The Effect of in ovo Feeding Compared with Dietary Feeding of Betaine on Performance, Immunity and Liver Activity of Broiler Chickens Exposed to High Temperatures
الموضوعات :
A. Maddahian
1
(Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran)
پ. دادور
2
(بخش تحقیقات علوم دامی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان ایلام، سازمان تحقیقات، آموزش و ترویج کشاورزی، ایلام، ایران)
M. Morovat
3
(Young Researchers and Elite Club, Karaj Branch, Islamic Azad University, Karaj, Iran)
M. Shamsaddini Bafti
4
(Department of Pathology and Experimental Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Kerman, Iran)
الکلمات المفتاحية: antibody titer, Immune Organs, cation-anion balance, WBC count,
ملخص المقالة :
This experiment was conducted to investigate the effects of in ovo feeding and dietary feeding of betaine on performance, immunity, liver activity, blood cation-anion balance and bone parameters of chickens exposed to high temperatures. A total of 600 fertile eggs from a broiler breeder strain (Ross 308) were used for in ovo feeding of 0 and 1 g/L betainesolution at 17.5 d of incubation. After hatching, 192 male chicks were transferred to the experimental cages. The diets consisted of two types; one of them without betaine and the other one containing 1 g/kg of betaine. Then chicks were exposed to elevated temperatures 4 ˚C above optimum from 7-28 d of age for 4 h per d and after 28 d they were kept at optimum temperature. The chicks were divided into 4 treatments as a completely randomized 2 × 2 factorial design. There was no effect of in ovo feeding of betaine on performance, immunity parameters, liver activity, blood cation–anion balance and bone parameters. Chickens fed on the diet containing the betaine had the higher feed intake, daily weight gain and final body weight (p <0.05), but not significantly different feed conversion ratio. At 28 d, the weights of spleen and bursa of birds in the treatment groups fed betaine were higher (p <0.01). The betaine-fed group had higher antibody titer against infectious bursal disease (p <0.05), and lower heterophile/lymphocyte ratio, alanine aminotransferase and aspartate aminotransferase enzymes and C-reactive protein (p <0.01). In birds fed on the betaine in the diet, blood cation–anion balance was lower (p <0.01), whereas tibia length (p <0.05), and tibia ash percentage were higher at 28 (p <0.05) and 42 d (p <0.01). These results indicated that dietary feeding of betaine resulted in an improvement in performance, immunity parameters, liver activity, blood cation–anion balance and bone parameters and decreased the adverse effects of the high temperatures.
Alahgholi M., Tabeidian S.A., Toghyani M. and Ale Saheb Fosoul S.S. (2014). Effect of betaine as an osmolyte on broiler chickens exposed to different levels of water salinity. Arch. Tierz. 57, 1-12.
Alfieri R.R., Cavazzoni A., Petronini P.G., Bonelli M.A., Caccamo A.E., Borghetti A.F. and Wheeler K.P. (2002). Compatible osmolytes modulate the response of porcine endothelial cells to hypertonicity and protect them from apoptosis. J. Physiol. 540, 499-508.
Al-Sultan S.I., Abdel-Raheem S.M., Abd-Allah S.M.S. and Edris A.M. (2019). Alleviation of chronic heat stress in broilers by dietary supplementation of novel feed additive combinations. Sloveniian Vet. Res. 56(22), 269-279.
Attia Y.A., Abd El‑Hamid A.E., Abedalla A.A. and Berika M.A. (2016). Laying performance, digestibility and plasma hormones in laying hens exposed to chronic heat stress as affected by betaine, vitamin C, and/or vitamin E supplementation. Spring. Plus. 5(1), 1-12.
Attia Y.A., Hassan R.A., Shehatta M.H. and Abdelhady S.B. (2005). Growth, carcass quality and serum constituents of slow growing chicks as effected by betaine to diets containing 2. Different levels of methionine. Int. J. Poult. Sci. 4, 856-865.
Awaad M.H.H., Elmenawey M.A., Bashandy M.M., Mohamed F.F., Salem H.M., Morsy E.A. and Gossens T. (2018). Heat stress impedance by acidifiers in broiler chickens. Acta Sci. Med. Sci. 2(9), 84-93.
Awad A., Ibrahim A., Fahim H. and Beshara M. (2014). Effect of dietary betaine supplementation on growth performance and carcass traits of domestic ducklings under summer conditions. Egyptian Poult. Sci. J. 34, 1019-1038.
Brees K.W., Raup T.J., Bottje W.G. and Odam T.W., (1989). Physiological responses of heat stress broiler fed nicarbazin. Poult. Sci. 68, 428-434.
Brugh M., Beard C.W. and Wilkes W.J. (1978). The influence of test conditions on Newcastle disease hemagglutination-inhibition titers. Avian Dis. 22, 320-328.
Bruno L.D.G., Furlan R.L., Malheiros E.B. and Macari M. (2000). Influence of early quantitative food restriction on long bone growth at different environmental temperatures in broiler chickens. British Poult. Sci. 41(4), 389-394.
Bruno L.D.G., Luquetti B.C., Furlan R.L. and Macari M. (2007). Influence of early qualitative feed restriction and environmental temperature on long bone development of broiler chickens. J. Therm. Biol. 32, 349-354.
Chand N., Naz S., Maris H., Ullhah Khan R., Khan S. and Qureshi M.S. (2017). Effect of betaine supplementation on the performance and immune response of heat stressed broilers. Pakistan J. Zool. 49(5), 1857-1862.
Dai S.F., Gao F., Zhang W.H., Song S.X., Xu X.L. and Zhou G.H. (2011). Effects of dietary glutamine and gamma-aminobutyric acid on performance, carcass characteristics and serum parameters in broilers under circular heat stress. Anim. Feed Sci. Technol. 168, 51-60.
Deeb N. and Cahaner A. (2001). Genotype –by– environment interaction with broiler of high ambient temperature on dwarf versus normal broilers. Poult. Sci. 80, 541-548.
Dos Santos T.T., DassiS.C., Franco C.R.C., Da Costa C.R.V., Lee S.A. and Da Silva A.V.F. (2019). Influence of fibre and betaine on development of the gastrointestinal tract of broilers between hatch and 14 d of age. Anim. Nutr. 5, 163-173.
Esmailzadeh L., Shivazad M., Sadeghi A.A. and Karimi Torshizi M.A. (2013). The effect of egg powder inclusion in the pre-starter diet on immune response of male broiler chicken. Arch. Tierz. 56, 527-535.
Foye O.T., Uni Z. and Ferket P.R. (2006). Effect of in ovo feeding egg white protein, betahydroxy-beta-methylbutyrate, and carbohydrates on glycogen status and neonatal growth of turkeys. Poult. Sci. 85, 1185-1192.
Gholami J., Qotbi A.A.A., Seidavi A., Meluzzi A., Tavaniello S. and Maiorano G. (2015). Effects of in ovo administration of betaine and choline on hatchability results, growth and carcass characteristics and immune response of broiler chickens. Italian J. Anim. Sci. 14, 3694-3705.
Gudev D., Popova-Ralcheva S., Yanchev I., Moneva P., Petkov E. and Ignatova M. (2011). Effect of betaine on egg performance and some blood constituents in laying hens reared indoor under natural summer temperatures and varying levels of air ammonia. Bulgarian J. Agric. Sci. 17(6), 859-866.
Hamidi H., Jahanian R. and Pourreza J. (2010). Effect of dietary betaine on performance, immunocompetence and gut contents osmolarity of broilers challenged with a mixed coccidial infection. Asian J. Anim. Vet. Adv. 5, 193-201.
Hassan R.A., Attia Y.A. and El-Ganzory E.H. (2005). Growth, carcass quality and serum constituents of slow growing chicks as affected by betaine addition to diets containing different levels of choline. Int. J. Poult. Sci. 4, 840-850.
He S., Zhao S., Dai S., Liu D. and Bokhari S.G. (2015). Effects of dietary betaine on growth performance, fat deposition and serum lipids in broilers subjected to chronic heat stress. J. Anim. Sci. 86, 897-903.
Heidari M., Moeini M. and Nanekarani S. (2013). Effect of Vitamin C, Acetylsalicylic, NaHCO3 and KCL supplementation on the performance of broiler chickens under heat stress condition. Int. J. Agric. Technol. 9, 255-263.
Honarbakhsh S.S., Zaghari M. and Shivazad M. (2007). Can exogenous betaine be an effective osmolyte in broiler chicks under water salinity stress? Asian-Australasian J. Anim. Sci. 20, 1729-1737.
Hu Y., Sun Q., Li X., Wang M., Cai D., Li X. and Zhao R. (2015). In ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks. PloS One. 10(6), e0130786.
Iranian Council of Animal Care. (1995). Guide to the Care and Use of Experimental Animals, vol. 1. IsfahanUniversity of Technology, Isfahan, Iran.
Ismail Z.S.H. and Ahmad E.A.M. (2017). Some physiological responses to dietary betaine levels in broiler chickens. Egyptian Poult. Sci. 37(5), 1249-1259.
Johnston P.A., Liu H., O’Connell T., Phelps P., Bland M., Tyczkowski J., Kemper A., Harding T., Avakian A., Haddad E., Whitfill C., Gildersleeve R. and Ricks C.A. (1997). Applications in in ovo technology. Poult. Sci. 76, 165-178.
Kettunen H., Tiihonen K., Peuranen S., Saarinen M.T. and Remus J.C. (2001). Dietary betaine accumulates in the liver and intestinal tissue and stabilizes the intestinal epithelial structure in healthy and coccidian-infected broiler chicks. Comp. Biochem. Physiol. 130, 759-769.
Khattak F.M., Acamovic T., Sparks N., Naseer Pasha T., Joiya M.H., Hayat Z. and Ali Z. (2012). Comparative efficacy of different supplements used to reduce heat stress in broilers. Pakistan J. Zool. 44, 31-41.
Klasing K.C., Adler K.L., Remus J.C. and Calvert C.C. (2002). Dietary betaine increased intraepithelial lymphocytes in the duodenum of coccidian-infected chicks and increases functional properties of phagocytes. J. Nutr. 132, 2274-2282.
Konca Y. and Kinkpinar F. (2008). Effect of betaine on performance carcass, bone and blood characteristics of broilers during natural summer temperature. J. Anim. Vet. Adv. 7, 930-937.
Kusandi E. and Djulardi A. (2011). Physiological dynamic of broiler at various environmental temperatures. Int. J. Poult. Sci. 10, 19-22.
Mahmoudi M., Azarfar A. and Khosravinia H. (2018). Partial replacement of dietary methionine with betaine and choline in heat-stressed broiler chickens. J. Poult. Sci. 55, 28-37.
Maiorano G., Sobolewska A., Cianciullo D., Walasik K., Elminowska-Wenda G., Sławiska A., Tavaniello S., Zylinska J., Bardowski J. and Bednarczyk M. (2012). Influence of in ovo prebiotic and symbiotic administration on meat quality of broiler chickens. Poult. Sci. 91, 2963-2969.
Matthews J.O. and Southern L.L. (2000). The effect of dietary betaine in Eimeria acervulina infected chicks. Poult. Sci. 79, 60-65.
Morovat M., Chamani M., Zarei A. and Sadeghi A.A. (2016). Dietary but not in ovo feeding of Silybum marianum extract resulted in an improvement in performance, immunity and carcass characteristics and decreased the adverse effects of high temperatures. British Poult. Sci. 57(1), 105-113.
Norouzian H., Alirezaei M., Dezfoulian O. and Taati M. (2108). The effects of post-hatch feeding with betaine on the intestinal development of broiler chickens. Brazilian J. Poul. Sci. 20(3), 403-412.
NRC. (1994). Nutrient Requirements of Poultry, 9th Rev. Ed. National Academy Press, Washington, DC., USA.
Park S.O. and Kim W.K. (2017). Effects of betaine on biological functions in meat-type ducks exposed to heat stress. Poult. Sci. 96, 1212-1218.
Park S.O. and Zammit V.A. (2019). Effect of feed restriction with betaine and ascorbic acid supplementation on caecal bacteria, short chain fatty acid, blood biomarker, duodenal morphology and growth performance of meat ducks under heat stress. Europian Poult. Sci. 83, 47-56.
Pereira R., Menten J.F.M., Longo F.A., Lima M.B., Freitas L.W. and Zavarize K.C. (2018). Sources of betaine as methyl group donors in broiler diets. Iranian J. Appl. Anim. Sci. 8(2), 325-332.
Ratriyanto A., Mosenthin R., Bauer E. and Eklund M. (2009). Metabolic, osmoregulation and nutrition functions of betaine in monogastric animals. Asian-Australasian J. Anim. Sci. 22, 1461-1476.
SahebiAla F., Hassanabadi A. and Golian A. (2019). Effects of dietary supplemental methionine source and betaine replacement on the growth performance and activity of mitochondrial respiratory chain enzymes in normal and heat stressed broiler chickens. J. Anim. Physiol. Anim. Nutr. 103, 87-99.
Sakomura N.K., Barbosa N.A.A., Longo F.A., Silva E.P., Bonato M.A. and Fernandes J.B.K. (2013). Effect of dietary betaine supplementation on the performance, carcass yield, and intestinal morphometrics of broilers submitted to heat stress. Brazilian J. Poult. Sci. 15(2), 105-112.
SAS Institute. (2005). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Shakeri M., Cottrell J.J, Wilkinson S., Ringuet M., Furness J.B. and Dunshea F.R. (2018). Betaine and antioxidants improve growth performance, breast muscle development and ameliorate thermoregulatory responses to cyclic heat exposure in broiler chickens. Animals. 8, 162-171.
Singh A.K., Ghosh T.K., Creswell D.C. and Haldar S. (2015). Effects of supplementation of betaine hydrochloride on physiological performances of broilers exposed to thermal stress. Open Access Anim. Physiol. 7, 111-120.
Slawinska A., Siwek M. and Bednarczyk M. (2014). Effects of synbiotics injected in ovo on regulation of immune-related gene expression in adult chickens. Am. J. Vet. Res. 75, 997-1003.
Smith M.O. and Teeter R.G. (1993). Carbon dioxide, ammonium chloride, potassium chloride and performance of heat stressed broilers. J. Appl. Poult. Res. 2, 61-66.
Sohail M.U., Ijaz A., Yousaf M.S., Ashraf K., Zaneb H., Aleem M. and Rehman H. (2010). Alleviation of cyclic heat stress in broilers by dietary supplementation of mannan-oligosaccharide and Lactobacillus-based probiotic: Dynamics of cortisol, thyroid hormones, cholesterol, C-reactive protein, and humoral immunity. Poult. Sci. 89, 1934-1938.
Tako E., Ferket P.R. and Uni Z. (2004). Effects of in ovo feeding of carbohydrates and beta-hydroxy beta-methylbutyrate on the development of chicken intestine. Poult. Sci. 83(12), 2023- 2028.
Teeter R.G., Remus J.C., Belay T., Mooney M., Virtanen E. and Augustine P. (1999). The effects of betaine on water balance and performance in broilers reared under differing environmental conditions. Pp. 165-171 in Proc. Australian Poult. Sci. Symp., Sydney, Australia.
Tucker L.A. and Remus J. (2001). The effect of betaine on performance, water balance and gut integrity of coccidiosis-infected poultry and its potentioal benefit in AGP-free diets. British Poult. Sci. 42, 108-109.
Uni Z., Ferket P.R., Tako E. and Kedar O. (2005). In ovo feeding improves energy status of late-term chicken embryos. Poult. Sci. 84, 764-770.
Youssef S.F., Abd El-Halim H.A.H., Wahba F.A. and Abd El-Maged M.H. (2017). Effect of in ovo methionine, glutamine, carnitine or betaine injection on hatchability, growth performance and physiological state. Egyptian Poult. Sci. 37(5), 1273-1287.
Yusuf K., Figen K., Selim M. and Erdal Y. (2008). Effects of betaine on performance, carcass bone and blood characteristics of broiler during natural summer temperatures. J. Anim. Vet. Adv. 7(8), 930-937.
Zayed S.M.A. (2012). Physiological studies on turkeys. MS Thesis. MansouraUniv., Mansoura, Egypt.
Zhan X.A., Li J.X., Xu Z.R. and Zhao R.Q. (2006). Effects of methionine and betaine supplementation on growth performance, carcase composition and metabolism of lipids in male broilers. Br. Poult. Sci. 47, 576-580.
Zulkifili I., Mysahra S.A. and Jin L.Z. (2004). Dietary supplementation of betaine (Betafin®) and response to high temperature stress in male broiler chickens. Asian-Australasian J. Anim. Sci. 17(2), 244-249.
