بررسی تاثیر نانو ذرات خرفهL. Portulaca oleracea و عصاره باکتری پروبیوتیکLactobacillus plantarum بر روی رده سلولی CacoII بر روی بیان ژن های JAKI و JAKII
محورهای موضوعی : زیست شناسی
1 - گروه زیست‌شناسی، واحد قم، دانشگاه آزاد اسلامی، قم، ایران
کلید واژه: پروبيوتيک, Lactobacillus plantarum, سرطان كولون, Portulaca oleracea, CacoII , JAKI, JAKII ,
چکیده مقاله :
چکیده: نانو ذرات خرفه Portulaca oleracea و باکتریهای پروبيوتيك که ميكروارگانيسمهای زنده و مفيد هستند با تأثير بر آنزيمهای گوارشی حيوانات و انسانها، مهار عوامل سرطانزا را در داخل بدن و شرايط آزمايشگاهی و تركيبات القا كننده سرطان و تومورها در حيوانات آزمايشگاهی نقش مؤثری در جهت مقابله با سرطان ايفا میكنند. هدف مطالعه حاضر بررسی تاثیر نانو ذرات خرفه Portulaca oleracea و عصاره باکتری پروبیوتیکLactobacillus plantarum روی رده سلولی CacoII بر روی بیان ژن های JAKI و JAKII میباشد.
مواد و روشها : در این مطالعه که به صورت تجربی انجام شده است از كشت باكتریLactobacillus plantarum، مایع رویی جدا شد.سپس اثرات سمیت سلولی مایع رویی جدا شده از باکتری و نانوذرات خرفه Portulaca oleracea همچنین باکتریها بر روی رده سلولی سرطان كولون CacoII در مدت زمان 24،48و72ساعت با استفاده از روشMTT بررسی شد.همچنین بیان ژنهایJAKII و JAKII با استفاده از روش Real Time PCR مورد بررسی قرار گرفت.
نتایج:. مایع رویی که حاوی باکتری Lactobacillus plantarum باعث کاهش تکثیر سلولی میشوند. نتايج به دست آمده نشان داد كه درصد مهاري محلول رویی (سوپرناتانت) Lactobacillus plantarum روي سلولهاي سرطانی CacoII ، ژنهای JAKI در طول 48 و ۷۲ ساعت افزایش بیان معناداری داشته است. ژنهای JAKII در طول 48 و ۷۲ ساعت کاهش بیان معناداری داشته است.
نتیجهگیری: ميتوان از باكتري Lactobacillus plantarum و نانوذرات خرفه Portulaca oleracea ، جهت ايجاد يك راهكار نوين درماني با تأثير بالا، عوارض جانبي پايين، بيخطر از نظر بيولوژيكي و هزينه كمتر براي درمان و همچنين پيشگيري از سرطان كولون بهره برد.
Introduction: Portulaca oleracea nanoparticles and probiotic bacteria, which are useful microorganisms, which, by affecting the digestive enzymes of animals and humans, inhibit carcinogens in the body and laboratory conditions and compounds that induce cancer and tumors in laboratory animals, play an effective role in They perform to fight cancer. The purpose of this study is to investigate the effect of Portulaca oleracea purslane nanoparticles and Lactobacillus plantarum probiotic bacteria extract on CacoII cell line on the expression of JAKI and JAKII genes.
Materials and methods: In this study, which was conducted experimentally, the supernatant was isolated from the culture of Lactobacillus plantarum. Then, the effects of the cytotoxicity of the supernatant isolated from the bacteria and Portulaca oleracea purslane nanoparticles as well as the bacteria on the CacoII colon cancer cell line during The time of 24, 48 and 72 hours was checked using MTT method. Also, the expression of JAKII and JAKII genes was checked using Real Time PCR method.
Results: Supernatant containing Lactobacillus plantarum bacteria reduces cell proliferation. The obtained results showed that the inhibitory percentage of Lactobacillus plantarum supernatant on CacoII cancer cells, JAKI genes had a significant increase in expression during 48 and 72 hours. JAKII genes had a significant decreased in expression during 48 and 72 hours
Conclusion: Lactobacillus plantarum bacteria and Portulaca oleracea purslane nanoparticles can be used to create a new therapeutic solution with high effect, low side effects, biologically safe and less expensive for the treatment and prevention of colon cancer
1-Liu, L., et al., Fatty acids and β-carotene in Australian purslane (Portulaca oleracea) varieties. Journal of chromatography A, 2000. 893(1): p. 207-213
. 2-Gatreh-Samani, K., et al., Purslane (Portulaca oleracea) effects on serum paraoxanase-1 activity. Journal of Shahrekord Uuniversity of Medical Sciences, 2011. 13.
. 3-Uddin, M., et al., Purslane weed (Portulaca oleracea): a prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes. The Scientific World Journal, 2014.
. 4-Movahedian Ataar, A., et al., Antioxidant Effect of Ziziphus vulgaris, Portulaca oleracea, Berberis integerima and gundelia tournefortti on lipid peroxidation, Hb glycosylation and Red Blood Cell Hemolysis. Journal of Medicinal Plants, 2011. 10(40): p. 80-88
. 5-Valko, M., et al., Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chemico-biological interactions, 2006. 160(1): p. 1-40
. 6-Jiang, M., et al., JAKI: A key protein of cross‐talk signal way in “PANoptosis” in cancer. International journal of cancer, 2021. 149(7): p. 1408-1420
. 7-Li, P., et al., Cytochrome c and dATP-dependent formation of Apaf-1/JAKII complex initiates an apoptotic protease cascade. cell, 1997. 91(4): p. 479-489
. 8-Mesbah, N.M., W.B. Whitman, and M. Mesbah, Determination of the G+ C content of prokaryotes, in Methods in microbiology. 2011, Elsevier. p. 299-324
. 9-Segeritz, C.-P. and L. Vallier, Cell culture: Growing cells as model systems in vitro, in Basic science methods for clinical researchers. 2017, Elsevier. p. 151-172
10-Joshi, C.J., et al., What are housekeeping genes? PLoS computational biology, 2022. 18(7): p. e1010295
. 11-Jin, H., et al., Portulaca oleracea extract can inhibit nodule formation of colon cancer stem cells by regulating gene expression of the Notch signal transduction pathway. Tumor Biology, 2017. 39(7): p. 1010428317708699
. 12-Baricault, L., et al., Use of HT-29, a cultured human colon cancer cell line, to study the effect of fermented milks on colon cancer cell growth and differentiation. Carcinogenesis, 1995. 16(2): p. 245-252
. 13-Biffi, A., et al., Antiproliferative effect of fermented milk on the growth of a human breast cancer cell line. 1997
. 14-Pretlow, T.P., et al., Aberrant crypts: putative preneoplastic foci in human colonic mucosa. Cancer Research, 1991. 51(5): p. 1564-1567
. 15-Altonsy, M.O., S.C. Andrews, and K.M. Tuohy, Differential induction of apoptosis in human colonic carcinoma cells (CacoII) by Atopobium, and commensal, probiotic and enteropathogenic bacteria: mediation by the mitochondrial pathway. International journal of food microbiology, 2010. 137(2-3): p. 190-203
. 16-Ma, E.L., et al., The anticancer effect of probiotic Bacillus polyfermenticus on human colon cancer cells is mediated through ErbB2 and ErbB3 inhibition. International journal of cancer, 2010. 127(4): p. 780-790
. 17-El-Nezami, H., et al., Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media. Journal of food protection, 1998. 61(4): p. 466-468
. 18-Saarela, M., et al., Probiotic bacteria: safety, functional and technological properties. Journal of biotechnology, 2000. 84(3): p. 197-215
. 19-Zheng, G.-Y., et al., Portulacerebroside A induces apoptosis via activation of the mitochondrial death pathway in human liver cancer HCCLM3 cells. Phytochemistry Letters, 2014. 7: p. 77-84
. 20-Lee, D.K., et al., Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines. BMC cancer, 2008. 8(1): p. 1-8
. 21-Köhler, C., S. Orrenius, and B. Zhivotovsky, Evaluation of caspase activity in apoptotic cells. Journal of immunological methods, 2002. 265(1-2): p. 97-110
. 22-Urruticoechea, A., et al., Recent advances in cancer therapy: an overview. Current pharmaceutical design, 2010. 16(1): p. 3-10
. 23-Wollowski, I., G. Rechkemmer, and B.L. Pool-Zobel, Protective role of probiotics and prebiotics in colon cancer. The American journal of clinical nutrition, 2001. 73(2): p. 451s-455s
. 24-Abrahamse, S., B. Pool-Zobel, and G. Rechkemmer, Potential of short chain fatty acids to modulate the induction of DNA damage and changes in the intracellular calcium concentration by oxidative stress in isolated rat distal colon cells. Carcinogenesis, 1999. 20(4): p. 629-634
. 25-Brady, L.J., D.D. Gallaher, and F.F. Busta, The role of probiotic cultures in the prevention of colon cancer. The Journal of nutrition, 2000. 130(2): p. 410S-414S
. 26-Horn, R.C. and V.M.F. Vargas, Antimutagenic activity of extracts of natural substances in the Salmonella/microsome assay. Mutagenesis, 2003. 18(2): p. 113-118
. 27-Gahyva, S.M.M. and J.F. Siqueira Junior, Direct genotoxicity and mutagenicity of endodontic substances and materials as evaluated by two prokaryotic test systems. Journal of Applied Oral Science, 2005. 13: p. 387-392
. 28-Shah, N., Probiotic bacteria: selective enumeration and survival in dairy foods. Journal of dairy science, 2000. 83(4): p. 894-907
. 29-Hirayama, K. and J. Rafter, The role of probiotic bacteria in cancer prevention. Microbes and infection, 2000. 2(6): p. 681-686
. 30-Rowland, I., P. Grasso, and M. Davies, The methylation of mercuric chloride by human intestinal bacteria. Experientia, 1975. 31(9): p. 1064-1065
. 31-Matsuzaki, T., Immunomodulation by treatment with Lactobacillus casei strain Shirota. International journal of food microbiology, 1998. 41(2): p. 133-140
. 32-Sekine, K., et al., A new morphologically characterized cell wall preparation (whole peptidoglycan) from Bifidobacterium infantis with a higher efficacy on the regression of an established tumor in mice. Cancer Research, 1985. 45(3): p. 1300-1307
. 33-Kich, D.M., et al., Probiotic: effectiveness nutrition in cancer treatment and prevention. Nutricion hospitalaria, 2016. 33(6): p. 1430-1437
. 34-Guandalini, S., E. Cernat, and D. Moscoso, Prebiotics and probiotics in irritable bowel syndrome and inflammatory bowel disease in children. Beneficial microbes, 2015. 6(2): p. 209-217
. 35-Whelan, K., Probiotics and prebiotics in the management of irritable bowel syndrome: a review of recent clinical trials and systematic reviews. Current Opinion in Clinical Nutrition & Metabolic Care, 2011. 14(6): p. 581-587
. 36-Barrons, R. and D. Tassone, Use of Lactobacillus probiotics for bacterial genitourinary infections in women: a review. Clinical therapeutics, 2008. 30(3): p. 453-468