واکنش ژنوتیپ‌های مختلف لوبیا چشم بلبلی (Vigna sinensis) به ویروس موزاییک معمولی لوبیا (BCMV) در شرایط گلخانه
محورهای موضوعی : گیاه پزشکی
سمانه جوانمردی
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ساسان قاسمی
2
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نوح شهرآیین
3
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کلید واژه: genotype, Cowpea, ژنوتیپ, لوبیا چشم بلبلی, ویروس موزاییک معمولی لوبیا, Bean common mosaic virus,
چکیده مقاله :
ویروس موزاییک معمولی لوبیا (Bean Common Mosaic Virus) دارای گسترش جهانی وسیعی بوده، باعث کاهش عملکرد و همچنین نقصان در کیفیت محصول می گردد. بکارگیری ژنوتیپ‌های متحمل و مقاوم از راهکارهای اصلی مدیریت این بیماری می‌باشد. بیست ژنوتیپ لوبیا چشم بلبلی در دو آزمایش مجزا با اعمال متغیرهای آلودگی و عدم آلودگی در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در شرایط گلخانه مورد بررسی قرار گرفتند. آلودگی در مرحله دو برگی به صورت مکانیکی و ارزیابی ظاهری 21 روز بعد از آلودگی انجام شد. جهت تعیین میزان آلودگی ژنوتیپ‌ها از آزمون الایزا استفاده گردید. آلودگی ژنوتیپ‌های لوبیا چشم بلبلی به وسیله آزمون RT-PCR و با استفاده از آغازگر های اختصاصی BCMV، نیز مورد بررسی قرار گرفت. نتایج بیانگر تکثیر قطعه‌ای به طول 373 جفت باز، در گیاهان آلوده بود. ژنوتیپ‌های مورد بررسی از 66/6 تا 80 درصد آلودگی به BCMV را نشان دادند. با توجه به علائم ایجاد شده در اثر مایه زنی مکانیکی با BCMV به ژنوتیپ‌های مختلف لوبیا چشم بلبلی نمره دهی (4-0) برای به دست آوردن شدت آلودگی صورت پذیرفت. با توجه به نتایج به دست آمده ژنوتیپ‌ها به گروه‌های خیلی حساس، حساس، نیمه مقاوم و مقاوم تقسیم‌بندی شدند.
Bean common mosaic virus is widely spread in the world, and also causes functional impairment in the quality of the product. Using tolerant and resistant genotypes is one of the main approaches to the management of this disease. For this purpose, the present study was conducted in two separate sections. 20 cowpea genotypes were studied in two separate experiments, with and without infection in a randomized complete block design, with three replications in greenhouse. The infection was conducted in 2-leaf phase by mechanical inoculation and morphological evaluation was recorded 21 days after infection. ELISA test was used to determine the infection rate of genotypes. Infection of cowpea genotypes were also checked by RT-PCR test using specific BCMV primers. Results suggest amplified fragment with length of 373 bp in the infected plants. The infection rate of different genotypes was 66.6 to 80%. According to the symptoms caused by mechanical inoculation with BCMV to cowpea genotypes was scored between 0-4 which used to obtain disease severity. According the all results, the genotypes divided to hypersensitive, sensitive, semi-resistant and resistant group.
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