Effects of Various Activators on Bovine Embryonic Development Following Intracytoplasmic Sperm Injection
الموضوعات :
K. Tasripoo
1
(
Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Street, Phathumwan, Bangkok 10330, Thailand
)
K. Srisakwattana
2
(
Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Street, Phathumwan, Bangkok 10330, Thailand
)
W. Nualchuen
3
(
Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Street, Phathumwan, Bangkok 10330, Thailand
)
S. p Sophon
4
(
Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Street, Phathumwan, Bangkok 10330, Thailand
)
الکلمات المفتاحية: activators, bovine embryonic development, intracytoplasmic sperm injection,
ملخص المقالة :
The objective of this study was to compare the effectiveness of various combination activators of bovine oocyte following intracytoplasmic sperm injection (ICSI) in terms of cleavage and embryo development. Bovine ICSI oocytes were treated with a variation of treatments of combined or single activators as follows; 30 V for 15 x 2 μsec of electrical pulse +10 μg/mL cycloheximide (CHX),7% ethanol + CHX, 5 μM Calcium ionophore A23187 (Ca-I)+ CHX, Ca-I + 1.9 mM 6-dimethylaminopurine (6-DMAP), CHX alone, Ca-I alone. The highest cleavage (73.5%-79.7%), and blastocyst rates (28.0%-35.3%) were obtained in Ca-I + 6-DMAP, ethanol + CHX and electrical stimuli + CHX groups. The treatment with CHX only or Ca-I only gave a significantly lower (P<0.05) yield of cleavage rate and blastocyst than those treatments with Ca-I + 6-DMAP, ethanol + CHX, electrical stimulationand Ca-I + CHX. In conclusion, these results show that post activation of ICSI oocytes with Ca-I + 6-DMAP, ethanol + CHX, electrical pulse + CHX and Ca-I + CHX are more effective treatments for increasing blastocyst development than those CHX only or Ca-I only, however, activation with ethanol + CHX is recommended.
Ajduk A., Yamauchi Y. and Ward M.A. (2006). Sperm chromatin remodeling after intracytoplasmic sperm injection differs from that of in vitro fertilization. Biol. Reprod. 75, 442-451.
Atabay E.C., Atabay F.P., de Vera R.V., Mamuad F.V. and Cruz L.C. (2006). Chemical and electrical activation of swamp buffalo (Bubalus bubalis) oocytes with or without cycloheximide treatments. Buffalo J. 21(2), 121-130.
Chung J.T., Keefer C.L. and DowneyB.R. (2000). Activation of bovine oocytes following intracytoplasmic sperm injection (ICSI). Theriogenology.53, 1273-1284.
Fatehi A.N., Bevers M.M., Schoevers E., Roelen B.A.J., Colenbrander B. and Gadella B.M. (2006). DNA damage in bovine sperm does not block fertilization and early embryonic development but induced apoptosis after the first cleavages. J. Androl. 27(2), 176-188.
Fujinami N., Hosoi Y., Kato H., Matumoto K., Saeki K. and Iritani A. (2004). Activation with ethanol improves embryo development of ICSI-derived oocytes by regulation of kinetics of MPF activity. J. Reprod. Dev.50(2), 171-178.
Galli C., VassilievI., Lagutina I., Galli A. and Lazzari G. (2003). Bovine embryo development following ICSI: effect of activation, sperm capacitation and pre-treatment with dithiothreitol. Theriogenology. 60, 1467-1480.
Garcia-Rosello E., Matas C., Canovas S., Moreira P.N. Gadea J. and Coy P. (2006). Influence of sperm pretreatment on the efficiency of intracytoplasmic sperm injection in pig. J. Androl. 27(2), 268-275.
Goto K., Kinoshita A. Takuma Y. and Ogawa K. (1990). Fertilization of bovine oocytes by the injection of immobilized, killed spermatozoa. Vet. Rec.127, 517-520.
Horiuchi T., Emuta C., Yamauchi Y., Oikawa T., Numabe. and Yanagimachi R. (2002). Birth of normal calves after intracytoplasmic sperm injection of bovine oocytes: a methological approach. Theriogenology. 57, 1013-1024.
Houdebine L.M. (2002). Transgenesis to improve animal production. Livest. Prod. Sci. 74(3), 255-268.
Keefer C.L., Younis A.I. and Blackett B.G. (1990). Cleavage development of bovine oocytes fertilized by sperm injection. Mol. Reprod. Dev. 25, 281-285.
Kubota C., Yamakuchi H., Todoroki J., Mizoshita K., Tabara N., Barber M. and Yang X. (2000). Six cloned calves produced from adult fibroblast cells after long-term culture. Proc. Natl. Acad. Sci. USA. 97, 990-995.
Lee J.W., Tien X.C. and Yang X.Z. (2003). Failure of male pronucleus formation is the major cause of lack of fertilization and embryo development in pig oocytes subjected to intracytoplasmic sperm injection. Biol. Reprod. 68, 1341-1347.
Liang Y.Y., Sangmalee A., Imsoonthornruksa S.,Lorthongpanich C., Srirattana K.,Sripunya N., Phewsoi W., Keawmungkun K., Laowtammathron C., Ye D., Ketudat-Cairns M. and Parnpai R. (2009). Effect of chemical activation treatments on the development of swamp buffalo (Bubalus bubalis) follicular oocytes following intracytoplasmic sperm injection. Pp. 78-86 in Proc.47th Kasetsart University Annual Conference: Veterinary Medicine, Bangkok (Thailand).
Liang Y.Y., Ye D.N., Laowtammathron C., Phermthai T., Nagai T., Somfai T. and Parnpai R. (2011). Effects of chemical activation treatment on the development of swamp buffalo (Bubalus bubalis) oocytes matured in vitro and fertilized by intracytoplasmic sperm injection. Reprod. Domest. Anim. 46(1), 67-73.
Lu Y.Q., Wang W.L., Yang H., Liang X.W., Liang Y.Y., Zhang M., Lu S.S., Kitiyanant Y. and Lu K.H. (2006). Flow-cytometric sorting the sperm and production of sex-preselected embryo in buffalo (Bubalus bubalis). Pp. 155-161 inProc. 5th Asian Buffalo Congress, Nanning, China.
Moisyadi S., Kaminski J.M. and Yanagimachi R. (2009). Use of intracytoplasmic sperm injection (ICSI) to generate transgenic animals. Com. Immuno, Microbiol. Infect. Dise. 32, 47-60.
Niemann H. and Kues W.A. (2000). Transgenic livestock: premises and promises. Anim. Prod. Sci. 60-61, 277-293.
Oikawa T., Takada N., Kikuchi T., Numabe T., Takenaka M. and Horiuchi T. (2005). Evaluation of activation treatments for blastocyst production and birth of viable calves following bovine intracytoplasmic sperm injection. Anim. Reprod. Sci. 86, 187-194.
RhoG.J., Kawarsky S., Johnson W.H., Kochhar K.. and Betterridge K.J. (1998). Sperm and oocyte treatments to improve the formation of male and female pronuclei and subsequent development following intracytoplasmic sperm injection into bovine oocytes. Biol.Reprod. 59, 918-924.
ShiraziA., Ostad-Hosseini S., Ahmadi E., Heidari B. and Shams-Esfandabadi N. (2009). In vitro developmental competence of ICSI-derived activated ovine embryos. Theriogenology.71, 342-348.
Suttner R., Zakhartchenko V., Stojkovic P., Muller S., Alberio R., Medjugorac I., Brem G., Wolf E. and Stojkovic M. (2000). Intracytoplasmic sperm injection in bovine : effects of oocyte activation,sperm pretreatment and injection technique. Theriogenology. 54, 935-948.
Tasripoo K., Srisakwattana K., Sophon S., Nualchuen W. and Usawang S. (2007). Cloning of buffalo fibroblast cell from donor of different ages. Buffalo J. 22(2), 141-152.
Tian J-H., Wu Z.H., Liu L., Cai Y., Zeng S.M., Zhu S.E., Liu G.S., Li Y. and Wu C.X. (2006). Effects of oocyte activation and sperm preparation on the development of porcine embryos derived from in vitro-matured oocytes and intracytoplasmic sperm injection.Theriogenology. 66, 439-448.
Wei H. and FukuiY. (2002). Births of calves derived from embryos produced by intracytoplasmic sperm injection without exogenous oocyte activation. Zygote. 10,149-153.
Yang X., Presicce G.A., Moraghan L., Jiang S.E. and Foote R.H. (1994). Synergistic effect of ethanol and cycloheximide on activation of freshly matured bovine oocytes. Theriogenology. 41, 395-403.
ZimmermannU.and Vienken J. (1982). Electric field-induced cell-to-cell fusion. J. Mem. Biol. 6, 165-182.
