تاثیر اسید سالیسیلیک بر شاخص‌هاي جوانه‌زنی گیاهچه گندم (Triticum aestivum L.) تحت تنش شوری
محورهای موضوعی : تکنولوژی بذر
1 - دکتری، اکولوژی گیاهان زراعی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران
2 - استاد، گروه زراعت، دانشکده کشاورزی دانشگاه محقق اردبیلی، اردبیل، ایران
کلید واژه: اسید سالیسیلیک, پرایمینگ, جوانه‌زنی, کلرید سدیم ,
چکیده مقاله :
به‌منظور بررسي تاثیر اسید سالیسیلیک بر شاخص‌هاي جوانه‌زنی در گیاهچه گندم تحت تنش شوری آزمایشی به‌صورت فاكتوريل در قالب طرح آماري كاملا تصادفي با سه تكرار در سال 1403 در دانشگاه محقق اردبیلی اجرا شد. تیمارها شامل چهار سطح شوری (0، 50، 100 و 200 میلی‌مولار) و چهار سطح اسید سالیسیلیک (صفر، 1/0، 5/0 و 1 میلی‌مولار) بود. نتايج نشان داد كه تنش شوري موجب كاهش درصد جوانه‌زنی، سرعت جوانه‌زنی، میانگین جوانه‌زنی روزانه، طول گیاهچه، وزن خشک گیاهچه و شاخص وزنی بنيه گیاهچه و باعث افزایش سرعت جوانه‌زنی روزانه گردید. اما پرایمینگ بذر با اسید سالیسیلیک به‌ویژه اسید سالیسیلیک یک میلی‌مولار این صفات را بهبود بخشید. البته، مقايسه ميـانگين‌هـا نشـان دهنده آن بود كه بين سطوح اسید سالیسیلیک اخـتلاف معنـي‌داري وجود داشت، به نحوي‌كه بـيش‌تـرين مقدار صفات از كاربرد اسید سالیسیلیک یک میلی‌مولار و كـم‌تـرين آن از عـدم كـاربرد اسید سالیسیلیک حاصل گردید. مقدار شاخص طولی بنیه گیاهچه، وزن تر گیاهچه و درصد رطوبت گیاهچه در پرایمینگ با اسید سالیسیلیک یک میلی‌مولار و بدون شوری به ترتیب در حدود 75، 83 و 46 درصد نسبت به شاهد (آب مقطر) و شوری 200 میلی‌مولار بیشتر بودند. همچنین، بیش‌ترین میانگین مدت جوانه‌زنی در شاهد (پرایمینگ با آب مقطر) و شوری 200 میلی‌مولار مشاهد شد. نتایج این پژوهش نشان می‌دهد که در بین تیمارهاي مورد مطالعه، تيمار بذر با اسید سالیسیلیک یک میلی‌مولار مؤثرترین روش براي بهبود جوانه‌زنی بذر گندم محسوب می‌شود و مي‌تواند اثرات منفی تنش شوري را بر برخي صفات در گياهچه كاهش داده و رشد گياهچه را بهبود بخشد.
In order to investigate the effect of salicylic acid on germination indices in wheat seedling under salinity stress and a factorial experiment was conducted based on completely randomized design at University of Mohaghegh Ardabili in 2024 with 3 replications. Treatments were four salinity levels (0, 50, 100 and 200 mM) and four levels of salicylic acid (0, 0.1, 0.5 and 1 mM). The results showed that salinity stress decreased germination percentage, germination rate, mean daily germination, seedling length, seedling dry weight and seedling weight vigor index and increased daily germination speed. But seed priming with salicylic acid, especially the level of 1 mM, improved these traits. The comparison of the means showed that there was a significant difference between the levels of salicylic acid, so that the highest amount of traits was obtained from the use of 1mM salicylic acid and the lowest was obtained from without salicylic acid use. Seedling length vigor index, seedling Fresh weight and seedling moisture percentage in 1mM salicylic acid priming and no salinity were higher about 75, 83 and 46% compared to the control and 200 mM salinity, respectively. Also, the highest mean germination time was observed in control 200 mM salinity. The results of this study showed that among the different treatments, priming of seeds with 1mM salicylic acid may be considered as an effective way to improve seed germination of wheat and can reduce the harmful effects of salinity stress on some traits in wheat seedlings and improve seedling growth.
Aberkane, H., Payne, T., Kishi, M., Smale, M., Amri, A. and Jamora, N. 2020. Transferring diversity of goat grass to farmers’ fields through the development of synthetic hexaploid wheat. Food Secur. 12(4): 1017-1033.
Afzal, I., Basra, S. M. A., Hameed, A. and Farooq, M. 2006. Physiological enhancements for alleviation of salt stress in wheat. Pak. J. Bot. 38: 1649-1659
Akram, W., Hussain, A., Roomi, I. and Muhammad, M. 2023. Effect of different priming agents on germination of purslane (Portulaca oleracea L.) seeds under laboratory conditions. Pak. J. Bot. 55: 1605-1613.
Albaji, Z. and Marashi, S. K. 2024. Effect of seed priming with hydrogen peroxide on germination indices and seedling growth of wheat (Triticum aestivum L.) under salt stress condition. Iran. J. Seed Sci. Technol. 12(4): 57-67.
Amirikia, F., Nabipour, M. and Farzaneh, M. 2023. Effect of hydro and hormone priming with gibberellin on germination, seedlings emergence and some growth characters in two
Alhagi species (Alhagi graecorum Khuzestan ecotype and Alhagi maurorum Esfahan
ecotype) under saline conditions of using seawater of Persian Gulf. Iran. J. Seed Sci. Res. 10: 63-80.
Ansari Ardali, S., Nabipour, M., Roshanfekr, H. A. and Bagheri, M. 2021. Evaluation of quinoa (Chenopodium quinoa Wild.) cultivars in saline conditions using germination indices in controlled environment. Crop Sci. 14: 475-485.
Arbaoui, M., Yahia, N. and Belkhodja, M. 2015. Germination of the tomato (Lycopersicon
esculentum Mill.) in response to salt stress combined with hormones. Int. J. Agron. Agri. Res. 7(3): 14-24.
Ashraf, M., Nisa, Y. Z. N., Ali, M.Y., Shani, A., Naz, M., Azmat, A. and Ashraf, I. 2023. Salicylic acid seed priming improved dry biomass and ionic efficiency of mungbean [Vigna radiata (L.) Wilczek] under salt stress conditions. Pak. J. Bot. 55: 1615-1622.
Bahrasemani, S., Seyedi, A., Fathi, S. H. and Jowkar, M. 2024. The Seed Priming using Putrescine Improves, Germination Indices and Seedlings morphobiochemical Responses of Indigo (Indigofera tinctoria) under Salinity Stress. J. Med. Plants Byprod. 13(1):179-188.
Batool, N., Ilyas, N., Noor, T., Saeed, M., Mazhar, R., Bibi, F. and Shahzad, A., 2014. Evaluation of drought stress effects on germination and seedling growth of Z. mays L. Int. J. Biosci. 5: 203–209.
Bewley, J. D., Bradford, K. J., Hilhorst, H. W. M. and Nonogaki, H. 2013. Seeds: Physiology of development, germination and dormancy (3rd edition). Seed Sci. Res. 23(4): 289.
Bittebcourt, M. L. C., Dais, D. C. F. S., Dias, L. A. S. and Araujo, E. F. 2004. Effect of priming on asparagus seed germination and vigor under water and temperature stress. J. Seed
Sci. Technol. 32: 607-616.
Debez, A., Ben Slimen, I. D., Bousselmi, S., Atia, A., Farhat, N., El Kahoui, S. and Abdelly, C. 2020. Comparative analysis of salt impact on sea barley from semi-arid habitats in Tunisia and cultivated barley with special emphasis on reserve mobilization and stress recovery aptitude. Plant Biosyst.—Int. J. Deal. All Asp. Plant Biol. 154: 544–552.
Ebrahimi, O., Esmaili, M. M., Sabori, H. and Tahmasebi, A. 2013. Effects of salinity and drought stress on germination of two species of (Agropyron elongatum, Agropyron desertrum). Desert Eco. Eng. J. 1: 31-38.
Ellis, R. and Roberts, E. H. 1980. Towards a rational basis for testing seed quality P605-635.
In: P.D. Hebblethwaite (eds). Seed Production. Butterworth’s. London.
Fabian, A., Jager, K. and Barnabas, B. 2008. Effects of drought and combined drought and heat stress on germination ability and seminal root growth of wheat (Triticum aestivum L.) seedlings. Acta Biol. Szeged. 52: 157-159.
Farooq, M., Basra, S. M. A. and Khan, M. B. 2007. Seed priming improves growth of nursery seedlings and yield of transplanted rice. Arch. Agro. Soil Sci. 53(3): 315–326.
Farouk, S. and Al-Huqail, A. A. 2022. Sustainable biochar and/or melatonin improve salinity tolerance in borage plants by modulating osmotic adjustment, antioxidants, and ion homeostasis. Plants. 11(6): 765.
Finch-Savage, W. E. and Bassel, G. W. 2016. Seed vigour and crop establishment: Extending performance beyond adaptation. J. Exp. Bot. 67: 567–591.
Galviz-Fajardo, Y. C., Bortolin, G. S., Deuner, S., Amarante, L. D., Reolon, F. and Moraes, D. M. D. 2020. Seed priming with salicylic acid potentiates water restriction-induced effects in tomato seed germination and early seedling growth. J. Seed Sci. 42. e202042031.
Ghanbari, M., Mokhtassi-Bidgoli, A., Talebi-Siah Saran, P. and Pirani. H. 2019. Effect of deterioration on germinationVand enzymes activity in dry bean (Phaseolus vulgaris L.) under salinity stress condition. Environ. Stress Crop Sci. 12: 585-594.
Ghasemi, N., Omidi, H. and Bostani A. 2021. Morphological properties of Catharanthus roseus L. seedlings affected by priming techniques under natural salinity stress. J. Plant Growth Regul. 40(2):550-557.
Golizadeh, S. K., Mahmoodi, T. M. and Khaliliaqdam, N. 2015. Effect of priming of (KNO3, ZnSO4, distilled water) on rate germination and seedling establishment on cannabis seed (Cannabis sativa L.). Biol. Forum-An Inter J. 7: 190-194.
Habibi G. and Hosseini Nejad O. 2020. Salicylic acid and hydrogen peroxide priming as a means to induce salt stress tolerance of wheat. J. Plant Res. 33(4):827-839.
Hasanuzzaman, M., Raihan, M. R. H., Masud, A. A. C., Rahman, K., Nowroz, F., Rahman, M., Hawrylak-Nowak, B., Rubinowska, K., Molas, J., Woch, W., Matraszek-Gawron, R. and Szczurowska, A. 2019. Selenium-induced improvements in the ornamental value and salt stress resistance of Plectranthus scutellarioides (L.) R. Br. Folia Horti. 31: 213-221.
Hoogenboom, G. and Peterson, C. M. 1987. Shoot growth rate of soybean as affected by drought stress. Agronomy. 79(4): 598-607.
Hussain, S., Khan, F., Hussain, H. A. and Nie, L. 2016. Physiological and biochemical mechanisms of seed priming-induced chilling tolerance of rice cultivars. Front. Plant Sci. 7: 116.
Iqbal, H., Yaning, C., Rehman, H., Waqas, M., Ahmed, Z., Raza, S. T. and Shareef, M. 2020. Improving heat stress tolerance in late planted spring maize by using different exogenous elicitors. Chil. J. Agric. Res. 80: 30–40.
ISTA. 2013. International Rules for Seed Testing. Bassersdorf, Switzerland: The International Seed Testing Association (ISTA).
Izadi, Y., Moosavi, S. A., Gharineh, M. H., 2022. Salinity affects eco-physiological aspects and biochemical compositions in chia (Salvia hispanica L.) during germination and seedling growth. Sci. Horti. 306, 111461.
Jahanbakhsh, S., Parmon, G. H., Azad, H., and Ghatei, A. 2019. Modeling
hydrotime and threshold tolerance to salinity and drought on germinationdifferent species Basil (Ocimum basilicum). Iran. J. Seed Sci. Technol. 7(2): 119-142.
Jayakannan, M., Bose, J., Babourina, O., Rengel, Z. and Shabala S. 2015. Salicylic acid in plant salinity stress signalling and tolerance. Plant Growth Regul. 76(1): 25-40.
Jiang, W. 2020. Natural Rubber and Bio-based Thermoplastic Elastomer. Industrial Appl.
Bio. Envir. 241–254.
Johnson, R. and Puthur, J. T. 2021. Seed Priming as a Cost Effective Technique for Developing Plants with Cross Tolerance to Salinity Stress. Plant Physiol. Biochem. 162: 247–257.
Kaur, H., Kaur, H., Kaur, H. and Srivastava, S. 2022. The beneficial roles of trace
and ultratrace elements in plants. Plant Growth Regul. 98(3): 1–18.
Khan, W. M., Prithiviraj, B. and Smiyh, D. L. 2002. Effect of foliar application of chitin oligosaccharides on photosynthesis of maize and soybean. Photosynthetica. 40: 621-624.
Kibinza, S., Bazin, J., Bailly, C., Farrant, J. M., Corbineau, F. and El-Maarouf-Bouteau, H. 2011. Catalase is a key enzyme in seed recovery from ageing during priming. Plant Sci. 181(3): 309–315.
Kulak, M., Jorrín-Novo, J. V., Romero-Rodriguez, M. C., Yildirim, E. D., Gul, F. and Karaman S. 2021. Seed priming with salicylic acid on plant growth and essential oil composition in basil (Ocimum basilicum L.) plants grown under water stress conditions. Ind. Crops Prod. 161: 113235.
Madady, M., Khomari, S., Javadi, A. and Sofalian, A. 2016. The effect of priming with calcium nitrate and zinc oxide on seed germination and seedling growth of corncockle under salinity stress. J. Plant Pro. Fun. 5(15): 169-179.
Maguire, J. D. 1962. Speed of germination, aid in selection and evaluation for seedling emergence and vigour. Crop Sci. 2:176-177.
Mansouri Gandomani, V., Omidi, H. and Bostani, A. A. 2019. Study on effects of pretreatment nanoparticle silicon dioxide (SiO2) on seed germination and biochemical indicate of soybean (Glycine max L.) cultivars Williams under salinity. Iranian Journal of Seed Science and Research, 6(3): 299-315.
Mazor, L., Perl, M. and Negbi, M. 1984. Changes in some ATP dependent activities in seeds during treatment with polyethylene glycol and during the redlying process. J. Exp. Bot. 35: 1119-1127.
Mbarki, S., Skalicky, M., Vachova, P., Hajihashemi, S., Jouini, L., Zivcak, M., Tlustos, P., Brestic, M., Hejnak, V. and Khelil, A. Z. 2020. Comparing salt tolerance at seedling and germination stages in local populations of Medicago ciliaris L. to Medicago intertexta L. and Medicago scutellata L. Plants. 9: 526.
Mohamed, A. B., El-Banna, M. F., Farouk, S. and Khafagy. M. A. 2019. The Role of Grain Priming and its Duration on Wheat Germination and Seedling Growth. J. Plant Prod. 10(4): 343-349.
Mondal, S. and Bose, B. 2021. Seed Priming: An Interlinking Technology between Seeds, Seed Germination and Seedling Establishment. In: A. Rustagi, (eds). Plant Reproductive Ecology—Recent Advances. IntechOpen, London.
Mwando, E., Han, Y., Angessa, T. T., Zhou, G., Hill, C. B., Zhang, X. Q. and Li, C. Genome-wide association study of salinity tolerance during germination in barley (Hordeum vulgare L.). 2020. Front. Plant Sci. 11: 118.
Naseer, M. N., Rahman, F. U., Hussain, Z., Khan, I. A., Aslam, M. M., Aslam, A., Waheed, H., Khan, A. U. and Iqbal, S. 2022. Effect of salinity stress on germination, seedling growth, mineral uptake and chlorophyll contents of three cucurbitaceae species. Braz. Arch. Biol. Technol. 65: 1–10.
Nazari, R., Parsa, S., Tavakkol Afshari, R. and Mahmoodi, S. 2020. The effect of seed priming with Salicylic acid on the activity of antioxidant enzymes and lipid peroxidation in deteriorated seeds soybean (Glycine max (L.) Merrill, William variety). Iran. J. Seed Sci.Technol. 9(1): 57-70.
Netondo, G. W., Onyango, J. and Beck, E. 2004. Sorghum and Salinity: I. Response of growth, water relation, and ion accumulation to NaCl salinity. Crop Sci. 44: 797-805.
Omidi, H., Leyla, J. and Hasanali, N. 2014. Seeds of medicinal plants and crops. Natural Res.
Environ. 269-189.
Omidi, H., Sorushzadeh, A., Salehi, A. and Ghezeli, F. 2005. Evaluation of Priming pretreatments on germination rapeseed. Agri. Sci. Technol. 19(2):1-10.
Pagano, A., Macovei, A. and Balestrazzi, A. 2023. Molecular dynamics of seed priming at the crossroads between basic and applied research. Plant Cell Rep. 42: 657–688.
Pequeno, D. N. L., Hernandez-Ochoa, I. M., Reynolds, M., Sonder, K., MoleroMilan, A., Robertson, R. D., Lopes, M. S., Xiong, W., Kropff, M. and Asseng, S. 2021. Climate impact and adaptation to heat and drought stress of regional and global wheat production. Environ. Res. Lett. 16: 054070.
Rashwan, E., Alsohim, A. S., El-Gammaal, A., Hafez, Y. and Abdelaal, K. A. A. 2020. Foliar application of nano zinkoxide can alleviate the harmful effects of water deficit on some flax cultivars under drought conditions. Fresenius Environ. Bull. 29: 8889–8904.
Rehman, H., Iqbal, H., Basra, S. M. A., Afzal, I.., Farooq, M., Wakeel, A. and Ning, W. 2015. Seed priming improves early seedling vigor, growth and productivity of spring maize. J.
Integr. Agric. 14: 1745-1754.
Saadat, H., E. Soltani., and M. Sedghi. 2023a. The effect of seed priming with chitosan on germination characteristics and activity of antioxidant enzymes in rice seedlings (Oryza Sativa L.) under salinity stress. (In Persian, with English Abstract. Plant Pro. Fun. 12(54): 239-258.
Saadat, H., Sedghi, M., Seyed Sharifi, R. and Farzaneh, S. 2023c. The Effect of Priming with Different Levels of Chitosan on Physiological and Biochemical Traits in French Bean (Phaseolus vulgaris L.) Under Salinity Stress. Phant Prod. Technol. 14(2):75-89.
Saadat, T. and Sedghi, M. 2021. Effect of priming and aging on Physiological, biochemical traitsseed common bean (Phaseolus vulgaris L.). J. Seed Res. 11(3): 75-89.
Saadat, T., Alidoost, H. and Sedghi, M. 2022a. The effect of priming and aging on the germination of rice seed masses with different strength. J. Seed Res. 10(4): 60-67.
Saadat, T., Alidoost, H. and Sedghi, M. 2022b. The effect of priming on the activity and gene expression of antioxidant enzymes in rice. J. Seed Res. 11(4): 46-54.
Saadat, T., M. Sedghi, A. Gholipouri, R. Seyed Sharifi, and R. Sheykhbaglou. 2023b. Effect of chitosan on germination indices of common bean (Phaseolus vulgaris) (cv. Sedri) seeds under salt stress. Iran. J. Seed Res. 9(2): 151-162.
Saadat, T., Sedghi, M., Gholipouri, A., Seyed Sharifi, R. and Sheykhbaglou, R. 2020a. The effect of priming deterioration on the activity of antioxidant enzymes and the mobility of seed reserves in French bean (Phaseolus vulgaris L.) cv. Sadri. Iran. J. Seed Sci. Technol. 8(2): 19-32.
Saadat, T., Sedghi, M., Gholipouri, A., Seyed Sharifi, R. and Sheykhbaglou, R. 2020b. Effect of seed priming and aging on germination, biochemical traits and antioxidant enzyme gene expression in common bean (Phaseolus vulgaris l.). Iran. J. Seed Sci. Res. 7(1): 1-13.
Sakhabutdinova, A. R., Fatkhutdinova, D. R., Bezrukova, M.V. and Shakirova, F. M. 2003. Salicylic acid prevents damaging action of stress factors on wheat plants. Bul. J. Plant
Physiol. 314-319.
Salam, A., Ali, A., Afridi, M. S., Ali, S. and Ullah, Z. 2022. Agrobiodiversity: effect of drought stress on the eco-physiology and morphology of wheat. In Biodiversity, Conservation and Sustainability in Asia; Springer: Cham. 597-618.
Seleiman, M. F., Semida, W. M., Rady, M. M., Mohamed, G. F., Hemida, K. A., Alhammad, B. A., Hassan, M. M. and Shami, A. 2020. Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed Cucumber. Plants. 9(12): 1783.
Sneideris, L. C., Gavassi, M. A., Campos, M. L., D'AmicoDamiao, V. and Carvalho, R. F. 2017. Effects of hormonal priming on seed germination of pigeon pea under cadmium stress. An. Acad. Brasil. Cienc. 87:1847-1852.
Souri, M. K. and Tohidloo G. 2021. Effectiveness of different methods of salicylic acid application on growth characteristics of tomato seedlings under salinity. Chem. Biol. Technol. Agric. 6(1): 1-7.
Tahmasbi, B., Ghaderi-Far, F., Sadeghipour, H. R., Galeshi, S. 2015. Enhanced accumulation of fatty acids and lipid hydroperoxides during ageing of sunflower seeds. J. Plant Pro. Fun. 4: 73-83.
Tania, S. S., Rhaman, M. S. and Hossain, M. M. 2020. Hydro-priming and halopriming improve seed germination, yield and yield contributing characters of okra (Abelmoschus esculentus L.). Trop. Plant Res. 7(1): 86-93.
Tao, Q., Yanya Lv, Y., Mo, Q., Bai, M., Han, Y. and Wang, Y. 2018. Impacts of priming on
seed germination and seedling emergence of Cleistogenes songorica under drought stress. J. Seed Sci. Technol. 46(2): 239-258.
TavakolAfshari, R., Rashidi, S. and Alizadeh, H. 2009. Effects of seed aging on germination characteristics and on catalase and peroxidase activities in two canola cultivars (Brassica napus L.). Iran J. Field Crop Sci. 40(2): 125-133.
Tsonev, T. D., Lazova, G. N., Stoinova, Z. G. and Popova, L. P. 1998. A possible rolefor jasmonic acid in adaptation of barley seedling to salinity stress. J. Plant Growth Regul. 17(3):153-159.
Wahid, A., Noreen, A., Basra, S. M., Gelani, S. and Farooq, M. 2008. Priming-induced metabolic changes in sunflower (Helianthus annuus) achenes improve germination and seedling growth. Bot. Studi. 49(2): 343–350.
Wassie, A. T., Zhao, Y. and Boyden, E. S. 2019. Expansion microscopy: principles and uses in biological research. Nature Met. 16(1); 33–41.
Zamani, A., Sadat Nouri, S. A., Tavakol Afshari, R. Iran Nejad, H., Akbari, G. H. A. and Tavakoli, A. 2010. Evaluation of lipid peroxidation and activity of antioxidant enzymes in safflower seed under natural and artificial aging. Iran. J. Crop Sci. 41(3): 545-554.