بررسی تاثیرات تنش کم آبیبر پارامترهای فتوسنتزی در گیاه مریم گلیاز طریق روش JIP-تست

محورهای موضوعی : برگرفته از پایان نامه

ندا باغ عنایت ¹ , گیتی برزین ² , مجتبی جعفری نیا ³ , لیلا پیشکار ⁴ , ملیحه انتظاری ⁵

1 - گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد اسلامشهر، اسلامشهر، ایران
2 - گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد اسلامشهر، اسلامشهر، ایران
3 - گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد مرودشت، مرودشت، ایران
4 - گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد اسلامشهر، اسلامشهر، ایران
5 - گروه ژنتیک، دانشکده علوم و فناوری های نوین، واحد تهران پزشکی، دانشگاه آزاد اسلامی، تهران، ایران و مرکز تحقیقات علوم همگرای پزشکی فرهیختگان، بیمارستان فرهیختگان، واحد تهران پزشکی ، دانشگاه آزاد اسلامی، تهران، ایران

تاریخ دریافت : 1399/08/01 تاریخ پذیرش : 1400/06/10 تاریخ انتشار : 1401/02/01

کلید واژه: کم آبی, فلورسانس کلروفیل a, مریم گلی,

چکیده مقاله :

مقدمه:تنش کم آبی یکی از مهمترین تنش های غیر زیستی است که می تواند بر رشد و نمو گیاهان موثر باشد. گیاه مریم گلی نیز یکی از گیاهان دارویی مهم است که دارای خواص دارویی متعددی می باشد.
روش:در پژوهش حاضر تاثیرات سطوح متفاوت کم آبی شامل 25، 50، 75 و 100 درصد ظرفیت زراعی خاک بر روی پارامترهای فتوسنتزی در دو گونه حساس و مقاوم گیاه مریم گلی از طریق روش JIP- تست مورد بررسی قرار گرفت.
یافته ها:نتایج این تحقیق نشان داد که با افزایش تنش کم آبی از میزان مراکز فعال واکنش (RC/CS)، میزان انتقال الکترون در سیستم فتوسنتزی (ETo/CS)،کارایی واکنش های نوری در فتوسیستم II (φ_Po /(1- φ_Po ))، کارایی واکنش های بیوشیمیاییدر زنجیره انتقال الکترون (ψ_o /(1- ψ_o ))، کارایی رسیدن الکترون به فتوسیستم I(δ_Ro/(1- δ_Ro )) و شاخص کارایی انتقال الکترون از ابتدای فتوسیستم II تا انتهایI (PI total)کاسته شده است و میزان اتلاف انرژی در کلروفیل های آنتن (DIo/CS)افزایش یافته است.
نتیجه­گیری: نتایج این تحقیق نشان داد که تاثیرات منفی تنش کم آبی بر این پارامترها در گونه حساس مریم گلی (Salvia officinallis) بیش از گونه مقاوم (Salvia virgata) بوده است.

چکیده انگلیسی:

Water defficency stress is one of the most important abiotic stresses that can affect plant growth and development. Salvia is also one of the important medicinal plants that has several medicinal properties. In the present study, the effects of different levels of water deficincy including 25, 50, 75 and 100% of soil field capacity on photosynthetic parameters in two sensitive and resistant species of salvia were investigated by JIP-test method. The results of this study showed that with increasing water defficency stress the number of active reaction centers (RC / CS), the amount of electron transfer in the photosynthetic system (ETo / CS), the efficiency of light reactions in photosystem II (φPo / (-1 φPo)) , Efficiency of biochemical reactions in electron transfer chain (ψo / (1- ψo)), efficiency of electron delivery to photosystem I (δRo / (1- δRo)) and performance index of electron transfer from the beginning of photosystem II to the end of I (PI total) has been reduced and the amount of energy loss in antenna chlorophylls (DIo / CS) has increased. The results of this study also showed that the negative effects of water defficency stress on these parameters in the susceptible species of Salvia (Salvia officinallis) were more than the resistant species (Salvia virgata).

منابع و مأخذ:

1. Bansal, A. Thakur, S. Singh, and M. Bakshi, “Changes in crop physiology under drought stress : A review,” J. Pharmacognsy Phytochem., vol. 8, no. 4, pp. 1251–1253, 2019.


2. D. Costa, “Plant Resistance to Abiotic Stresses,” Plants, vol. 8, no. 553, pp. 10–13, 2019.


3. Barari and A. A. Branch, “Effect of Drought stress and its mechanism in plants,” Intwernational Jouranal Life Sci., vol. 10, no. 1, pp. 1–6, 2018, doi: 10.3126/ijls.v10i1.14509.


4. Yang, Y. Li, H. Chen, and J. Huang, “Photosynthetic Response Mechanism of Soil,” Plants, vol. 9, no. 363, pp. 1–15, 2020.


5. Ö. Arslan, A. S. B. Nalçaİyİ, Ş. Ç. Erdal, V. Pekcan, and Y. Kaya, “Analysis of drought response of sunflower inbred lines by chlorophyll a fluorescence induction kinetics,” Photosynthetica, vol. 58, no. S1, pp. 163–172, 2020, doi: 10.32615/ps.2019.171.


6. Jafarinia and M. Shariati, “Effects of salt stress on photosystem II of canola plant ( Barassica napus , L .) probing by chlorophyll a fluorescence measurements,” Iran. J. Sci. Technol., pp. 71–76, 2012.


7. Stavridou, R. J. Webster, and P. R. H. Robson, “Novel Miscanthus genotypes selected for different drought tolerance phenotypes show enhanced tolerance across combinations of salinity and drought treatments,” Ann. Bot., vol. 124, pp. 653–674, 2019, doi: 10.1093/aob/mcz009.


8. Oukarroum, S. El Madidi, G. Schansker, and R. J. Strasser, “Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering,” Environ. Exp. Bot., vol. 60, no. 3, pp. 438–446, Jul. 2007, doi: 10.1016/j.envexpbot.2007.01.002.


9. Karami, M., Hossini, S. E., Naghi Shahbi, M., Ebrahimzadeh, M. A. and Alemy, “Salvia limbata: Botanical, Chemical, Pharmacological and Therapeutic Effecte,” vol. 3, no. 1, pp. 108–127, 2015.


10. S. Reto J. Strasser, Merope Tsimilli- Michael, Analysis of the Fluorescence Transient Reto J . Strasser Merope Tsimilli- Michael Ministry of Education and Culture , Nicosia CY-1434 , Cyprus Alaka Srivastava, no. May 2014. 2004. doi: 10.1007/978-1-4020-3218-9.


11. Heber, V. Soni, and R. J. Strasser, “Photoprotection of reaction centers: thermal dissipation of absorbed light energy vs charge separation in lichens.,” Physiol. Plant., vol. 142, no. 1, pp. 65–78, May 2011, doi: 10.1111/j.1399-3054.2010.01417.x.


12. Heinrich Krause, S. Somersalo, E. Zumbusch, B. Weyers, and H. Laasch, “On the Mechanism of Photoinhibition in Chloroplasts. Relationship Between Changes in Fluorescence and Activity of Photosystem II,” J. Plant Physiol., vol. 136, no. 4, pp. 472–479, 1990, doi: 10.1016/S0176-1617(11)80038-6.


13. R. Falqueto, R. A. da Silva Júnior, M. T. G. Gomes, J. P. R. Martins, D. M. Silva, and F. L. Partelli, “Effects of drought stress on chlorophyll a fluorescence in two rubber tree clones,” Sci. Hortic. (Amsterdam)., vol. 224, no. June, pp. 238–243, 2017, doi: 10.1016/j.scienta.2017.06.019.


14. Dabrowski, A. H., Kalaji, H. M., Goltsev, V., & Piotr, “Exploration of Chlorophyll a Fluorescence and Plant Gas Exchange Parameters as Indicators of Drought Tolerance in Perennial Ryegrass,” sensors, vol. 19, pp. 27–36, 2019.


15. Rapacz, M. Wójcik-jagła, A. Fiust, and H. M. Kalaji, “Genome-Wide Associations of Chlorophyll Fluorescence OJIP Transient Parameters Connected With Soil Drought Response in Barley,” Front. Plant Sci., vol. 10, no. February, pp. 1–21, 2019, doi: 10.3389/fpls.2019.00078.


16. Mathur, S. I. Allakhverdiev, and A. Jajoo, “Analysis of high temperature stress on the dynamics of antenna size and reducing side heterogeneity of Photosystem II in wheat leaves (Triticum aestivum).,” Biochim. Biophys. Acta, vol. 1807, no. 1, pp. 22–9, Jan. 2011, doi: 10.1016/j.bbabio.2010.09.001.


17. M. Kalaji, A. Jajoo, A. Oukarroum, and M. Brestic, “Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions,” Acta Physiol. Plant., vol. 38, no. 102, pp. 1–11, 2016, doi: 10.1007/s11738-016-2113-y.


18. G. Choi and H. J. Jeong, “Comparison of Chlorophyll Fluorescence and Photosynthesis of Two Strawberry Cultivars in Response to Relative Humidity,” Horticutural Sci. Technol., vol. 38, no. 1, pp. 66–77, 2020.


19. Wang et al., “Effects of drought stress on photosynthesis and photosynthetic electron transport chain in young apple tree leaves,” Co. Biol. Biol., vol. 7, pp. 1–9, 2018, doi: 10.1242/bio.035279.


20. Zushi, S. Kajiwara, and N. Matsuzoe, “Chlorophyll a fluorescence OJIP transient as a tool to characterize and evaluate response to heat and chilling stress in tomato leaf and fruit,” Sci. Hortic. (Amsterdam)., vol. 148, pp. 39–46, Dec. 2012, doi: 10.1016/j.scienta.2012.09.022.


21. Mehta, S. I. Allakhverdiev, and A. Jajoo, “Characterization of photosystem II heterogeneity in response to high salt stress in wheat leaves (Triticum aestivum),” Photosynth. Res., vol. 105, no. 3, pp. 249–255, Sep. 2010, doi: 10.1007/s11120-010-9588-y.


22. Xia, Y. Li, and D. Zou, “Effects of salinity stress on PSII in Ulva lactuca as probed by chlorophyll fluorescence measurements,” Aquat. Bot., vol. 80, no. 2, pp. 129–137, Oct. 2004, doi: 10.1016/j.aquabot.2004.07.006.


23. M. Kalaji, Govindjee, K. Bosa, J. Kościelniak, and K. Zuk-Gołaszewska, “Effects of salt stress on photosystem II efficiency and CO2 assimilation of two Syrian barley landraces,” Environ. Exp. Bot., vol. 73, no. 1, pp. 64–72, Nov. 2011, doi: 10.1016/j.envexpbot.2010.10.009.


24. A. Bacarin, S. Deuner, F. S. P. da Silva, D. Cassol, and D. M. Silva, “Chlorophyll a fluorescence as indicative of the salt stress on Brassica napus L,” Brazilian J. Plant Physiol., vol. 23, no. 4, pp. 245–253, 2011, doi: 10.1590/S1677-04202011000400001.


25. Koller, V. Holland, and W. Brüggemann, “Effects of drought stress on the evergreen Quercus ilex L., the deciduous Q. robur L. and their hybrid Q. × turneri Willd,” Photosynthetica, vol. 51, no. 4, pp. 574–582, 2013, doi: 10.1007/s11099-013-0058-6.


26. Kocheva, P. Lambrev, G. Georgiev, V. Goltsev, and M. Karabaliev, “Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress,” Bioelectrochemistry, vol. 63, no. 1–2, pp. 121–124, Jun. 2004, doi: 10.1016/j.bioelechem.2003.09.020.


27. J. Strauss, G. H. J. Krüger, R. J. Strasser, and P. D. R. V Heerden, “Ranking of dark chilling tolerance in soybean genotypes probed by the chlorophyll a fluorescence transient O-J-I-P,” Environ. Exp. Bot., vol. 56, no. 2, pp. 147–157, Jun. 2006, doi: 10.1016/j.envexpbot.2005.01.011.


28. H. Chang, K. T. Cheng, P. C. Huang, Y. Y. Lin, L. J. Cheng, and T. S. Cheng, “Oxidative stress in greater duckweed (Spirodela polyrhiza) caused by long-term NaCl exposure,” Acta Physiol. Plant., vol. 34, no. 3, pp. 1165–1176, 2012, doi: 10.1007/s11738-011-0913-7.


29. M. Kalaji et al., “Identification of nutrient deficiency in maize and tomato plants by in vivo chlorophyll a fluorescence measurements.,” Plant Physiol. Biochem., vol. 81, no. April, pp. 16–25, Aug. 2014, doi: 10.1016/j.plaphy.2014.03.029.


30. M. Kalaji, L. Račková, V. Paganová, T. Swoczyna, S. Rusinowski, and K. Sitko, “Can chlorophyll-a fluorescence parameters be used as bio-indicators to distinguish between drought and salinity stress in Tilia cordata Mill?,” Environ. Exp. Bot., vol. 152, no. October 2017, pp. 149–157, 2018, doi: 10.1016/j.envexpbot.2017.11.001.