اثرات عصاره هیدروالکلی دانه زیتون تلخ( Melia azedarach) و چریش(Azadirachta indica) بر روی تعداد Trypanosoma evansi در کبد موش آلودهDOR: 20.1001.1.17359880.1400.14.3.3.4
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستیشهرزاد نصیری سمنانی 1 , نسترن قاسم پور 2
1 - استادیارگروه میکروبیولوژی، دانشکده علوم پایه- مهندسی، واحد زنجان، دانشگاه آزاد اسلامی، زنجان،ایران
2 - دانشجوی دکتری عمومی دامپزشکی، دانشکده دامپیزشکی دانشگاه رازی کرمانشاه، ایران.
کلید واژه: liver, کبد, Trypanosoma evansi, عصاره زیتون تلخ, عصاره چریش, Trypanosoma evansi Steel, Melia azedarach, Azadirachta indica,
چکیده مقاله :
زمینه و هدف: تریپانوزوموز ناشی از Trypanosoma evansi موجب عفونت در پستانداران اهلی و هم چنین انسان می گردد. درمان تریپانوزوموز دارای محدودیت هایی از جمله مقاومت به دارو های شیمیایی می باشد. هدف از مطالعه حاضر مقایسه اثرات عصاره های هیدرو الکلی دانه های چریش و زیتون تلخ در برابر عامل مقاوم این بیماری در کبد موش های آلوده به Trypanosoma evansi است.روش کار: پـس از جمـع آوری و شناسـایی گیاهـان چریش و زیتون تلخ، بـه روش پرکوله از دانه های آن ها عصاره هیدروالکلی تهیه و پس از تغلیظ 100 تا 500 میلی گرم بر کیلو گرم وزن موش به صورت صفاقی به موش های صحرایی نر آلوده شده با 107×3 Trypanosoma evansi به مدت 60 روز تزریق گردید. برای تعیین تعداد پارازیت از روش روتین استفاده شد. آزمایشات آسیب شناسی بافتی کبد بعد از رنگ آمیزی هماتوکسیلن وائوزین انجام و ارزیابی آماری با استفاده از آزمون های آنالیز واریانس ANOVAو با استفاده از نرم افزار Prism انجام گرفت و حد خطای05/0 p< به عنوان اختلاف معنی دار پذیرفته شد.یافته‌ها: عصاره دانه چریش و زیتون تلخ هر دو موجب کاهش تعداد انگل شده ودر مقایسه بین دو عصاره میزان اثر کاهشی زیتون تلخ نسبت به چریش بیشتر بوده است(05/0 p< ). تغییرات احتقان، انحطاط چربی، واکوئولار انحطاط، نکروز و نفوذ سلولی در کبد گروه کنترل مثبت نسبت به گروه های تیمار شده بیشتر بود.نتیجه گیری: استفاده از عصاره های هیدرو الکلی دانه های چریش و زیتون تلخ در مقدار معین داروی گیاهی موثر در بیماری تریپانوزوموز(سوررا) می شود.
Inroduction & Objective: Trypanosoma evansi induced trypanosomiasis causes infection in domestic mammals as well as humans. Treatment of trypanosomiasis has limitations, including resistance to chemical drugs. The aim of this study was to identify active natural products and compare the effects of hydroalcoholic extracts of neem seeds and bitter olives against the resistant agent of this disease in the liver organ of mice infected with Trypanosoma evansi.Material and Methods: After collecting and identifying neem and Bakain seeds plants, hydroalcoholic extract was prepared from their seeds by Perkoleh method and after concentrating 100-500 mg / kg of rat weight peritoneally to male rats infected with 3×107 Trypanosoma evansi were injected for 3 days. Routine method was used to determine the number of noises. Serum levels of ALT, AST, ALP were measured. Tissue pathology tests of. Histopathological liver tests were performed after hematoxylin and eosin staining. Statistical evaluation was performed using ANOVA analysis of variance and Prism software and the error limit of p <0.05 was accepted as a significant difference.Results: Neem and Bakain seeds extracts both reduced the number of parasites and compared to the two extracts, the reducing effect of Bakain was greater than that of Neem. Congestion changes, fat degeneration, vacuolar degeneration, Necrosis and cell infiltration in the liver nephritis was higher of the control groups than the treated groups.Conclusion: The use of neem and Bakain seeds hydroalcoholic extracts of in a certain amount of herbal medicine is effective in trypanosomosis (soda).
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