مقایسه فعالیت ضد میکروبی نانوذرات اکسید روی بیوسنتز شده با عصاره گیاه بن سرخ و سنتز شده شیمیایی
محورهای موضوعی : میکروبیولوژی
سیده معصومه میرنوراللهی
1
*
,
رامین محمدی آلوچه
2
,
پریا علیزاده عوری
3
1 - استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد تهران مرکزی، ایران
2 - استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد تهران مرکزی، ایران
3 - کارشناسی ارشد،گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد تهران مرکزی
کلید واژه: سنتز سبز, نانوذرات اکسید روی, عصاره برگ گیاه بن سرخ, استرپتوکوکوس اینیه, آئروموناس هیدروفیلا ,
چکیده مقاله :
مقدمه: بیوسنتز نانوذرات به دليل استفاده از واکنشگرها وعوامل طبيعی نظير عصاره گياهان و مواد شيميایی غيرسمی، ریسک خطرپذیری برای انسان و اکوسيستم را به طور قابل توجهی کاهش می دهند. لذا در این پژوهش، عصاره آبی گیاه بن سرخ به عنوان عامل کاهنده و پایدار کننده جهت سنتز نانوذرات اکسید روی مورد استفاده قرار گرفت و سنتز شیمیایی نیز با هدف مقایسه فعالیت ضد میکروبی آنها بر ضد استرپتوکوکوس اینیه و آئروموناس هیدروفیلا انجام شد.
مواد و روش ها: پس از عصاره گیری، با افزودن غلظت 1 به 5 و 1 به 10 عصاره در برابر محلول اکسید روی mmol1، نانوذرات تشکیل شدند. آنالیز نانوذرات سنتز شده شیمیایی و گیاهی با دستگاه های UV-vis، XRD، TEM، FESEM، FTIR و Edax انجام و فعالیت ضد میکروبی آنها با روش انتشار از دیسک و چاهک وMIC بررسی شد.
نتایج: توليد نانوذرات سنتز شده با ثبت تغيير رنگ با اسپكتوفتومتر در محدودهnm 700-300 بررسی شد. بررسی با TEM نشان داد که شکل ذرات کروی و اندازه آنها nm 115-45 و دارای فعالیت ضد میکروبی بر ضد استرپتوکوکوس اینیه و آئروموناس هیدروفیلا بودند.
نتیجه گیری: نتایج نشان داد این گیاه تاثیر ضد میکروبی بر استرپتوکوکوس اینیه دارد. نانوذرات شیمیایی گر چه اثر بهتری نشان دادند اما تفاوت اثر بسیار ناچیز بوده و از آنجا که بیوسنتز نانومواد اثرات زیست محیطی بهتر و عوارض کمتری دارند، می توان گفت نانوذرات گیاهی می توانند جایگزین مطلوب برای نانوذرات شیمیایی و کاندید مناسبی برای جایگزینی آنتی بیوتیک درمانی در صنعت پرورش ماهی باشند.
Itroduction: Biosynthesis of nanoparticles due to the use of natural agents such as plant extracts and non-toxic chemicals significantly reduce the risk of danger to humans and the ecosystem. Therefore, in this research, the aqueous extract of the Allium jesdianum Extract was used as a reducing and stabilizing agent for the synthesis of zinc oxide nanoparticles, and the chemical synthesis was also carried out with the aim of comparing their antimicrobial activity against Streptococcus Iniae and Aeromonas Hydrophila. Materials and methods: After extracting, nanoparticles were formed by adding 1 to 5 and 1 to 10 concentrations of extracts against 1 mmol zinc oxide solution. Chemical and green synthesized were analyzed with UV-vis, XRD, TEM, FESEM, FTIR and Edax devices and their antimicrobial activity was investigated by disk, well diffusion method and MIC. Results: The production of synthesized nanoparticles was checked by recording the color change with a spectrophotometer in the range of 300-700 nm. Examination with TEM showed that the shape of the spherical particles and their size were 45-115 nm and they had antimicrobial activity against S. Iniae and A. Hydrophila. Conclusion: The results showed that this plant has an antimicrobial effect on Streptococcus iniae. Although chemical nanoparticles showed a better effect, the difference in effect is very small, and since the biosynthesis of nanomaterials has better environmental effects and fewer side effects, green nanoparticles can be a good substitute for chemical nanoparticles and a good candidate for replacing antibiotic therapy.
Matin F, Mirnurollahi SM, Behzadi R. Comparision and evaluation of the effect of disinfectants Microten, Savlon(strymid-c) and Banzalkonium cholorid on Escherichia coli. Iranian journal of biological. 2023;17(4):35-47.
Rai M, Kon K, Gade A, Ingle A, Nagaonkar D, Paralikar P, et al. Antibiotic resistance: can nanoparticles tackle the problem. 2016.
Yuvakkumar R, Suresh J, Nathanael AJ, Sundrarajan M, Hong SJMS, C E. Novel green synthetic strategy to prepare ZnO nanocrystals using rambutan (Nephelium lappaceum L.) peel extract and its antibacterial applications. 2014;41:17-27.
Ruddaraju LK, Pammi SVN, sankar Guntuku G, Padavala VS, Kolapalli VRMJAJoPS. A review on anti-bacterials to combat resistance: From ancient era of plants and metals to present and future perspectives of green nano technological combinations. 2020;15(1):42-59.
Shaikh S, Nazam N, Rizvi SMD, Ahmad K, Baig MH, Lee EJ, et al. Mechanistic insights into the antimicrobial actions of metallic nanoparticles and their implications for multidrug resistance. 2019;20(10):2468.
Adwan G, Abu-Shanab B, Adwan KJAPjotm. Antibacterial activities of some plant extracts alone and in combination with different antimicrobials against multidrug–resistant Pseudomonas aeruginosa strains. 2010;3(4):266-9.
Mobaiyen H, Jafari Sales A, Sayyahi JJJoABS. Evaluating antimicrobial effects of centaurea plant’s essential oil on pathogenic bacteria: staphylococcus aureus, staphylococcus epidermidis, and escherichia coli isolated from clinical specimens. 2015;5(4):479-87.
JAFARI-SALES A, Pashazadeh MJIJoLS, Biotechnology. Study of chemical composition and antimicrobial properties of Rosemary (Rosmarinus officinalis) essential oil on Staphylococcus aureus and Escherichia coli in vitro. 2020;3(1):62-9.
TABATABAEI YF, ALIZADEH BB, Zanganeh H. The comparison among antibacterial activity of Mespilus germanica extracts and number of common therapeutic antibiotics “in vitro”. 2015.
Raghunath A, Perumal EJIjoaa. Metal oxide nanoparticles as antimicrobial agents: a promise for the future. 2017;49(2):137-52.
10 Huh AJ, Kwon YJJJocr. “Nanoantibiotics”: a new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. 2011;156(2):128-45.
11 Raghupathi KR, Koodali RT, Manna ACJL. Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. 2011;27(7):4020-8.
Lemire JA, Harrison JJ, Turner RJJNRM. Antimicrobial activity of metals: mechanisms, molecular targets and applications. 2013;11(6):371-84.
Niño-Martínez N, Salas Orozco MF, Martínez-Castañón G-A, Torres Méndez F, Ruiz FJIjoms. Molecular mechanisms of bacterial resistance to metal and metal oxide nanoparticles. 2019;20(11):2808.
Brandelli A, Ritter AC, Veras FFJMnip. Antimicrobial activities of metal nanoparticles. 2017:337-63.
Saif S, Tahir A, Chen YJN. Green synthesis of iron nanoparticles and their environmental applications and implications. 2016;6(11):209.
Zhu X, Pathakoti K, Hwang H-M. Green synthesis of titanium dioxide and zinc oxide nanoparticles and their usage for antimicrobial applications and environmental remediation. Green synthesis, characterization and applications of nanoparticles: Elsevier; 2019. p. 223-63.
Kavoosi S, Yaghoubi HJC, Research M. Synthesis of silver nanoparticles using green method of plant extract european marjoram (Origanum majorana) and their antibacterial effects. 2017;30(2):161-73.
Stan M, Popa A, Toloman D, Silipas TD, Vodnar DC. Antibacterial and antioxidant activities of ZnO nanoparticles synthesized using extracts of Allium sativum, Rosmarinus officinalis and Ocimum basilicum. Acta Metall Sin (Engl. Lett.); 2016. 29(3): 228-36.
Elumalai K, Velmurugan SJASS. Green synthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from the leaf extract of Azadirachta indica (L.). 2015;345:329-36.
Gram L, Melchiorsen J, Bruhn JBJMb. Antibacterial activity of marine culturable bacteria collected from a global sampling of ocean surface waters and surface swabs of marine organisms. 2010;12:439-51.
Rasmussen-Ivey CR, Figueras MJ, McGarey D, Liles MRJFim. Virulence factors of Aeromonas hydrophila: in the wake of reclassification. 2016:1337.
Ahmed S, Chaudhry SA, Ikram S. A review on biogenic synthesis of ZnO nanoparticles using plant extracts and microbes: a prospect towards green chemistry. Journal of Photochemistry and Photobiology B: Biology. 2017;166:272-84.
Król A, Pomastowski P, Rafińska K, Railean-Plugaru V, Buszewski B. Zinc oxide nanoparticles: Synthesis, antiseptic activity and toxicity mechanism. Advances in colloid and interface science. 2017;249:37-52.
Deep G, Agarwal R. Antimetastatic efficacy of silibinin: molecular mechanisms and therapeutic potential against cancer. Cancer and Metastasis Reviews. 2010;29(3):447-63.
Riesenberg A, Kaspar H, Febler AT, Werckenthin C, Schwarz S. Susceptibility testing of Rhodococcus equi an interlaboratory test. Vet Microbiol 2016;194:30 -5
Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic AJIjon. Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study. 2012:6003-9.
Kim JS, Kuk E, Yu KN, Kim J-H, Park SJ, Lee HJ, et al. Antimicrobial effects of silver nanoparticles. 2007;3(1):95-101.
Khoshal Z, Vaziri A and Rahbarian R. Green production of silver nanoparticles from Eryngium planum and its antibacterial effect on Escherichia coli and Staphylococcus aureus. Iranian journal of biological sciences.2023 ;17(4):77-92.
28.Kaushikthakkar N, Snehitmhatre S, Raseshparikh Y. Biological synthesis of Metallic Nanoparticles. Nanomedicine 2010; 28:257 -62.
Azizinshermeh O , Valizadeh J , Noroozifar M, Ghasemi A, Valizadeh M. [Optimization, characterization and anti - microbial activity of gold nanoparticles biosynthesized using aqueous extract of Sambucus ebulus . L]. Phytochem J Med Plants 2016; 1:1 -18. (Persian)
Manokari M, Ravindran CP, Shekhawat MS. Production of zinc oxide nanoparticles using aqueous extracts of a medicinal plant Micrococca mercurialis (L.) Benth. WSN; 2016. 30:117.
. Ambika S, Sundrarajan M. Green biosynthesis of ZnO nanoparticles using Vitex negundo L. extract: spectroscopic investigation of interaction between ZnO nanoparticles and human serum albumin. J Photochem Photobiol; 2015. 149:143-8.
Bala N, Saha S, Chakraborty M, Maiti M , Das S , Basu Rand and Nandy P . Green synthesis of zinc oxide nanoparticles using Hibiscus subdariffa leaf extract: effect of temperature on synthesis, antibacterial activity and anti-diabetic activity. RSC Adv; 2015. 5(7):4993-5003.
Raj LFA, Jayalakshmy E. Biosynthesis and characterization of zinc oxide nanoparticles using root extract of zingiber officinale. Orient J Chem; 2015. 31(1):51-6.
Karnan T, Selvakumar SAS. Biosynthesis of ZnO nanoparticles using rambutan (Nephelium lappaceumL.) peel extract and their photocatalytic activity on methyl orange dye. J Mol Struct; 2016. 1125:358-6
Pantidos N, Horsfall LE. Biological synthesis of metallic nanoparticles by bacteria, fungi and plants. J Nanomed Nanotechnol. 2014;5(5):1 -10.
MohamedYM, Azzam AM, Amin BH, Safwat NA. Mycosynthesis of iron nanoparticles by Alternaria alternate and its antibacterial activity. African J of Biotechnol. 2015;14(4):1234 -41.
.Raut PS, Thorat V, Thakre R. Green Synthesis of Zinc Oxide (ZnO) Nanoparticles Using Ocimum Tenuiflorum Leaves. Int J Sci Res. 2015;17(5):1225 -8.
Yedurkar S, Maurya Ch, Mahanwar P. Biosynthesis of zinc oxide nanoparticles using ixora coccinea leaf extract—a green approach. Open J Synth Theory Appl. 2016;5(1):1 -14
Bhumi G, Savithramma N. Biological Synthesis of Zinc oxide Nanoparticles from Catharanthus roseus (l.) G. Don. Leaf extract and validation for antibacterial activity. Int J Drug Dev Res. 2014; 6(1):208 -14.
Neda Fazeli ASN, Seyed Amir Hossein Jalali, and Hojjatolah Zamani. Antimicrobial activity of extract derived from sea anemone (Stichodactyla haddoni) against Aeromonas hydrophila. Journal of Fisheries. 2021;74(1):85-96.
ALISHAHI M GM, NAJAFZADEH H, PASHMFOROOSH M. ANTIBACTERIAL EFFECTS OF SOME MEDICAL PLANT EXTRACTS ON AEROMONAS HYDROPHILA, YERSINIA RUCKERI AND STREPTOCOCCUS INIAE. SCIENTIFIC-RESEARCH IRANIAN VETERINARY JOURNAL. 2011;6(2):21-30.
Sivaraman SK, Elango I, Kumar S, Santhanam VJCS. A green protocol for room temperature synthesis of silver nanoparticles in seconds. 2009;97(7).
Sathyavathi R, Krishna MBM, Rao DNJJoN, Nanotechnology. Biosynthesis of silver nanoparticles using moringa oleifera leaf extract and its application to optical limiting. 2010;10:1-5.
Ahmad R, Khatoon N, Sardar MJASL. Antibacterial effect of green synthesized TiO2 nanoparticles. 2014;20(7-8):1616-20.
Dobrucka R, Długaszewska JJSjobs. Biosynthesis and antibacterial activity of ZnO nanoparticles using Trifolium pratense flower extract. 2016;23(4):517-23.
Ifeanyichukwu UL, Fayemi OE, Ateba CNJM. Green synthesis of zinc oxide nanoparticles from pomegranate (Punica granatum) extracts and characterization of their antibacterial activity. 2020;25(19):4521.
Janaki AC, Sailatha E, Gunasekaran SJSAPAM, Spectroscopy B. Synthesis, characteristics and antimicrobial activity of ZnO nanoparticles. 2015;144:17-22.
136. Chaudhary A, Kumar N, Kumar R, Salar RKJSAS. Antimicrobial activity of zinc oxide nanoparticles synthesized from Aloe vera peel extract. 2019;1:1-9.