تاثیر کمبود متیونین جیره بر بافت‌شناسی روده باریک بلدرچین ژاپنی

محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکی

اشکان خلخالی ¹ , سمیه حامدی ² , محمدرضا پریانی ³

1 - دانشجوی دکترای حرفه‌ای، رشته دامپزشکی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران.
2 - استادیار گروه علوم پایه ، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران.
3 - دانشیار گروه علوم پایه ، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران.

تاریخ دریافت : 1397/08/20 تاریخ پذیرش : 1398/09/03 تاریخ انتشار : 1398/11/01

کلید واژه: بلدرچین ژاپنی, روده باریک, بافت‌شناسی, کمبود متیونین,

چکیده مقاله :

به منظور مطالعه تاثیرکمبود متیونین جیرهبه عنوان یک اسیدآمینه حیاتی بر بافت روده باریک،تعداد 20 قطعه بلدرچین ژاپنی نر یک‌روزه به صورت تصادفی به 2 گروه 10تایی تقسیم شدند. بلدرچین‌های گروه آزمایشیباجیره فاقد مکمل متیونین و گروه شاهد با جیره استاندارد بلدرچین به مدت 6 هفته تغذیه شدند.پس از پایان دوره و کشتار بلدرچین‌هانمونه‌های بافت‌شناسی ازقسمت میانیدوازدهه، تهی روده و ایلئوم تهیه و مراحل معمول بافت‌شناسی انجام پذیرفت. سپسبرش های 6 میکرونی تهیه شده،تحت رنگ آمیزی هماتوکسیلین-ائوزین و روش هیستوشیمی پریودیک اسید چیف قرار گرفتند. با استفاده از نرم افزارAxio vixion Rel 4.8ارتفاع و عرض کرک، عمق کریپت، نسبت ارتفاع کرک به عمق کریپت و تعداد سلول های جامی در واحد سطح اندازه‌گیری و داده‌ها به صورت میانگین±انحراف معیار ارائه شده و با روش آماریIndependent-Samples t-Testو در سطح معنی p < 0/05 ;از لحاظ آماری مورد مقایسه قرار گرفتند. کاهش متیونین جیره سبب کاهش معنی‌دار عرض کرک و تعداد سلول‌های جامی در هر سه قسمت روده باریک و نیز کاهش معنی‌دار ارتفاع کرک و نسبت ارتفاع کرک به عمق کریپت در تهی‌روده شد. باتوجه به این که کاهش نسبت ارتفاع کرک به عمق کریپت سبب کاهش سطح جذب در روده می‌شود،مشخص گردید که کاهش متیونین جیره با اثر منفی بر سلول های جامی و کاهش ترشحات مخاطی سبب آسیب به مخاط روده باریک و همچنین کاهش سطح هضم و جذب روده ای و در نهایت کاهش وزن بلدرچین می‌شود.

چکیده انگلیسی:

Background and purpose: Japanese quail (Coturnix japonica) is a small size bird with big economical profits. This species is a good "dual-purpose" bird and is now reared for the meat and egg production. Quails have relatively early maturity and may be used as an experimental model due to their fast development, small size, etc. Moreover, because of high mortality due to emergence of new and reemergence of existing diseases in chickens, quails are being reared as they are more resistant to common poultry diseases. Methionine (Met) is a sulfur-containing amino acid with the biological functions including involvement in synthesis of eukaryotic proteins, defense against oxidative stress, methylation reactions and so on. Met also has a role in avian immune function and the nutrient digestibility in the intestine. Changes in small intestine morphology can alter absorption rate, weight gain and performance of the animal because of its important role in digestive tract for absorption. From the other point of view, alteration in the ingredients of diet may lead to a change in the intestinal mucosa and subsequently alteration in poultry performance. Thus, the aims of this study were to investigate the effect of Met deficiency on the development of the small intestine (duodenum, jejunum and ileum) and the goblet cell population in Japanese quails.Materials and methods: To evaluate the Met deficiency as a vital amino acid on histology of small intestine of Japanese quail, 20 male one-day old quails were randomly allocated into 2 groups of 10 birds. One group received Met deficient diet while another group of birds were kept as control with standard diet for 6 weeks. At the end of the experiment, all animals sacrificed by cervical dislocation. Whole length small intestine was removed immediately and immersed in 10% buffered formalin. After fixation, 1cm-thick samples were taken from the middle parts of duodenum (from the gizzard to pancreatic and bile duct), jejunum (from the bile duct to Meckel’s diverticulum) and ileum (from the Meckel’s diverticulum to ileo-cecal-colonic junction). After routine histological laboratory methods, 6μm-thick transverse cross-sections were made, a total number of 10 sections used from each intestinal segment of each bird; sections stained with Haematoxylin and Eosin and Periodic acid-Schiff. For measuring length and width of villi, depth of crypts and goblet cells number Axio vixion Rel 4.8 software were used. Data were expressed as Mean±SD. Data analysis was performed by Independent-Samples T Test method and differences considered statistically significant at p < /p>

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