Memory-enhancing potentials of hydroalcoholic extract of Eragrostis tremula Hochst. exSteud. (Poaceae) in mice
الموضوعات : مجله گیاهان دارویی
عبداله نظیفی
1
,
محمد گربا
2
,
موسی علیو
3
,
نوحو محمد دانجوما
4
1 - گروه فارماکولوژی و درمانی ، دانشکده علوم دارویی ، دانشگاه Bayero ، کانو ، نیجریه.
2 - گروه فارماکولوژی و درمان ، دانشگاه احمدو بلو ، نیجریه.
3 - گروه فارماکولوژی و درمانی ، دانشکده علوم دارویی ، دانشگاه Bayero ، کانو ، نیجریه.
4 - گروه داروسازی و درمانی ، دانشکده علوم دارویی ، دانشگاه احمدو بلو ، زریا-نیجریه
الکلمات المفتاحية: Learning, Memory, exploration, Amnesia, Eragrostis tremula,
ملخص المقالة :
Background & Aim:Cognitive impairment is one of the age-related mental problems and a typical indicator of neurodegeneration. Eragrostis tremula Hochst. ex Steud. is a commonly used medicinal plant in Nigeria for memory enhancement. This study, therefore, aimed at evaluating the memory-enhancing potential of aqueous ethanolic extract of E. tremula in mice. Experimental: Classes of phytochemicals present in the extract were determined using standard protocol while its oral median lethal dose (LD50) in mice was estimated. The effect of E. tremula extract (125, 250 and 500 mg/kg) on learning and memory was evaluated in mice using behavioural paradigms: elevated plus maze (EPM), novel object recognition and Barnes maze. Open field and hole-board tests were also carried out to evaluate locomotion. Results: The phytochemical constituents of E. tremula were alkaloids, cardiac glycosides, flavonoids, tannins, saponins, steroids and triterpenes. Oral LD50 was estimated to be >5000 mg/kg. E. tremula extract significantly (P<0.05) decreased the transfer latency of mice during the retention phase of EPM test. In the novel object recognition test, it significantly (P<0.05) increased the discrimination index. In Barnes maze test, the extract significantly (P<0.05) decreased the mean primary errors during the acquisition trials. It also significantly (P<0.05) decreased the primary latency, primary error and increased the time spent in the target quadrant during the probe trial. E. tremula extract significantly (P<0.05) decreased the immobility time of mice in an open field at 250 mg/kg, while in the hole-board test, it significantly (P<0.05) increased the mean head-dip of mice at 125 mg/kg when compared to the negative control. Recommended applications/industries: The ethanol extract of E. tremula possesses memory enhancing properties which can be utilized in the management of amnesia and cognitive deficit.
Akobundu, I.O. and Agyakwa, C.W. 1998. A handbook of West African Weeds. International Institute of Tropical Agriculture, Ibadan, Nigeria. p. 486.
Anand, R., Gill, K.D. and Mahdi, A.A. 2014. Therapeutics of Alzheimer’s disease: past, present and future. Neuropharmacology, 76: 27-50.
Attar, A., Liu, T., Chan, W.T.C., Hayes, J., Nejad, M., Lei, K. and Bitan, G. 2013. A shortened Barnes maze protocol reveals memory deficits at 4-months of age in the triple-transgenic mouse model of Alzheimer’s disease. PLoS ONE, 8(11): 1-9.
Barker, G.R.I., Banks, P.J., Scott, H., Ralph, G.S., Mitrophanous, K.A., Wong, L.F., Bashir, Z.I., Uney, J.B. and Warburton, E.C. 2017. Separate elements of episodic memory subserved by distinct hippocampal-prefrontal connections. Nature Neuroscience. 20: 242-250.
Bhanumathy, M., Harish, M.S., Shivaprasad, H.N. and Sushma, G. 2010. Nootropic activity of Celastrus paniculatus seed. Pharmaceutical Biology, 48(3): 324-327.
Bhattacharjee, A., Shashidhara, S.C. and Saha, S. 2015. Nootropic activity of Crataeva nurvala Buch-Ham against scopolamine induced cognitive impairment. EXCLI Journal, 14: 335-345.
Brown, R.E., Corey, S.C. and Moore, A.K. 1999. Differences in measure of exploration and fear in mhc-congenic C57BL/6J and B6-H-2K Mice. Behaviour Genetics, 26: 263-271.
Chen, Z., Xu, A.J., Li, R. and Wei, E.Q. 2002. Reversal of scopolamine-induced spatial memory deficits in rats by TAK-147. Acta Pharmacologica Sinica, 23(4): 355-360.
Colerangle, J.B. 2017. Preclinical development of nononcogenic drugs (Small and large molecules). (2nd ed). In: Faqi, A.S. (Ed). A Comprehensive Guide to Toxicology in Nonclinical Drug Development.Academic Press, U.K. pp. 659-683.
Cummings, J., Lee, G., Mortsdorf, T., Ritter, A. and Zhong, K. 2018. Alzheimer’s disease drug development pipeline: 2017. Alzheimer’s and Dementia: Translational Research and Clinical Interventions, 3: 367-384.
Dhingra, D., Parle, M. and Kulkarni, S.K. (2004). Memory enhancing activity of Glycyrrhiza glabra in mice. Journal of Ethnopharmacology, 91: 361-365.
Ennaceur, A. 2010. One-trial object recognition in rats and mice: methodological and theoretical issues. Behavioural Brain Research, 215: 244-254.
Evans, W.C. 2009. Trease and Evans’ Pharmacognosy. (16th ed). Elsevier Health Sciences, London, U.K.
File, S. and Wardill, A.G. 1975. Validity of head-dipping as a measure of explorating a modified hole-board. Psychopharmacologia, 44: 53-59.
Gawel, K., Gibula, E.,Marszalek-Grabska, M., Filarowska, J. and Kotlinska, J.H. (2019). Assessment of spatial learning and memory in the Barnes maze task in rodents-methodological consideration. Naunyn-Schmiedeberg's Archives of Pharmacology, 392, 1-18.
George, A., Ng, C.P., O’Callaghan, M., Jensen, G.S. and Wong, H.J. 2014. In vitro and ex-vivo cellular antioxidant protection and cognitive enhancing effects of an extract of Polygonum minus Huds (Lineminus) demonstrated in Barnes maze animal model for memory and learning. BMC Complementary and Alternative Medicine, 14: 1-10.
Gulpers, B., Ramakers, I., Hamel, R., Köhler, S., Voshaar, R.O. and Verhey, F. 2017. Anxiety as a Predictor for Cognitive Decline and Dementia: A Systematic Review and Meta-Analysis. The American Journal of Geriatric Psychiatry, 24(10): 823-842.
Harrison, F.E., Hosseini, A.H. and MacDonald, M.P. 2009. Endogenous anxiety and stress responses in water maze and Barnes maze spatial memory tasks. Behavioral Brain Research, 198: 247-251.
Itoh, J., Nabeshima, T. and Kameyama, T. 1990. Utility of an elevated plus maze for the evaluation of nootropics, scopolamine and electroconvulsive shock. Psychopharmacology, 101: 27-33.
Jung, W.Y., Kim, H., Jeon, S.J., Park, H.J., Choi, H.J., Kim, N.J., Kim, D.H., Jang, D.S. and Ryu, J.H. 2018. Eclalbasaponin II ameliorates the cognitive impairment induced by cholinergic blockade in mice. Neurochemical Research, 43(2): 351-362.
Komada, M., Takao, K. and Miyakawa, T. 2008. Elevated Plus Maze for Mice. Journal of Visualized Experiments, 22: e1088.
Konrath, E.L., Neves, B.M., Lunardi, P.S., Passos, C.S., Simões-Pires, A., Ortega, M.G., Goncalves¸ C.A., Cabrera, J.L., Moreira, J.C.F. and Henriques A.T. 2012. Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from South America on alkaloid extracts. Journal of Ethnopharmacology, 139: 58-67.
Liu, X., Zhang, Y., Li, J., Wang, D., Wu, Y., Lu, Z., Yu, S.C.T., Li, R. and Yang, X. 2014. Cognitive deficits and decreased locomotor activity induced by single-walled carbon nanotubes and neuroprotective effects of ascorbic acid. International Journal of Nanomedicine, 9: 823-839.
Loomis, T.A. and Hayes, A.W. 1996. Loomis’s essentials of toxicology. 4th edition, California, Academic press. pp. 17-32.
Lorke, D. 1983. A new approach to practical acute toxicity testing, Archives of Toxicology. 54: 275-287.
Lueptow, L.M. 2017. Novel object recognition test for the investigation of learning and memory in mice. Journal of Visualized Experiments. 126: e55718.
Lukasik, K.M., Waris, O., Soveri, A., Lehtonen, M. and Laine, M. 2019. The relationship of anxiety, stress anddepressive symptoms with working memory per-formance in a large non-depressed sample. Frontiers in Psychology,10(4): 1-9.
Mahmud, B., Shehu, S., Sani, M.Y. and Magaji, M.G. 2019. Methanol stem extract of Parquetinanigrescens(Asclepiadaceae) possesses memory-enhancing potential in acute mice models of cognition. Journal of Herbal Drugs, 9(4): 197-205.
Na, C.S., Hong, S.S., Choi, Y., Lee, Y.H., Hong, S.H., Lim, J., Kang, B.H., Park, S. and Lee, D. 2010.Neuroprotective effects of constituents of Eragrostis ferrugineaagainst Aβ-induced toxicity in PC12 cells. Archives of Pharmacal Research, 33(7): 999-1003. doi: 10.1007/s12272-010-0704-5
Na, C.S., Lee, M.J., Hong, S.S., Choi, Y., Lee, J., Park, S., Lee, Y.H. and Hong, S.H. (2018). Antioxidant and neuroprotective activity of the aerial parts of seven Eragrostis species and bioactive compounds from E. japonica. Records of Natural Products, 12(1): 101-106.
O’Leary, T.P. and Brown, R.E. 2013. Optimization of apparatus design and behavioral measures for the assessment of visuo-spatial learningand memory of mice on the Barnes maze. Learning and Memory, 20: 85-96.
Odugbemi, T. 2008. Outlines and pictures of medicinal plants from Nigeria. University of Lagos Press, Nigeria. pp. 73-117.
Patterson, C., Lynch, C., Bliss, A., Lefevre, M. and Weidner, M., 2018. World AlzheimerReport 2018 the State of the Art of Dementia Research: New Frontiers. Alzheimer’sDisease International (ADI), London, pp. 1-42.
Peterson, P.M. and Vega, I.S. 2007. Eragrostis (Poaceae: Chloridoideae: Eragrostideae: Eragrostidinae) of Peru. Annals of the Missouri Botanical Garden, 94(4): 745-790.
Poilecot, P., Timberlake, J., Drummond, R.B., Mapaura, A. and Shava, S. 2007. Eragrostis species of Zimbabwe. Editions Quea. pp. 193.
Rather, M.A., Justin-Thenmozhi, A., Manivasagam, T., Saravanababu, C., Guillemin, G.J. and Essa, M.M. 2019. Asiatic acid attenuated aluminum chloride-induced tau pathology, oxidative stress and apoptosis via AKT/GSK-3β Signaling Pathway in Wistar Rats. Neurotoxicity Research, 35(4): 955-968.
Sandry, J. 2015. Working memory and memory loss in neurodegenerative disease. Neurodegenerative Disease Management, 5(1): 1-4.
Silva, T., Reis, J., Teixeira, J. and Borges, F. 2014. Alzheimer's disease, enzyme targets and drug discovery struggles: from natural products to drug prototypes. Ageing Research Reviews, 15: 116-145.
Soladoye, M.O., Osipitan, A.A., Sonibare, M.A. and Chukwuma, E.C. 2010. From ‘Vagabonds’ to Ethnobotanical Relevance: Weeds of the Campus Sites of Olabisi Onabanjo University, Ago-Iwoye, Nigeria. Ethnobotanical Leaflets, 14: 546-558.
Srivastava, A., Srivastava, P., Pandey, A., Khanna, V.K. and Pant, A.B. 2019. Phytomedicine: A potential alternative medicine in controlling neurological disorders. In: Khan, M.S.A, Ahmad, I. and Chattopadhyay, D. (Eds.) New look to phytomedicine, Academic press, India. pp. 625-655.
Szentes, N., Tékus, V., Mohos, V., Borbély, E. and Helyes, Z. 2019. Exploratory and locomotor activity, learning and memory functions in somatostatin receptor subtype 4 gene-deficient mice in relation to aging and sex. GeroScience, 41(5): 631-641.
Tewari, D., Stankiewicz, A.M., Mocan, A., Sah, A.N., Tzvetkov, N.T., Huminiecki, L., Horban´ czuk, J.O. and Atanasov, A.G. 2018. Ethnopharmacological approaches for dementia therapy and significance of natural products and herbal drugs. Frontiers in Aging Neuroscience, 10(3): 1-24.
Thies, W. and Bleiler, L. 2013. Alzheimer’s disease: Facts and figures. Alzheimers Dementia. 9: 208-215.
Uddin, S., Al Mamun, A., Kabir, T., Jakaria, M., Mathew, B., Barreto, G.E. and Ashraf, G. 2018. Nootropic and Anti-Alzheimer’s Actions of Medicinal Plants: Molecular Insight into Therapeutic Potential to Alleviate Alzheimer’s Neuropathology. Molecular Neurobiology, 56(7): 4925-4944. doi: 10.1007/s12035-018-1420-2
Vyas, S., Kothari, S.L. and Kachhwaha, S. 2019. Nootropic medicinal plants: Therapeutic alternatives for Alzheimer’s disease. Journal of Herbal Medicine, 17-18: 100291.
Warburton, E.C. 2018. Using the spontaneous object recognition memory tasks to uncover the neural circuitry of recognition memory: The importance of thalamic nuclei. In: Ennaceur, A. and De Souza Silva, M.A. (Eds). Handbook of object novelty recognition. Academic press, UK, pp. 197-203.
Yaro, A.H., Muhammad, M.A., Nazifi, A.B. and Magaji, M.G. 2015. Butanol soluble fractions of Cissus cornifoliamethanolic leaf extract and behavioral effects in mice. The Journal of Phytopharmacology, 4(4): 202-206.
You, Y.X., Shahar S., Haron, H. and Yahya, H.M. 2018. More Ulamfor your brain: A review on the potential role of Ulamin protecting against cognitive Decline. SainsMalaysiana, 47(11): 2713-2729.
