Novel Single Nucleotide Polymorphisms (SNPs) in Two Oogenesis Specific Genes (BMP15, GDF9) and Their Association with Litter Size in Markhoz Goat (Iranian Angora)

الموضوعات :

ب. عارف‌نژاد ¹ (Department of Animal Science, Faculty of Agriculture, University of Shiraz, Shiraz, Iran)
ی. مهدی‌زاده ² (Department of Animal Science, Faculty of Agriculture, University of Shiraz, Shiraz, Iran)
آ. جوانمرد ³ (Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran)
م.ج. ضمیری ⁴ (Department of Animal Science, Faculty of Agriculture, University of Shiraz, Shiraz, Iran)
ع. نیازی ⁵ (Department of Biotechnology, Faculty of Agriculture, University of Shiraz, Shiraz, Iran)

تاريخ الإرسال : 15 الجمعة , ذو الحجة, 1437 تاريخ التأكيد : 17 الأحد , ربيع الثاني, 1438 تاريخ الإصدار : 13 الخميس , جمادى الثانية, 1439

الکلمات المفتاحية: polymorphism, fecundity, &lt, i&gt, BMP15&lt, /i&gt, , &lt, i&gt, GDF9&lt, /i&gt, ,

ملخص المقالة :

BMP15 and GDF9 are two oogenesis specific genes play a pivotal role in female fertility in mammals and potential for improvement of prolificacy in marker-assisted selection. The aim of present research was to investigate the variation and association between BMP15 and GDF9 polymorphism and litter size in Markhoz goats. The sequence variability of the different amplified fragments utilized for genotyping was further investigated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing. Sequencing technology allowed the detection of two nucleotide variations: A both non-functional mutation (G/A) transition at nt 1026 known as P3) and a synonymous mutation at 1049 position which for the first time was found at the BMP15 gene. Also, GDF9 gene exhibited a polymorphism on BcnI site (1159 bp) within exon 2 of amplified PCR products (glutamine/proline). Our analysis using general linear model (GLM) statistical methods showed litter size was significantly influenced by genotypes of GDF9 (P<0.01). Heterozygous genotype showed higher litter size than homozygous genotypes (P<0.01). The conformation of this founding would need large sample size and test to screen in other indigenous goats.

المصادر:

Abdel-Rahman S.M., Mustafa Y.A., AbdErrasool H.A., El Hanafy A.A. and Elmaghraby A.M. (2013). Polymorphism in BMP15 gene and its association with litter size in Anglo-Nubian goat. Biotech. Anim. Husb. 29(4), 675-683.

Ahlawat S., Sharma R. and Maitra A. (2013). Screening of indigenous goats for prolificacy associated DNA markers of sheep. Gene. 517(1), 128-131.

Arefnejad B. (2007). Molecular analysis of BMP15 (bone morphogenetic protein-15) gene in Markhoz goats. MS Thesis. Shiraz Univ., Shiraz, Iran.

Chu M.X., Wu Z.H., Feng T., Cao G.L., Fang L., Di L., Huang D.W., Li X.W. and Li N. (2011). Polymorphism of GDF9 gene and its association with litter size in goats. Vet. Res. Commun. 35, 329-336.

Deldar-Tajangookeh H., Zare Shahneh A., Zamiri M.J., Daliri M., Kohram H. and Nejati-Javaremi A. (2009). Study of BMP15 gene polymorphism in Iranian goats. African J. Biotechnol. 8(13), 2929-2932.

Dutta R., Das B., Laskar S., Kalita D.J., Borah P., Zaman G. and Saikia D.P. (2013). Polymorphism, sequencing and phylogenetic characterization of growth differentiation factor 9 (GDF9) gene in Assam Hill goat. African J. Biotechnol. 12(50), 6894-6900.

Farag I.M., Abd El-Aziz K.B., Ahmed E.S., Abdelsalam A.Z.E., Soliman K.A., Darwish A.M.M. and Abu Shady A. (2013). Polymorphism of BMPR1B, BMP15 and Beta casein genes in two of Egyptian domestic goats. J. Appl. Sci. Res. 9(4), 3199-3211.

Farshad A., Akhondzadeh S., Zamiri M. and Sadeghi G. (2008). The estrous cycle of the Markhoz goat in Iran. Asian-Australasian J. Anim. Sci. 21, 1411-1415.

Ganjali H., Mohammadi G., Gallehdari H. and Afzalzadeh M.R. (2012). Determination of FecXB and FecXG mutations in Kamouri goats of Khouzestan province. Mill. Genet. 12(2), 3529-3535.

Hanrahan J.P., Gregan S.M., Mulsant P., Mullen M., Davis G.H., Powell R. and Galloway S.M. (2004). Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovisaries). Biol. Reprod. 70, 900-909.

Javanmard A., Azadzadeh N. and Esmailizadeh A.K. (2011). Mutations in bone morphogenetic protein 15 and growth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds. Vet. Res. Commun. 35, 157-167.

Kasiriyan M.M., Hafezian S.H. and Hassani N. (2011). Genetic polymorphism BMP15 and GDF9 genes in Sangsari sheep of Iran. Int. J. Genet. Mol. Biol. 3, 31-41.

Knight P.G. and Glister C. (2003). Local roles of TGFβ-super family members in the control of ovarian follicular development. Anim. Reprod. Sci. 78, 165-183.

Mehdizadeh Gazooei Y., Niazi A. and Zamiri M.J. (2013). Single nucleotide polymorphism analysis of the bone morphogenetic protein receptor IB and growth and differentiation factor 9 genes in Rayini goats (Capra hircus). J. Livest. Sci. Technol. 1(2), 45-50.

Mohammadi G.H. and Alimahmoudi M. (2011). Determination of polymorphism of FecB gene in Najdi and native goats of Khouzestan province by PCR-RFLP. J. Vet. Med. Lab. 3, 13-20.

Rashidi A. (2000). Genetic evaluation of economic traits in Markhoz goats. Ph D. Thesis. Tarbiat Moddares Univ., Tehran, Iran.

SAS Institute. (1999). SAS^®/STAT Software, Release 8. SAS Institute, Inc., Cary, NC. USA.

Shamik P., Sachinandan D., Subhasis B., Ramakant K., Paras Y., Jaspreet Singh A., Saibal C., Subhransu P., Biswajit B., Tirtha K.D. and Surender Lal G. (2009). Polymorphism of fecundity genes (BMPR1B, BMP15 and GDF9) in the Indian prolific Black Bengal goat. Small Rumin. Res. 85(2), 122-129.

Silva B.D. (2005). Expression of growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), and BMP receptors in the ovaries of goats. Mol. Reprod. Dev. 70, 11-19.

Tavakolian J. (2000). An Introduction to Genetic Resources of Native Farm Animals in Iran. Animal Science Genetic Research Institute Press, Tehran, Iran.

Wang Y., Yuanxiao L., Nana Z., Zhanbin W. and Junyan B. (2011). Polymorphism of Exon2 of BMP15 gene and its relationship with litter size of two Chinese goats. Asian-Austral-asian J. Anim. Sci. 24(7), 905-911.

Wang Y., Zhang X. and Chu M. (2013). Polymorphisms of caprine GDF9 gene and their association with litter size in Henan dairy goat. J. Anim. Vet. Adv. 12(21), 1590-1596.

Xue-qin R.A.N., Jian-bin L.I.N., Zhi-yong D.U., Cheng Q.I.N.G. and Jia-fu W.A.N.G. (2009). Diversity of Bmp15 and Gdf9 genes in White goat of Guizhou province and evolution of the encoded proteins. Zool. Res. 30(6), 593-602.

Yeh F.C., Yang R.C., Boyle T.B.J., Ye Z.H. and Mao J.X. (1997). PopGene, the User-Friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.

Yuanqing H., Xiaoke M., Xiaoyong L., Cunxia Z. and Jun L. (2009). Candidate genes polymorphism and its association to prolificacy in Chinese goats. J. Agric. Sci. 2(1), 88-92.

Zhang H.Y., Ding X.L., Ying S.J., Wang Z.Y., Pang X.S., Wang R.F., Chen Q.K., Shi J.F., Zhang H. and Wang F. (2008). SSCP analysis on exon 2 of GDF9 gene in local goat breeds of Jiangsu province. Jiangsu Agric. Sci. 5, 51-53.

Zhu G.Q., Wang Q.I., Kang Y.G., Lv Y.Z. and Cao B.Y. (2013). Polymorphisms in GDF9 gene and its relationship with litter size in five breeds of Black goats. Iranian J. Appl. Anim. Sci. 3(3), 625-628.