مقایسه اثر عصاره‌ی هیدروالکلی شیرین‌بیان (Glycyrrhiza glabra) و ماده‌ی موثره‌ی آن گلیسریزیک اسید بر میزان پروتئین‌های Bax و Bcl-2 در سلول‌های کبد موش‌های صحرایی نر دیابتی شده‌‌ی نوع یک
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی
صدیقه جانی
1
,
ویدا حجتی
2
,
غلامحسن واعظی
3
,
راهله رهباریان
4
1 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
2 - گروه زیست‌شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران.
3 - گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی،دامغان،ایران
4 - گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران
کلید واژه: BAX, آلوکسان, BCL2, موش صحرایی, شیرین‌بیان,
چکیده مقاله :
زمینه و هدف: عملکرد کبد وابسته به انسولین است و به شدت تحت تأثیر دیابت قرار می‌گیرد. دیابت از طریق استرس اکسیداتیو می تواند منجر به آسیب کبد شود. مطالعه حاضر به منظور بررسی اثرات احتمالی عصاره‌ی هیدروالکلی شیرین‌بیان (Glycyrrhiza glabra) و گلیسریزیک‌اسید بر فاکتورهای آپوپتوزی Bax و Bcl2 در موش‌های صحرایی نر دیابتی نوع 1 انجام شد. روش کار: در این مطالعه تجربی، 72 سر موش صحرایی نر نژاد ویستار به گروه‌های شاهد سالم، شاهد دیابتی و گروه‌های دیابتی تحت تیمار با عصاره‌ی هیدروالکلی شیرین‌بیان و گلیسریزیک‌اسید با غلظت های 50 و 100 میلی‌گرم بر کیلوگرم از طریق تزریق داخل صفاقی در بازه زمانی 15 و 30 روز، تقسیم شدند. گروه‌های شاهد دیابتی و تیمار، با یک بار تزریق داخل صفاقی به میزان 120 میلی‌گرم بر کیلوگرم آلوکسان مونو هیدرات دیابتی شدند. در پایان دوره تیمار فاکتورهای آپوپتوزی Bax و Bcl2، با روش الایزا سنجیده شد. نتایج توسط نرم افزار SPSS 21 و آنالیز واریانس یک طرفه و آزمون تعقیبی توکی تحلیل شد. یافته ها: فاکتور آپوپتوزی Bax در گروه های تحت تیمار در مقایسه با گروه‌های شاهد دیابتی به‌طور معنی‌داری کاهش و فاکتور ضدآپوپتوزی Bcl2 به طور معنی داری افزایش یافت(05/0 p≤) که نشانگر بهبود ساختار بافت آسیب دیده ی کبد در گروه‌های تیمار است. نتیجه گیری: عصاره‌ی هیدروالکلی گیاه شیرین‌بیان وگلیسریزیک‌اسید در دوزهای بیان شده‌‌ی ضد آپوپتوز می‌باشد و در برابر آسیب‌های کبدی القاشده‌‌ی توسط دیابت اثر محافظتی دارد.
Introduction and Objective: The function of the liver as an insulin-dependent organ is strongly affected by diabetes. Diabetes can lead to liver damage through oxidative stress. The present study was performed to evaluate the possible effects of hydroalcoholic extract of licorice (Glycyrrhiza glabra) and glyceric acid on Bax and Bcl2 apoptotic factors in male type 1 diabetic rats. Material and Methods: In this experimental study, 72 male Wistar rats were injected into diabetic control, diabetic control and diabetic groups treated with hydroalcoholic extract of licorice and glycyrrhizinic acid at concentrations of 50 and 100 mg / kg by injection. The time intervals of 15 and 30 days were divided. Diabetic control and treatment groups became diabetic with a single intraperitoneal injection of 120 mg/kg alloxane monohydrate. At the end of the treatment period, Bax and Bcl2 apoptotic factors were measured. The results were analyzed by SPSS 21 software and one-way analysis of variance and Tukey post hoc test. Results: Bax apoptotic factor was significantly decreased in the treated groups compared to the diabetic control groups and Bcl2 anti-apoptotic factor was significantly increased (p <0.05) which indicates improvement in the structure of damaged liver tissue in the groups. It is a treatment. Conclusion: Hydroalcoholic extract of licorice and glycyrrhizic acid is in expressed doses of anti-apoptotic and has a protective effect against liver damage induced by diabetes.
1.Aliparasti, M., Dadkhah, M., Alipour, M., Almasi, Sh., Faizi, H. (2016). The effect of ghrelin on Bcl-2Bax gene expression in lung tissue of rats kept under chronic hypoxia. Scientific Research Journal of Zanjan University of Medical Sciences, 24(106); 89-79.
2.Amani, M., Gharebagh R. S., N. Mostaoufi. (2005). Optimal extraction of glycyrrhetinic acid from licorice root. Journal of Food Technology, 3(4); 576-580.
3.Amouoghli-Tabrizi, B., Mohajeri, D. (2010). Protective effect of edible turmeric (Curcuma longa Linn.) powder on early hepatic injury in diabetic rats. Journal of Kashan Uniersity of Medical Sciences (Feyz), 14(3); 190-199.
4.Ataei, Gh, Rahbarian, R. (2020). Investigating the effect of safranal on Bax and Bcl2 and oxidative stress levels in testis tissue of streptozotocin-induced diabetic rats. Journal of Kashan Uniersity of Medical Sciences (Feyz), 24(1); 10-20.
5.Batiha, G., Beshbishy, A.M., Mleeh, A.E., Daim, M.M., Devkota, H.P. (2020). Traditinal uses bioactive chemical constituents, and pharmacological and toxicological activities of Glycyherriza glabral (Fabacea). Biomolicules, 10; 325.
6.Bugianesi, E., McCullough, A.J., Marchesini, G. (2005). Insulin resistance: a metabolic pathway to chronic liver disease. Hepatology, 42(5); 987-1000.
7.Burkart, V., Wang, Z.Q., Radons, J., Heller, B. (1999). Mice lacking the poly (ADP-ribose) polymerase gene are resistant to pancreatic beta-cell destruction and diabetes development induced by streptozocin. Nature Medicine, 5; 314-319.
8.Chen, Z.W., Chen, L.Y., Dai, H.L., Chen, J.H., Fang, L.Z. (2008). Relationship between alanine aminotransferase levels and metabolic syndrome in nonalcoholic fatty liver disease. Journal of Zhejiang University Science, 9(8); 616-622.
9.Chin, Y.W., Balunas, M.J., Chai, H.B., Kinghorn, A.D. (2006). Drug discovery from natural sources. The AAPS Journal, 8(2); E239-E253.
10.Darabi, S, Hasanvand, A, Norohahi, A. (2016). Evaluation of anti-inflammatory effects of hydroalcoholic extracts of garlic, nettle leaves and olive in rats with diabetes. C.M.J., 6(S1); 1452-1460.
11.Dunn, J.S., Kirkpatrick, J., McLetchie, N.G.B., Telfer, S.V. (1943). Necrosis of the islets of Langerhans produced experimentally. Journal of Pathology and Bacteriology, 55; 245-257.
12.Ebadollahi, S., Pouramir, M., Zabihi, E., Golpour, M., Aghajanpour-Mir, M. (2021). The effect of arbutin on the expression of tumor suppressor P53, BAX/BCL-2 ratio and oxidative stress induced by tert-butyl hydroperoxide in fibroblast and lncap cell lines. Cell Journal, 22(4); 532-541.
13.Eiselein, L., Schwartz, H.J., Rutledge, J.C. (2004). The challenge of type 1 diabetes mellitus. ILAR Journal, 45(3); 231-236.
14.Fernández-Mejía, C., Lazo-de-la-Vega-Monroy, M.L. (2013). Oxidative stress in diabetes mellitus and the role of vitamins with antioxidant actions. DOI: 10.5772/51788.
15.Ganda, O.P., Rossi, A.A., Like, A.A. (1976). Studies on streptozotocin diabetes. Diabetes, 25; 595-603.
16.Gissen, P., Arias, I.M. (2015). Structural and functional hepatocyte polarity and liver disease. Journal of Hepatology, 63(4); 1023-1037.
17.Heydari, F., Mirazi, N. (2016). Effect of hydro-alcholic extract of Pelargonium graveolens L. on serum lipid profile in male rat. Feyz, 20(3); 196-204.
18.Hye-won, R., Ji-Na, L., Hyung-Rhokim, M. (2000). Protective mechanism of glucose against alloxan–induced B-cell damage. Experimental and Molecular Medicine, 32(1); 12-17.
19.Jabbari, S.E., Gholami, M., Nikbakht, H., Shakeri, N., Ghazalian, F. (2019). Effect of aerobic training and L-carnitine supplementation on some apoptotic factors in diabetic rat liver. Razi Journal of Medical Sciences, 26(7); 131-134
20.Jamaluddin, J.L., Huri, H.Z., Vethakkan, S.R. (2016). Clinical and genetic predictors of dipeptidyl peptidase-4 inhibitor treatment response in Type 2 diabetes mellitus. Pharmacogenomics, 17(8); 867-881.
21.Kapoor, R., Kakkar, P. (2014). Naringenin accords hepatoprotection from streptozotocin induced diabetes in vivo by modulating mitochondrial dysfunction and apoptotic signaling cascade. Toxicology Reports, 1; 569-581.
22.Karimi, M.N., Abbasalipourkabir, R., Arab Sadeghabadi, Z., Ziamajidi, N. (2017). The level of gene expression of Bax and Bcl-2 and the activity of caspase 3 in the liver tissues of normal, type 1 and type 2 diabetic rats before and after treatment with aqueous extract of garlic. JSS Academy of Higher Education and Research, 25(7); 547-555
23.Ketabchi, S., Papari Moghadamfard, M. (2021). Medicinal plants effective in the prevention and control of coronaviruses (Persian). Complementary Medicine Journal, 10(4); 296-307.
24.Khameneh AR, Jafari A, Nikookheslat S, Karimi P. (2020). Effect of chronic caffeine administration on expression ratio of Bax and Bcl-2 proteins in myocardial tissue of male wistar rats with type 2 diabetes (Persian). Complementary Medicine Journal, 10(3); 206-217.
25.Khanahmadi, M.M., Naghdi Badi, H., Akhondzadeh, S., Khalighi-Sigaroodi, F., Mehrafarin, A., Shahriari, S. (2013). A Review on medicinal plant of Glycyrrhiza glabra L. Journal of Medicinal Plants, 12(46), 1-12.
26.Khoshnam, S.E., Farzaneh, M., Valipour, M., Bahaoddini, A., Valipour, A. (2015). Review of the phytochemical, pharmacological and physiological properties of Licorice (Glycyrrhiza glabra). Clinical Excellence, 4(S1); 71-56
27.Kharroubi, A.T., Darwish, H.M. (2015). Diabetes mellitus: The epidemic of the century. World Journal of Diabetes, 6(6); 850.
28.Kroncke, K.D., Fehsel, K., Sommer, A., Rodriguez, M.L. (1995). Nitric oxide generation during cellular metabolization of the diabetogenic N-methyl-N-nitroso-urea streptozotocin contributes to islet cell DNA damage. BiolChem Hoppe-Seyler, 376; 179-185.
29.Lachin, T., Reza, H. (2012). Anti-diabetic effect of cherries in alloxan induced diabetic rats. Recent Patents on Endocrine, Metabolic and Immune Drug Discovery, 6(1); 67-72.
30.Marzetti, E., Privitera, G., Simili, V., Wohlgemuth, S.E., Aulisa, L., Pahor, M., (2010). Multiple pathways to the same end: mechanisms of myonuclear apoptosis in sarcopenia of aging. The Scientific World Journal, 10; 340-349. 31.Mohammadi Malayeri, M.R., Hesaraki, S., Jamal Livani, P. (2014). Protective effect of Iranian black tea on hepatotoxicity induced by cadmium chloride in rats. Comparative Pathology, 11(4); 1481-1490.
32.Mohammad-Sadeghi, H., Mansorabadi, A.H, Emami, S., Nahvinezhad, M.R, Mogoii, M. (2015). The effect of hydroalcoholic extract of mountain cockatiel on the number Active pancreatic beta cells induced in type 1 diabetic mice Streptozotocin. Iranian Journal of Diabetes and Metabolism, 14(6); 373-378.
33.Mukim, M., Sharma, P., Mohsina, F.P., Faheem, I.P., Kukkar, R., Patel, R. (2021). Multiple used medicinal plant: glycyrrhiza glabra– review. SunText Review of Pharmaceutical Sciences, 2(2); 113.
34.Nasiri Asl, M., Hosseinzadeh, H. (2007). A review of the antiviral effects of licorice and its active ingredient glycerin. Journal of Medicinal Plants, 6(22); 1-12.
35.Petrovska, B.B. (2012). Historical review of medicinal plants’ usage. Pharmacognosy Review, 6(11); 1-5.
36.Qureshi, J.A., Memon, Z., Ismail, K., Saher, F., Motiani, V., Mushtaq, Z. (2020). Anti-hyperglycemic and anti-dyslipidemic activities of Glycyrrhiza glabra root extract in diabetic rats. Journal of Islamic International Medical College (JIIMC), 15(2); 98-103.
37.Rahbarian, R., Sadoughi, S.D. (2017). Effect of catechin on serum levels of inflammatory cytokines, activity of antioxidant enzymes, oxidative DNA damage of ovarian tissue in rats model of polycystic ovary syndrome. Pars of Jahrom University of Medical Sciences, 15(1); 23-35.
38.Sadoughi, S.D. (2017). Effect of crocin onBax/Bcl-2 ratio, lipid peroxidation and antioxidant enzymes activity in liver tissue of chick embryo treated with silver nanoparticles. Horizon of Medical Sciences, 23(4); 293-299.
39.Sari-Sarraf, V., Amirsasan, R., Sheikholeslami-Vatani, D., Faraji, H. (2016). Effect of creatine supplementation on the factors involved in apoptosis-related process(Bax, Bcl-2) and their ratio (Bcl-2/Bax) during acute resistance exercise in middle-aged men. Journal of Kurdistan University of Medical Sciences, 21; 17-28 .
40.Sharma, V., Katiyar, A., Agrawal, R.C. (2017). Glycyrrhiza glabra: Chemistry and Pharmacological Activity. Sweeteners, 2017; 87-100.
41.Tahmasbpour, N., Dehghan, G., Hosseinpour Feizi, M.A., Monirinasab, H. (2014). The effect of Teucrium orientale on oxidative stress marker and liver function enzymes in streptozotocin-induced diabetic rats. Armaghan-e- Danesh Magazine, 19(2); 135-145.
42.Wang, K. (2014). Molecular mechanisms of liver injury: Apoptosis or necrosis. Exp Toxicol Pathology, 66(8); 351-356..
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