Effect of Hydro-alcoholic Extract of Punica Granatum L. Peel on Ethylene Glycol-Induced Kidney Calculi in Wistar Rats
الموضوعات : مجله گیاهان دارویی
1 - گروه فیزیولوژی جانوری، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
2 - گروه فیزیولوژی جانوری، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
الکلمات المفتاحية: Nephrolithiasis, Punica granatum, Calcium oxalate, Ethylen Glycol,
ملخص المقالة :
Background & Aim: This study evaluated prevention effect of hydro-alcoholic extract of punica granatum Peel ethylene glycol-induced Nephrolithiasis in wistar rats. Experimental: 20 male Wistar rats were randomly divided into 4 equal groups. Group I animals served as normal control (A) and received distilled water for 28 days. Group II to IV animals received 1% v/v ethylene glycol (EG) in distilled water for 28 days. Group II served as EG or negative control (B). Group III and IV (preventive groups) received hydro-alcoholic extract of punica granatum Peel orally for 28 days in dose of 200 mg/kg (C) and 400 mg/kg (D) respectively. 24-hour urine samples were collected on day 0 and 28. Urine volume and urine parameters levels (oxalate, calcium and citrate) were measured. On day 28, blood was collected for biochemical parameters (craetinine, urea and uric acid). Animals were sacrificed and kidneys were harvested, histopathologically evaluated for calcium oxalate (CaOx) crystals. Results & Discussion: EG significantly increased urine oxalate and calcium, serum creatinine, urea and uric acid levels. It also significantly decreased urine citrate level. In C and D group resulted in lower levels of urine oxalate, calcium and serum creatinine, urea and uric acid as compared to Group II. Also resulted in significantly higher levels citrate as compared to Group II (P<0.05). Industrial and practical recommendations: hydro-alcoholic extract of punica granatum peel is effective in prevention of EG- induced nephrolithiasis in Wistar rats.
Bagri, P., Ali, M., Aeri, V., Bhowmik, M., Sultana, S. 2009. Antidiabetic effect of Punica granatum flowers: effect on hyperlipidemia, pancreatic cell lipid peroxidation and antioxidant enzymes in experimental diabetes. Food and Chemical Toxicology., 47: 50–54.
Biswas, D. 2012. Antilithiatic and nephroprotective activity of Punica granatum linn. on experimental animals. International Journal of Pharmacy & Life Sciences Life., 7: 976-712.
Fredric L., Coe., Andrew Evan., Elaine Worcester. 2005. Kidney stone disease. J. Clin. Invest., 115: 2598-2608.
Goljan, EF. 2007. Pathology. Philadelphia: Mosby Elsevier. 12: 23-32
Guyton, A., Hall, JE. 2011. Medical physiology. Translate Sepehri, H. Ghasemi, K. Publisher andisherafi. 31:504-509.
Henry, J. B. 1996. Clinical Diagnosis and Management by Laboratory Methods. Saunders Company, Toronto. 449-450.
Hadjzadeh, M., Khajavi Rad, A., Rajaei, Z. 2012. The preventive effect of N-butanol fraction of Nigella sativa on ethylene glycol-induced kidney calculi in rats. Pharmacogn Mag., 7(28): 338–343.
Halabe A., Shor R., Wong N. L., Sutton R. A. 2003. Effect of vitamin D3 on the conversion of ethylene glycol to glycolate and oxalate in ethylene glycol-fed rats. Clinical Chimie Acta., 330: 135-139.
Lansky, EP., Newman, RA. 2007. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. J. Ethnopharmacol., 109: 177-206.
Lee, C-Chen, L-G., Liang, W-L., Wang, C-C. 2010. Anti-inflammatory effects of Punicagranatum Linne in vitro and in vivo. J.Food Chemistry. 118:315-322.
Liebman, M., and Costa, G. 2000. Effects of calcium and magnesium on urinary oxalate excretion after oxalate loads. The Journal of Urology. 163-1565.
Li, Y. 2006. Evalution of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem., 96: 254-60.
Manjula, K., Rajendran, K., Eevera, T., Kumaran, S. 2012. Effect of Costus igneus stem extract on calcium oxalate urolithiasis in albino rats. Urol Res. 40(5):499-510.
Robbins, SL., Cotran RS. 2005. Cotranpathologic basis of disease. Philadelphia: Elsevier Saunders. 32:77-140.
Saeidi, J., Bozorgi, H., Zendehdel, A., Mehrzad, J. 2012. Therapeutic effects of aqueous extracts of Petroselinum Sativum on ethylene glycol- induced kidney calculi in rats. Urol Journal. 9(1): 61-66.
Turk, G., Sonmez, M., Avdin, M., Yuce, A., Gur, S., Yuksel, M., Aksu, E.H., Aksoy,H., 2008. Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone levels in male rats. Clinical Nutrition., 27: 287–296.
Vidhan, J., Ara, D.M., John, R.P. 2010. Anthocyanins and polyphenol oxidase from dried aerials of pomegranate (Punica granataum L.). Food Chemistry. 118: 11–16.
Viladomiu, M., Hontecillas, M., Lu, P., Bassaganya-Riera, J. 2013. Preventive and prophylactic mechanisms of action of pomegranate bioactive constituents. Evid Based Complement Alternat Med. doi: 10.1155/789764. [In press].
Zamani, Z. 1990. Characteristics of Pomegranate Cultivars Grown in Saveh oIran. M.Sc. Thesis. University of Tehran. pp: 175.
