Evaluation of antibacterialic activity of methanol extracts of Francoeuria Undulata in two harvest times on several aerobic bacteria causing vaginal infections
الموضوعات : مجله گیاهان داروییلیلا فیروزیان 1 , لیلا امجد 2 , منیر دودی 3
1 - واحد فلاورجان، دانشگاه ازاد اسلامی، اصفهان، ایران
2 - گروه بیولوژی، واحد فلاورجان، دانشگاه ازاد اسلامی، اصفهان، ایران
3 - گروه میکروبیولوژی، واحد فلاورجان، دانشگاه ازاد اسلامی، اصفهان، ایران
الکلمات المفتاحية: Antibacterial activity, methanol extract, Francoeuria Undulata,
ملخص المقالة :
Background & Aim:Vaginal disease has always been a great concern of women. Medicinal plants as natural resources with fewer side effects can effectively treat these infections given the diversity of these infections, resistance to antibiotics and side effects of existing drugs. The present study aimed to compare antimicrobial effects of methanol extract of Francoeuria Undulata on growth of several gram-positive and gram-negative bacteria causing vaginal infections.Experimental: Francoeuria Undulata as an indigenous Iranian plant was collected in two harvest times (T1 and T2). Methanol extract of aerial parts of the plant was prepared. The effects of various concentrations (62.5, 125, 250 and 500 mg/ml)of methanol extracts were studied onKlebsiella and Enterobacter as gram-negative pathogens. Minimum Inhibitory Concentration (MIC) of bacterial growth and MinimumBactericidal Concentration (MBC) were determined using macro-dilution method. Data analysis was performed using ANOVA.Results: The results showed that methanol extracts of aerial parts of Francoeuria Undulata inhibit bacterial growth of Klebsiella pneumoniae and Enterobacter aerogenes. MICs varied from 125mg/ml to 250 mg/ml in the old and new harvest times. Therefore, the smallest diameter of inhibition zone belonged to Klebsiella pneumoniae at 62.5mg/ml concentration in the old harvest time and the largestdiameter of inhibitory zone belonged tothe second strains of Klebsiellapneumoniae at 500mg/ml concentration in the new harvest time.Recommended applications/industries: The findings of the study indicated that methanol extracts of Francoeuria Undulata at two old and new harvest times had strong antibacterial and inhibitory effects on gram-negative bacteria causing vaginal infections.
Algabr, M.N. 2012. Essential oil composition of Pulicaria jaubertii from Yemen. International Journal of Medicinal and Aromatic Plants, 2(4): 688-690.
Amin, G. 2002. Screening of Iranian plants for antifungal activity. Daru, 9(1): 38-48.
Amjad, L., Mohammadi Kamalabadi, M. and Mohammadi Sichani, M. 2010. Antibacterial activity of methanol extracts of flowers and leaves of Achillea wilhelmsii. Journal of Qom University of Medical Sciences, 5(3): 50-56.
Barry, A., Garcia, J. and Thrupp, LD. 1970. Interpretation of sensitivity test results. American Journal of Clinical Pathology, 53:140-144.
Cowan, M. 1999. Plant products as antimicrobial agent. Clinic Microbiology Reviews, 12(14): 564–582.
Gerging, D.N., Larson, T.A. and Hughes, R.A. 1991. Antimicrobial agents. Chemotherapy, 35: 1284-1286.
Juven, B.J., kanner, J., Schved, F. and Weisslowicz, H. 2009. Factors that interact with the antibacterial action of Thyme essential oil and its active constituents. Iranian Journal of Dentist, 21(3):215-220.
Khanafari, A. and Hosseini, F. 2000. Scientific microbiology, Poursina Publication, Vol. I, Second Edition: 140-144.
Mardane, G. 2012. Medical microbiology: detection and clinical follow-up of diseases. Artistic – Davidson, 2011: 102-162.
McKeegan, K.S., Borges-Walmsley, M.I. and Walmsley, A.R. 2002. Microbial and viral drug resistance mechanisms. Trend Microbiology, 10(110): 8-14.
Mohammadi Sichani, M., Amjad, L. and Mohammadi Kamalabadi, M. 2010. Effects of methanol extract and essential oil of flowers of Achillea wilhelmsii on pathogenic bacteria. Journal of Medical Science, 13(3): 9-13.
Naghsh, A. 2013. Evaluation of antibacterial activity of essential oils and extracts of flowers of Artemisia deserti on several pathogenic bacteria, Master Thesis, Islamic Azad University, School of Biological Sciences, Flavarjan, pp. 132.
Rajabpour, M., Arabestani, M., Yousefi Mashouf, R. and Alikhani, M. E. 2013. Determination of MIC at three different antibiotic classes of clinical isolates of Pseudomonas aeruginosa from hospitalized patients in Hamedan hospital (2011-2012). Journal of Medical Microbiology, 7(3): 18-25.
Ravandeh, M., Valizadeh, J., Noroozifar, M. and Khorasani-Motlagh, M. 2011. Screening of chemical composition of essential oil, mineral elements and antioxidant activity in Pulicaria undulata L. Mey from Iran. Journal of Medicinal Plants Research, 5(10): 2035-2040.
Rezaei, M. B., Kamkar, G. and Mozaffarian, V. 2008. Studying chemical composition of extract of Anthemis coelopoda Boiss. Research Scientific Journal of Iran Medicinal and Aromatic Plants, 24: 274-277.
Shahnazi, S. 2007. Evaluation of chemical and antimicrobial properties of the extracts of Saturejaintermedia CA. Mey.Journal of Medicinal Plants, 1: 85-92.
Skocibusic, M., Bazic, N., Dunnkic, V. and Radonic, A. 2004. Antibacterial activity of Achillea clavenne essential oil against respiratory tract patogens. Fitoteropia. 75(7-8): 733-736.
Sokmen, A., Vardar-Unlu, G. and Polissiou, M. 2003. Antimicrobial activity of the essential oil and ethanol extract of Achillea sintenisii Hub.Mor.(Asteraceae). Phytotherapy Research, 17(9): 1005-1010.
Steven Pray, W. 2010. Treating vaginal fungal infection. Pharmacology, 35(9): 14-23.
Talebi, F. 2014. Evaluating antibacterial activity of essential oils and extracts of flowers of Achillea tenuifolia on several pathogenic bacteria. Master Thesis, Islamic Azad University, School of Biological Sciences, Flavarjan, pp. 183.
