اثر حفاظتی عصاره هیدروالکلی گل ساعتی (Passifloracaerulea) بر علیه سوء عملکرد کبدی القاء شده توسط کلرید کادمیوم در موشهای صحرایی نر بالغ
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی
مهرداد شریعتی
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مهرنوش قوامی
2
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مختار مختاری
3
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سعید خاتم ساز
4
1 - گروه زیست شناسی،واحدکازرون ،دانشگاه آزاد اسلامی،کازرون،ایران
2 - گروه زیست شناسی،واحدکازرون ،دانشگاه آزاد اسلامی،کازرون،ایران
3 - گروه زیست شناسی،واحدکازرون ،دانشگاه آزاد اسلامی،کازرون،ایران
4 - گروه زیست شناسی،واحدکازرون ،دانشگاه آزاد اسلامی،کازرون،ایران
کلید واژه: سوء عملکرد کبدی, کلرید کادمیوم, گل ساعتی, موشهای صحرایی نربالغ,
چکیده مقاله :
زمینه و هدف: کلرید کادمیوم باعث ایجاد صدمه کبدی می گردد. در این مطالعه اثر حفاظتی عصاره هیدروالکلی گل ساعتی بر علیه سوء عملکرد کبدی القاء شده توسط کلرید کادمیوم در موش های صحرایی نر بالغ مورد بررسی قرارگرفت. روش کار: 54 سر موش صحرایی نربالغ نژاد ویستار به6 گروه 9 تایی تقسیم شدند. گروه کنترل،گروه شاهد1: 0.2ml/kg آب مقطر روزانه به صورت درون صفاقی به عنوان حلال دریافت کردند.گروه شاهد2: 2mg/kg کلرید کادمیوم روزانه به صورت درون صفاقی به مدت 21 روز دریافت کردند.گروه های تجربی3و2و1: 2mg/kg کلرید کادمیوم روزانه به صورت درون صفاقی به مدت 21 روز و سپس 150,300,450mg/kg عصاره هیدروالکلی بخش های هوایی گل ساعتی روزانه به صورت درون صفاقی به مدت30روز دریافت کردند. سطوح سرمی ALT,AST,ALP,GGT,LDH،آلبومین، بیلی روبین و پروتئین توتال اندازه گیری شدند. آزمایشات آسیب شناسی بافتی کبد بعد از رنگ آمیزی هماتوکسیلن وائوزین انجام شد. یافته‌ها: میانگین غلظت سرم ALT، ALp ،LDH ، GGT و بیلی روبین در تمام گروه های تجربی دریافت کننده کلرید کادمیوم و عصاره هیدروالکلی بخش های هوایی گل ساعتی و کلرید کادمیوم نسبت به گروه دریافت کننده کلرید کادمیوم کاهش معنی داری نشان داد. میانگین غلظت سرم AST در گروه های تجربی دریافت کننده 3و2 نسبت به گروه دریافت کننده کلرید کادمیوم کاهش معنی داری نشان داد. میانگین غلظت پروتئین توتال و آلبومین سرم در گروه های تجربی 3و2 نسبت به گروه دریافت کننده کلرید کادمیوم افزایش معنی داری نشان داد(05/0>p ). نتیجه‌گیری: نتایج این مطالعه نشان داد که عصاره هیدروالکلی بخش های هوایی گل ساعتی دارای اثر حفاظتی بر سوء عملکردکبدی القاء شده توسط کلرید کادمیوم در موش های صحرایی نر بالغ می باشد.
Background:Cadmium chloride causes liver injury. In this study, the protective effect of hydroalcoholic extract of Passifloracaerulea aerial parts against hepatic dysfunction induced by cadmium chloride in adult male rats was investigated. Material and Methods:Fifty-four adult male rats were divided into 6 groups of 9:control group; sham group1:received 0.2ml/kg distilled water daily intraperitoneally as solvent. Sham group2;received 2 mg/kg cadmium chloride daily intraperitoneally during 21 days; experimental groups 1, 2 and 3: received 2 mg/kg cadmium chloride daily intraperitoneally during 21 days and then the hydroalcoholic extract of Passifloracaerulea aerial parts at the doses of 150, 300, 450mg/kg intraperitoneally during 30 days . The serum levels of AST, ALT, ALP, GGT, LDH, albumin, bilirubin, and total protein were measured. The pathological examination of hepatic tissue samples were done after hematoxylin - eosin staining. Results:The mean levels of ALT, ALP, GGT, LDH and bilirubin in the all experimental groups showed a significant decrease compared to the group receiving cadmium chloride. The mean levels of AST in the experimental groups 2 and 3 showed a significant decrease compared to the group receiving cadmium chloride. The mean serum albumin and total protein concentration in experimental groups 2and 3 increased significantly compared to the group receiving cadmium chloride. In all experimental groups the hepatic tissue changes induced by cadmium chloride improved which were dose dependent (P˂0.05). Conclusion:The results of this study showed that the hydroalcoholic extract of Passifloracaerulea aerial parts had protective effects against the hepatic dysfunction induced by cadmium chloride in adult male rats.
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