مقایسه مواد زیست فعال و ساختمان تشریحی شاتره ایرانی (Fumaria vaillantii Loise) و شاتره بی‌کاسبرگ (F. asepala Boiss)
محورهای موضوعی :
گیاهی
منیر محسنی
1
,
عذرا عطایی عظیمی
2
,
بابک دلنواز هاشملویان
3
1 - دانشجوی دکتری، گروه زیست‌شناسی، دانشکده کشاورزی، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران.
2 - استاد، گروه زیست‌شناسی، دانشکده کشاورزی، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران
3 - دانشیار، گروه زیست‌شناسی، دانشکده کشاورزی، واحد ساوه، دانشگاه آزاد اسلامی، ساوه، ایران.
تاریخ دریافت : 1400/04/16
تاریخ پذیرش : 1400/06/30
تاریخ انتشار : 1401/01/01
کلید واژه:
ساوه,
پراکسیداز,
شاتره ایرانی,
شاتره بی‌کاسبرگ,
کروماتوگرافی,
فنل,
آلکالوئید,
لیپید,
چکیده مقاله :
هدف: شاتره ایرانی (Fumaria vaillantii) و شاتره بی‌کاسبرگ (F. asepala) دو گیاه علفی یکساله هستند که در اجتماعات گیاهی، جدا یا کنار هم می‌رویند. هدف این پژوهش مقایسه ترکیبات بیوشیمی و ساختمان تشریحی این دو گونه در جهت تعیین نزدیکی خواص دارویی آن‌ها بود.
مواد و روش‌ها: گونه گیاهی مورد مطالعه در اردیبهشت 1398، از بخش مرکزی ساوه جمع‌آوری شدند. برای مقایسه ارزش دارویی برخی از ویژگی‌های دو شاتره شامل مواد مؤثره و ساختمان تشریحی برگ، ساقه، ریشه و میوه با هم مقایسه گردید. میزان لیپید، پروتئین، مواد آلی، قند، فنل، آلکالوئید، انواع کلروفیل، مواد معدنی و فعالیت پراکسیداز با روش‌های کالریمتری، اسپکتروفتومتری و کروماتوگرافی لایه نازک، شناسایی و اندازه‌گیری شدند. ساختمان تشریحی با تهیه برش‌های عرضی، رنگ‌آمیز با آبی متیلن و کارمن زاجی و مطالعه با میکروسکوپ نوری انجام گرفت.
یافته‌ها: اختلاف محتوای لیپید، پروتئین، کلروفیل a و مواد معدنی ساقه، فنل ریشه، آلکالوئید میوه، قند و کلروفیل b، دو گونه معنی‌دار بوده، ولی اختلاف ترکیبات اندام‌های دیگر معنی‌دار نبود. فعالیت پراکسیداز و تعداد ترکیبات فنلی اندام‌های دو گونه اختلاف معنی‌داری داشتند. اسیدهای چرب تقریباً در همه اندام‌ها به جز ساقه شاتره بی‌کاسبرگ مشابه بود. ساختمان تشریحی دو شاتره نیز کاملاً مشابه بود.
نتیجه‌گیری: یافته‌های این پژوهش نشان داد که شاتره ایرانی و بی‌کاسبرگ سرشار از مواد مؤثره و ساختمان تشریحی تقریباً مشابه هستند. این نتیجه نشان‌دهنده یکسان بودن ارزش دارویی آن‌ها است.
چکیده انگلیسی:
Objective: Persian sedge (Fumaria vaillantii) and sepalless sedge (F. asepala) are two annual herbaceous plants that grow separately or together in plant communities. The aim of this research was to compare the biochemical composition and anatomical structure of these two species in order to determine the proximity of their medicinal properties.
Materials and methods: The studied plant species were collected from the central part of Saveh in May 2018. In order to compare the medicinal value, some characteristics of two Fumaria including effective substances and the anatomical structure of leaves, stems, roots and fruits were compared. The amount of lipid, protein, organic matter, sugar, phenol, alkaloid, types of chlorophyll, minerals and peroxidase activity were identified and measured by calorimetry, spectrophotometry and thin layer chromatography methods. Anatomical structure was made by preparing transversal sections, stained with methylene blue and Carmen Zaji and studied with a light microscope.
Findings: The differences in the content of lipid, protein, chlorophyll a and stem minerals, root phenol, fruit alkaloid, sugar and chlorophyll b were significant in two species, but the differences in the composition of other organs were not significant. The activity of peroxidase and the number of phenolic compounds in the organs of the two species were significantly different. Fatty acids were almost similar in all organs except the stem of the leafless shoot. The anatomical structure of the two Fumaria was also completely similar.
Conclusion: The findings of this research showed that Iranian Shatre and Bekasbarg are rich in effective substances and the anatomical structure is almost similar. This result shows that their medicinal value is the same.
منابع و مأخذ:
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Arnon AN. Method of extraction in the plants. Agronomy Journal. 1976; 23: 11-15.
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Bradford MM. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye Binding. Biochem. 1976; 72: 248-254.
Deng Y, Shao Q & Li C. Differential responses of double petal and multi petal jasmine to shading: II. Morphology, anatomy and physiology. Scientia Horticult. 2012; 144: 19-28.
Dixon RA. Plant cell and tissue culture. 1987; Oxford, p. 453.
Dreywood R. Qualitative test for carbohydrate material. J Industrial Eng chem. 1946; 18: 499-506.
Ebrahimzadeh Araii F, Keshavarzi M, Sheidaii M & Ghadam P. Fruit and seed morphology of the Fumaria species of Iran. J. Bot. 2011; 35: 167-173.
Folch J, Lees, M & Hsoane G. A simple method for the isolation and purification of total lipids from animal tissues. Bio.Chem. 1957; 226: 497-509.
Gazizov FY & Glushenkova AI. Phospholipids of the seed Fumaria vaillantii. Chem. Natu.Comp. 1997; 33: 627-629.
Habibi Tirtash F, Keshavarzi M & Fazeli F. Antioxidant components of Fumaria species. J.Biomed.Bio.Engin. 2011, 5(2): 57-60.
Haji Abass F, Irian S, Amanzadeh A, Heidarnejad F, Gudarzi H & Salimi M. Anti-proliferative activity of Fumaria vaillantii extracts on different cancer cell lines. Pharma. Sci. 2016; 11(2): 152-159.
Hu C. Biosynthesis and localization of peanut peroxidase. Physiol. 1989; 135(4): 391-397.
Jozwiak GW & Waksmundzka- Hajnos M. Preparative-layer chromatography of an extract of alkaloids from Fumaria officinalis. Acta Chromatographica. 2007; 18: 207-218.
Kurepin LV. Phenotype plasticity of sun and shade ecotypes of Stellria longis in response to light quality signaling. Environ. Exp. Bot. 2012; 84: 25-32.
Liden M. Notes on Cordalis and Fumaria in Iran. Iranian J. Bot. 2000; 8(2): 303-308.
Mehrabian AR, Azizian D, Zarre SH & Podlech D. Petiole Anatomy in Astragalus Sect. Incani (Fabaceae) in Iran. Iranian J. Bot. 2007; 13: 138-145.
Mirzabaeva MA, Umarov U & Shukurova MR. The oil of Fumaria vaillantii. Khimiya Prirodnykh Soedinenii.1971; 6: 826-827.
Mitsui T & Ikeda S. Nas- benzidine reaction of blood cells. Fol Anat Jap. 1951; 23(4-5): 331-336.
Modi K, Amin A & Shah M. A pharmacognostical study on Fumaria parviflora Natural Remedies. 2016; 16: 1-6.
Paltinean R, Crisan G, Balica G & Ielciu I. Histo-anatomical research on vegetative organs of five Romanian Fumaria Farmacia. 2015; 63(2): 262-266.
Pandy VB, Ray AB & Dasgupta B. Quaternary alkaloids of Fumaria indica, Phytochemistry. 1976; 15(4): 545-546.
Ravikanth K, Kanaujia A, Takur D, Shurma A & Gautam B. Nutritional constituents of the plants Fumaria indica and Caesalpinia bonducella. IJAPBC. 2014; 3(3): 698-702.
Razavi R & Esmaeilzadeh-Kenary R. Antioxidant evaluation of Fumaria parviflora. Journal of food measurement and characterization 2021; 15: 2448-57.
R-Taafar KM & El-Zahaby SA. Niosomes of active Fumaria officinalis Chinese Medicine. 2020; 15: 1-40.
Snafi AEA. Constituent and pharmacology of Fumaria officinalis: A review. Journal of Pharmacy. 2020; 10(1): 17-25.
Sharma UR, Surendra V, Roopakarki NRR & Tigary P. Heptoprtective activity of Fumaria officianalis against CCl4-induced liver damage inrat. Pharmacologia. 2012; 3(1): 9-14.
Si CC, Dai ZC & Lin Y. Local adaptation and phenotypic plasticity both occurred in Wedelia trilobata invasion across a tropical island. Invasions. 2014; 16: 2323-2337.
Vrancheva RZ, Ivanov IG, Aneva IY, Dincheva IN, Badjakov IK & Parlov AI. Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria. Zeitschrift für Naturforschung C. 2016; 71(1-2): 9-14.
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Abrahamsoon, SL, Speiser, DM & Owa A gel electrophoresis assay for photochelations. Anal. Biochem. 1992; 20: 239-243.
Arnon AN. Method of extraction in the plants. Agronomy Journal. 1976; 23: 11-15.
Bamoniri A, Behpour M & Khayat Kashani M. Quantification of total phenolics and tannins of pomegranate extraction. J Optoel Biomed Mate. 2010; 2(1): 25-31.
Bradford MM. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye Binding. Biochem. 1976; 72: 248-254.
Deng Y, Shao Q & Li C. Differential responses of double petal and multi petal jasmine to shading: II. Morphology, anatomy and physiology. Scientia Horticult. 2012; 144: 19-28.
Dixon RA. Plant cell and tissue culture. 1987; Oxford, p. 453.
Dreywood R. Qualitative test for carbohydrate material. J Industrial Eng chem. 1946; 18: 499-506.
Ebrahimzadeh Araii F, Keshavarzi M, Sheidaii M & Ghadam P. Fruit and seed morphology of the Fumaria species of Iran. J. Bot. 2011; 35: 167-173.
Folch J, Lees, M & Hsoane G. A simple method for the isolation and purification of total lipids from animal tissues. Bio.Chem. 1957; 226: 497-509.
Gazizov FY & Glushenkova AI. Phospholipids of the seed Fumaria vaillantii. Chem. Natu.Comp. 1997; 33: 627-629.
Habibi Tirtash F, Keshavarzi M & Fazeli F. Antioxidant components of Fumaria species. J.Biomed.Bio.Engin. 2011, 5(2): 57-60.
Haji Abass F, Irian S, Amanzadeh A, Heidarnejad F, Gudarzi H & Salimi M. Anti-proliferative activity of Fumaria vaillantii extracts on different cancer cell lines. Pharma. Sci. 2016; 11(2): 152-159.
Hu C. Biosynthesis and localization of peanut peroxidase. Physiol. 1989; 135(4): 391-397.
Jozwiak GW & Waksmundzka- Hajnos M. Preparative-layer chromatography of an extract of alkaloids from Fumaria officinalis. Acta Chromatographica. 2007; 18: 207-218.
Kurepin LV. Phenotype plasticity of sun and shade ecotypes of Stellria longis in response to light quality signaling. Environ. Exp. Bot. 2012; 84: 25-32.
Liden M. Notes on Cordalis and Fumaria in Iran. Iranian J. Bot. 2000; 8(2): 303-308.
Mehrabian AR, Azizian D, Zarre SH & Podlech D. Petiole Anatomy in Astragalus Sect. Incani (Fabaceae) in Iran. Iranian J. Bot. 2007; 13: 138-145.
Mirzabaeva MA, Umarov U & Shukurova MR. The oil of Fumaria vaillantii. Khimiya Prirodnykh Soedinenii.1971; 6: 826-827.
Mitsui T & Ikeda S. Nas- benzidine reaction of blood cells. Fol Anat Jap. 1951; 23(4-5): 331-336.
Modi K, Amin A & Shah M. A pharmacognostical study on Fumaria parviflora Natural Remedies. 2016; 16: 1-6.
Paltinean R, Crisan G, Balica G & Ielciu I. Histo-anatomical research on vegetative organs of five Romanian Fumaria Farmacia. 2015; 63(2): 262-266.
Pandy VB, Ray AB & Dasgupta B. Quaternary alkaloids of Fumaria indica, Phytochemistry. 1976; 15(4): 545-546.
Ravikanth K, Kanaujia A, Takur D, Shurma A & Gautam B. Nutritional constituents of the plants Fumaria indica and Caesalpinia bonducella. IJAPBC. 2014; 3(3): 698-702.
Razavi R & Esmaeilzadeh-Kenary R. Antioxidant evaluation of Fumaria parviflora. Journal of food measurement and characterization 2021; 15: 2448-57.
R-Taafar KM & El-Zahaby SA. Niosomes of active Fumaria officinalis Chinese Medicine. 2020; 15: 1-40.
Snafi AEA. Constituent and pharmacology of Fumaria officinalis: A review. Journal of Pharmacy. 2020; 10(1): 17-25.
Sharma UR, Surendra V, Roopakarki NRR & Tigary P. Heptoprtective activity of Fumaria officianalis against CCl4-induced liver damage inrat. Pharmacologia. 2012; 3(1): 9-14.
Si CC, Dai ZC & Lin Y. Local adaptation and phenotypic plasticity both occurred in Wedelia trilobata invasion across a tropical island. Invasions. 2014; 16: 2323-2337.
Vrancheva RZ, Ivanov IG, Aneva IY, Dincheva IN, Badjakov IK & Parlov AI. Alkaloid profiles and acetylcholinesterase inhibitory activities of Fumaria species from Bulgaria. Zeitschrift für Naturforschung C. 2016; 71(1-2): 9-14.
