The Effects of Eight Weeks of Submaximal Running and High-fat Diet on IL-6 Gene Expression in the Muscle Tissue of Male Rats
Subject Areas : Immunology and exercise science
Shima
Ebadi
1
(Department of Sports Sciences, Faculty of Humanities and Art, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
Ardeshir
Zafari
2
(Department of Sports Sciences, Faculty of Humanities and Art, Zanjan Branch, Islamic Azad University, Zanjan, Iran)
Keywords: Aerobic training, body weight, High-fat diet, Inter Leukin-6,
Abstract :
It seems that the release of IL-6 from the contracting muscles and accumulation in the blood circulation has a close relationship with the duration and intensity of physical activity. The aime of this experimental study was to determine the effects of eight weeks of submaximal running and high-fat diet on IL-6 gene expression levels in the muscle tissue of an animal model. At the end of the two-week period of keeping under controlled conditions, 40 two-month-old male rats were randomly divided into four equal groups of control, high-fat diet, submaximal running, and a combination of high-fat diet and submaximal running. The training groups participated in the aerobic running protocol on the treadmill for five days a week for eight weeks. The polymerase chain reaction method was used to determine the levels of IL-6 gene expression in muscle tissue. One-way analysis of variance was used to determine between-group differences. The effects of high-fat diet and running with submaximal intensity on IL-6 gene expression in muscle tissue are significant. The consumption of high-fat food causes adverse changes in the expression of IL-6 gene, but eight weeks of running with sub-maximal intensity moderates the adverse effects of high-fat diet and creates favorable changes in the expression of IL-6 gene and body weight.
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