Single Nucleotide Polymorphisms (SNPs) of GDF9 Gene in Bahmaei and Lak Ghashghaei Sheep Breeds and Its Association with Litter Size
الموضوعات :م. محقق دولت آبادی 1 , ج. حبیبیزاد 2
1 - Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran
2 - Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran
الکلمات المفتاحية: Sheep, polymorphism, litter size, fecundity gene,
ملخص المقالة :
Growth differentiation factor 9 (GDF9) belong to the superfamily of transforming growth factor β that is highly expressed in growing ovarian follicles of oocyte, and it has been strongly related to fecundity traits in sheep. Therefore, the GDF9 gene could serve as a genetic marker for improvement of reproductive performance in sheep. Therefore, the aim of this study was to investigate the fecundity gene of GDF9 in two local sheep breeds, Bahmaei and Lak Ghashghaei, reared at Kuhgiloyeh and Boyer-Ahmad province of Iran. For this purpose, genomic DNA was extracted from 20 Bahmaei and 20 Lak Ghashghaei (10 single birth, 10 twin births in each breed) multiparous ewes. The entire length of exon 1 and 2 in ovine GDF9 gene was amplified using three designed primer pairs, and detection of single nucleotide polymorphisms (SNPs) in the amplified fragments was determined by direct DNA sequencing method. The first and third amplified fragments of ovine GDF9 showed single genotype in two breeds. Sequencing results of the second fragment (P2) discovered 4 SNPs, including 3 SNPs in coding sequence of exon 2 and one novel in intron of GDF9 gene. No significant association was obtained between combined genotypes of identified SNPs with litter size in both sheep breeds. In general, the two sheep breeds were different in their genetic structure with regard to the GDF9 gene. Frequency distributions of identified SNPs in exon 2 GDF9 gene were different in two sheep breeds.
Albarella S., Ciotola F., Selvaggi M., Dario C., Licciardi S., Scopino G., Frate D. and Peretti V. (2015). Analysis of major fecundity genes in autochthonous Laticauda and Bagnolese sheep breeds. Small Rumin. Res. 133, 118-122.
Ali A.S., Ibrahim M.T., Mohammed M.M., Elobied A.A. and Lühken G. (2016). Growth differentiation factor 9 gene variants in Sudanese desert sheep ecotypes. South African J. Anim. Sci. 46, 373-379.
Barzegari A., Atashpaz S., Ghabili K., Nemati Z., Rustaei M. and Azarbaijani R. (2009). Polymorphisms in GDF9 and BMP15 associated with fertility and ovulation rate in Moghani and Ghezel sheep in Iran. Reprod. Domest. Anim. 45, 666-669.
De Castro F.C., Cruz M.H. and Leal C.L. (2016). Role of growth differentiation factor 9 and bone morphogenetic protein 15 in ovarian function and their importance in mammalian female fertility-A review. Asian-Australasian J. Anim. Sci. 29, 1065-1074.
De Soza C.J., McNeilly A.S., Benavides M.V., Melo E.D. and Moraes J.C. (2012). Novel GDF9 polymorphism determining higher ovulation. Pp. 87-91 in Proc. Soc. Reprod. Fertil. Annu. Conf., Edinburgh, United Kingdom.
Eghbalsaied S., Ghaedi K., Shahmoradi S., Pirestani A., Amini H., Saiedi T., Nicol L. and McNeilly A. (2012). Presence of SNPs in GDF9 mRNA of Iranian Afshari sheep. Int. J. Fertil. Steril. 5, 225-230.
Galloway S.M., McNatty K.P., Cambridge L.M., Laitinen M.P., Juengel J.L., Jokiranta T.S., McLaren R.J., Luiro K., Dodds K.G., Montgomery G.W., Beattie A.E., Davis G.H. and Ritvos O. (2000). Mutations in an oocyte-derived growth factor gene (BMP-15) cause increased ovulation rate and infertility in a dosage sensitive manner. Nat. Genet. 25, 279-283.
Hanrahan J.P., Gregan S.M., Mulsant P., Mullen M., Davis G.H., Powell R. and Galloway S.M. (2004). Mutations in the genes for oocyte-derived growth factors GDF-9 and BMP-15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovis aries). Biol. Reprod. 70, 900-909.
Javanmard A., Azadzadeh N. and Esmailizadeh A.K. (2011). Mutations in bone borphogenetic protein 15 andgrowth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds. Vet. Res. Commun. 35, 157-167.
Khaldari M. (2005). Principles of Sheep and Goat Husbandry. Jahad Daneshgahi Publication, Tehran, Iran.
Kasiriyan M.M., Hafezian S.H. and Hassani N. (2011). Genetic polymorphism BMP15 and GDF9 genes in Sangsari sheep ofIran. Int. Genet. Mol. Biol. 3, 31-34.
Lacerda T.S., Caetano A.R., Faco O., de Faria D.A., McManus C.M., Lobo R.N., de Moraes Silva K. and Paiva S.R. (2016). Single marker assisted selection in Brazilian Morada Nova hair sheep community-based breeding program. Small Rumin. Res. 139, 15-19.
Liandris E., Kominakis A., Andreadou M., Kapeoldassi K., Chadio S., Tsiligianni T., Gazouli M. and Ikonomopoulos I. (2012). Associations between single nucleotide polymer phisms of GDF9 and BMP15 genes and litter size in two dairy sheep breeds of Greece. Small Rumin. Resh. 107, 16-21.
Moradband F., Rahimi G. and Gholizadeh M. (2011). Association of polymorphisms in fecundity genes of GDF9, BMP15 and BMP15-1B with litter size in Iranian Baluchi sheep. Asian-Australasian J. Anim. Sci. 24, 1179-1183.
Mullen M.P. and Hanrahan J.P. (2014). Direct evidence on the contribution of a missense mutation in GDF9 to variation in ovulation rate of Finn sheep. PloS one. 9, e95251.
Mulsant P., Lecerf F., Fabre S., Schibler L., Monget P., Lanneluc I., Pisselet C., Riquet J., Monniaux D., Callebaut I., Cribiu E., Thimonier J., Teyssier J., Bodin L., Cognie Y., Chitour N. and Elsen J.M. (2001). Mutation in the bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola ewes. Proc. Natl. Acad. Sci. USA. 98, 5104-5109.
Nicol L., Bishop S.C., Pong-Wong R., Bendixen C., Holm E.L., Rhind S.M. and McNeilly A.S. (2009). Homozygosity for a single base-pair mutation in the oocyte specific GDF9 gene results in sterility in Thoka sheep. Reproduction. 138, 921-933.
Polley S., De S., Brahma B., Mukherjee A., Vinesh P.V., Batabyal S., Arora J.S., Pan S., Samanta A.K., Datta T.K. and Goswami S.L. (2010). Polymorphism of BMPR1B, BMP15 and GDF9 fecundity genes in prolific Garole sheep. Trop. Anim. Health Prod. 42, 985-993.
Roy J., Polley S., De S., Mukherjee A., Batabyal S., Pan S., Brahma B., Datta T.K. and Goswami S.L. (2011). Polymorphism of fecundity genes (FecB, FecX, and FecG) in the Indian Bonpala sheep. Anim. Biotechnol. 22, 151-162.
Sadighi M., Bodensteiner K.J., Beattie A.E. and Galloway S.M. (2002). Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheep chromosome 5. Anim. Genet. 33, 244-245.
SAS Institute. (2012). SAS®/STAT Software, Release 8.1. SAS Institute, Inc., Cary, NC. USA.
Silva B.D., Castro E.A., Souza C.J., Paiva S.R., Sartori R., Franco M.M., Azevedo H.C., Silva T.A., Vieira A.M., Neves J.P. and Melo E.O. (2011). A new polymorphism in the growth and differentiation factor 9 (GDF9) gene is associated with increased ovulation rate and prolificacy in homozygous sheep. Anim. Genet. 42, 89-92.
Souza C.J., MacDougall C., MacDougall C., Campbell B.K., McNeilly A.S. and Baird D.T. (2001). The Boorola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1B (BMPR1B) gene. J. Endocrinol. 169, 1-6.
Souza C.J., McNeilly A.S., Benavides M.V., Melo E.O. and Moraes J.C. (2014). Mutation in the protease cleavage site of GDF9 increases ovulation rate and litter size in heterozygous ewes and causes infertility in homozygous ewes. Anim. Genet. 45, 732-739.
Souza C.J., McNeilly A.S., Benavides M.V., Melo E.O. and Moraes J.C. (2012). Novel GDF9 polymorphism determining higher ovulation rate and litter size in sheep. Available at: https://ainfo.cnptia.embrapa.br/digital/bitstream/item/72796/1/Souza-SRF-2012O005.pdf.
Vage D.I., Husdal M., Matthew P.K., Klemetsdal G. and Boman I.A. (2013). A missense mutation in growth differentiation factor-9 (GDF-9) is strongly associated with litter size in sheep. BMC Genet. 14, 1-9.
Wilson T., Wu X.Y., Juengel J.L., Ross I.K., Lumsden J.M., Lord E.A., Dodds K.G., Walling G.A., McEwan J.C., O’Connell A.R., McNatty K.P. and Montgomery G.W. (2001). Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB Receptor (Alk-6) that is expressed in both oocytes and granulosa cells. Biol. Reprod. 64, 1225-1235.
