Evaluation of Lactoferrin, CD74, GLYCAM1, and FCER1G Genes Expression on Mammary Epithelial Tissue between Holstein Cows with Clinical Mastitis and Healthy Cows
الموضوعات :
L. Nabinezhad
1
(Department of Basic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
F. Moradian
2
(Department of Basic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
A. Farhadi
3
(Department of Basic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
الکلمات المفتاحية: Mastitis, <i>CD74</i> gene, <i>FCER1G</i> gene, <i>GLYCAM1</i> gene, <i>Lf</i> gene,
ملخص المقالة :
Mastitis is one of the most economically significant diseases in the dairy cattle industry, which increases treatment costs and reduces milk production, genetic advancement of the herd, and reduced milk quality. In the present study, the expression level of some influential genes involved in the immune system as well as disease including lactoferrin, CD74, glycosylation dependent cell adhesion molecule 1, and FCER1G genes were investigated. For this purpose, the breast tissue was separated from 10 cows with clinical mastitis and 10 healthy cows and total RNA was extracted from epithelial cells of mammary tissue of healthy and diseased cows using a kit. Then cDNA was synthesized using kits and the relative expression of the genes was measured using the real-time PCR technique. The 18S rRNA gene was used as a housekeeping gene. The relative expression of genes was measured by the 2-ΔΔct method and statistical differences between healthy and patient groups were examined by t-test. The results showed that the expression levels of Lf, CD74, and FCER1G genes in the group of cows with clinical mastitis were 2.5 (P<0.0003), 6 (P<0.0004), and 2 (P<0.002) times higher than healthy cows, respectively. In contrast, the expression level of the GLYCAM1 gene was significantly lower (4 times) in the group of cows with clinical mastitis comparing healthy cows (P<0.0074). Given the important role of these genes in immune responses, it is important to study them as candidate genes for resistance to mastitis disease.
Abebe R., Hatiya H., Abera M., Megersa B. and Asmare K. (2016). Bovine mastitis: Prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia. BMC Vet. Res. 12(1), 1-11.
Asselstine V., Miglior F., Suarez-Vega A., Fonseca P.A.S., Mallard B., Karrow N. and Cánovas A. (2019). Genetic mechanisms regulating the host response during mastitis. J. Dairy Sci. 102(10), 9043-9059.
Behl J.D., Verma N.K., Tyagi N., Mishra P., Behl R. and Joshi B.K. (2012). The major histocompatibility complex in bovines: A review. ISRN Vet. Sci. 2012, 872710.
Beswick E.J. and Reyes V.E. (2009). CD74 in antigen presentation, inflammation, and cancers of the gastrointestinal tract. World J. Gastroenterol. 15(23), 2855-2861.
Bruckmaier R.M. (2005). Gene expression of factors related to the immune reaction in response to intramammary Escherichia coli lipopolysaccharide challenge. J. Dairy Res. 72, 120-124.
Chaneton L., Tirante L., Maito J., Chaves J. and Bussmann L.E. (2008). Relationship between milk lactoferrin and etiological agent in the mastitic bovine mammary gland. J. Dairy Sci. 91, 1865-1873.
Chopra A., Gupta I.D., Verma A. and Vohra V. (2014). Detection of allelic variants in lactoferrin gene promoter using created restriction site PCR-RFLP and its association with mastitis. Indian J. Anim. Sci. 84, 1068-1070.
Chopra A., Gupta I.D., Verma A., Chakravarty A.K. and Vohra V. (2015). Lactoferrin gene promoter variants and their association with clinical and subclinical mastitis in indigenous and crossbred cattle. Pol. J. Vet. Sci. 18(3), 465-471.
Dowbenko D., Kikuta A., Fennie C., Gillett N. and Lasky L.A. (1993). Glycosylation-dependent cell adhesion molecule 1 (GlyCAM 1) mucin is expressed by lactating mammary gland epithelial cells and is present in milk. J. Clin. Investig. 92(2), 952-960.
Fonseca I., Antunes G.R., Paiva D.S., Lange C.C., Guimarães S.E.F. and Martins M.F. (2011). Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. Genet. Mol. Res. 10, 1295-1303.
Garman S.C., Sechi S., Kinet J.P. and Jardetzky T.S. (2001). The analysis of the human high affinity IgE receptor FcεRIα from multiple crystal forms. J. Mol. Biol. 311(5), 1049-1062.
Groenen M.A.M., Dijkhof R.J.M. and Ban der Poel J.J. (1995). Characterization of a glycosylation-dependent cell adhesion molecule 1 (GlyCAM1) like gene which is highly and specifically expressed in the lactating bovine mammary gland. Gene. 158(2), 189-195.
Hemati D.V., Rahimi M.G. and Farhadi A. (2014). Association between bovine lactoferrin gene varient and somatic cell count in milk based on EcoRΙ restriction site. Iranian J. Vet. Res. 15(1), 62-65.
Hou Z., Bailey J.P., Vomachka A.J., Matsuda M., Lockefeer J.A. and Horseman N.D. (2000). Glycosylation-dependent cell adhesion molecule 1 (GlyCAM 1) is induced by prolactin and suppressed by progesterone in mammary epithelium. Endocrinology. 141(11), 4278-4283.
Huang J.M., Wang Z.Y., Ju Z.H., Wang C.F., Li Q.L., Sun T. and Zhong J.F. (2011). Two splice variants of the bovine lactoferrin gene identified in Staphylococcus aureus isolated from mastitis in dairy cattle. Genet. Mol. Res. 10(4), 3199-3203.
Huiting S., Na N., Zhang X. and Zhao Y. (2017). The biological function and significance of CD74 in immune diseases. Inflamm. Res. 66(3), 209-216.
Kawai K., Korematsu K., Akiyama K., Okita M., Yoshimura Y. and Isobe N. (2015). Dynamics of lingual antimicrobial peptide, lactoferrin concentrations and lactoperoxidase activity in the milk of cows treated for clinical mastitis. Anim. Sci. J. 86(2), 153-158.
Le Provost F., Cassy S., Hayes H. and Martin P. (2003). Structure and expression of goat GLYCAM1 gene: Lactogenic-dependent expression in ruminant mammary gland and interspecies conservation of the proximal promoter. Gene. 313, 83-89.
Legrand D. and Mazurier J. (2010). A critical review of the roles of host lactoferrin in immunity. Biometals. 23(3), 365-376.
Livak K.J. and Schmittgen T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methodes. 25(4), 402-408.
Na N., Zhang X. and Zhao Y. (2017). The biological function and significance of CD74 in immune diseases. Inflamm. Res. 66(3), 209-216.
O'Halloran F., Bahar B., Buckley F., O'Sullivan O., Sweeney T. and Giblin L. (2009). Characterisation of single nucleotide polymorphisms identified in the bovine lactoferrin gene sequences across a range of dairy cow breeds. Biochimie. 91, 68-75.
Pawlik A., Sender G. and Korwin-Kossakowska A. (2009). Bovine lactoferrin gene polymorphism and expression in relation to mastitis resistance-a review. Anim. Sci. Pap. Rep. 27(4), 263-271.
Pawlik A., Sender G., Sobczyńska M., Korwin-Kossakowska A., Lassa H. And Oprządek J. (2014). Lactoferrin gene variants, their expression in the udder and mastitis susceptibility in dairy cattle. Anim. Prod. Sci. 55(8), 999-1004.
Peters M.D.P., Silveira I.D.B. and Fischer V. (2015). Impact of subclinical and clinical mastitis on sensitivity to pain of dairy cows. Anim. 9(12), 2024-2028.
SAS Institute. (2003). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Seegers H., Fouricho C. and Beaudeau F. (2003). Production effects related to mastitis and mastitis economics in dairy cattle herds. Vet. Rec. 34, 475-491.
Sharma N., Kang T.Y., Lee S., Kim J.N., Hur C.H., Ha J.C., Vohra V. and Jeong D.K. (2013). Status of bovine mastitis and associated risk factors in subtropical Jeju Island, South Korea. Trop. Anim. Health Prod. 45, 1829-1832.
Su H., Na N., Zhang X. and Zhao Y. (2017). The biological function and significance of CD74 in immune diseases. Inflamm. Res. 66, 209-216.
Swanson K.M., Stelwagen K., Dobson J., Henderson H.V., Davis S.R., Farr V.C. and Singh K. (2009). Transcriptome profiling of Streptococcus uberis-induced mastitis reveals fundamental differences between immune gene expression in the mammary gland and in a primary cell culture model. J. Dairy Sci. 92, 117-129.
Teng C.T. (2002). Lactoferrin gene expression and regulation: An overview. Biochem. Cell Biol. 80(1), 7-16.
Woof J.M. and Burton D.R. (2004). Human antibody–Fc receptor interactions illuminated by crystal structures. Nat. Rev. Immunol. 4, 89-99.
