جستجوی رئوویروس‌ها در موارد پیش‌معده التهابی جوجه‌های گوشتی استان اصفهان به روش مولکولی RT-PCR و ارزیابی پاتولوژیکی ضایعات مربوطه
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
سارا کریمی
1
(دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.)
مجید غلامی آهنگران
2
(دانشیار علوم درمانگاهی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.)
تاریخ دریافت : 1401/07/19
تاریخ پذیرش : 1401/11/30
تاریخ انتشار : 1401/11/01
کلید واژه:
هیستوپاتولوژی,
جوجه گوشتی,
رئوویروس,
التهاب پیش‌معده,
ردیابی مولکولی,
چکیده مقاله :
التهاب پیش‌معده با بزرگ‌شدگی این ارگان مشخص می‌شود و عوامل متعدد عفونی و غیر عفونی در ایجاد آن نقش دارند و از جمله این عوامل رئوویروس‌ها، هستند. براساس شواهد درمانگاهی و گزارشاتی از شیوع رئوویروس‌ها در مزارع پرورش طیور در ایران، می‌توان بزرگ شدگی و التهاب پیش‌معده در جوجه‌های گوشتی را با عفونت‌های رئوویروسی مرتبط دانست. در بررسی حاضر، به منظور شناسایی و ردیابی رئوویروس ها در بافت پیش‌معده، ژن S1 به روش RT-PCR (Reverse Transcription Polymerase Chain Reaction) مورد تکثیر قرار گرفت. براساس یافته ها، از 75 نمونه پیش‌معده جمع‌آوری شده از 15 فارم جوجه گوشتی استان اصفهان با علایم بزرگی و التهاب پیش‌معده، 46 نمونه (33/61 درصد نمونه ها) و 10 فارم (66/66 درصد فارم های بررسی شده)، بر اساس تکثیر قطعه 532 جفت بازی مربوط به ژن S1 ، از نظر حضور رئوویروس ها مثبت ارزیابی شدند. همچنین پیش‌معده‌های جمع آوری شده، همگی از لحاظ ماکروسکوپی بزرگ‌تر از حالت عادی بوده و ضخامت بیشتری داشتند. ناحیه اتصال به سنگدان هم شل و فاقد قوام کافی بود. علاوه بر آن، بافت مخاطی پیش‌معده لزج و حاوی ترشحات زرد رنگ بود. در تمامی مقاطع هیستوپاتولوژی مربوط به نمونه‌های مثبت از لحاظ رئوویروس نیز، نکروز به همراه سلول‌های التهابی در غدد سطحی و عمقی پیش‌معده قابل مشاهده بود. به نظر می‌رسد آلودگی با رئوویروس ها در جوجه‌های گوشتی استان اصفهان وجود دارد و این آلودگی می‌تواند در ایجاد عارضه التهاب و بزرگ‌شدگی پیش‌معده نقش داشته باشد. لذا اقدامات لازم برای کنترل و پیشگیری از این آلودگی در جوجه‌های گوشتی ضروری است.
چکیده انگلیسی:
Proventriculitis is a disease characterized by enlargement of the proventriculus. Several factors including infectious and non-infectious agents play a role in its development. Reoviruses could be considered as a main causal agent of proventriculitis in chickens. Based on clinical evidence and reports of the prevalence of reovirus infection in poultry farms in Iran, the enlargement of proventriculus and proventriculitis may be related to the reovirus infections. In the present study, to detect reoviruses in the proventriculus samples, the S1 gene was amplified by RT-PCR (Reverse Transcription-Polymerase Chain Reaction). According to the findings, out of 75 proventriculus samples collected from 15 broiler farms with large proventriculus in Isfahan province, 46 samples (61.33% of the samples) and 10 farms (66.66% of the studied farms) were positive for reovirus based on the amplification of the 532 bp fragment related to the S1 gene. The collected proventriculi were all macroscopically larger than normal and had more thickness. The area connecting the proventriculus to the gizzard was loose and lacked sufficient consistency. In addition, the mucosal layer of the proventriculus was slimy and contained yellow secretions. In all the histopathological sections related to positive samples, necrosis along with inflammatory cells were visible in the superficial and deep glands of the proventriculus. In general, it seems that there is infection with reoviruses in broiler chickens of Isfahan province and this infection can play a role in causing the inflammation and enlargement of the proventriculus, therefore, necessary measures should be taken to control and prevent this infection in broiler chickens.
منابع و مأخذ:
Allawe, A.B., Abbas, A.A. and Taha, Z.H. (2017). Detection of transmissible viral proventriculitis in Iraq. Journal of Entomology and Zoology Studies, 5 (3): 974-978.
Asadi, J., Gholami-Ahangaran, M. and Momtaz, H. (2018). Molecular detection of Ornithobacterium rhinotracheale in quails of Isfahan province. Veterinary Clinical Pathology, 12(45): 1-7. [In Persian]
Al-Ebshahy, E., Mohamed, S. and Abas, O. (2020). First report of seroprevalence and genetic characterization of avian orthoreovirus in Egypt. Journal of Tropical Animal Health and Productio, 52 (2): 1049-1054.
Bancroft, J.D. and Gamble, M. (2008). Theory and practice of histological techniques. 6th ed., Churchill Livingstone: Elsevier Health Sciences, pp: 100-110.
Benavente, J. and Martínez-Costas, J. (2007). Avian reovirus: structure and biology. Virus Research, 123(2): 105-119.
Cătană, N., Coman, A. and Fodor, I. (2012). Research on the role of avian reovirus in transmissible viral etiology proventriculitis. Lucrari Stiintifice-Universitatea de Stiinte Agricole a Banatului Timisoara, Medicina Veterinara, 45(3): 23-26.
Dormitorio, T.V, Giambrone, J.J. and Hoerr, F.J. (2007). Transmissible proventriculitis in broilers. Avian Pathology, 36(2): 87-91.
Li, G., Yuan, S., He, M., Zhao, M., Hao, X., Song, M., et al. (2018). Emergence of gyrovirus 3 in commercial broiler chickens with transmissible viral proventriculitis. The 16th World Veterinary Poultry Association Transboundary and Emerging Diseases, 65(5): 1170-1174.
Gholami-Ahangaran, M., Moghtadaiee, E. and Ahmadi, A. (2019). Molecular detection of Marek,s virus in backyard fowl with nodular lesions in visceral organs. Veterinary Clinical Pathology, 13(50): 151-161. [In Persian]
Grau-Roma, L., Schock, A., Nofrarías, M., Ali Wali, N., de Fraga, A.P., Garcia-Rueda, C., et al. (2020). Retrospective study on transmissible viral proventriculitis and chicken proventricular necrosis virus (CPNV) in the UK. Avian Pathology, 49(1): 99-105.
Guy, J.S. (1998). Virus infections of the gastrointestinal tract of poultry. Poultry Science, 77(8): 1166-1175.
Hafner, S. and Guy, J.S. (2013). Proventriculitis and proventricular dilatation of broiler chickens. In: Diseases of Poultry. Swayne, D.E., Glisson, J.R., McDouglald, L.R., Nolan, L.K., Suarez, D.L. and Nair, V.L. editors. 13th ed., Ames, IA: Wiley-Blackwell Publishing, pp: 1328-1332.
Hauck, R., Stoute, S., Senties-Cue, C.G., Guy, J.S. and Shivaprasad, H. L. (2020). A retrospective study of transmissible viral proventriculitis in broiler chickens in California: 2000–18. Avian Diseases, 64(4): 525-531.
Hedayati, M., Shojadoust, B. and Peyghambari, M. (2012). Identification of reoviruses causing tenosynovitis in birds from Iranian mother hen flocks by RT-PCR and RFLP methods. Iranian Journal of Veterinary Medicine, 7(2): 135-142.
Jeong, J.K., Jeong, H., An, E. and Han, S. (2019). The prevalence of avian reovirus infection in poultry farms of Jeonbuk province, Korea. Korean Journal of Veterinary Service, 42(4): 237-243.
Jones, R.C. and Georgiou, K. (1984). Reovirus‐induced tenosynovitis in chickens the influence of age at infection. Avian Pathology, 13(3): 441-457.
Kim, H.R., Yoon, S.J., Lee, H.S. and Kwon, Y.K. (2015). Identification of a picornavirus from chickens with transmissible viral proventriculitis using metagenomic analysis. Archives of Virology, 160(3): 701-709.
Kouwenhoven, B., Vertommen, M. and Goren, E. (1988). Investigations into the role of reovirus in the malabsorption syndrome. Avian Pathology, 17(4): 879-892.
Kutkat, M.A., Hoda, M.A., Khalil, S.A., El-Fatah, M.A. and Torky, H.A. (2010). Studies on proventriculitis in broilers with molecular characterization of its viral causes. Journal of American Science, 6(9): 582-592.
Lenz, S.D., Hoerr, F.J., Ellis, A.C., Toivio-Kinnucan, M.A. and Yu, M. (1998). Gastrointestinal pathogenicity of adenoviruses and reoviruses isolated from broiler chickens in Alabama. Journal of Veterinary Diagnostic Investigation, 10(2): 145-151.
Marusak, R.A., West, M.A., Davis, J.F., Fletcher, O.J. and Guy, J.S. (2012). Transmissible viral proventriculitis identified in broiler breeder and layer hens. Avian Diseases, 56(4): 757-759.
Mohamed, S., Al-Ebshahy, E.M., Khalil, S.A., AbdelHady, H.A. and Elbestawy, A.R. (2019). Molecular identification and pathogenicity assessment of avian reovirus in Egypt. Alexandria Journal for Veterinary Sciences, 63(1): 100-110.
Pantin-Jackwood, M.J., Brown, T.P. and Huff, G.R. (2004). Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Veterinary Pathology, 41(6): 641-648.
Robertson, M.D. and Wilcox, G.E. (1986). Avian reovirus. Veterinary Bulletin, 56 (4): 154-174.
Sellers, H.S. (2017). Current limitations in control of viral arthritis and tenosynovitis caused by avian reoviruses in commercial poultry. Veterinary Microbiology, 206: 152-156.
Sellers, H.S. (2022). Avian reoviruses from clinical cases of tenosynovitis: An overview of diagnostic approaches and 10-year review of isolations and genetic characterization. Avian Diseases, 66(4): 420-426.
Shabani, A.A., Gholami-Ahangaran, M. and Momtaz, H. (2017). Molecular detection of Ornithbacterium rhinotracheale and Newcastle disease virus in ostriches of Isfahan province. Veterinary Clinical Pathology, 11(42): 97-105. [In Persian]
Souza, S.O., De Carli, S., Lunge, V.R., Ikuta, N., Canal, C.W., Pavarini, S.P., et al. (2018). Pathological and molecular findings of avian reoviruses from clinical cases of tenosynovitis in poultry flocks from Brazil. Poultry Science, 97(10): 3550-3555.
Troxler, S., Rigomier, P., Bilic, I., Liebhart, D., Prokofieva, I., Robineau, B., et al. (2013). Identification of a new reovirus causing substantial losses in broiler production in France, despite routine vaccination of breeders. Veterinary Record, 172(21): 556-556.
Xie, Z., Fadl, A.A., Girshick, T. and Khan, M.I. (1997). Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction. Avian Diseases, 18(2): 654-660.
Yan, T., Li, G., Zhou, D., Hu, L., Hao, X., Li, R. and Cheng, Z. (2022). Long read sequencing revealed proventricular virome of broiler chicken with transmission viral proventriculitis. BMC Veterinary Research, 18(1): 1-7.
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Allawe, A.B., Abbas, A.A. and Taha, Z.H. (2017). Detection of transmissible viral proventriculitis in Iraq. Journal of Entomology and Zoology Studies, 5 (3): 974-978.
Asadi, J., Gholami-Ahangaran, M. and Momtaz, H. (2018). Molecular detection of Ornithobacterium rhinotracheale in quails of Isfahan province. Veterinary Clinical Pathology, 12(45): 1-7. [In Persian]
Al-Ebshahy, E., Mohamed, S. and Abas, O. (2020). First report of seroprevalence and genetic characterization of avian orthoreovirus in Egypt. Journal of Tropical Animal Health and Productio, 52 (2): 1049-1054.
Bancroft, J.D. and Gamble, M. (2008). Theory and practice of histological techniques. 6th ed., Churchill Livingstone: Elsevier Health Sciences, pp: 100-110.
Benavente, J. and Martínez-Costas, J. (2007). Avian reovirus: structure and biology. Virus Research, 123(2): 105-119.
Cătană, N., Coman, A. and Fodor, I. (2012). Research on the role of avian reovirus in transmissible viral etiology proventriculitis. Lucrari Stiintifice-Universitatea de Stiinte Agricole a Banatului Timisoara, Medicina Veterinara, 45(3): 23-26.
Dormitorio, T.V, Giambrone, J.J. and Hoerr, F.J. (2007). Transmissible proventriculitis in broilers. Avian Pathology, 36(2): 87-91.
Li, G., Yuan, S., He, M., Zhao, M., Hao, X., Song, M., et al. (2018). Emergence of gyrovirus 3 in commercial broiler chickens with transmissible viral proventriculitis. The 16th World Veterinary Poultry Association Transboundary and Emerging Diseases, 65(5): 1170-1174.
Gholami-Ahangaran, M., Moghtadaiee, E. and Ahmadi, A. (2019). Molecular detection of Marek,s virus in backyard fowl with nodular lesions in visceral organs. Veterinary Clinical Pathology, 13(50): 151-161. [In Persian]
Grau-Roma, L., Schock, A., Nofrarías, M., Ali Wali, N., de Fraga, A.P., Garcia-Rueda, C., et al. (2020). Retrospective study on transmissible viral proventriculitis and chicken proventricular necrosis virus (CPNV) in the UK. Avian Pathology, 49(1): 99-105.
Guy, J.S. (1998). Virus infections of the gastrointestinal tract of poultry. Poultry Science, 77(8): 1166-1175.
Hafner, S. and Guy, J.S. (2013). Proventriculitis and proventricular dilatation of broiler chickens. In: Diseases of Poultry. Swayne, D.E., Glisson, J.R., McDouglald, L.R., Nolan, L.K., Suarez, D.L. and Nair, V.L. editors. 13th ed., Ames, IA: Wiley-Blackwell Publishing, pp: 1328-1332.
Hauck, R., Stoute, S., Senties-Cue, C.G., Guy, J.S. and Shivaprasad, H. L. (2020). A retrospective study of transmissible viral proventriculitis in broiler chickens in California: 2000–18. Avian Diseases, 64(4): 525-531.
Hedayati, M., Shojadoust, B. and Peyghambari, M. (2012). Identification of reoviruses causing tenosynovitis in birds from Iranian mother hen flocks by RT-PCR and RFLP methods. Iranian Journal of Veterinary Medicine, 7(2): 135-142.
Jeong, J.K., Jeong, H., An, E. and Han, S. (2019). The prevalence of avian reovirus infection in poultry farms of Jeonbuk province, Korea. Korean Journal of Veterinary Service, 42(4): 237-243.
Jones, R.C. and Georgiou, K. (1984). Reovirus‐induced tenosynovitis in chickens the influence of age at infection. Avian Pathology, 13(3): 441-457.
Kim, H.R., Yoon, S.J., Lee, H.S. and Kwon, Y.K. (2015). Identification of a picornavirus from chickens with transmissible viral proventriculitis using metagenomic analysis. Archives of Virology, 160(3): 701-709.
Kouwenhoven, B., Vertommen, M. and Goren, E. (1988). Investigations into the role of reovirus in the malabsorption syndrome. Avian Pathology, 17(4): 879-892.
Kutkat, M.A., Hoda, M.A., Khalil, S.A., El-Fatah, M.A. and Torky, H.A. (2010). Studies on proventriculitis in broilers with molecular characterization of its viral causes. Journal of American Science, 6(9): 582-592.
Lenz, S.D., Hoerr, F.J., Ellis, A.C., Toivio-Kinnucan, M.A. and Yu, M. (1998). Gastrointestinal pathogenicity of adenoviruses and reoviruses isolated from broiler chickens in Alabama. Journal of Veterinary Diagnostic Investigation, 10(2): 145-151.
Marusak, R.A., West, M.A., Davis, J.F., Fletcher, O.J. and Guy, J.S. (2012). Transmissible viral proventriculitis identified in broiler breeder and layer hens. Avian Diseases, 56(4): 757-759.
Mohamed, S., Al-Ebshahy, E.M., Khalil, S.A., AbdelHady, H.A. and Elbestawy, A.R. (2019). Molecular identification and pathogenicity assessment of avian reovirus in Egypt. Alexandria Journal for Veterinary Sciences, 63(1): 100-110.
Pantin-Jackwood, M.J., Brown, T.P. and Huff, G.R. (2004). Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Veterinary Pathology, 41(6): 641-648.
Robertson, M.D. and Wilcox, G.E. (1986). Avian reovirus. Veterinary Bulletin, 56 (4): 154-174.
Sellers, H.S. (2017). Current limitations in control of viral arthritis and tenosynovitis caused by avian reoviruses in commercial poultry. Veterinary Microbiology, 206: 152-156.
Sellers, H.S. (2022). Avian reoviruses from clinical cases of tenosynovitis: An overview of diagnostic approaches and 10-year review of isolations and genetic characterization. Avian Diseases, 66(4): 420-426.
Shabani, A.A., Gholami-Ahangaran, M. and Momtaz, H. (2017). Molecular detection of Ornithbacterium rhinotracheale and Newcastle disease virus in ostriches of Isfahan province. Veterinary Clinical Pathology, 11(42): 97-105. [In Persian]
Souza, S.O., De Carli, S., Lunge, V.R., Ikuta, N., Canal, C.W., Pavarini, S.P., et al. (2018). Pathological and molecular findings of avian reoviruses from clinical cases of tenosynovitis in poultry flocks from Brazil. Poultry Science, 97(10): 3550-3555.
Troxler, S., Rigomier, P., Bilic, I., Liebhart, D., Prokofieva, I., Robineau, B., et al. (2013). Identification of a new reovirus causing substantial losses in broiler production in France, despite routine vaccination of breeders. Veterinary Record, 172(21): 556-556.
Xie, Z., Fadl, A.A., Girshick, T. and Khan, M.I. (1997). Amplification of avian reovirus RNA using the reverse transcriptase-polymerase chain reaction. Avian Diseases, 18(2): 654-660.
Yan, T., Li, G., Zhou, D., Hu, L., Hao, X., Li, R. and Cheng, Z. (2022). Long read sequencing revealed proventricular virome of broiler chicken with transmission viral proventriculitis. BMC Veterinary Research, 18(1): 1-7.
