تاثیر عصاره گیاه زیرفون(Tilia platyphyllos L.) بر میزان هورمون های جنسی و تغییرات هیستوپاتولوژیک در موش های صحرایی نر سالم و مبتلا به واریکوسل
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستیریحانه محبعلی 1 , اکرم عیدی 2 , پژمان مرتضوی 3 , محمد امین عدالت منش 4
1 - گروه زیست شناسی، دانشکده علوم پایه، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران
3 - گروه پاتولوژی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران
4 - دانشیار گروه زیست شناسی، دانشکده علوم پایه، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
کلید واژه: موش صحرایی, واریکوسل, زیرفون, بیضه,
چکیده مقاله :
زمینه و هدف: واریکوسل یکی از شایع ترین علل ناباروری در مردان دارد. مطالعات گوناگونی در خصوص درمان واریکوسل و تغییرات هورمونی بیضه ها صورت گرفته است. یکی از درمان های غیرتهاجمی واریکوسل درمان با گیاه زیرفون(Tilia platyphyllos L.) به عنوان یک دارویی در درمان زخم معده، اسپاسم و آرام بخش استفاده می شود. ترکیبات شیمیایی همانند ترپنوئیدها و فلاونوئیدها در گیاه زیرفون شناسایی شده است. هدف بررسی اثر عصاره گیاه زیرفون بر آسیب شناسی بیضه موش های صحرایی سالم و واریکوسلی انجام گرفت. روش کار: در این تحقیق 48 سر موش صحرایی به صورت تصادفی در 8 گروه تقسیم شدند: کنترل سالم، گروه های سالم دریافت کننده عصاره زیرفون (50، 100 و 200 میلی گرم بر کیلوگرم وزن بدن دریافت خوراکی، روزانه)، کنترل نابارور(جراحی واریکوسل)، گروه های واریکوسلی همراه با عصاره زیرفون(50، 100 و 200 میلی گرم بر کیلوگرم وزن بدن دریافت خوراکی، روزانه). پس از 60 روز خون گیری و آزمایشات هورمونی و بافتی بیضه انجام شد و داده ها با آنالیز واریانس یک عاملی ارزیابی شدند. یافته ها: نتایج نشان داد که میزان هورمون تستوسترون کاهش و میزان هورمون LH و FSH افزایش معناداری در حیوانات کنترل واریکوسل در مقایسه با کنترل سالم نموده است. تیمار عصاره زیرفون از تغییرات هورمون های جنسی جلوگیری کرده و سبب بهبود معناداری در میزان هورمون های جنسی و بافتی حیوانات نابارور شده است. نتیجه گیری: ظاهرا زیرفون به عنوان عامل آنتی اکسیدانی در برابر آسیب های القاء واریکوسل در موش صحرایی عمل می کند.
Background and Aim: Varicocele is one of the most common causes of infertility in men. Various studies have been performed on the treatment of varicocele and hormonal changes in the testicles. One of the non-invasive treatments for varicocele is treatment with Tilia platyphyllos L. as a medicine used to treat stomach ulcers, spasms and sedatives. Chemical compounds such as terpenoids and flavonoids have been identified in the linden. The aim of this study was to evaluate the effect of linden extract on testicular pathology of healthy and varicocele rats.Materials and Methods: In this study, 48 rats were randomly divided into 8 groups: healthy control, healthy groups receiving linden extract (50, 100 and 200 mg / kg intragastrically, daily) alone, infertile control (varicocele surgery), varicocele groups together with linden extract (50, 100 and 200 mg / kg intragastrically, daily). After 60 days, serum was obtained and hormonal assays and histopathological examinations were performed and the data were analyzed using one-way analysis of variance.Results: The results showed that the level of testosterone decreased and levels of LH and FSH increased significantly in varicocele control animals compared with healthy controls. َAlso, administration of linden extract has prevented hormonal changes and has significantly improved the sex hormones level and histological index in varicocele rats.Conclusion: The linden extract acts as a potent antioxidant against varicocele-induced damage in rats.
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