The effect of different concentrations of BAP on micropropagation of two species of Paneer-booti (Withania coagulans L.) and (Withania somnifera L.)
الموضوعات : مجله گیاهان دارویی
فرشته نورالهی
1
(کارشناسی ارشد، دانشکده کشاورزی، دانشگاه آزاد اسلامی، واحد شیروان، شیروان، ایران;)
ابراهیم گنجی
2
(مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی، گروه تحقیقات علوم زراعی و باغی، AREEO، مشهد، ایران)
الکلمات المفتاحية: Growth Regulator, Branch number, Branching, Propagation rate,
ملخص المقالة :
Background & Aim: Withania genus has more than 200 species, of which two important species (W. somnifera) and (W. coagulans) have high medicinal value and their rapid and extensive reproduction with new methods is very important to expand the area of cultivation. Propagation of crops in vitro is an accessible method for mass propagation. Therefore, the selection of a suitable culture medium is necessary to motivate shoot regeneration from explants and proliferation. The main purpose of this study was to present a rapid and efficient method for mass propagation of virus-free and disease-free plants under in vitro culture conditions.Experimental: The experiment was designed in a completely randomized design (CRD) with three replicatesin. The seeds were sterilized and placed on MS medium after being collected from their natural habitat in Sistan and Baluchestan province. It was then cultured on MS medium containing benzyl amino purine growth regulator at four levels of 0, 0.5, 1 and 1.5 mg/l for branching.Results: The results showed that in coagulans specie in MS medium containing 0.5 mg/l of benzyl amino purine (BAP) with an average proliferation of 3.66 of shoots and a length of 2.85 cm, the highest branching was obtained. Also, the highest propagation coefficient and number of leaves were obtained in benzyl amino purine (BAP) treatment with a concentration of 0.5 mg/l. The lowest propagation rate was reported in control (without hormones). In this experiment, application of benzyl amino purine at concentrations of 0.5 and 1 mg/l improved seedling quality which was more effective in coagulans and contained higher quality and fresher seedlings. Recommended applications/industries: In general, for the production of seedlings with high branching percentages, the use of benzyl amino purine at a concentration of 0.5 mg/l is recommended for optimal branching.
Arora, S., Dhillon, S., Rani, G. and Nagpal, A. 2004. The in vitro antibacterial/synergistic activities of Withania somnifera extracts. Fitoterapia, 75(3-4):385-388.
Bagheri, A., Ziaratnia, M. and Hosseini, M. 2005. In vitro culture of trees. Ferdowsi University of Mashhad. (In Farsi)
Ghorbani Ghozhdi, H. 2014. An Introduction to the basics of herbs, spices, and aromatic plants. The University of Shahroud Publication. pp. 512.
Singh, D. and Dixit, V.K. 2008. Dual Elicitation for improved production of withaferin A by cell suspension cultures of Withania somnifera. Applied Biochemistry and Biotechnology, 151(2-3):556-564.
Beena, M.R., Martin, K.P., Kirti, P.B. and Hariharan, M. 2000. Rapid in vitro propagation of medicinally important Ceropegia candelabrum L. Plant Cell, Tissue and Organ Culture, 72: 285- 288.
Gupta, S.K., Khanuja, S., Kumar, S. 2001. In vitro micropropagation of Lippia alba. Current Science. 81: 206-210.
Gupta, V. and Keshari, B.B. 2013. Withania coagulans Dunal. (Paneer doda): A review. International Journal of Ayurvedic and Herbal Medicine, 3(5): 1330-1336.
Hussain, A., Qarshi, I.A., Nazir, H. and Ullah, I. 2012. Plant tissue culture: current status and opportunities, In: A. Leva and L. M. R., Rinaldi (eds). Recent Advances in plant in vitro culture. INTECH, pp. 1-28.
Hosseini, R., Lamers, G.E., Soltani, H.M., Meijer, A.H., Spaink, H.P. and Schaaf, M.J. 2016. Efferocytosis and extrusion of leukocytes determine the progression of early mycobacterial pathogenesis. Journal of Cell Science, 129(18):85-95.
Kaykha, Z., Valizadeh, M., Valizadeh, J. and Taheri, KH. 2017. Studying the quantity and quality of fatty acids in the seeds of Withania coagulans (Stocks) Dun. and Withania somnifera (L.) Dun. collected from different habitats of Sistan and Baluchestan. Iranian Journal of Medicinal and Aromatic Plants, 33(5): 730-740.
Khan, N., Alam, M.S. and Nath, UK. 2004. In vitro regeneration of garlic through callus culture. Journal of Biological Sciences, 4: 189-191.
Liao, Z., Chen, M., Tan, F., Sun, X. and Tang, K. 2004. Micropropagation of endangered aloe Chinese. Plant Cell Tissue and Organ Culture, 76 (1): 83-86.
Nahok, A. (2013). Tissue culture and micropropagation of Withania somnifera. Ph.D. Thesis. Faculty of Agriculture Zabol University, Iran. (in Farsi)
Nathiya, S., Pradeepa, D., Devasena, T. and Senthil, K. 2013. Studies on the effect of sucrose, light, and hormones on micropropagation and in vitro flowering of Withania somnifera var. Jawahar-20. The Journal of Animal and Plant Sciences, 23(5): 1391-1397.
Nikolic, R., Mitić, N. and Nešković, M. 1997. Evaluation of agronomic traits in tissue culture–derived progeny of bird's-foot trefoil. Plant Cell, Tissue and Organ Culture, 48(1): 67-69.
Pashmforosh, N. and Ahmadabadi, M. 2016. In vitro regeneration of red nightshade from different explants andevaluation of gene transfer using a biolistic gun. Genetic and Biomechanical Engineering, 5(2): 167-177. (In Farsi)
Prathanturarug, S., Soonthornchareonnon, N., Chuakul, W., Phaidee, Y. and Saralamp, P. 2003. High-frequency shoot multiplication in Curcuma longa L. using thidiazuron. Plant Cell Reports, 21: 1054–1059.
Sajc, L., Grubisic, D. and Vunjak-Novakovic, G. 2000. Bioreactors for plant engineering: anoutlook for further research. Journal Biochemistry Engineering, 4: 89–99.
Sanjida, RM., Sarker, R.H. and Hoque, M.I. 2011. In vitro plant regeneration in Brassica spp. Plant Tissue Culture and Biotechnology, 21: 127-134.
Supe, U., Dhote, F. and Roymon, M.G. 2006. In vitro plant regeneration of Withania somnifera. Plant Tissue Culture and Biotechnology, 16(2): 111-115.
Valizade, M., Bagheri, A., Valizadeh, J., Mirjalili, MH. and Moshtaghi, N. 2015. Phytochemical investigation of Withania coagulans (Stocks) Dunal in natural habits of Sistan and Baluchestan province of Iran. Iranian Journal of Medicinal and Aromatic Plants. 31(3): 406-417.
Yang, H., Shi, G. and Dou, Q.P. 2007. The tumor proteasome is a primary target for the natural anticancer compound withaferin A isolated from ‘‘Indian Winter Cherry’’. Molecular Pharmacology, 71(2): 426-437.
Zhang, X., Samadi, A.K., Roby, K.F., Timmermann, B. and Cohen, M.S. 2012.Inhibition of cell growthand induction of apoptosis in ovarian carcinoma cell lines CaOV3 and SKOV3 by natural withanolide Withaferin A. Gynecologic Oncology, 124(3): 606-612.
