اثر هشت هفته تمرین هوازی بعد از تزریق سلول بنیادی مشتق از مغز استخوان بر مقادیر TNF-α و NF-KB بافت کبد موش های مدل کبد چرب غیر الکلی
محورهای موضوعی : علوم ورزش
حامد نامدار
1
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سید عبداله هاشم ورزی
2
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عبدالرضا جعفری
3
1 - دانشجوی دکتری فیزیولوژی ورزشی،گروه فیزیولوژی ورزش، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران
2 - استادیار گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران
3 - 3. استادیار گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران
کلید واژه: تمرین, سلول بنیادی, التهاب, کبد چرب غیر الکلی,
چکیده مقاله :
کبد چرب غیرالکلی از رایجترین بیماریهای کبدی قرن حاضر است که با تغییر سایتوکاینهای التهابی همراه است. هدف پژوهش حاضر، بررسی اثر تمرین هوازی بعد از تزریق سلول بنیادی مشتق از مغز استخوان بر مقادیر TNF-α و NF-KB بافت کبد در موشهای مدل کبد چرب بود. 36 سر موش نژاد ويستار، ابتدا به دو گروه اصلی کنترل (6 سر) و بیمار کبد چرب (30 سر) تقسیم شدند. گروه کنترل 6 هفته غذای استاندارد جوندگان و گروههای بیمار، غذای پرچرب برای القای بیماری کبد چرب استفاده کردند. در پایان هفته ششم، جهت تایید ایجاد بیماری کبد چرب، به طور تصادفی از دم 3 سر موش خونگیری و آنزیمهای کبد اندازهگیری شد. سپس حیوانات گروه بیمار به طور تصادفی به 5 گروه سالین، کبد چرب، تمرین، سلول و تمرین + سلول تقسیم شدند. به گروههای دریافت کننده سلول، حدود 105 سلول در 2 میکرولیتر محیط، برای هر موش از طریق سیاهرگ دمی تزریق گردید. برنامه تمرین شامل 8 هفته دویدن روی تردمیل به صورت فزاینده بود. مقادیر TNF-α و NF-KB در گروههای تحت درمان کاهش یافت اما این کاهش تنها در گروههای تمرین و تمرین + سلول نسبت به گروه کبد چرب معنیدار بود. تمرین هوازی فزاینده بعد از تزریق سلول بنیادی مشتق از مغز استخوان سبب کاهش شاخصهای التهابی TNF-α و NF-KB در موشهای مبتلا به کبد چرب غیرالکلی گردید. لذا به نظر میرسد این روش بتواند به عنوان یک راهکار درمانی سبب بهبود وضعیت کبدی شده و مورد توجه قرار گیرد.
Non-alcoholic fatty liver disease is one of the most common liver diseases of this century, which is associated with changes in inflammatory cytokines. 36 adult male Wistar rats were divided into two main groups: control (6 rats) and patients with fatty liver (30 rats). The control group used standard food for 6 weeks, but the animals in the diseased groups used high-fat food to induce fatty liver disease. At the end of the sixth week, to confirm the development of fatty liver disease, blood was randomly drawn from the tails of 3 rats and liver enzymes were measured. Then the animals of the patient groups were randomly divided into 5 groups: Saline, Fatty liver, Exercise, Cell, Exercise + Cell. About 105 cells in 2 microliters of environment were injected into the cell receiving groups for each rat through the tail vein. The exercise program consisted of 8 weeks of treadmill running, incrementaly. TNF-α and NF-KB decreased, but these changes were significant only in the groups of exercise and exercise + cells compared to the fatty liver group. Incremental aerobic training after the injection of bone marrow-derived stem cells decreased the inflammatory index of TNF-α and NF-KB in rats with Non-alcoholic fatty liver. Therefore, it seems that this method can be considered as a therapeutic solution and improve liver condition
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