Effect of Adding Different Levels of Folic Acid to the Culture Medium on Developmental Competence of Bovine Oocytes
الموضوعات :H. Baghshahi 1 , S. زین الدینی 2 , A. Zare Shahneh 3 , S.E. Khanian 4 , A.R. Yousefi 5 , A. Goodarzi 6
1 - Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 - Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 - Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 - Department of Biotechnology, Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
5 - Department of Pathology and Experimental Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
6 - Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
الکلمات المفتاحية: Homocysteine, bovine, IVF, IVM, folate,
ملخص المقالة :
This study aimed to investigate the effects of adding different levels of folic acid (FoA) in culture medium on bovine embryo developmental competence, reactive oxygen species (ROS) content, and expression of DNA methylation genes. In this study, different levels of FoA including 0, 10, 100, and 1000 ng/mL were added to maturation medium, and then the matured oocytes were subjected to in vitro fertilization (IVF) and in vitro culture (IVC). To determine the intracellular ROS activity in oocytes, a 20, 70-dichlorofluorescein light assay was used. RNA was extracted from ovaries, denuded mature oocytes and blastocysts using a commercial kit to determine relative expression of DNA methyltransferase 1 (Dnmt1), Dnmt3a, and Dnmt3b. The addition of FoA decreased (p <0.05) oocyte ROS content in all the treated groups compared to the control group; meanwhile, the lowest level was noted in FoA100 group (p <0.05). The fertilization rate was improved (p <0.05) in FoA10 and FoA100 compared to the control group. Transcript abundance of Dnmt1 in the matured oocyte was increased in FoA10 and FoA100 compared to the control (p <0.05), but the highest level was noted in FoA100. Folic acid enhanced the expression of Dnmt3a and decreased that of Dnmt3b in bovine oocytes (p <0.05). Transcript abundance of Dnmt3a and Dnmt3b in the FoA100 blastocysts indicated an increase and decrease, respectively relative to the control group (p <0.05). These findings showed that the addition of 100 ng/mL of FoA to the oocyte maturation culture medium has a beneficial effect on the fertilization rate and developmental competence of produced zygote.
Abazarikia A.H., Zhandi M., Shakeri M., Towhidi A. and Yousefi A.R. (2020). In vitro supplementation of trans-10, cis-12 conjugated linoleic acid ameliorated deleterious effect of heat stress on bovine oocyte developmental competence. Theriogenology. 142, 296-302.
Baghshahi H., Zeinoaldini S., Zare Shahneh A. and Esmaeilkhanian S. (2019). Effect of folic acid addition to the medium on in vitro maturation of bovine oocytes. J. Anim. Prod. 21(3), 393-400.
Balboula A.Z., Yamanaka K.I., Sakatani M., Kawahara M., Hegab AE-R., Zaabel S. and Takahashi M. (2013). Cathepsin B activity has a crucial role in the developmental competence of bovine COCs exposed to heat-shock during in vitro maturation. Reproduction. 146(4), 407-417.
Bardaweel S.K., Gul M., Alzweiri M., Ishaqat A., ALSalamat H.A. and Bashatwah R.M. (2018). Reactive oxygen species: The dual role in physiological and pathological conditions of the human body. Eurasian J. Med. 50(3), 193-201.
Camargo L.S.A., Viana J.H.M., Sá W.F., Ferreira A.M., Ramos A.A. and Vale Filho V.R. (2018). Factors influencing in vitro embryo production. Anim. Reprod. 3(1), 19-28.
Caraviello D., Weigel K., Fricke P., Wiltbank M., Florent M., Cook N., Nordlund K.V., Zwald N.R. and Rawson CL. (2006). Survey of management practices on reproductive performance of dairy cattle on large US commercial farms. J. Dairy Sci. 89(12), 4723-4735.
Eagle H. (1955). The specific amino acid requirements of a mammalian cell (strain L) in tissue culture. J. Biol. Chem. 214(2), 839-852.
Ebisch I., Thomas C., Peters W., Braat D. and Steegers-Theunissen R. (2007). The importance of folate, zinc and antioxidants in the pathogenesis and prevention of subfertility. Hum. Reprod. Update. 13(2), 163-174.
Fang Y., Zhang X., Zhang J., Zhong R. and Zhou D. (2016). Global DNA methylation and related mRNA profiles in sheep oocytes and early embryos derived from pre-pubertal and adult donors. Anim. Reprod. Sci. 164, 144-151.
Farin P., Crosier A. and Farin C. (2001). Influence of in vitro systems on embryo survival and fetal development in cattle. Theriogenology. 55(1), 151-170.
Gennari Verruma C., Mioranza A., Miranda Furtado C.L., Vila R.A., Ramos E.S. and Lõbo R.B. (2016). In vitro production of bovine embryos: partial replacement of fetal calf serum by bovine serum albumin on in vitro culture and addition of folic acid on oocytes maturation. Pp. 334-337 in Proc. 30th Ann. Meet. Brazilian Embryo Technol. Soc., Jaboticabal, Brazil.
Guimarães A.L.S. and Dode, M.A.N. (2016). Effect of various concentrations of folic acid on in vitro culture of bovine embryos. Pp. 307-311 in Proc. 30th Ann. Meet. Brazilian Embryo Technol. Soc., Jaboticabal, Brazil.
Hirasawa R., Chiba H., Kaneda M., Tajima S., Li E., Jaenisch R. and Sasaki H. (2008). Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development. Genes Dev. 22(12), 1607-1616.
Huang X., Gao S., Xia W., Hou S. and Wu K. (2013). Folic acid facilitates in vitro maturation of mouse and Xenopus laevis oocytes. British J. Nutr. 109(8), 1389-1395.
Hussein T.S., Sutton-McDowall M.L., Gilchrist R.B. and Thompson J.G. (2011). Temporal effects of exogenous oocyte-secreted factors on bovine oocyte developmental competence during IVM. Reprod. Fertil. Dev. 23(4), 576-584.
Joshi R., Adhikari S., Patro B., Chattopadhyay S. and Mukherjee T. (2001). Free radical scavenging behavior of folic acid: Evidence for possible antioxidant activity. Free Radic. Biol. Med. 30(12), 1390-1399.
Kato Y., Kaneda M., Hata K., Kumaki K., Hisano M., Kohara Y., Okano M., Li E., Nozaki M. and Sasaki H. (2007). Role of the Dnmt3 family in de novo methylation of imprinted and repetitive sequences during male germ cell development in the mouse. Hum. Mol. Genet. 16(19), 2272-2280.
Kim E.S., Seo J.S., Eum J.H., Lim J.E., Kim D.H., Yoon T.K. and Lee D.R. (2008). The effect of folic acid on in vitro maturation and subsequent embryonic development of porcine immature oocytes. Mol. Reprod. Dev. 76(2), 120-121.
Koyama H., Ikeda S., Sugimoto M. and Kume S. (2012). Effects of folic acid on the development and oxidative stress of mouse embryos exposed to heat stress. Reprod. Domest. Anim. 47(6), 921-927.
Kwong W.Y., Adamiak S.J., Gwynn A., Singh R. and Sinclair K.D. (2010). Endogenous folates and single-carbon metabolism in the ovarian follicle, oocyte and pre-implantation embryo. Reproduction. 139(4), 705-712.
Lee D., Kim E., Lim J., Seo J., Kim A. and Won H. (2008). The effect of supplemented folic acid in the maturation medium on the maturation of porcine immature oocytes and subsequent embryonic development. Fertil. Steril. 90, 333-334.
Livak K.J. and Schmittgen T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods. 25(4), 402-408.
Merton J., De Roos A., Mullaart E., De Ruigh L., Kaal L., Vos P. and Dieleman S. (2003). Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry. Theriogenology. 59(2), 651-674.
Nahak G., Suar M. and Sahu R.K. (2014). Antioxidant potential and nutritional values of vegetables: A review. Res. J. Med. Plant. 8(2), 50-81.
Okano M., Xie S. and Li E. (1998). Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases. Nat. Genet. 19(3), 219-225.
Ortega M.S., Kurian J.J., McKenna R. and Hansen P.J. (2017). Characteristics of candidate genes associated with embryonic development in the cow: Evidence for a role for WBP1 in development to the blastocyst stage. PlOS One. 12(5), e0178041.
Pasquale L.R., Borrás T., Fingert J.H., Wiggs J.L. and Ritch R. (2016). Exfoliation syndrome: Assembling the puzzle pieces. Acta Ophthalmol. 94(6), 505-512.
Pellegrino C., Morotti F., Untura R., Pontes J., Pellegrino M., Campolina J., Seneda M.M., Barbosa F.A. and Henry M. (2016). Use of sexed sorted semen for fixed-time artificial insemination or fixed-time embryo transfer of in vitro–produced embryos in cattle. Theriogenology. 86(3), 888-893.
Ratnam S., Mertineit C., Ding F., Howell C.Y., Clarke H.J., Bestor T.H., Chaillet J.R. and Trasler J.M. (2002). Dynamics of Dnmt1 methyltransferase expression and intracellular localization during oogenesis and preimplantation development. Dev. Biol. 245(2), 304-314.
Saini S., Sharma V., Kumar A., Thakur A., Bajwa K. and Malakar D. (2018). Effect of folic acid supplementation on in vitro maturation of oocytes and folate cycle. Reprod. Fertil. Dev. 30(1), 224-225.
SAS Institute. (2004). SAS®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.
Sato D., Sakurai K., Monji Y., Kuwayama T. and Iwata H. (2013). Supplementation of maturation medium with folic acid affects DNA methylation of porcine oocytes and histone acetylation of early developmental stage embryos. J. Mamm. Ova. Res. 30(3), 109-116.
Schulz K.N. and Harrison M.M. (2019). Mechanisms regulating zygotic genome activation. Nat. Rev. Genet. 20(4), 221-234.
Song Z., Min L., Pan Q., Shi Q. and Shen W. (2009). Maternal imprinting during mouse oocyte growth in vivo and in vitro. Biochem. Biophys. Res. Commun. 387(4), 800-805.
Stanger O. and Wonisch W. (2012). Water Soluble Vitamins. Springer-Verlag Berlin Heidelberg Publisher, Germany.
Steele W., Allegrucci C., Singh R., Lucas E., Priddle H., Denning C., Sinclair K. and Young L. (2005). Human embryonic stem cell methyl cycle enzyme expression: Modelling epigenetic programming in assisted reproduction? Reprod. Biomed. Online. 10(6), 755-766.
Stover P.J. (2009). One-carbon metabolism–genome interactions in folate-associated pathologies. J. Nutr. 139(12), 2402-2405.
Tawatao K.M., Manaois I., Ocampo M. and Ocampo M.B. (2015). Folic acid supplementation for bovine oocyte maturation and fertilization in vitro. J. Agric. Technol. 11(8), 2401-2409.
Uysal F., Akkoyunlu G. and Ozturk S. (2015). Dynamic expression of DNA methyltransferases (DNMTs) in oocytes and early embryos. Biochimie. 116, 103-113.
Van den Veyver I.B. (2002). Genetic effects of methylation diets. Annu. Rev. Nutr. 22(1), 255-282
Ventura-Juncá P., Irarrázaval I., Rolle A.J., Gutiérrez J.I., Moreno R.D. and Santos M.J. (2015). In vitro fertilization (IVF) in mammals: Epigenetic and developmental alterations. Scientific and bioethical implications for IVF in humans. Biol. Res. 48(1), 68-76.
Walsh S., Williams E. and Evans A. (2011). A review of the causes of poor fertility in high milk producing dairy cows. Anim. Reprod. Sci. 123(3), 127-138.
Whidden L., Martel J., Rahimi S., Chaillet J.R., Chan D. and Trasler J.M. (2016). Compromised oocyte quality and assisted reproduction contribute to sex-specific effects on offspring outcomes and epigenetic patterning. Hum. Mol. Genet. 25(21), 4649-4660.
Young L.E., Fernandes K., McEvoy T.G., Butterwith S.C., Gutierrez C.G., Carolan C., Broadbent P.J., Robinson J.J., Wilmut I. and Sinclair K.D. (2001). Epigenetic change in IGF2R is associated with fetal overgrowth after sheep embryo culture. Nat. Genet. 27(2), 153-161.
Yousefi A.R., Kohram H., Zare Shahneh A., Zamiri M.J., Dirandeh E., Kazemi Khoozani M., Dadashpour-Davachi N., Zhandi M. and Folladi-Nashta A.A. (2019). Effect of oral administration of pioglitazone on follicular dynamics in Holstein dairy cows. Livest. Sci. 224, 50-56.
