Caffeine effect on adenosine deaminase catalysis: A new look at the effect of caffeine on adenosine deaminase activity
الموضوعات : Iranian Journal of CatalysisRoya Mahinpour 1 , Majid Ghasemi 2 , Zahra Moosavi-Nejad 3 , Zohreh Zahraie 4
1 - Department of Biotechnology, Faculty of Chemistry, University of Kashan, Kashan, Iran.
2 - Department of Organic Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran.
3 - Departmentof Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
4 - Department of Biotechnology, Faculty of Chemistry, University of Kashan, Kashan, Iran.
الکلمات المفتاحية: Caffeine, Inhibition, Adenosine deaminase, Homotropic effect,
ملخص المقالة :
The effect of physiological concentrations of caffeine (purified from Persian tea) on adenosine deaminase (ADA) activity at physiological and pathological concentrations of adenosine (as substrate) in 50 mM Tris-HCl buffer (pH 7.3) at 37°C was investigated, using UV-VIS spectroscopy. ADA exhibited a bi-phasic activity behavior and both phases showed positive cooperativities indicating adenosine has at least two regulatory sites on ADA molecule. We proposed a mechanism for the inhibitory effect of caffeine on ADA catalytic activity by analyzing ADA kinetic behavior at various physiological concentrations of caffeine (10, 30, 70 mM): Caffeine dominantly resulted in decreased catalytic activity of ADA via reducing the positive homotropic effect of adenosine on ADA activity. Surprisingly, it also showed a very weak but significant activatory effect on ADA activity in a concentration-dependent manner. To the best of our knowledge, this is the first report on the dual effect of caffeine on ADA activity.
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