. 7, 482-494.
Nazir M. (1988). Semen evaluation and sperm morphology. Monograph on reproductive pattern of riverine buffaloes and recommendations to improve their reproductive performance at small farmer level. Pakistan Agric. Res. Council, Islamabad, Pakistan.
PatelK.V., Dhami A.J. and Kodagali S.B. (1989a). Seminal characteristics and their inter-relationships in K × J and K × HF crossbred bulls. Indian Vet. J. 66, 737-743.
PatelK.V., Dhami A.J., Derashri H.J. and Kodagali S.B. (1989b). Seminal biochemical profile and their interrelationships in crossbred bulls. Indian J. Anim. Sci. 59, 524-529.
RajuM.S. and Rao A.R. (1982). Note on the semen characteristics of crossbred and purebred bulls. Indian J. Anim. Sci. 52, 1230-1232.
RanaC.M. and Dhami A.J. (2003). Acrosomal integrity and HOS response at initial, post thaw and post refrigeration stages in sephadex filtered Gir (Bos indicus) and Jafarabadi (Bubalus bubalis) bulls’ semen. Indian J. Anim. Reprod. 24, 113-120.
RaoT.T., Rao M.M., Rao K.B. and Naidu K.V. (2011). Semen ejaculates characteristics and fertility in Ongole bulls. Indian Vet. J. 88(2), 36-37.
RavalR.J. and Dhami A.J. (2006). Physico-biochemical attributes of semen and their interrelationships in triple crossbred (HF X J X K) Bulls. Int. J. Cow. Sci. 2(2), 34-40.
RobayoI., Montenegro V., Valdes C. and Cox J.F. (2008). CASA assessment of kinematic parameters of ram spermatozoa and their relationship to migration efficiency in ruminant cervical mucus. Reprod. Domest. Anim. 43, 393-399.
Salisbury G.W., Vandemark N.L. and Lodge J.R. (1978). Physiology of Reproduction and Artificial Insemination of Cattle, 2nd Ed. W.H. Freeman and Co. San Francisco.
SaxenaV.B. and Tripathi S.S. (1978). Studies on the physicochemical attributes and preservability of semen of crossbred bulls. Indian J. Anim. Sci. 48, 865-869.
SaxenaV.B. and Tripathi S.S. (1979). Cholesterol content in the semen of Jersey, Red Dane and Murrah bulls. Indian J. Anim. Res. 13, 12-14.
ShanmugavelS. and Singh S.V. (2002). Physical and biochemical constituents of Sahiwal bull semen in relation to their quality. Ind. J. Anim. Sci. 72, 57-58.
Shelke V.B. and Dhami A.J. (2001). Comparative evaluation of physico-morphological attributes and freezability of semen of Gir cattle (Bos indicus) and Jafarabadi buffalo (Bubalus bubalis) bulls. Indian J. Anim. Sci. 71, 319-324.
SidhuK.S. and Guraya S.S. (1985). Buffalo Bull Semen Morphology, Biochemistry, Physiology and Methodology. USG Publishers and Distributors.
SinghS.P., Pandit R.K. and Bhadoriya H.B.S. (2000). Sexual behaviour and seminal characteristics in Jersey, Sahiwal and halfbred bulls. Indian J. Anim. Sci.70, 279-280.
SöderquistL., Jansson L., Larsson K. and Einarsson S. (1991). Sperm morphology and fertility in A.I. bulls. Zentralbl Veterinarmed . 38, 534-543.
SrivastavaS. and Kumar S. (2006). Effect of certain additives on the frezability of crossbred bull semen.Indian J. Anim. Reprod. 27(1), 1-5.
SuttiyovinP., Thwaetes C.J., Sanchez Partida L.G. and Setchell B.P. (1995). Evaluation of a modified sperm penetration test in ram semen. Theriogenology. 44, 29-40.
SwainA.K. and Singh S.V. (2007). Physico-morphological characteristics and their interrelationships in Sahiwal bull semen. Indian Vet. J. 84, 359-362.
TardifA.L., Farrell P.B., Trouern Trend V., Simkin M.E. and Foote R.H. (1998). Use of Hoechst 33342 stain to evaluate live fresh and frozen bull sperm by computer assisted analysis. J. Androl. 19, 201-206.
TesarikJ., Oltras C.M. and Tesfort J. (1990). Effect of human cumulus oophorus on movement characteristics of human capacitated spermatozoa. J. Reprod. Fertil. 88, 665-675.
ThundathilJ., Andrzej T.P., Albert D.B. and Reuben J.M. (2002). Plasma membrane and acrosomal integrity in bovine spermatozoa with knobbed acrosome defect. Theriogenology. 58, 87-102.
TomarN.S., Misra B.S. and Johri C.B. (1966). Seasonal variations in reaction time and semen production and prediction of some semen attributes on initial motility of spermatozoa in Hariana and Murrah bulls. Indian J. Dairy Sci. 19, 87-93.
VeeraiahG., Venugopal K.N. and Rao K.B. (1999). Effects of various levels of glycerol and egg yolk in freezing of Ongole bull semen. Ind. J. Anim. Reprod. 20(2), 149-152.
VerberckmoesS., Van Soom A., De Pauw I., Dewulf J. and De Kruif A. (2002). Migration of bovine spermatozoa in a synthetic medium and its relation to in vivo bull fertility. Theriogenology. 58, 1027-1037.
ZhangB.R., Larsson B., Lundeheim N. and Rodriguez Martinez H. (1998). Sperm characteristics and zona pellucida binding in relation to field fertility of frozen-thawed semen form dairy A.I. bulls. Int. J. Androl. 21, 207-216.
