اثر عصاره هیدروالکلی میوه گیاه زرشک (Berberis vulgaris) بر روند ترمیم زخم باز در موش صحرایی
محورهای موضوعی :
مجله پلاسما و نشانگرهای زیستی
سامان آهنی
1
,
فریبرز معیر
2
,
نفیسه خسروی دهقی
3
1 - دانش آموخته دکترای دامپزشکی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران
2 - گروه پاتوبیولوژی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران
3 - گروه فارماکوگنوزی، دانشکده داروسازی، دانشگاه علوم پزشکی البرز، کرج، ایران
تاریخ دریافت : 1401/11/05
تاریخ پذیرش : 1401/12/26
تاریخ انتشار : 1402/02/01
کلید واژه:
هیستوپاتولوژی",
زرشک",
عصاره هیدروالکلی",
زخم",
",
چکیده مقاله :
زمینه و هدف: عصاره میوه زرشک با داشتن خواص ضدالتهابی و توانایی تسریع فرآیندهای ترمیمی بافت پوششی به عنوان یک گزینه بالقوه برای جایگزینی داروهای موضعی صناعی در درمان زخم‌های پوستی محسوب می‌شود. در مطالعه حاضر تاثیر عصاره هیدروالکلی این میوه بر فاکتورهای موثر بر ترمیم زخم باز در موش‌های صحرایی مورد بررسی قرار گرفت.مواد و روش‌ها: در ناحیه کمری دوازده رت نر برشی تحت بیهوشی ایجاد شد. سپس نمونه‌ها به تعداد مساوی و به طور تصادفی به دو گروه درمان و شاهد تقسیم شدند. به مدت بیست و یک روز، هر دوازده ساعت یکبار، در موضع زخم گروه درمان عصاره هیدروالکلی گیاه زرشک در روغن اوسرین و در گروه شاهد روغن اوسرین به کار برده شد. به منظور بررسی تغییرات ماکروسکوپیک در تمام روزهای مطالعه از سطح زخم عکس‌برداری انجام گرفت. همچنین در روزهای ١، ٣، ٥، ٧، ١٤ و ٢١ از موضع نمونه بافتی تهیه شد و فراسنجه‌های موثر بر ترمیم زخم مورد بررسی قرار گرفت. نتایج: مصرف موضعی ١٥٠ میلی گرم/کیلوگرم عصاره هیدروالکلی میوه زرشک به فاصله هر دوازده ساعت یکبار به طور معناداری بر روند بهبود زخم رت‌ها تاثیر گذاشت. مصرف این دارو باعث کاهش قابل توجه مساحت زخم و فاصله بین دو لبه بافت پوششی و همچنین افزایش رگزایی، تولید جوانه گوشتی، ضخامت بافت اپیتلیوم، سلول‌های فیبروبلاست و کلاژن‌سازی شد. در نهایت جایگزینی کامل بافت ترمیمی توسط بافت طبیعی در گروه درمان مشاهده شد.نتیجه گیری: استفاده از عصاره هیدورالکلی میوه گیاه زرشک تاثیر معنی-داری بر تسریع روند بهبود زخم باز رت‌های نر دارد.
چکیده انگلیسی:
Background and aim: Barberries are well known for their anti-inflammatory properties. The extract of this fruit may help accelerate epithelial tissue recovery; therefore, it could be considered as a potential substitute for synthetic medications. The current study aims to examine the impact of barberries hydro-alcoholic extract on open wound healing pathologic parameters and macroscopic size.Materials and methods: An incision was made in the lumbar region of twelve male rats under anesthesia. They were subsequently divided into treatment and control groups (n=6, each) and were treated with hydro-alcoholic extract of barberries-mixed with eucerin oil, and eucerin oil respectively for twenty-one days; i.e., once every 12 hours. In order to evaluate macroscopic changes in wounds, all rats were photographed daily. Furthermore, pathologic samples were obtained on the 1st, 3rd, 5th, 7th, 14th, and 21st days to investigate the parameters affecting wound healing.Results: The findings show that applying 150 mg/kg hydro-alcoholic extract of barberries has a significant effect on open wound healing in rats. The extract boosted angiogenesis, granulation tissue, epithelial thickness, fibroblast cells, and collagen production in the treatment group while dramatically reducing the macroscopic scar size and epithelial gap. Eventually, the healing skin was thoroughly replaced by normal skin tissue. Conclusion: Hydro-alcoholic extract of barberries (Berberis vulgaris) significantly accelerates the healing process of open wounds in male rats.
منابع و مأخذ:
Mathew-Steiner SS, Roy S, Sen C Collagen in wound healing. Bioengineering. 2021 May; 8(5):63-78
Hobiny A, Alzahrani F, Abbas I, Marin M. The effect of fractional time derivative of bioheat model in skin tissue induced to laser irradiation. Symmetry. 2020 Apr;12(4):602-615
Ciulla TA. Standards of medical care in diabetes. American Diabetes Association. 2009 Jan;32(1):13-61
Daghian SG, Farahpour MR, Jafarirad S. Investigation on acceleration of Staphylococcus aureus and Pseudomonas aeruginosa infected wound healing. Materials Science and Engneering. 2021 Jul;128(2):11-22
Houghton PJ, Woldemariam AI, Khan A, Burke N. Antiviral activity of natural and semi-synthetic chromone alkaloids. Antiviral Research. 1994 Dec;25(3):235-244
Shedoeva A, Leavesley D, Upton Z, Fan C. Wound healing and the use of medicinal plants. Evidence-Based Complementary and Alternative Medicine. 2019 Sep;14(1):23-32
Imenshahidi M, Hosseinzadeh H. Berberine and barberry (Berberis vulgaris): A clinical review. Phytotherapy Research. 2019 Jan;33(3):504-523
Oguntibeju OO. Medicinal plants and their effects on diabetic wound healing. Veterinary World Journal. 2019 May;12(5):653-663
Firdous SM, Sautya D. Medicinal plants with wound healing potential. Bangladesh Journal of Pharmacology. 2018 Feb;13(1):41-52
Kalmarzi RN, Naleini SN, Ashtary-Larky D, Peluso I, Jouybari L, Rafi A, Kooti W. Anti-inflammatory and immunomodulatory effects of barberry (Berberis vulgaris) and its main compounds. Oxidative Medicine and Cellular Longevity. 2019 Nov;14 (2):23-36
Behravan M, Panahi AH, Naghizadeh, A, Ziaee M, Mahdavi R, Mirzapour A. Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. International Journal of Biological Macromolecules. 2018 Nov;124(1):148-154
El-Zahar, KM, Al-Jamaan ME, Al-Mutairi FR, Al-Hudiab AM, Al-Einzi MS, Mohamed AAZ. Antioxidant, Antibacterial, and antifungal activities of the ethanolic extract obtained from Berberis vulgaris roots and leaves. Molecules Journal. 2022 Sep;27(18):14-37
Arefyar R, Miroliaei M, Talebi A, Zahmatkesh M. Histopathological evaluation of wound healing by extracts from Glaucium corniculatum. Iran Biology Journal. 2018 Sep.31(1):24-35
Walker H.L., Mason A.D. A standard for animal skin burn. Journal of Trauma. 1996 Jul;8(10):49-51
Li C, Li J. Histological observation of the healing course of superficial third degree burn wounds treated with MEBT-MEBO. Chinese journal of burns wound and surface ulcers. 2003 Sep;15(16):89-98
Mohebali S, Nasri S, Kamalinejhad M, Noori A. Antionociceptive and anti-inflammatory effects of Berberis vulgaris root's hydroaicoholic extract and determination of it's possible antinociceptive mechanism in male mice. Archives of Advances in Biosciences. 2011 Jan;2(4):83-92
Mousavi ZB, Meshki M, Hemmati, A, Veisi MS, Rafiei, R. Evaluation of the efficacy of Quince mucilage on wound healing. Iranian Journal of Dermatology. 2006 Jan;9(37):260-263
Mazumder PM, Saumya D, Das Sanjita, Das M anas Kumar, Champion RH, Burton JL, Breathnach SM. Textbook of Dermatology. Oxford: Blackwell Science. Publishing; 1998.p.337-339
Mizuno K, Wachi H, Isogay Z, Yoneda M. Proteolytic release of latent transforming growth factor (TGF-B) binding fragment in wound healing. Health science. 2009 Jun;55(3):468-472
Sewall GK, Robertson KM, Connor NP, Heisey DM, Hartig GK. Effect of topical mitomycin on skin wound contraction. Arch Facial Plast Surge. 2003 Jan;5(1):59-62
Shiravi N, Alebooyeh M, Hojati V, Akbari H. The effect of extract of Henna leaf (Lawsonia inermis) on skin wound healing in Wistar rats. Biology journal of Azad university of Damghan. 2011 Aug;3(4):107-116
Nabiuni M, Azarnia M, Mousavi R, Ramezani T. The effect of Myrtus Communism leaf extract cream on wound healing process in Wistar rats. Cmja. 2014 Nov;4(3):854-864
Asif A, Kakub G, Mehmood S, Khunum R, Gulfraz M. Wound healing activity of root extracts of Berberis lyceum royle in rats. Phytotherapy Research. 2007 Jun;21(6):589-591
Peraza S, Chai H, Shin Y, Santisuk T, Reutrakul V, Farnsworth N, Cordell G, Pezzuto J, Kinghorn A. Constituents of the leaves and twigs of Ficus hispida. Planta Med. 2002 Sep;68(2):186-188
Pashaee M, Shiravi A, Hojati V. The effect of hydroalcoholic extract of Berberis vulgaris on wound healing of diabetic wistar rats. Chemical Health Risks. 2016 Jul;6(4):319-325
Kazemi A, Talari S, Hooshyar H. The effect of an alcoholic extract of Berberis Vulgaris on Cutaneous leishmaniasis ( major) in BALB/c mice. School of public health and institute of public health research. 2007 Sep;5(3):35-42(Persian)
Seyed Ahmadi SG, Farahpour MR, Hamishehkar H. Topical application of Cinnamon verum essential oil accelerates infected wound healing process by increasing tissue antioxidant capacity and keratin biosynthesis. The Kaohsiung Journal of Medical Sciences. 2019 Jul;35(11):686-694
Daemi A, Farahpour MR, Oryan A, Karimzadeh S, Tajer E. Topical administration of hydroethanolic extract of Lawsonia inermis (henna) accelerates excisional wound healing process by reducing tissue inflammation and amplifying glucose uptake. The Kaohsiung Journal of Medical Sciences. 2019 Jan;35(1):24-32
Daemi A, Lotfi M, Farahpour MR, Oryan A, Ghayour SJ, Sonboli A. Topical application of Cinnamomum hydroethanolic extract improves wound healing by enhancing re-epithelialization and keratin biosynthesis in streptozotocin-induced diabetic mice. Pharmaceutical Biology. 2019 Dec;57(1):799-806
Modarresi M, Farahpour MR, Baradaran B. Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model. Inflammopharmacology. 2019 Jun;27(3):531-537
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Mathew-Steiner SS, Roy S, Sen C Collagen in wound healing. Bioengineering. 2021 May; 8(5):63-78
Hobiny A, Alzahrani F, Abbas I, Marin M. The effect of fractional time derivative of bioheat model in skin tissue induced to laser irradiation. Symmetry. 2020 Apr;12(4):602-615
Ciulla TA. Standards of medical care in diabetes. American Diabetes Association. 2009 Jan;32(1):13-61
Daghian SG, Farahpour MR, Jafarirad S. Investigation on acceleration of Staphylococcus aureus and Pseudomonas aeruginosa infected wound healing. Materials Science and Engneering. 2021 Jul;128(2):11-22
Houghton PJ, Woldemariam AI, Khan A, Burke N. Antiviral activity of natural and semi-synthetic chromone alkaloids. Antiviral Research. 1994 Dec;25(3):235-244
Shedoeva A, Leavesley D, Upton Z, Fan C. Wound healing and the use of medicinal plants. Evidence-Based Complementary and Alternative Medicine. 2019 Sep;14(1):23-32
Imenshahidi M, Hosseinzadeh H. Berberine and barberry (Berberis vulgaris): A clinical review. Phytotherapy Research. 2019 Jan;33(3):504-523
Oguntibeju OO. Medicinal plants and their effects on diabetic wound healing. Veterinary World Journal. 2019 May;12(5):653-663
Firdous SM, Sautya D. Medicinal plants with wound healing potential. Bangladesh Journal of Pharmacology. 2018 Feb;13(1):41-52
Kalmarzi RN, Naleini SN, Ashtary-Larky D, Peluso I, Jouybari L, Rafi A, Kooti W. Anti-inflammatory and immunomodulatory effects of barberry (Berberis vulgaris) and its main compounds. Oxidative Medicine and Cellular Longevity. 2019 Nov;14 (2):23-36
Behravan M, Panahi AH, Naghizadeh, A, Ziaee M, Mahdavi R, Mirzapour A. Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. International Journal of Biological Macromolecules. 2018 Nov;124(1):148-154
El-Zahar, KM, Al-Jamaan ME, Al-Mutairi FR, Al-Hudiab AM, Al-Einzi MS, Mohamed AAZ. Antioxidant, Antibacterial, and antifungal activities of the ethanolic extract obtained from Berberis vulgaris roots and leaves. Molecules Journal. 2022 Sep;27(18):14-37
Arefyar R, Miroliaei M, Talebi A, Zahmatkesh M. Histopathological evaluation of wound healing by extracts from Glaucium corniculatum. Iran Biology Journal. 2018 Sep.31(1):24-35
Walker H.L., Mason A.D. A standard for animal skin burn. Journal of Trauma. 1996 Jul;8(10):49-51
Li C, Li J. Histological observation of the healing course of superficial third degree burn wounds treated with MEBT-MEBO. Chinese journal of burns wound and surface ulcers. 2003 Sep;15(16):89-98
Mohebali S, Nasri S, Kamalinejhad M, Noori A. Antionociceptive and anti-inflammatory effects of Berberis vulgaris root's hydroaicoholic extract and determination of it's possible antinociceptive mechanism in male mice. Archives of Advances in Biosciences. 2011 Jan;2(4):83-92
Mousavi ZB, Meshki M, Hemmati, A, Veisi MS, Rafiei, R. Evaluation of the efficacy of Quince mucilage on wound healing. Iranian Journal of Dermatology. 2006 Jan;9(37):260-263
Mazumder PM, Saumya D, Das Sanjita, Das M anas Kumar, Champion RH, Burton JL, Breathnach SM. Textbook of Dermatology. Oxford: Blackwell Science. Publishing; 1998.p.337-339
Mizuno K, Wachi H, Isogay Z, Yoneda M. Proteolytic release of latent transforming growth factor (TGF-B) binding fragment in wound healing. Health science. 2009 Jun;55(3):468-472
Sewall GK, Robertson KM, Connor NP, Heisey DM, Hartig GK. Effect of topical mitomycin on skin wound contraction. Arch Facial Plast Surge. 2003 Jan;5(1):59-62
Shiravi N, Alebooyeh M, Hojati V, Akbari H. The effect of extract of Henna leaf (Lawsonia inermis) on skin wound healing in Wistar rats. Biology journal of Azad university of Damghan. 2011 Aug;3(4):107-116
Nabiuni M, Azarnia M, Mousavi R, Ramezani T. The effect of Myrtus Communism leaf extract cream on wound healing process in Wistar rats. Cmja. 2014 Nov;4(3):854-864
Asif A, Kakub G, Mehmood S, Khunum R, Gulfraz M. Wound healing activity of root extracts of Berberis lyceum royle in rats. Phytotherapy Research. 2007 Jun;21(6):589-591
Peraza S, Chai H, Shin Y, Santisuk T, Reutrakul V, Farnsworth N, Cordell G, Pezzuto J, Kinghorn A. Constituents of the leaves and twigs of Ficus hispida. Planta Med. 2002 Sep;68(2):186-188
Pashaee M, Shiravi A, Hojati V. The effect of hydroalcoholic extract of Berberis vulgaris on wound healing of diabetic wistar rats. Chemical Health Risks. 2016 Jul;6(4):319-325
Kazemi A, Talari S, Hooshyar H. The effect of an alcoholic extract of Berberis Vulgaris on Cutaneous leishmaniasis ( major) in BALB/c mice. School of public health and institute of public health research. 2007 Sep;5(3):35-42(Persian)
Seyed Ahmadi SG, Farahpour MR, Hamishehkar H. Topical application of Cinnamon verum essential oil accelerates infected wound healing process by increasing tissue antioxidant capacity and keratin biosynthesis. The Kaohsiung Journal of Medical Sciences. 2019 Jul;35(11):686-694
Daemi A, Farahpour MR, Oryan A, Karimzadeh S, Tajer E. Topical administration of hydroethanolic extract of Lawsonia inermis (henna) accelerates excisional wound healing process by reducing tissue inflammation and amplifying glucose uptake. The Kaohsiung Journal of Medical Sciences. 2019 Jan;35(1):24-32
Daemi A, Lotfi M, Farahpour MR, Oryan A, Ghayour SJ, Sonboli A. Topical application of Cinnamomum hydroethanolic extract improves wound healing by enhancing re-epithelialization and keratin biosynthesis in streptozotocin-induced diabetic mice. Pharmaceutical Biology. 2019 Dec;57(1):799-806
Modarresi M, Farahpour MR, Baradaran B. Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model. Inflammopharmacology. 2019 Jun;27(3):531-537
