تأثیر عصاره چای سبز بر آسیب‌شناسی بافتی و شاخص‌های سرمی آسیب بافت کبد در موش‌های صحرایی دیابتی شده با استرپتوزوتوسین

محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکی

علی اکبر ابوالفتحی ¹ , علی رضایی ² , غفور موسوی ³ , محمدرضا ولیلو ⁴ , بهبود جعفری ⁵

1 - دانشگاه آزاد اسلامی، واحد اهر، گروه زیست شناسی، اهر، ایران
2 - دانشگاه آزاد اسلامی، واحد تبریز، گروه علوم درمانگاهی، تبریز، ایران
3 - دانشگاه آزاد اسلامی، واحد تبریز، گروه علوم درمانگاهی، تبریز، ایران
4 - دانشگاه آزاد اسلامی، واحد شبستر، گروه دامپزشکی، شبستر، ایران
5 - دانشگاه آزاد اسلامی، واحد اهر، گروه میکروبیولوژی، اهر، ایران

تاریخ دریافت : 1390/07/04 تاریخ پذیرش : 1390/10/10 تاریخ انتشار : 1390/09/01

کلید واژه: کبد, موش صحرایی, دیابت, چای سبز, شاخص‌های آسیب کبد, آسیب‌شناسی بافتی,

چکیده مقاله :

هدف از مطالعه حاضر ارزیابی آسیب کبد در دیابت ملیتوس و بررسی اثرات محافظتی عصاره چای سبز در موش های صحرایی دیابتی شده توسط استرپتوزوتوسین می باشد. برای این منظور، 40 سر مو ش صحرایی نر به 4 گروه 10 تایی تقسیم شدند: 1) گروه شاهد سالم، 2) گروه سالم تیمار با عصاره چای سبز، 3) گروه دیابتی و 4) گروه دیابتی تیمار با عصاره چای سبز. برای دیابتی کردن موش‌ها، استرپتوزوتوسین با تک دز mg/kg 65 به ‌صورت داخل صفاقی تزریق شد. از زمان شروع آزمایش، موش های تیمار با عصاره، عصاره چای سبز (w/v ،5/1%) را در آب آشامیدنی به مدت 8 هفته دریافت کردند. در انتهای آزمایش سطوح سرمی آلانین آمینوترانسفراز (ALT)، آسپارتات آمینوترانسفراز(AST)، آلکالین فسفاتاز (AP)، آلبومین و بیلی روبین تام اندازه گیری شدند. برای آسیب شناسی بافت کبد نیز برش هایی با رنگ آمیزی هماتوکسیلین-ائوزین، طبق روش های معمول تهیه مقاطع هیستوپاتولوژی تهیه گردید. موش های صحرایی دیابتی افزایش معنی دار شاخص های سرمی آسیب بافت کبد را در مقایسه با گروه شاهد نشان دادند (05/0p<)، در حالی که در گروه دیابتی تیمار با عصاره چای سبز، این شاخص ها در مقایسه با گروه دیابتی به طور معنی داری کاهش نشان داد (05/0p<). یافته های آسیب شناسی بافت کبد در توافق با نتایج بیوشیمیایی سرم نشانگر بهبود ساختار بافت آسیب دیده کبد در موش های دیابتی بود. نتایجاینمطالعهنشانداد عصاره چای سبز دارایاثراتمحافظتاز بافت کبد در دیابت القاء شده با استرپتوزوتوسین می باشد.

چکیده انگلیسی:

The aim of present study was to evaluate the liver injury as a complication of diabetes mellitus and to assess the hepatoprotective properties of Green tea extract in streptozotocin-induced diabetic rats. For this purpose, 40 male Wistar rats were randomly separated into four groups, each containing 10 animals: Group 1, healthy control rats; Group 2, normal rats treated with Green tea extract (1.5%, w/v) was given in drinking water; Group 3, diabetic rats and Group 4, diabetic rats treated with Green tea extract (1.5%, w/v) in drinking water. The extract was injected in intraperitoneal route for a period of 8 weeks. Control groups received normal saline in similar manner. Diabetes was induced by single injection of STZ (75 mg/kg i.p.). At the end of experiment, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), albumin and bilirubin were measured. For histopathological evaluation, tissue specimens were fixed in 10% buffered formalin and 5 micron thick sections with H&E stain were prepared using routine hitopathological techniques. In diabetic rats, serum levels of functional liver markers were found to be significantly increased in comparison with control group (p<0.05), while this markers in diabetic rats treated with Green tea extract  significantly decreased as compared to diabetic rats. Histopathological findings were in consistent with biochemical results. The data obtained proved that Green tea extract has hepatoprotective activity against diabetic hepatopathy in streptozotocin-induced diabetic rats.

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