Polymorphism and Sequencing of DGAT1 Gene in Iranian Holstein Bulls

الموضوعات :

M. Hosseinpour Mashhadi ¹ (Department of Animal Science,Mashhad Branch, Islamic Azad University, Mashhad, Iran)
M.R. Nassiri ² (Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran)
M. Mahmoudi ³ (Immunology Research Center, Bu Ali Research Institute, Mashhad Universityof Medical Science, Mashhad, Iran)
M. Rastin ⁴ (Immunology Research Center, Bu Ali Research Institute, Mashhad Universityof Medical Science, Mashhad, Iran)
N.E.J. Kashan ⁵ (Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran)
R. Vaez Torshizi ⁶ (Department of Animal Science, Tarbiat Modares University, Tehran, Iran)
N. Tabasi ⁷ (Immunology Research Center, Bu Ali Research Institute, Mashhad Universityof Medical Science, Mashhad, Iran)
S.E. Nooraee ⁸ (Department of Animal Science,Mashhad Branch, Islamic Azad University, Mashhad, Iran)

تاريخ الإرسال : 15 الجمعة , ربيع الأول, 1432 تاريخ التأكيد : 18 الجمعة , جمادى الأولى, 1432 تاريخ الإصدار : 08 الخميس , ربيع الثاني, 1433

الکلمات المفتاحية: PCR-RFLP, Holstein, gene, DGAT1,

ملخص المقالة :

Quantitative traits locus for milk production traits has been described on centromeric end of bovine chromosome 14. Reports name the acyl coA: diacylglycerol acyltransferase (DGAT1) gene as a potential candidate gene with dinucleotide substitution (AA to GC) in exon VIII which causes the change of lysine to alanine in amino acid (K232A).The aim of the present study was to estimate the frequency of DGAT1 K232A polymorphism in Iranian Holstein bulls as a potential quantitative trait locus (QTL) for marker assisted selection. Sample of 103 Holstein bulls from the Animal Breeding Center of Iran were genotyped for DGAT1 polymorphism (A and K allele). The PCR-RFLP technique was used to study the DGAT1 gene polymorphism. Frequency of KK, KA and AA genotypes were 0.59, 0.41 and zero respectively. The allele frequencies of the DGAT1 gene were 0.7961 and 0.2039 for K and A allele, respectively. The K allele was sequenced and registered in NCBI gene bank with EU075528 accession number.

المصادر:

Boichard D.C., Grohs F., Bourgeois F., Cerqueira R., Faugeras A., Neau R., Rupp Y., Amigues M., Boscher Y. and Levézil H. (2003). Detection of genes influencing economic traits in three French dairy cattle breeds. Genet Sel Evol. 35, 77-101.
Chandra S., Czarnik P.U., Zabolewicz T., Pareek R.S. and Walawski K. (2005). DGAT1 K232A quantative trait nucleotide polymorphism in Polish Black-and-White cattle. J. Appl. Genet. 46, 85-87.
Citek J., Rehout Hradecka E., Vecerek L. and Panicke L. (2004). The breeding values of german holstien sires and the DGAT1 polymorphism. Anim. Sci. Papers and Reports. 22(2), 2. 19-23.
Coppieters W., Riquet J., Arranz J.J., Berzi P., Cambisano N., Grisart B., Karim L., Marcq F., Moreau L., Nezer C., Simon P., Vanmanshoven P., Wagenaar D. and Georges M. (1998). A QTL with major effect on milk yield and composition maps to bovinechromosome 14. Mamm. Genome. 9, 540-544.
Farniar F., Grisart B., Coppieters W., Riquet J., Berzi P., Cambisano N., Karim L., Mni S., Moisio M., Simon P., Wagenaar D., Vilkki J. and Georges M. (2002). Simultaneous mining of linkage and linkage disequilibrium to fine map quantitative trait loci in out bred half-sib pedigrees: Revisiting the location of a quantitative trait locus with major effect on milk production on bovine chromosome 14. Genetics. 161, 275-287.
Grisart B., Coppieters W., Farnir F., Karim L., Ford C., Berzi P., Cambisano N., Mni N., Reid S., Simon P., Spelman R., Georges M. and Snell R. (2001). Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine and composition. Genome Research. 12, 222-231.
Grisart B., Farnir F., Karim L., Cambisano N., Kim J.J., Kvasz A., Mni N., Simon P., Frere J.M., Coppieters W. and Georges M. (2004). Genetic and functional confirmation of the causality of the DGAT1 K232A quantative trait nucleotide in affecting milk yield and composition. PNAS. 101(8), 2398-2403.
Heyen D.W., Weller J.I., Ron M., Band M., Beever J.E., Feldmesser E., Da Y., Wiggans G.R., VanRaden P.M. and Lewin H.A. (1999). A genome scan for QTL influencing milk production and health traits in dairy cattle. Physiol Genomics. 1, 165-175.
Hori-Oshima S. and Barreras- Serran A. (2003). Relationships between DGAT1 and PIT1 genes polymorphism and milk yield in Holstein cattle. J. Anim. Sci. 8(1), 252.
Kaupe B., Winter A., Fries R. and Erhardt G. (2004). DGAT1 polymorphism in Bos indicus and Bos Taurus. J. Dairy Res. 71, 182-187.
Kaupe B., Brandt H., Prinzenberg E.M. and Erhardt G. (2007). Joint analysis of the influence of CYP11B1 and DGAT1 genetic variation on milk production, somatic cell score, conformation, reproduction, and productive lifespan in German H-Holstein cattle. J. Anim. Sci. 85, 11-21.
Lacorte G.A., Machado M.A., Martinez M.L., Campos A.L., Maciel R.P., Verneque P.S., Teodoro R.L., Peixoto M.G.C.D., Carvalho M.R.R. and Fonseca C.G. (2006). DGAT1 K232A polymorphism in Brazilian cattle breeds. Gent. Mol. Res. 5, 475-482.
Looft C., Reinsch N., Karall-Albrecht C., Paul S., Brink M., Thomsen H., Brockmann G., Kuhn C., SchwerinM. and Kalm E. (2001). A mammary gland EST showing linkage disequilibrium to a milk production QTL on bovine Chromosome 14. Mamm. Genome. 12, 646-650.
Ripoli M.V., Corva P. and Giovambattista G. (2006). Analysis of a polymorphism in the DGAT1 gene in 14 cattle breeds through PCR-SSCP methods. Res. Vet. Sci. 80, 287-290.
Riquet J., Coppieters, W., Cambisano N., Arranz J.J., Berzi P., Davis S.K., Grisart B., Farnir F., Karim L., Mni M., Simon P., Taylor J.F., Vanmanshoven P., Wagenaar D., Womack J.E. and Georges M. (1999). Fine-mapping of quantitative trait loci by identity by descent in out bred populations: application to milk production in dairy cattle. Pp 9252-9257 in Proc. Natl. Acad. Sci. USA.
Sanger F., Nicklen S. and Coulson A.R. (1977). DNA sequencing with chain terminating inhibitors. Pp.  5463-5467 in Proc. Natl. Acad. Sci. USA.
Spelman R.G., Ford C.A., McElhinney P., Gregory G.C. and Snell R.G. (2002). Characterization of the DGAT1 gene in the New Zealand dairy population. J. Dairy Sci. 85, 3514-3517.
Thaller G., KrämerW., Winter A., Kaupe B., Erhardt G. and Fries R. (2003). Effect of DGAT1 variants on milk production traits in German cattle breeds. J. Anim. Sci. 81, 1911-1918.
Winter A., Alzinder A. and Fries R. (2002). Physical mapping of a bovine chromosome region with an effect on milk fat content. PhD Thesis. Technische Universität München. Germany.
Winter A., Alzinger A. and Fries R. (2004). Assessment of the gene content of the chromosomal regions flanking bovine DGAT1. Genomics. 83, 172-180.
Yeh F.C. and Yong R.C. (1999). PopGene Version 1.31. University of Alberta.