Polymorphism of the Melatonin Receptor 1A Gene and Its Association with Litter Size in Zel and Naeini Sheep Breeds

الموضوعات :

ن. مرادی ¹ (Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ق. رحیمی ² (Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ن. نظیفی ³ (Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ع. نوربخش ⁴ (Department of Animal Science, Orumie University, Orumie, Iran)

تاريخ الإرسال : 23 السبت , صفر, 1434 تاريخ التأكيد : 06 الثلاثاء , جمادى الثانية, 1434 تاريخ الإصدار : 29 السبت , ربيع الثاني, 1435

الکلمات المفتاحية:

ملخص المقالة :

The influence of melatonin receptor 1A gene on litter size was studied in 150 ewes from Zel (n=100) and Naeini (n=50) sheep breeds. Two restriction fragment length polymorphism (RFLP) analyses were done to determine all genotypes occurring by variations at MnlI and RsaI restriction sites. The M and C were the most frequent alleles in both of these breeds. The MM genotype was predominant in Zel and Naeini breeds (0.52 and 0.60, respectively). The CT genotype (0.45) has the highest frequency in Zel and the CC genotype (0.44) was predominant in Naeini breed. The MMCT genotype was identified with the highest frequency of 0.25. There were no significant differences between melatonin receptor 1A (MTNR 1A) genotypes and litter size in Naeini ewes. But it seems that selection of animals with mm and Mm genotypes can progress mean litter size in Zel breed. The positive effect of mC and mT alleles was significant on lambing rate in Zel and Naeini breeds. However, the mmCT genotype has considerably greater mean litter size than the other ones. Finally, the MTNR 1A polymorphism can explain only a small part of the genetic variability on seasonal sexual activity between these breeds. Therefore, the implication of other genes must be noticed.

المصادر:

Al Shorepy S.A. and Notter D.R. (1996). Genetic variation and covariation for ewe reproduction, lamb growth and lamb scrotal circumference in a fall-lambing sheep flock. J. Anim. Sci. 74, 1490-1498.
Al Shorepy S.A. and Notter D.R. (1997). Response to selection for fertility in a fall-lambing sheep flock. J. Anim. Sci. 75, 2033-2040.
Barrett P., Conway S., Jockers R., Strosberg A.D., Guardiola-Lemaitre B., Delagrange P. and Morgan P.J. (1997). Cloning and functional analysis of a polymorphic variant of the ovine Mel 1A melatonin receptor. Biochim. Biophys. Acta. 27, 299-307.
Bittman E.L., Dempsey R.J. and Karsch F.J. (1983). Pineal melatonin secretion drives the reproductive response to daylength in the ewe. Endocrinology. 113, 2276-2283.
Carcangiu V., Luridiana S., Vacca G.M., Daga C. and Mura M.C. (2011). A polymorphism at the melatonin receptor 1A (MTNR 1A) gene in Sarda ewes affects fertility after AI in the spring. Reprod. Fertil. Dev. 23, 376-380.
Carcangiu V., Vacca G.M., Mura M.C., Dettori M.L., Pazzola M., Luridiana S. and Bini P.P. (2009). Relationship between MTNR 1A melatonin receptor gene polymorphism and seasonal reproduction in different goat breeds. Anim. Reprod. Sci. 110, 71-78.
Carcangiu V., Vacca G.M., Parmeggiani A., Mura M.C. and Bini P.P. (2005). Blood melatonin levels relating to the reproductive activity of Sarda does. Small Rumin. Res. 59, 7-13.
Chabot V., Caldani M., De Reviers M.M. and Pelletier J. (1998). Localization and quantification of melatonin receptors in the diencephalon and posterior telencephalon of the sheep brain. J. Pineal. Res. 24, 50-57.
Chu M.X., Cheng D.X., Liu W.Z., Fang L. and Ye S.C. (2006). Association between melatonin receptor 1A gene and expression of reproductive seasonality in sheep. Asian-australas J. Anim. Sci. 19, 1079-1084.
Chu M.X., Cl J.I. and Chen G.H. (2003). Association between PCR-RFLP of melatonin receptor l a gene and high prolificacy in Small Tail Han sheep. Asian-australas J. Anim. Sci. 16, 1701-1747.
Davis G.H. (2005). Major genes affecting ovulation rate in sheep. Genet. Sel. Evol. 37, 11-23.
Drew J.E., Barrett P., Williams L.M., Conway S. and Morgan P.J. (1998). The ovine melatonin-related receptor: cloning and preliminary distribution and binding studies. J. Neuroendocrinol. 10, 651-661.
Dubocovich M.L. (1995). Melatonin receptors: are there multiple subtypes? Trends Pharmacol. Sci. 16, 50-56.
Dubocovich M.L., Yun K., Al Ghoul W.M., Benloucif S. and Masana M.I. (1998). Selective MT2 melatonin receptor antagonists block melatonin mediated phase advances of circadian rhythms. Faseb. J. 12, 1211-1220.
Ebisawa T., Karne S., Lerner M.R. and Reppert S.M. (1994). Expression cloning of a high affinity melatonin receptor from Xenopus dermal melanophores. Proc. Natl. Acad. Sci. USA. 91, 6133-6137.
Goldman B.D. (2001). Mammalian photoperiodic system: formal properties and neuroendocrine mechanisms of photoperiodic time measurement. J. Biol. Rhythms. 16, 283-301.
Hazlerigg D. and Loudon A. (2008). New insights into ancient seasonal life timers. Curr. Biol. 18, 795-804.
Hernández X., Bodin L., Chesneau D., Guillaume D., Allain D., Chemineau P., Malpaux B. and Migaud M. (2005). Relationship between MT1 melatonin receptor gene polymorphism and seasonal physiological responses in Ile-de-France ewes. Reprod. Nutr. Dev. 45, 151-162.
Kaczor U., Kmiecik M., Molik E. and Rychlik T.(2006). Polymorphism in the melatonin receptor gene MT1 (locus MTNR 1A) in sheep. Arch. Tierz. 49, 257-262.
Karsch F.J., Bittman E.L., Foster D.L., Goodman R.L., Legan S.J. and Robinson J.E. (1984). Neuroendocrine basis of seasonal reproduction. Recent. Prog. Horm. Res. 40, 185-232.
Khaldari M. (2004). Sheep and Goat husbandry. Jahad daneshgahi pushers, Tehran, Iran.
Lincoln G.A. and Clarke I.J. (1994). Photoperiodically-lnduced cycles in the secretion of prolactin in hypothalamo-pituitary disconnected rams: evidence for translation of the melatonin signal in the pituitary. Gland. J. Neuroendocrinol. 6, 251-260.
Malpaux B., Thiery J.C. and Chemineau P. (1999). Melatonin and the seasonal control of reproduction. Reprod. Nutr. Dev. 39, 355-366.
Malpaux B., Viguié C., Skinner D.C., Thiery J.C., Pelletier J. and Chemineau P. (1996). Seasonal breeding in sheep: mechanism of action of melatonin. Anim. Reprod. Sci. 42, 109-117.
Mateescu R.G., Lunsford A.K. and Thonney M.L. (2009). Association between melatonin receptor 1A gene polymorphism and reproductive performance in Dorset ewes. J. Anim. Sci. 87, 2485-2488.
Messer L.A., Wang L., Tuggle C.K., Yerle M., Chardon P., Pomp D., Womack J.E., Barendse W., Crawford A.M., Notter D.R. and Rothschild M.F. (1997). Mapping of the melatonin receptor 1A (MTNR 1A) gene in pigs, sheep and cattle. Mamm. Genome. 8, 368-370.
Migaud M., Gavet S. and Pelletier J. (2002). Partial cloning and polymorphism of the melatonin 1A (Mel 1A) receptor gene in two breeds of goat with different reproductive seasonality. Reproduction. 124(1), 59-64.
Miller S.A., Dykes D.D. and Polesky H.F. (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids. Res. 16, 1215.
Mura M.C., Luridiana S., Vacca G.M., Bini P.P. and Carcangiu V.(2010). Effect of genotype at theMTNR 1Alocus and melatonin treatment on first conception in Sarda ewe lambs. Theriogenology. 74, 1579-1586.
Notter D.R. (2008). Genetic aspects of reproduction in sheep. Reprod. Domest. Anim. 43, 122-128.
Notter D.R. and Cockett N.E. (2005). Opportunities for detection and use of QTL influencing seasonal reproduction in sheep: a review. Genet. Sel. Evol. 37, 39-53.
Notter D.R., Cockett N.E. and Hadfield T.S. (2003). Evaluation of melatonin receptor 1A as a candidate gene influencing reproduction in an autumn lambing sheep flock. J. Anim. Sci. 81, 912-917.
Pandi Perumal S.R., Srinivasan V., Maestroni G.J.M., Cardinali D.P., Poeggeler B. and Hardeland R. (2006). Melatonin. Febs. J.  273, 2813-2838.
Pelletier J., Bodin L., Hanocq E., Malpaux B., Teyssier J., Thimonier J. and Chemineau P. (2000). Association between expression of reproductive seasonality and alleles of the gene for Mel 1A receptor in the ewe. Biol. Reprod. 62, 1096-1101.
Reppert S.M., Weaver D.R., Cassone V.M., Godson C. and Kolakowski L.F. (1995). Melatonin receptors are for the birds: molecular analysis of two receptor subtypes differentially expressed in chick brain. Neuron. 15, 1003-1115.
Reppert S.M., Weaver D.R. and Ebisawa T. (1994). Cloning and characterization of a mammalian melatonin receptor that mediates reproductive and circadian responses. Neuron. 13, 1177-1185.
Reppert S.M., Weaver D.R. and Godson C. (1996). Melatonin receptors step into the light: cloning and classification of subtypes. Trends. Pharmacol. Sci. 17, 100-102.
Seker I., Özmen O., Çinarkul B. and Ertugrul O.(2011). Polymorphism in melatonin receptor 1A (MTNR 1A) gene in Chios, White Karaman and Awassi sheep breeds. Kafkas Univ. Vet. Fak. Derg. 17, 865-868.
Thiery J.C., Chemineau P., Hernandez X., Migaud M. and Malpaux B. (2002). Neuroendocrine interactions and seasonality. Domest. Anim. Endocrine. 23, 87-100.
Trecherel E., Batailler M., Chesneau D., Delagrange P., Malpaux B., Chemineau P. and Migaud M. (2010). Functional characterization of polymorphic variants for ovine MT1 melatonin receptors: possible implication for seasonal reproduction in sheep. Anim. Reprod. Sci. 122, 328-334.
Von Gall C., Stehle J.H. and Weaver D.R. (2002). Mammalian melatonin receptors: molecular biology and signal transduction. Cell. Tissue. Res. 309, 151-162.
Von Gall C., Weaver D.R., Moek J., Jilg A., Stehle J.H and Korf H.W. (2005). Melatonin plays a crucial role in the regulation of rhythmic clock gene expression in the mouse pars tuberalis. Ann. New York. Acad. Sci. 122, 508-511.
Weaver D.R., Liu C. and Reppert S.M. (1996). Nature's knockout: the Mel1b receptor is not necessary for reproductive and circadian responses to melatonin in Siberian hamsters. Mol. Endocrinol. 10, 1478-1487.
Wright C.W. (2000). Polymorphisms at the melatonin (MTNR 1A) gene and their association to reproductive performance in fall lambing ewes. MS Thesis. South Dakota State Univ., Brookings.