Association of Stearoyl-CoA Desaturase Expression with Cattle Milk Characteristics
الموضوعات :M.R. Ahsani 1 , M.R. Mohammadabadi 2 , V. Buchkovska 3 , Y. Ievstafiieva 4 , D.M. Kucher 5 , O.A. Kochuk-Yashchenko 6 , O.I. Babenko 7 , R.V. Stavetska 8 , V.P. Oleshko 9 , O. Kalashnyk 10
1 - Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran
2 - Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran
3 - State Agrarian and Engineering University in Podilia, Kamianets-Podilskyi, Ukraine
4 - State Agrarian and Engineering University in Podilia, Kamianets-Podilskyi, Ukraine
5 - Department of Breeding, Animal Genetics and Biotechnology, Polissia National University, Ukraine
6 - Department of Breeding, Animal Genetics and Biotechnology, Polissia National University, Ukraine
7 - Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine
8 - Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine
9 - Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine
10 - Sumy National Agrarian University, Sumy, Ukraine
الکلمات المفتاحية: cattle, Canola, soybean, SCD gene expression,
ملخص المقالة :
Stearoyl-CoA desaturase (SCD) enzyme plays an important role in the metabolism of the lipids, thus the goal of this study was to investigate the influence of canola and soybean oilseeds on gene expression of SCD in adipose tissue, composition and yield of milk, fatty acid profile in Iranian Holstein cattle. Animals (n=20) were randomly selected to test experimental diets. Fatty acid composition was determined. After isolation of total RNA, cDNA was synthesized. Reverse transcription polymerase chain reaction (RT-PCR) was used to amplify SCD and GAPDH. For analyzing the real-time PCR results, LinRegPCR, REST and SPSS softwares was employed. The animals fed canola seed in comparison animals fed soybean seed showed higher gene expression. The milk production, fat percentage, 4% fat corrected milk, body condition score and milk urea nitrogen showed a significant difference between two groups. The amount of a number of fatty acids extracted from adipose tissue including C18:3t, C18:0 and C16:1 in animals fed two different diets was variable and their amount was significantly different. SCD gene expression was not significantly different between animals fed two diets (canola and soybean). This may be due to the similarity of the fatty acid composition of the two compounds and their nutrient balance. Since canola seed are higher in fat and protein than soybean seed, it can be a good substitute for soybean seed in the diet of dairy cows. In addition, canola seed, with the effect of nutrition on the composition of milk fatty acids can be used to improve milk.
Al-Hasani H. and Joost H.G. (2005). Nutrition-/diet-induced changes in gene expression in white adipose tissue. Best Pract. Res. Clinl. Endocrinol. Metab. 19, 589-603.
Alim M.A., Fan Y.P., Wu X.P., Xie Y., Zhang Y., Zhang S.L., Sun D.X., Zhang Y., Zhang Q., Liu L. and Guo G. (2012). Genetic effects of staeroyl-coenzyme A desaturase (SCD) polymorphism on milk production traits in the Chinese dairy population. Mol. Biol. Rep. 39, 8733-8740.
Alinaghizadeh R., Mohammad Abadi M.R. and Moradnasab Badrabadi S. (2007). Kappa-casein gene study in Iranian Sistani cattle breed (Bos indicus) using PCR-RFLP. Pakistan J. Biol. Sci. 10, 4291-4294.
Barazandeh A., Mohammadabadi M.R., Ghaderi M. and Nezamabadipour H. (2016). Predicting CpG islands and their relationship with genomic feature in cattle by Hidden Markov Model algorithm. Iranian J. Appl. Anim. Sci. 6, 571-579.
Beauchemin K.A., Mcginn S.M., Benchaar C. and Holtshausen C. (2009). Crushed sunflower, flax, or canola seeds in lactating dairy cow diets: Effects on methane production, rumen fermentation, and milk production. J. Dairy Sci. 92, 2118-2127.
Brooks M.A., Choi C.W., Lunt D.K., Kawachi H. and Smith S.B. (2011). Subcutaneous and intramuscular adipose tissue stearoyl-coenzyme a desaturase gene expression and fatty acid composition in calf- and yearling-fed Angus steers. J. Anim. Sci. 89, 2556-2570.
Burlingame B., Nishida C., Uauy R. and Weisell R. (2009). Fats and fatty acids in human nutrition. Ann. Nutr. Metab. 55, 5-7.
Conte G., Jeronimo E., Serra A., Bessa R.J.B. and Mele M. (2012). Effect of dietary polyunsaturated fatty acids on Stearoyl CoA-Desaturase gene expression in intramuscular lipids of lamb. Italian J. Anim. Sci. 11(79), 453-458.
Corl B.A., Baumgard L.H., Dwyer D.A., Griinari J.M., Phillips B.S. and Bauman D.E. (2001). The role of Δ9-desaturase in the production of cis-9, trans-11 CLA. J. Nutr Biochem. 12, 622-630.
Dobrzyn A. and Dobrzyn P. (2006). Stearoyl-CoA desaturase-a new player in skeletal muscle metabolism regulation. J. Physiol. Pharmacol. 57, 31-42.
Dridi S., Taouis M., Gertler A., Decuypere E. and Buyse J. (2007). The regulation of stearoyl-CoA desaturase gene expression is tissue specific in chickens. J. Endocrinol. 192, 229-236.
Ebrahimi Z., Mohammadabadi M.R., Esmailizadeh A.K., Khezri A. and Najmi Noori A. (2015a). Association of PIT1 gene with milk fat percentage in Holstein cattle. Iranian J. Appl. Anim. Sci. 5, 575-582.
Ebrahimi Z., Mohammadabadi M.R., Esmailizadeh A.K., Khezri A. and Najmi Noori A. (2015b). Association of PIT1 gene and milk protein percentage in Holstein cattle. J. Livest. Sci. Technol. 3, 41-49.
Endo Y., Fu Z. and Abe K. (2002). Dietary protein quantity and quality effect rat hepatic gene expression. J. Nutr. 132, 3632-3637.
Esmaeili H.R., Hozhabri F., Moeini M.M. and Hajarian H. (2016). Effects of replacing roasted soybean seeds with roasted canola seeds in diet of lactating cows on milk production and milk fatty acid composition. J. Rumin. Res. 4, 167-187.
Gamarra D., Aldai N., Arakawa A., Barron L.J.R., López-Oceja A., de Pancorbo M.M. and Taniguchi M. (2018). Distinct correlations between lipogenic gene expression and fatty acid composition of subcutaneous fat among cattle breeds. BMC Vet. Res. 14, 167-176.
Ghasemi M., Baghizadeh A. and Abadi M.R.M. (2010). Determination of genetic polymorphism in Kerman Holstein and Jersey cattle population using ISSR markers. Australian J. Basic Appl. Sci. 4, 5758-5760.
Haro D., Marrero P.F. and Relat J. (2019). Nutritional regulation of gene expression: Carbohydrate-, fat- and amino acid-dependent modulation of transcriptional activity. Int. J. Mol. Sci. 20, 1386-1394.
Herdman A., Nuernberg K., Martin J., Nuerberg G. and Doran O. (2010). Effect of dietary fatty acids on expression of lipogenic enzymes and fatty acid profile in tissues of bulls. Animals. 4, 755-762.
Hosseini F., Mousavi A. and Danesh M. (2012). Canola and soybean meal replacement effect on some production traits in Holstein dairy cow parturition. Res. J. Anim. Sci. 4, 39-45.
Jacob F. and Monod J. (1961). Genetic regulatory mechanisms in the synthesis of proteins. J. Mol. Biol. 3, 318-356.
Johnson K.A., Kincaid R.L., Westberg H.H., Gaskins C.T., Lamb B.K. and Cronrath J.D. (2002). The effect of oilseeds in diets of lactating cows on milk production and methane emissions. J. Dairy Sci. 85, 1509-1515.
Joseph S.J., Scott L.P., Enrique P., Romdhane R. and Susan K.D. (2010). Omega-6 fat supplementation alters lipogenic gene expression in bovine subcutaneous adipose tissue. Gene Regul. Syst. Biol. 4, 91-101.
Jump D.B. and Clarke S.D. (1999). Regulation of gene expression by dietary fat. Ann. Rev. Nutr. 19, 63-90.
Kharrati Koopaei H., Mohammad Abadi M.R., Ansari Mahyari S., Tarang A.R., Potki P. and Esmailizadeh A.K. (2012). Effect of DGAT1 variants on milk composition traits in Iranian Holstein cattle population. Anim. Sci. Pap. Rep. 30, 231-240.
Lanza M., Fabro C., Scerra M., Bella M., Pagano R., Brogna D.M.R. and Pennisi P. (2011). Lamb meat quality and intramuscular fatty acid composition as affected by concentrates including different legume seeds. Italian J. Anim. Sci. 10, 87-94.
Machmuller A., Ossowski D.A. and Kreuzer M. (2000). Comparative evaluation of the effects of coconut oil, oilseeds and crystalline fat on methane release, digestion and energy balance in lambs. Anim. Feed Sci. Technol. 85, 41-60.
Mannen H. (2012). Genes associated with fatty acid composition of beef. Food Sci. Technol. Res. 18, 1-6.
Miyazaki M. and Ntambi J.M. (2003). Role of stearoyl-coenzyme a desaturase in lipid metabolism. Prostag. Leukotr. Ess. Fatty Acids. 68, 113-121.
Mohammadabadi M.R., Soflaei M., Mostafavi H. and Honarmand M. (2011). Using PCR for early diagnosis of bovine leukemia virus infection in some native cattle. Genet. Mol. Res. 10, 2658-2663.
Ntambi J.M. and Miyazaki M. (2004). Regulation of stearoyl CoA desaturases and role in metabolism. Prog. Lipid Res. 43, 91-104.
Pasandideh M., Mohammadabadi M.R., Esmailizadeh A.K. and Tarang A. (2015). Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle. Czech J. Anim. Sci. 60, 97-104.
Ritzenthaler K.L., McGuire M.K., Falen R., Shultz T.D., Dasgupta N. and McGuire M.A. (2001). Estimation of conjugated linoleic acid intake by written dietary assessment methodologies underestimates actual intake evaluated by food duplicate methodology. J. Nutr. 131, 1548-1554.
Ruzina M.N., Shtyfurko T.A., Mohammadabadi M.R., Gendzhieva O.B., Tsedev T. and Sulimova G.E. (2010). Polymorphism of the BoLA-DRB3 gene in the Mongolian, Kalmyk, and Yakut cattle breeds. Russian J. Genet. 46, 456-463.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.
Taniguchi M., Utsugi T., Oyama K., Mannen H., Kobayashi M., Tanabe Y., Ogino A. and Tsuji S. (2004). Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle. Mamm. Genome. 15, 142-148.
Waters S.M., Kelly J.P., O’Boyle P., Moloney A.P. and Kenny D.A. (2009). Effect of level and duration of dietary n-3 polyunsaturated fatty acid supplementation on the transcriptional regulation of delta 9- desaturase in muscle of beef cattle. J. Anim. Sci. 87, 244-252.
Zheng Y., Prouty S.M., Harmon A., Sundberg J.P., Stenn K.S. and Parimoo S. (2001). SCD-3 a novel gene of the stearoyl-CoA desaturase family with restricted expression in skin. Genome 71, 182-191.
