تاثیر نانوکیتوزان حامل عصاره ازمک بر سطح آنتی اکسیدان ها و پراکسیداسیون لیپیدی بافت کبد موش صحرایی آلوده به استافیلوکوکوس اورئوس
محورهای موضوعی : میکروبیولوژی
آناهیتا سینائی
1
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مرتضی مهاجری امیری
2
,
زهرا کشتمند
3
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1 - 1- گروه زيست شناسي، واحد علوم و تحقیقات، دانشگاه آزاد اسلامي، تهران، ایران
2 - گروه زيست شناسي، واحد علوم و تحقیقات، دانشگاه آزاد اسلامي، تهران، ایران
3 - گروه‌زیست‌شناسی،دانشکده‌علوم‌پایه،واحد‌تهران‌مرکزی،دانشگاه‌آزاد‌اسلامی،تهران،ایران.
کلید واژه: نانوکیتوزان, گیاه ازمک, آنتی اکسیدان, پراکسیداسیون لیپید, کبد, استافیلوکوکوس اورئوس, موش صحرایی,
چکیده مقاله :
زیست سازگاری، زیست تخریب پذیری، خاصیت ضد باکتریایی و غیر سمی بودن نانوکیتوزان و استفاده از ترکیبات گیاهی با خاصیت به عنوان مواد ضد باکتریایی امروزه مورد توجه قرار گرفته است. هدف از این پژوهش بررسی اثر نانوکیتوزان حامل عصاره ازمک بر تغییرات سطح آنتی اکسیدانها و پراکسیداسیون لیپید بافت کبد آلوده به باکتری استافیلوکوکوس اورئوس در موشهای صحرایی نر نژاد ویستار میباشد. در این مطالعه تجربی 28 موش صحرایی نر نژاد ویستار، در چهار گروه شامل گروه کنترل ،آلوده به استافیلوکوکوس اورئوس CFU / ml)108 )، دریافت کننده نانوکیتوزان(ml 1) و مدل آلوده + دریافت کننده نانوکیتوزان حامل گیاه ازمک (mg/kg 200) تقسیم بندی شدند. القا آلودگی به روش تزریق درون صفاقی، تک دوز و دریافت نانوکیتوزان حامل عصاره گیاه ازمک با روش گاواژ به مدت 30 روز انجام شد. بعد از دوره تیمار و تشریح حیوانات، بافت کبد جهت بررسی سطح فعالیت آنتیاکسیدان تام، سوپراکسیددیسموتاز، گلوتاتیون پراکسیداز، کاتالاز و مالون دی آلدهید گروه های مختلف استخراج شد. آنالیز دادهها در گروههای مختلف با نرم افزار SPSS و آزمون آماری واریانس یکطرفه انجام و 0.05>P معنی‌دار در نظر گرفته شد. نتایج حاصل، تغییر معنادار سطح آنتی اکسیدان تام، سوپراکسیددیسموتاز، گلوتاتیون پراکسیداز، کاتالاز و مالوندیآلدهیدگروههای آلوده را در مقایسه با گروه کنترل نشان داد (0.05>P). در حالیکه کاهش سطح مالون دی آلدهید، افزایش سطح آنتی اکسیدان تام، سوپراکسیددیسموتاز، گلوتاتیون پراکسیداز و کاتالاز در گروههای تیمار در مقایسه با گروه آلوده به صورت معنادار نشان داده شد (0.05>P). بر اساس نتایج به دست آمده احتمالا به دلیل ترکیبات و اثرات آنتتی اکسیدانی، نانوکیتوزان حامل عصاره گیاه ازمک اثر تعدیلکنندگی بر سطح آنتیاکسیدانها و پراکسیداسیون لیپید بافت کبد موشهای صحرایی آلوده به باکتری را نشان داد. بنابراین احتمالا بتواند به عنوان یک راهکار امیدوارکننده درمانی استفاده شود.
The biocompatibility, biodegradability, antibacterial and non-toxic properties of nanochitosan and the use of plant compounds with antibacterial properties have been considered today. The aim of this study was to investigate the effect of nanochitosan carrying Lepidium draba extract on changes in the level of antioxidants and lipid peroxidation of liver tissue infected with Staphylococcus aureus in male Wistar rats. In this experimental study, 28 male Wistar rats were divided into four groups including control group, infected with Staphylococcus aureus (108 CFU/ml), receiving nanochitosan (1ml), and infected model + receiving nanochitosan carrying Lepidium draba extract (200 mg/kg). Infection was induced by intraperitoneal injection, single dose, and receiving nanochitosan carrying the Lepidium draba extract by gavage for 30 days.After the treatment period and animal dissection, liver tissue was extracted to examine the activity levels of total antioxidant, superoxide dismutase, glutathione peroxidase, catalase, and malondialdehyde of different groups. Data analysis in different groups was performed with SPSS software and one-way variance statistical test, and p<0.05 was considered significant. The results showed a significant change in the levels of total antioxidant, superoxide dismutase, glutathione peroxidase, catalase and malondialdehyde in the infected groups compared to the control group (P<0.05). While a decrease in the level of malondialdehyde, an increase in the level of total antioxidant, superoxide dismutase, glutathione peroxidase and catalase in the treatment groups compared to the infected group was shown to be significant (P<0.05). Based on the results obtained, probably due to its antioxidant compounds and effects, nanochitosan carrying Lepidium draba extract showed a modulating effect on the level of antioxidants and lipid peroxidation in the liver tissue of bacteria-infected rats. Therefore, it can probably be used as a promising therapeutic strategy.
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