ساخت و بررسی خواص داربست (کیتوسان/ پلی‌وینیل‌پیرولیدون) حاوی کتیرا به روش خشکاندن انجمادی

محورهای موضوعی : بیومواد

حامد قمی ¹ , آزاده سپیانی ² , مرجان میرحاج ³

1 - استادیار، مرکز تحقیقات مواد پیشرفته، دانشکده مهندسی مواد، واحد نجف آباد، دانشگاه آزاد اسلامی، نجف آباد، ایران.
2 - کارشناسی ارشد، مھندسی بافت، واحد نجف آباد ، دانشگاه آزاد اسلامی، نجف آباد، ایران
3 - کارشناسی ارشد،مھندسی پزشکی، دانشگاه آزاد یزد، یزد، ایران

تاریخ دریافت : 1398/05/01 تاریخ پذیرش : 1399/02/13 تاریخ انتشار : 1399/02/13

کلید واژه: کتیرا, کیتوسان, اشرشیاکلی, مهندسی بافت پوست, استافیلوکوک اورئوس,

چکیده مقاله :

کیتوسان ‌بعنوان یک جزء ماتریکس خارج سلولی جهت تهیه داربست متخلخل در مهندسی بافت مورد بررسی قرارگرفته است. در این تحقیق، داربست کیتوسان و داربست کیتوسان/ پلی‌وینیل‌پیرولیدون به‌عنوان شاهد و داربست کامپوزیت (کیتوسان/پلی‌وینیل‌پیرولیدون)/ کتیرا با نسبت‌های 25:75، 50:50 و 75:25 توسط روش خشکاندن انجمادی ساخته شد. اثر کتیرا بر خواص ساختاری و خواص آنتی‌باکتریال در نمونه‌ها موردبررسی قرار گرفت. مورفولوژی سطح، خواص مکانیکی، درصد تخلخل و گروه‌های عاملی بر روی سطح نمونه‌ها با استفاده از میکروسکوپ الکترونی روبشی (SEM)، آزمون استحکام فشاری و FTIR مورد بررسی قرار گرفتند. نتایج نشان داد که درصد تخلخل در داربست حاوی کتیرا نسبت به داربست بدون کتیرا افزایش‌یافته است. با حفظ نمونه‌ها در محلول بافر فسفات (PBS) برای 14 روز، زیست‌تخریب‌پذیری داربست‌ها موردبررسی قرارگرفته شد و نتایج نشان داد میزان تخریب در داربست (کیتوسان / پلی‌وینیل‌پیرولیدون) / کتیرا با نسبت 75:25 افزایش یافت. نتایج نشان داد در داربست کیتوسان / پلی‌وینیل‌پیرولیدون حاوی کتیرا با نسبت (75:25) رشد باکتری استافیلوکوک اورئوس و E.coli کاهش‌یافته است. بنابراین بر اساس نتایج این تحقیق، داربست‌های حاوی کتیرا باعث بهبود خواص آنتی‌باکتریایی می‌گردد.

چکیده انگلیسی:

Chitosan as a component of the extracellular matrix is extensively investigated for preparation of porous scaffolds for tissue engineering. In this study, chitosan and (chitosan/polyvinylpyrrolidone) scaffolds as control and (chitosan/polyvinylpyrrolidone) / tragacanth composite scaffolds with 25:75, 50:50, and 75:25 ratios were fabricated by the freeze-drying method. The effect of tragacanth on the structural and antibacterial properties of the samples was evaluated. Surface morphology, mechanical properties, porosity and functional groups on the surface of the samples were evaluated by scanning electron microscopy (SEM), compressive strength test, and FTIR. The results showed the porosity of scaffolds with tragacanth increased in comparison to scaffolds without tragacanth. Biodegradable behavior of the scaffolds was examined by retaining the samples in phosphate buffer solution (PBS) for 14 days and the results showed an increase in the degradation of the (chitosan/polyvinylpyrrolidone) / tragacanth scaffold with the ratio of 75:25. The results showed decreased growth of E.coli and Staphylococcus aureus bacteria in the presence of three-component scaffold with tragacanth. Therefore, according to the results of this study, tragacanth containing scaffolds improve antibacterial properties.

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