ساخت و مشخصه یابی هیدروژل پلی‌وینیل الکل/ آلوورا با روش ریخته گری حلال جهت کاربرد به عنوان زخم‌پوش

محورهای موضوعی : بیومواد

محبوبه محمودی ¹ (مدیرگروه کارشناسی ارشد مهندسی پزشکی دانشگاه ازاد اسلامی واحد یزد)
مونا حاجیان ² (دانشجو)
رعنا ایمانی ³ (هیآت علمی گروه مهندسی پزشکی دانشگاه صنعتی امیرکبیر)

تاریخ دریافت : 1394/09/24 تاریخ پذیرش : 1396/09/19 تاریخ انتشار : 1396/09/01

کلید واژه: "آلوورا", " پلی‌وینیل الکل", "زخم‌پوش", "ریخته‌گری حلال", "فیلم‌های هیدروژلی",

چکیده مقاله :

در این مطالعه، فیلم‌های هیدروژلی پلی‌وینیل الکل/ آلوورا با درصدهای وزنی مختلف آلوورا (20،30و50 درصد) توسط روش ریخته‌گری حلال ساخته شد. سپس میزان جذب آب، نرخ تخریب، میزان عبور بخار آب و خواص مکانیکی فیلم‌ها مورد ارزیابی قرار گرفت. طیف سنجی مادون قرمز تبدیل فوریه (FTIR) و میکروسکوپ الکترونی روبشی (SEM) به ترتیب برای شناسایی پیوندهای تشکیل شده در نمونه‌ها و بررسی مورفولوژی سطح آن‌ها انجام گرفت. از نتایج آزمایش‌ها مشاهده گردید که با افزایش درصد وزنی آلوورا تا 30%، میزان جذب آب، نرخ تخریب، خواص مکانیکی و تعداد تخلخل‌های موجود در سطح نمونه‌ها افزایش می‌یابد. همچنین، افزایش نرخ عبور بخار آب از نمونه‌ها با افزایش درصد وزنی آلوورا مشاهده گردید. در نهایت توسط آزمون‌های برون‌تنی (In vitro)، سمیت سلولی و مورفولوژی سلول‌های فیبروبلاست (L929) بر روی سطح نمونه‌ها مورد بررسی و ارزیابی قرار گرفت. از نتایج این آزمون‌، افزایش رشد و تکثیر سلول‌ها بر روی نمونه پلی‌وینیل الکل/ آلوورا با درصد وزنی 30% آلوورا مشاهده گردید. بنابراین هیدروژل پلی‌وینیل الکل/ آلوورا می‌تواند گزینه مناسبی برای کاربرد به عنوان زخم‌پوش باشد.

چکیده انگلیسی:

In this study, we have tried to make hydrogel films by polyvinyl alcohol and Aloe vera which used in treat wounds. The films were prepared by solvent casting method with different concentration of aloe vera include 20, 30 and 50%. Some properties of samples such as water absorption, degradation rate, water vapour transmission rate and mechanical properties were examined. Also, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) test were carried out in order to identify the bonds and morphological assessment in the samples, respectively. The results showed that with increasing of aloe vera concentration to 30% (PA30), water absorption rate, degradation rate and mechanical properties are increased. In according to SEM investigations, we found with aloe vera increasing, the porosity on surface of samples was increased. In the other hand, water vapour transmission rate have direct comparison with percentage of aloe vera concentration. Finally, cell cytotoxicity and morphology fibroblast cells (L929) attached on the samples surface evaluated by in vitro assay. The results showed that cells proliferated in greater numbers on the PA30 sample compared to other samples. Therefore, our results indicated that polyvinyl alcohol/Aloe vera hydrogel films are a good choice for use in wound dressing.

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