اثر ترمیم کنندگی عصاره آبی- الکلی کرم خاکی بر زخم پوستی در ماهی طلایی (Carassius auratus gibelio)

محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی

مهران عربی ¹ , علیرضا شمس آبادی ²

1 - گروه علوم جانوری٬ دانشکده علوم پایه٬ دانشگاه شهرکرد٬ شهرکرد، ایران
2 - گروه علوم جانوری٬ دانشکده علوم پایه٬ دانشگاه شهرکرد٬ شهرکرد، ایران.

تاریخ دریافت : 1401/10/30 تاریخ پذیرش : 1401/12/08 تاریخ انتشار : 1402/02/01

کلید واژه: زخم, کرم خاکی, ماهی,

چکیده مقاله :

زمینه و هدف: فرآیند ترمیم زخم شامل مجموعه‌ای از روندهای برنامه‌ریزی شده مولکولی و سلولی است. هدف از انجام مطالعه حاضر ارزیابی توان ترمیم‌کنندگی عصاره آبی- الکلی کرم‌‌های خاکی (EBE) در بهبودی زخم تجربی در پوست ماهیان طلایی (Carassius auratus gibelio) بوده است. مواد و روش‌ها: ماهیان طلایی (با سه تکرار مستقل و 14 قطعه در هر گروه) برای یک دوره 14روزه˓ به 5 گروه تقسیم-بندی شدند: 1) شاهد منفی (بدون زخم)٬ 2) شاهد کاذب (شم): زخم با تیمار حلال EBE: مخلوط روغن کرچک و وازلین سفید٬ 3) شاهد مثبت: زخم با تیمار پماد فنی‌تویین سدیم 1 درصد˓ 4) زخم با تیمار پماد حاوی 1 میلی‌گرم از EBE٬ 5) زخم با تیمار پماد حاوی 10 میلی‌گرم از EBE. در ادامه˓ ارزیابی‌های ماکروسکوپی و میکروسکوپی˓ سنجش میزان کلاژن (محتوی هیدروکسی‌پرولین) در پوست˓ همراه با تعیین فعالیت لیزوزیمی در سرم خون ماهیان به انجام رسید.نتایج: بررسی نتایج نشان داد که تیمار 1 میلی‌گرم از EBE˓ موجب افزایش قابل ملاحظه‌‌ای در قطر اپیدرم٬ سنتز و آرایش رشته‌های کلاژن˓ کاهش التهاب در درم˓ و نیز در میزان هیدروکسی‌پرولین پوست و فعالیت لیزوزیمی سرم خون نسبت به سایر گروه‌ها شده است.نتیجه‌گیری: نتایج ما نشان داد که کاربرد EBE با دوز 1 میلی‌گرم در گرم می‌تواند به عنوان یک ترکیب دارویی موثر در ترمیم زخم پوستی در جانوران به ویژه آبزیان مورد استفاده قرار گیرد.

چکیده انگلیسی:

Background & aim: Wound healing is a complex programmed sequence of cellular and molecular processes. The objective of the present study was to evaluate the ability of earthworm’s hydro-alcoholic body extract (EBE) to heal the experimental skin wound in goldfish (Carassius auratus gibelio).Materials and Methods: Fish (independent triplicate, 14/group), for 14 days, were divided into 5 groups 1) negative control (Unwounded); 2) pseudo-control (sham): wound treated with EBE carrier (mixture of castor oil and white vaseline); 3) Positive control: wound treated with sodium phenytoin 1%; 4) 1 mg/gr EBE-treated wound, 5) 10 mg/gr EBE-treated wound. In this study, macroscopic and microscopic evaluations and collagen turnover (Hydroxyproline content) in the skin, along with determination of lysozyme activity in blood sera were carried out. Results: In fish in group 4 (1 mg/gr EBE-treated wound), an increase in the epithelium diameter, good presence and appropriate synthesis of collagen with and low edema in dermis; increased hydroxyproline content; and elevated blood lysozyme activity were observed comparing to other groups.Conclusion: Our results revealed that application of 1 mg/gr EBE might be as an effective medicinal material in the process of animal wound healing particularly in aquatics.

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