Ameliorative properties of methanol leaf extract of Momordica charantia following alloxan-induced cardiotoxicity in rats
الموضوعات : مجله گیاهان دارویی
ساندی افوب
1
,
آدمولا اویابمی
2
,
تمیدایو اومواله
3
,
ادورابنرو آدداپو
4
,
آبیدون آیودل
5
,
مومح یاکوبو
6
,
الوفمی اگانتیبجو
7
,
آدلو آدداپو
8
1 - گروه فارماکولوژی و سم شناسی دانشکده دامپزشکی، دانشگاه ابادان ، نیجریه
2 - گروه فیزیولوژی و بیوشیمی دانشکده دامپزشکی، دانشگاه ابادان ، نیجریه
3 - گروه دامپزشکی دانشگاه ابادان ابادان ، نیجریه
4 - گروه فارماکولوژی، دانشگاه علوم پزشکی ایبادان ابادان ، نیجریه
5 - گروه گیاهشناسی دانشگاه ابادانان ابادان ، نیجریه
6 - گروه محیط زیست ،دانشکده علوم ، مهندسی و آمپر؛ فناوری ، دانشگاه جنوبی تگزاس ، هیوستون ، ایالات متحده
7 - گروه علوم زیست پزشکی
دانشگاه صنعتی شبه جزیره کیپ
بلزویل ، آفریقای جنوبی.
8 - گروه فارماکولوژی دامپزشکی و سم شناسی دانشکده دامپزشکی دانشگاه ابادان
الکلمات المفتاحية: Oxidative stress, Diabetes, Immunohistochemistry, Cardiotoxicity, Momordica charantia,
ملخص المقالة :
Background & Aim:Momordica charantiais an extensively distributed plant that is broadly prescribed in African medical system for treatment of various ailments. The plant has a comprehensive range of therapeutic uses. The present study reveals the antioxidative and cardioprotective abilities of methanol leaf extract of Momordica charantia (MLEMC) against cardiotoxicity using alloxan-induced animal model. Experimental: The ameliorative effect of the methanol leaf extract of Momordica charantia (MLEMC) was studied in alloxan-induced cardiac injury in 50 rats divided into five groups (A-E) (n =10) i.e. group A control, group B was toxicant group, group C animals received glibenclamide treatment while groups D and E received extracts at 200 and 400 mg/kg doses, respectively, for 28 days. Histopathological changes, serum cardiac injury markers such as myeloperoxidase (MPO) activity, nitric oxide (NO) contents; oxidative status, blood pressure, electrocardiogram, cardiac P38 and CRP were evaluated. Results: The extract-treated group showed a decreased level of oxidant markers such as malondialdehyde(MDA) and hydrogen peroxide(H2O2) but increased levels of protein thiols, non-protein thiols, glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. Heart sections revealed mild distortion of the cardiac architecture compared to toxicant group while decreased expression of cardiac P38 and CRP in extract-treated groups was observed. Recommended applications/industries: The plant extract exhibited anti-oxidant and anti-inflammatory effects, thereby displaying cardio-protective property which propose the plant as a good natural source for herbal nutraceuticals.
Abubaker, S., Shanmukha, I., Jyoti, T.M. and Gupt, K. 2012. Cardioprotective effect of Spathodea campanulata bark on isoproterenol induced myocardial infarction in rats. Asian Pacific Journal of Tropical Diseases, 2:S1–S5.
Amjad, A.K., Mohammed, A.A. and Arshad, H.R. 2018. Myeloperoxidase as an Active Disease
Biomarker: Recent Biochemical and Pathological Perspectives. Medical Sciences (Basel), 6(2): 33.
Buetler, E., Duron, O., Kelly, B.M. 1963. Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61:882-888.
Chand, K., Bhatnagar, V., Agarwala, S., Srinivas, M., Das, N. and Singh, M.K. 2015. The incidence of portal hypertension in children with choledochal cyst and the correlation of nitric oxidelevels in the peripheral blood with portal pressure and liver histology. Journal of Indian Association of Pediatric Surgeons 20: 133–138.
Cristina, M.S., Ana, M.P. andRaquel, S. 2013. Endothelial dysfunction - A major mediator of diabetic vascular disease. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease,1832(1): 2216-2231.
Drury, R.A., Wallington, E.A. and Cancerson, R. 1976. Carlton’s histopathological techniques. Oxford: Oxford University press, 279-280.
Gjin, N. 2019. Myeloperoxidase – A bridge linking inflammation and oxidative stress with cardiovascular disease. Clinica Chimica Acta, 493: 36-51.
Habig, W.H., Pabst, M.J. and Jakoby, W.B. 1974. Glutathione-S-transferases. The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249 (22): 7130-7139.
Hiroyuki, T., Tomomi, I., Shunji, H., Nobuhiro, S., Hideo, U., Ryo, N., Kensuke, E. andAkira, T. 2001. Greater susceptibility of failing cardiac myocytes to oxygen free radical-mediated injury. Cardiovascular Research, 49(1): 103–109.
Holditch, S.J., Schreiber, C.A., Nini, R., Tonne, J.M., Peng, K.W. and Geurts, A. 2015. B-Type natriuretic peptide deletion leads to progressive hypertension, associated organ damage, and reduced survival: novel model for human hypertension. Hypertension, 66:199-210.
Jonathan, A.S. and Paul, B.R. 2011The role of store-operated calcium influx in skeletal muscle signalling.Cell Calcium, 49(5): 341-349.
Kruidenier, L., Kuiper, I., Van Duijn, W., Mieremet-Ooms, M.A., van Hogezand, R.A., Lamers, C.B. and Verspaget, H.W. 2003. Imbalanced secondary mucosal antioxidant response in inflammatory bowel disease. Journal of Pathology, 201(1):17-27.
Kutter, D., Devaquet, P., Vanderstocken, G., Paulus, J.M., Marchal, V. and Gothot, A. 2000. Consequences of total and subtotal myeloperoxidase deficiency: risk or benefit? Acta Haematologica, 104:10e5.
Misra, H.P. and Friedovich I. 1972. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for super oxide dismutase. Journal of Biological Chemistry, 24(10): 3170-3175.
Moron, M.S., De Pierre, J.N. and Mannervik, V. 1979. Levels of glutathione, glutathione reductase, glutathione-S-transferase in rat lung and liver. Biochimica et Biophysica. Acta, 582:67-68.
Moskovitz, J.B., Hayes, B.D., Martinez, J.P., Mattu, A. and Brady, W.J. 2013. Electrocardiographic implications of the prolonged QT interval. American Journal of Emergency Medicine, 31: 866–871.
Nathaniel, T., Ghassan, J.M., Jihan, T., Imran, R., Antony, K.L. and Roland, S. 2017. The roles of myeloperoxidase in coronary artery disease and its potential implication in plaque rupture. Redox Report, 22(2): 51-73.
National Institute of Health, NIH. 1985. Guide for the care and use of Laboratory Animals U.S.Department of Health Education and welfare.
Naziroglu, M., Butterworth, J.P. and Sonmez, T.T. 2011. Dietary vitamin C and E modulate antioxidant levels in blood, brain, liver, muscle and testes in diabetic aged rats. International Journal for Vitamin and Nutrition Research, 81: 347-357.
Normann, S.J., Priest, R.E. and Benditt, E.P. 1961. Electrocardiogram in the normal rat and its alteration with experimental coronary occlusion. Circulation Research, 9: 282-287.
Ojha, S., Bhatia, J., Arora, S., Golechha, M., Kumari, S. and Arya, D.S. 2011. “Cardioprotective effects of Commiphora mukul against isoprenaline-induced cardiotoxicity: a biochemical and histopathological evaluation,” Journal of Environmental Biology, 32 (6): 731–738.
Olaleye, S.B., Adaramoye, O.A., Erigbali, P.P. and Adeniyi, O.S. 2007. Lead exposure increases oxidative stress in the gastric mucosa of HCl/ethanol-exposed rats. World Journal of Gastroenterology, 13: 5121–5126.
Opara, E.C. 2002. Oxidative stress, micronutrients, diabetes mellitus and its complications.Journal of the Royal Society for the Promotion of Health, 122: 28-34.
Oyagbemi, A.A., Omobowale, T.O., Akinrinde, A.S., Saba, A.B., Ogunpolu, B.S. and Daramola,O. 2015. Lack of reversal of oxidative damage in renal tissues of lead acetate-treated rats. Environmental Toxicology, 30(11): 1235–1243.
Patel, N., Raval, S., Goriya, H., Jhala, M. and Joshi, B. 2007. Evaluation of antidiabetic activity of Coldenia procumbens in alloxan-induced diabetes in rat. Journal of Herbal Pharmacotherapy, 7:13-23.
Phillips, L., Toledo, A.H., Lopez-Neblina, F., Anaya-Prador T. and Pereyra, L.H. 2009. Nitric oxide mechanism of protection in ischaemia and reperfusion injury. Journal of Investigative Surgery, 22(1): 46-55.
Richard, C.J. and Joseph, L. 2010. Vascular nitric oxide: formation and function. Journal of Blood Medicine, 1:147-162
Redmond, C. and Colton, T. 2001. "Clinical significance versus statistical significance". Biostatistics in Clinical Trials. Wiley Reference Series in Biostatistics (3rd ed.). West Sussex, United Kingdom: John Wiley & Sons Ltd, pp. 35–36.
Sabeena Farvin, K.H., Anandan, R., Kumar, S.H.S., Shiny, K.S, Sankar, T.V., Thankappan, T.K. 2004. Effect of squalene on tissue defence system in isoproterenol-induced myocardialinfarction in rats. Pharmacological Research, 50(3): 231–236.
Sedlak, J. and Lindsay, R.H. 1968. Estimation of total protein-bound and non-protein sulfhydryl groups in tissue with Ellman’s reagent. Analytical Biochemistry, 25: 1192 -1205.
Sharma, N. and Kar, A. 2014. Combined effects of Gymnema sylvestre and Glibenclamide on alloxan-induced diabetic mice. International Journal of Applied Pharmaceutics 6(2): 11-14.
Solano, M.P. and Goldberg, R.B. 2006. Management of dyslipidaemia in diabetes. Cardiology Review, 14:125-135.
Taiye, R.F., Blessing, U., Ademola, A.O., Temidayo, O.O. and Temitayo, O.A. 2016. Antidiabetic and antioxidant effects of Croton lobatus L. in alloxan-induced diabetic rats. Journal of Ethnopharmacology, 5(4): 364-371.
Thirumalai, T., Viviyan Therasa, S., Elumalai, E.K. and David, E. 2011. Hypoglycemic effect of Brassica juncea (seeds) on streptozotocin induced diabetic male albino rat. Asian Pacific Journal of Tropical Biomedicine 1 (4): 323-325.
Tshepiso, J.M., Esameldin, E.E., Lyndy, J.M., Luc, V. and Jacobus, N.E. 2016. The correlation between antimutagenic activity and total phenolic contents of extracts of 31 plant species with high antioxidant activity. BMC Complementary and Alternative Medicine, 16: 490.
Vannimwegen, C., Vaneijnsbergen, B., Boter, J. and Mullink, W. 1973. A Simple Device for indirect measurement of blood pressure in mice. Laboratory Animals, 7:73-84.
Varshney, R. and Kale, R.K. 1990. Effect of Calmodulin antagonist on radiation induced lipid peroxidation in microsomes. International Journal of Radiation Biology, 58:733-743.
Viswanatha, G.L.S., Vaidya, S.K., Ramesh, C., Krishnadas, N., Rangappa, S. 2010. Antioxidant and antimutagenic activities of bark extract of Terminalia arjuna. Asian Pacific Journal of Tropical Medicine, 3: 965-970.
Wehash, F.E., Abpo-Ghanema II, Saleh, R.M. 2012. Some physiological effects of Momordica charantia and Trigonella foenum-graecum extracts in diabetic rats as compared with cidophage®. World Academy of Science, Engineering and Technology, 64: 1206-1214.
Witchel, H.J. and Hancox, J.C. 2000. Familial and acquired long QT syndrome and the cardiac rapid delayed rectifier potassium current. Clinical and Experimental Pharmacology and Physiology, 27:753–766.
Wolff, S.F. 1994. Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydrogen peroxides. Methods in Enzymology, 233:182-189.
Wu, L., Chen, Y., Gao, H., Yin, J. and Huang, L. 2016. Cadmium-induced cell killing in Sacharomyces cerevisiae involves increases in intracellular NO levels. FEMS Microbiology Letters, 363(6).
Yibchok-Anun, S., Adisakwattana, S., Yao, C.Y., Sangvanich, P., Roengsumran, S., Hsu, W.H. 2006. Slow acting protein extract from fruit pulp of Momordica charantia with insulin secretagogue and insulinomimetic activities. Biological and Pharmaceutical Bulletin, 29:1126–1131.
Zhang, Z., Li, G., Szeto, S.S., Chong, C., Quan, Q., Huang, C., Cui, W., Guo, B., Wang, Y., Han, Y., Michael Siu, K.W., Lee, S.M., Chu, I.K. 2015. Examining the neuroprotective effects of protocatachuic acid and chrysin on in vitro and in vivo models of Parkinson’s disease. Free Radical Biology and Medicine, 84: 331-343.
