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کد مقاله : VARZESH-2101-1005 (R2) بازدید : 65 صفحه: 26 - 36

10.30495/varzesh.2021.1919423.1005

نوع مقاله: پژوهشی

اثر هشت هفته تمرین استقامتی همراه با مصرف عصاره پسته وحشی (بنه) بر بیان ژن گرلین بافت روده موش های صحرایی

محورهای موضوعی : دسترسی آزاد

صالح رحمتی احمدآباد 1 (گروه تربیت‌بدنی، واحد پردیس، دانشگاه آزاد اسلامی، پردیس، ایران)

تاریخ دریافت : 1399/10/12 تاریخ پذیرش : 1400/01/12 تاریخ انتشار : 1399/12/01

کلید واژه: روده, گرلین, تمرین هوازی, پسته وحشی (بنه),

چکیده مقاله :

مقدمه و هدف: بر هم خوردن تعادل انرژی موجب بروز بیماری های مختلفی مانند چاقی، دیابت و غیره می شود. پپتیدهای زیادی در تنظیم تعادل انرژی در بدن نقش دارند که گرلین یکی از مهم ترین آن ها است. این مطالعه اثر یک دوره تمرین استقامتی و عصاره پسته وحشی (بنه) (به‌عنوان گیاهی غنی از اسیدهای چرب)، بر بیان ژن گرلین بافت روده موش‌های صحرایی ماده را بررسی می نماید. مواد و روش ها: در این مطالعه تجربی 20 سر موش صحرایی ماده بالغ نژاد ویستار انتخاب و به‌طور تصادفی به چهار گروه 1) کنترل سالین، 2) تمرین سالین، 3) کنترل بنه و 4) تمرین + بنه تقسیم شدند. گروه‌های 2 و 4 به مدت هشت هفته (پنج روز در هفته، هر جلسه به مدت 60 دقیقه و سرعت 25 متر بر دقیقه با شیب صفر) روی نوارگردان مخصوص جوندگان به تمرین پرداختند در حالی که گروه های 1 و 3 تمرین نداشتند. گروه‌های 3 و 4 عصاره پسته وحشی و گروه‌های 1 و 3 محلول سالین را قبل از تمرین به صورت گاواژ دریافت می‌کردند (یک میلی گرم به ازای هر گرم وزن بدن). 72 ساعت پس از آخرین جلسه ی تمرینی در وضعیت ناشتایی، موش های صحرایی بیهوش شده و بافت‌برداری انجام شد. داده‌ها با استفاده از آزمون آنالیز واریانس دو‌طرفه آنالیز شدند و 05/0> p معنی‌دار در نظر گرفته شد. یافته ها: هشت هفته تمرین هوازی منجر به کاهش بیان ژن گرلین در بافت روده موش های صحرایی شد (001/0> p ) اما بیان آن در پاسخ به استفاده از پسته وحشی افزایش یافت (001/0> p ). بحث و نتیجه گیری: استفاده از رژیم غذایی پرچرب (عصاره بنه) باعث افزایش بیان ژن گرلین بافت روده شد. انجام هشت هفته تمرین هوازی این افزایش را تعدیل نمود.

چکیده انگلیسی:

Introduction: Impaired energy balance causes various diseases such as obesity, diabetes, etc. Many peptides are involved in regulating energy balance in the body, of which ghrelin is one of the most important. This study investigates the effect of endurance training and Pistacia Atlantica extract (Baneh) (as a plant rich in fatty acids) on ghrelin gene expression in the intestinal tissue of female rats. Materials and methods: In this experimental study, 20 adult female Wistar rats were selected and randomly divided into four groups: 1) saline control, 2) saline training, 3) Baneh control, and 4) Baneh + training. Groups 2 and 4 trained on a rodent treadmill for eight weeks (five days a week, each session for 60 minutes at a speed of 25 m / min with a zero slope) while groups 1 and 3 did not. Groups 3 and 4 received Pistacia Atlantica extract and groups 1 and 3 received saline solution before exercise as a gavage (1 mg per gram of body weight). Rats were anesthetized and biopsied 72 hours after the last training session in the fasting state. Data were analyzed using a two-way analysis of variance and p < 0.05 was considered significant. Findings: Eight weeks of aerobic training decreased ghrelin gene expression in rats intestinal tissue (p < 0.001) but its expression increased in response to Pistacia Atlantica use (p < 0.001). Conclusion: The use of a high-fat diet (Baneh extract) increased the expression of the ghrelin gene in intestinal tissue. Eight weeks of aerobic training moderated this increase.

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