بررسی تاثیر نانو ذرات خرفه .Portulaca oleracea Lو عصاره باکتری پروبیوتیک Lactobacillus plantarumبر روی رده سلولی CacoII بر روی بیان ژن های CASPAS8 و CASPAS 9
محورهای موضوعی : زیست شناسی
1 - دانشگاه آزاد اسلامی واحد قم
کلید واژه: پروبيوتيک, Lactobacillus plantarum, سرطان كولون, Portulaca oleracea, CacoII , CASPAS۹ , CASPAS8 ,
چکیده مقاله :
مقدمه: نانو ذرات خرفه Portulaca oleracea و ضمنا باکتریهای پروبيوتيك که ميكروارگانيسمهای زنده و مفيدی هستند كه با تأثير بر آنزيمهای گوارشی حيوانات و انسانها، مهار عوامل سرطان زا را در داخل بدن و شرايط آزمايشگاهی و تركيبات القا كننده سرطان و تومورها در حيوانات آزمايشگاهی نقش مؤثری در جهت مقابله با سرطان ايفا میكنند. هدف مطالعه حاضر بررسی تاثیر نانو ذرات خرفه Portulaca oleracea و عصاره باکتری پروبیوتیکLactobacillus plantarum روی رده سلولی CacoII بر روی بیان ژن های CASPAS8 و CASPAS 9 میباشد.
مواد و روشها : در این مطالعه که به صورت تجربی انجام شده است از كشت باكتریLactobacillus plantarum، مایع رویی جدا شد.سپس اثرات سمیت سلولی مایع رویی جدا شده از باکتری و نانوذرات خرفه Portulaca oleracea همچنین باکتریها بر روی رده سلولی سرطان كولون CacoII در مدت زمان 24،48و72ساعت با استفاده از روشMTT بررسی شد.همچنین بیان ژنهایCASP8 و CASP9 با استفاده از روش Real Time PCR مورد بررسی قرار گرفت.
نتایج:. مایع رویی که حاوی باکتری Lactobacillus plantarum باعث کاهش تکثیر سلولی میشوند. نتايج به دست آمده نشان داد كه درصد مهاري محلول رویی (سوپرناتانت) Lactobacillus plantarum روي سلولهاي سرطانی CacoII ، ژنهای CASP8وCASP9 در طول 48 ساعت افزایش بیان معناداری داشته است.
نتیجهگیری: ميتوان از باكتري Lactobacillus plantarum و نانوذرات خرفه Portulaca oleracea ، جهت ايجاد يك راهكار نوين درماني با تأثير بالا، عوارض جانبي پايين، بيخطر از نظر بيولوژيكي و هزينه كمتر براي درمان و همچنين پيشگيري از سرطان كولون بهره برد.
Introduction: Portulaca oleracea purslane nanoparticles and probiotic bacteria, which are living and useful microorganisms, which, by affecting the digestive enzymes of animals and humans, inhibit carcinogens in the body and laboratory conditions and compounds that induce cancer and tumors in laboratory animals, play an effective role in They perform to fight cancer. The purpose of this study is to investigate the effect of Portulaca oleracea purslane nanoparticles and Lactobacillus plantarum probiotic bacteria extract on CacoII cell line on the expression of CASPAS8 and CASPAS9 genes.
Materials and methods: In this study, which was conducted experimentally, the supernatant was isolated from the culture of Lactobacillus plantarum. Then, the effects of the cytotoxicity of the supernatant isolated from the bacteria and Portulaca oleracea purslane nanoparticles as well as the bacteria on the CacoII colon cancer cell line during The time of 24, 48 and 72 hours was checked using MTT method. Also, the expression of CASP8 and CASP9 genes was checked using Real Time PCR method.
Results: Supernatant containing Lactobacillus plantarum bacteria reduces cell proliferation. The obtained results showed that the inhibitory percentage of Lactobacillus plantarum supernatant on CacoII cancer cells, CASP8 and CASP9 genes had a significant increase in expression during 48 hours.
Conclusion: Lactobacillus plantarum bacteria and Portulaca oleracea purslane nanoparticles can be used to create a new therapeutic solution with high effect, low side effects, biologically safe and less expensive for the treatment and prevention of colon cancer.
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