Differences between the Expression of the FSH and LH Genes in the Pituitary Gland of Three Populations of Iranian Native Hens and Hyline W-36
الموضوعات :H. Javaheri Barfourooshi 1 , S.A. Hosseini 2 , A.H. Alizadeh-Ghamsari 3 , A. Yaghobfar 4
1 - Department of Animal Biotechnology, Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
2 - Department of Animal Biotechnology, Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
3 - Department of Animal Biotechnology, Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
4 - Department of Animal Biotechnology, Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
الکلمات المفتاحية: native chicken, Gonadotropin, oviposition, hypophysis, industrial chicken,
ملخص المقالة :
The objective of this study was to compare the expression of pituitary gonadotropin genes between three populations of native birds in Iran and Hyline W-36. One hundred and twenty-eight 37-week-old chickens (Golpayegan, Marandi, Isfahan, and Hyline W-36) were studied for 12 weeks. All birds were reared in the same cage system and under similar nutritional and managerial conditions. Quantitative characteristics such as egg number, egg weight, egg production percentage, egg mass, feed intake, and feed conversion ratio were calculated. At the end of the trial, five hens in each group were slaughtered after weighing, and their heads, livers, ovaries, and oviducts were removed. The gene expression of LH and FSH was determined by reverse transcription-polymerase chain reaction (RT-PCR), after RNA extraction from the pituitary gland. The results showed that Hyline W-36 had the highest egg number, egg weight, percentage of egg production, and egg mass. The feed conversion ratio was the highest in the Marandi, but the lowest was observed in the Hyline W-36. Expression of the FSH gene in Hyline W-36 was higher than in other groups. A positive and significant correlation was observed between the expression of the pituitary gland FSH gene, egg mass, and relative ovarian weight. The difference in production performance of various genetic populations seems to be mainly due to the differential expression of the FSH gene in the pituitary gland. Therefore, for genetic enhancement programs in native chicken, these results may help identify the metabolic pathways of gonadotropins gene expression, their effects on the ovaries, and reproductive activity.
Alders R.G., Dumas S.E., Rukambile E., Magoke G., Maulaga W., Jong J. and Costa R. (2018). Family poultry: Multiple roles, systems, challenges, and options for sustainable contributions to household nutrition security through a planetary health lens. Matern. Child Nutr. 3, 12668-12672.
Arab Abusaadi M., Roghani I. and Hasanzadeh B. (2006). The effect of different energy levels and dietary energy to protein ratio on the performance of native laying hens in Fars province in the second stage of laying. J. Sci. Technol. Agric. Nat. Res. 10, 457-469.
Bain M.M., Nys N. and Dunn I.C. (2015). Increasing persistency in lay and stabilizing egg quality in longer laying cycles- What are the challenges? British Poult. Sci. 57, 330-338.
Bedecarrats G.Y. (2015). Control of the reproductive axis: balancing act between stimulatory and inhibitory inputs. Poult. Sci. 94, 810-815.
Bounds M. and Zinyemba O. (2018). Poultry farming: Lessening poverty in rural areas. South African J. Agric. Ext. 46, 59-70.
Dana N. (2011). Breeding programs for indigenous chicken in Ethiopia: Analysis of diversity in production systems and chicken populations. Ph D. Thesis. Wageningen Univ., The Netherlands.
Dessie T., Taye T., Dana N., Ayalew W. and Hanotte O. (2011). The current state of knowledge on phenotypic characteristics of indigenous chickens in the tropics. World's Poult. Sci. J. 67, 507-516.
FASS. (2010). Guide for Care and Use of Agricultural Animals in Research and Teaching. Federation of Animal Science Societies, Champaign, Illinois, USA.
Gheisari A.A., Maghsoudinejad G. and Azarbayejani A. (2016). Evaluation of laying performance and egg qualitative characteristics of indigenous hens reared in rural areas of Isfahan province. Iranian J. Appl. Anim. Sci. 6, 957-962.
Grobbelaar J.A.N., Sutherland B. and Molalakgotla N.M. (2010). Egg production potentials of certain indigenous chicken breeds from South Africa. Anim. Genet. Resour. 46, 25-32.
Hashemi M.R. (2012). Effect of feeding different levels of canola seed on performance, Egg characteristics and fatty acid content in yolk of Fars native chicken. Pp. 1084-1088 in Proc. 5th Anim. Sci. Congr., Isfahan, Iran.
Haunshi S. and Rajkumar U. (2020). Native chicken production in India: present status and challenges. Livest. Res. Rural Dev. 32, 1-11.
Haunshi S., Doley S. and Shakuntala I. (2009). Production performance of indigenous chicken of northeastern region and improved varieties developed for backyard farming. Indian J. Anim. Sci. 79, 901-905.
Hesabi Nameghi A. (2012). Effect of different levels of crude protein in native hens’ performance of Khorasan station. Anim. Sci. J. (Pajouhesh and Sazandegi). 95, 13-20.
Hu S., Duggavathi R. and Zadworny D. (2017). Regulatory mechanisms underlying the expression of prolactin receptor in chicken granulosa cells. PLoS One. 12, e0170409.
Johnson A.L. (2015). Reproduction in the females. Pp. 635-665 in Sturkie's Avian Physiology. C.G. Scanes, Elsevier, New York.
Lei M.M., Wu S.Q., Li X.W., Wang C.L., Chen Z. and Shi Z.D. (2014). Leptin receptor signaling inhibits ovarian follicle development and egg-laying in chicken hens. Reprod. Boil. Endocrinol. 12, 25-36.
Lovell T.M., Knight P.G. and Gladwell R.T. (2005). Variation in pituitary expression of mRNAs encoding the putative inhibin co-receptor (beta glycan) and type-I and type-II activin receptors during the chicken ovulatory cycle. J. Endocrinol. 186, 447-455.
Mupeta B., Wood J., Mandonga F. and Mhlanga J. (2000). A comparison of the performance of free-range chickens, under improved feed management, with the performance of hybrid chickens in tropical Zimbabwe. Pp 26-28 in Proc. 3rd Workshop on Livest. Prod. Prog. Proj., Ingwe lodge and ICRISAT, Matobo, Zimbabwe.
Musgrove M.T. (2011). Microbiology and safety of table eggs. Pages 3-33 in Improving the Safety and Quality of Eggs and Egg Products. F. Van Immerseel, Y. Nys and M. Bain, Eds. Woodhead Publishing, United Kingdom.
Nguyen Van D., Moula N., Moyse E., Duc L.D., Dinh T. V. and Farnir F. (2020). Productive performance and egg and meat quality of two indigenous poultry breeds in Vietnam, Ho and Dong Tao, fed on commercial feed. Animals. 10, 408-418.
Ocon-Grove O.M., Maddineni S., Hendricks III G.L., Elkin R.G., Proudman J.A. and Ramachandran R. (2007). Pituitary progesterone receptor expression and plasma gonadotrophin concentrations in the reproductively dysfunctional mutant restricted ovulatory chicken. Domest. Anim. Endocrinol. 32, 201-215.
Okoro V.M.O., Ravhuhali K.E., Mapholi T.H., Mbajiorgu E.F. and Mbajiorgu C.A. (2017). Effect of age on production characteristics of Boschveld indigenous chickens of South Africa reared intensively. South African J. Anim. Sci. 47, 157-167.
Olarotimi O.J. (2021). Performance, egg characteristics, hematological and serum biochemical profiles of laying hens fed varying levels of cerium chloride and oxide. Iranian J. Appl. Anim. Sci. 11(1), 169-178.
Onagbesan O.M., Metayer S., Tona K., Williams J., Decuypere E. and Bruggeman V. (2006). Effects of genotype and feed allowance on plasma luteinizing hormones, follicle-stimulating hormones, progesterone, estradiol levels, follicle differentiation, and egg production rates of broiler breeder hens. Poult. Sci. 85, 1245-1258.
Pourreza J. (2003). Chicken Nutrition. Amir Kabir Publication, Isfahan, Iran.
Ramlah A.H. (1996). Performance of village chicken in Malaysia. World's Poult. Sci. 52, 75-79.
Rexroad C., Vallet J., Matukumalli L.K., Reecy J., Bickhart D., Blackburn H., Boggess M., Cheng H., Clutter A., Cockett N., Ernst C., Fulton J.E., Liu J., Lunney J., Neibergs H., Purcell C., Smith T.P.L., Sonstegard T., Taylor J., Telugu B., Eenennaam A.V., Tassell C.P.V. and Wells K. (2019). Genome to phenome: Improving animal health, production, and well-being-A new USDA blueprint for animal genome research 2018-2027. Front. Genet. 10, 327-335.
Richards M.P. and Proszkowiec-Weglarz M. (2007). Mechanisms regulating feed intake, energy expenditure, and body weight in poultry. Poult. Sci. 86, 1478-1490.
Ritchie M. (2014). Neuroanatomy and physiology of the avian hypothalamic/pituitary axis: clinical aspects. Vet. Clin. Exot. Anim. Pract. 17, 13-22.
SAS Institute. (2004). SAS®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.
Sutherland D.A.T., Honaker C.F. and Siegel P.B. (2017). Dual functioning ovaries and atresia in chickens. Is it a coincidence? Poult. Sci. 96, 3763-3767.
Van der Klein S.A.S., Zuidhof M.J. and Bedecarrats G.Y. (2020). Diurnal and seasonal dynamics affecting egg production in meat chickens: A review of mechanisms associated with reproductive dysregulation. Anim. Reprod. Sci. 213, 106257-106266.
Yao Y., Yang Y.Z., Gu T.T., Cao Z.F., Zhao W.M., Qin H.R., Xu Q. and Chen G.H. (2019). Comparison of the broody behavior characteristics of different breeds of geese. Poult. Sci. 98, 5226-5233.
Zhang Z., Lai S., Wang Y., Li L., Yin H., Wang Y., Zhao X., Li D., Yang M. and Zhu Q. (2017). Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of laying hen. PloS One. 12, e0179019.
